LOCUS       NZ_CP116092             6647 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Escherichia coli strain DETEC-S565 plasmid pDETEC75, complete
            sequence.
ACCESSION   NZ_CP116092
VERSION     NZ_CP116092.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31988496
            Assembly: GCF_027944655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Extended-spectrum beta-lactamase genes traverse the Escherichia coli
            populations of ICU patients, staff and environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2023) Institute of Microbiology and Infection,
            University of Birmingham, Institute of Microbiology and Infection,
            Birmingham B15 2TT, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 02:39:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,104
            CDSs (total)                      :: 4,983
            Genes (coding)                    :: 4,803
            CDSs (with protein)               :: 4,803
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 180
            CDSs (without protein)            :: 180
            Pseudo Genes (ambiguous residues) :: 0 of 180
            Pseudo Genes (frameshifted)       :: 74 of 180
            Pseudo Genes (incomplete)         :: 111 of 180
            Pseudo Genes (internal stop)      :: 34 of 180
            Pseudo Genes (multiple problems)  :: 36 of 180
            Pseudo Genes (short protein)      :: 2 of 180
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116092.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DETEC-S565"
                     /isolation_source="ICU staff rectal swab"
                     /db_xref="taxon:562"
                     /plasmid="pDETEC75"
                     /geo_loc_name="China: Guangzhou"
                     /collection_date="2019-09-01"
     gene            14..1582
                     /gene="cea"
                     /locus_tag="PIC22_RS25400"
                     /old_locus_tag="PIC22_25395"
     CDS             14..1582
                     /gene="cea"
                     /locus_tag="PIC22_RS25400"
                     /old_locus_tag="PIC22_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1968..2105
                     /locus_tag="PIC22_RS25405"
                     /old_locus_tag="PIC22_25400"
     CDS             1968..2105
                     /locus_tag="PIC22_RS25405"
                     /old_locus_tag="PIC22_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2304..2462)
                     /locus_tag="PIC22_RS25550"
     CDS             complement(2304..2462)
                     /locus_tag="PIC22_RS25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(3132..3323)
                     /locus_tag="PIC22_RS25410"
                     /old_locus_tag="PIC22_25405"
     CDS             complement(3132..3323)
                     /locus_tag="PIC22_RS25410"
                     /old_locus_tag="PIC22_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3387..3710
                     /locus_tag="PIC22_RS25415"
                     /old_locus_tag="PIC22_25410"
     CDS             3387..3710
                     /locus_tag="PIC22_RS25415"
                     /old_locus_tag="PIC22_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3919..>4350
                     /locus_tag="PIC22_RS25420"
                     /old_locus_tag="PIC22_25415"
                     /pseudo=""
     CDS             3919..>4350
                     /locus_tag="PIC22_RS25420"
                     /old_locus_tag="PIC22_25415"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4388..4906
                     /locus_tag="PIC22_RS25425"
                     /old_locus_tag="PIC22_25420"
     CDS             4388..4906
                     /locus_tag="PIC22_RS25425"
                     /old_locus_tag="PIC22_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4913..5146
                     /locus_tag="PIC22_RS25430"
                     /old_locus_tag="PIC22_25425"
     CDS             4913..5146
                     /locus_tag="PIC22_RS25430"
                     /old_locus_tag="PIC22_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5464..5883)
                     /locus_tag="PIC22_RS25435"
                     /old_locus_tag="PIC22_25430"
     CDS             complement(5464..5883)
                     /locus_tag="PIC22_RS25435"
                     /old_locus_tag="PIC22_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5964..6401)
                     /locus_tag="PIC22_RS25440"
                     /old_locus_tag="PIC22_25435"
     CDS             complement(5964..6401)
                     /locus_tag="PIC22_RS25440"
                     /old_locus_tag="PIC22_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2960..3047
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2391..2946)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3701..5250
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaggatttt ataatggaaa ccgcggtagc gtactataaa gatggtgttc cttatgatga
       61 taagggacag gtaattatta ctcttttgaa tggtactcct gacgggagtg gctctggcgg
      121 cggaggtgga aaaggaggca gtaaaagtga aagttctgca gctattcatg caactgctaa
      181 atggtctact gctcaattaa agaaaacaca ggcagagcag gctgcccggg caaaagctgc
      241 agcggaagca caggcgaaag caaaggcaaa cagggatgcg ctgactcagc gcctgaagga
      301 tatcgtgaat gaggctcttc gtcacaatgc ctcacgtacg ccttcagcaa cagagcttgc
      361 tcatgctaat aatgcagcta tgcaggcgga agcagagcgt ttgcgccttg cgaaagcaga
      421 agaaaaagcc cgtaaagaag cggaagcagc agaaaaggct tttcaggaag cagaacaacg
      481 acgtaaagag attgaacggg agaaggctga aacagaacgc cagttgaaac tggctgaagc
      541 tgaagagaaa cgactggctg cattgagtga agaagctaaa gctgttgaga tcgcccaaaa
      601 aaaactttct gctgcacaat ctgaagtggt gaaaatggat ggagagatta agactctcaa
      661 ttctcgttta agctccagta tccatgcccg tgatgcagaa atgaaaacgc tcgctggaaa
      721 acgaaatgaa ctggctcagg catccgctaa atataaagaa ctggatgagc tggtcaaaaa
      781 actatcacca agagccaatg atccgcttca gaaccgtcct ttttttgaag caaccagacg
      841 acgggttggg gccggtaaga ttagagaaga aaaacaaaaa caggtaacag catcagaaac
      901 acgtattaac cggataaatg ctgatataac tcagatccag aaggctattt ctcaggtcag
      961 taataatcgt aatgccggta tcgctcgtgt tcatgaagct gaagaaaatt tgaaaaaagc
     1021 acagaataat ctccttaatt cacagattaa ggatgctgtt gatgcaacag ttagctttta
     1081 tcaaacgctg actgaaaaat atggtgaaaa atattcgaaa atggcacagg aacttgctga
     1141 taagtctaaa ggtaagaaaa tcggcaatgt gaatgaagct ctcgctgctt ttgaaaaata
     1201 caaggatgtt ttaaataaga aattcagcaa agccgatcgt gatgctattt ttaatgcgtt
     1261 ggcatcggtg aagtatgatg actgggctaa acatttagat cagtttgcca agtacttgaa
     1321 gattacgggg catgtttctt ttggatatga tgtggtatct gatatcctaa aaattaagga
     1381 tacaggtgac tggaagccac tatttcttac attagagaag aaagctgcag atgcaggggt
     1441 gagttatgtt gttgctttac tttttagctt gcttgctgga actacattag gtatttgggg
     1501 tattgctatt gttacaggaa ttctatgctc ctatattgat aagaataaac ttaatactat
     1561 aaatgaggtg ttagggattt aattattctt tattgatata aaaagtccta gcaatccaaa
     1621 tgggattgct aggaccaaac aaagtagatt atatagcata aataggttta attttgctac
     1681 gggggcgtta tttaggtttt ttcttctttc gaaaaaatct ttctttatga agttaaaagc
     1741 tatgtattca atagcatatt ttgaatatgg acatagaata gtgcttatca ctattgcata
     1801 tagcatctta tctgacacaa ggaaataata cccttcgctg ttttttgtta taaggtatat
     1861 atatataagt gtgcagtaca ggccaaataa aatatttttt atgtagtatc ttaagctcat
     1921 aaattaaacc tcgccatata ttcttttcat tttataagga tcgagttatg aggaaaagat
     1981 tttttgtggg aatattcgcg ataaacctcc ttgttggatg tcaggctaac tatatacgtg
     2041 atgttcaggg agggaccatc gcaccatcct cctcttctaa actgacgggg atcgcggttc
     2101 agtagaaaag attaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2161 gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg ccggatcaag agctaccaac
     2221 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2281 gtagccgtag tcgggccact acttcaagaa ctctgtagca ccgtttgtgc catcatcgct
     2341 ctgctaatcc ggttaccagt ggctgctgcc agtggcgtta aggcgtgcct taccgggttg
     2401 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2461 acacagccca gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta
     2521 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2641 cctgtcgggt ttcgccacct ctgacttgag cgtctatttt tgtgatgctc gtcagggggg
     2701 cggagcctat ggaaaaacgc ctgctacgtg gccttcttcc tgttcctggt cttttgctca
     2761 catgttcttt ccggccttat cccctgattc tgtggataac tgtgttaccg tttttgtgtg
     2821 agtcagtacc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     2881 ggaaaagcgc ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggc
     2941 atggcgtact tttcatacaa tccgcactga tgccgcatgg ttaagccagt atacactccg
     3001 ctatcgctac gtgactgggt cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg
     3061 ccctgacggg cttgtcagct cccggcatcc gctcacagac aagctgtgac cgtctccggg
     3121 agctgcatgt gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa
     3181 agctcatcgg cgtggtcgtg aagcgattca caaatatcgg cctgttcatc tgcgtccagt
     3241 tcgttgagct tctccagcag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     3301 gcggtttttt cctgtttagt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     3361 taatgatacc gatgaaacgc gagaggatgc tcacaatacg ggttactgat gatgaacatg
     3421 cccggttact tgaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc
     3481 tgggggagcc ggttgcccgt tccggaaaac tgccgacact ggcaccgccg ttactgcgtc
     3541 agctggccgc catcggaaat aacctgaatc agacagcccg taaggtgaac agcgggcagt
     3601 ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat ggccatcggg gatgagctgc
     3661 gccggctgcg tctggctgtc agggaacagg gggcgcggga tgatagttaa atttcatgcc
     3721 aggggaaaag gtggtggcag tggtccggtt gattacctgc tggggaggga gcgtaaccgc
     3781 gaaggcgcaa cggtgcttca gggtaatccg gaagaagtcc gggaactcat cgatgccacg
     3841 ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg
     3901 ggaggacgtg aaaaagtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa
     3961 aatcagtaca gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt
     4021 gtcattccga acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgcgca
     4081 gacaggccta gaattgatgc ctggcagacg ctggtaaatc accattacgg gctgcatgac
     4141 ccgaacgccc cggagaaccg caggacgctg acactccctg ataacctgcc tgaaacgaaa
     4201 caggcgcttg ctgagggcgt cacgcgaggt atagatgcac tttaccatgc cggagagata
     4261 aaaggccgtc aggatgtgat tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg
     4321 acgcgaagca gtatcagcat tgcagatccg aacggcggga agaatatcag gctgaaagga
     4381 gcattttatg agcaatcttt tgcagacggg cgcggagttc gagaaaaagc tgaaagagag
     4441 agccgaatct acagagaaaa tgctgaacaa cgagttcagg aggctcggcg aatctgtaag
     4501 cgaggctgtg acatcaaacg agacgaaaat cagagacgct atagccctgt tcacagcctc
     4561 gacagaggaa tcgctggaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
     4621 gagggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tagtctttct
     4681 cctgtttacc gcgagtggcg ggacgctctg gtatcttgga gggaggatac aggcgaacct
     4741 ggaagaaatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt
     4801 ggagtttgtg caggacggga acaggaaatt ccttgtcctt ccgtacggga aatcagcgga
     4861 ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca gagtgacaga
     4921 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
     4981 gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgctta cgcaacggga
     5041 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga
     5101 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
     5161 atacaggagc gcgaacatgt gttatccctg gaacgggagc gtcagccgga aatacaggaa
     5221 cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
     5281 attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa
     5341 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     5401 tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc
     5461 cacttactgt ctgccctcgt agccatcgag ataaaccgca gaaatcgtgt cagccagcag
     5521 cctggatttt ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt
     5581 gccccggagt gagtttaaaa attcctggct gtgaaactgc tgaatgcgtt gttgcacatc
     5641 actccgtgat tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa
     5701 aggcaccatc attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc
     5761 atcagaagca tatacgtaat gccgggtatg ttgttccacg ctgccctttg gggtacaggc
     5821 tgtgagagct actgttgctg ttatcatggc gataagacaa agtttgtttc cggtacgtct
     5881 catgagtgtc tccctacctt aaagtattta ttctcggtga gttattatcc gaagactatt
     5941 ttatcatcag tctctgacag cgttcagcga ccgaacaacc tggaccagaa tccccgtttc
     6001 ttttccagtt ccagtgcctg cctgagctgg gctatctcat tttgtagctg ttcccgttct
     6061 gcttcctggc gtctgcgatc catatcctgt gcctgtttat cctcaagcat cagcgtcagg
     6121 cattgtttca gctcattcag ttcccggaga atgcgttctg tctgctgatc atgtggattt
     6181 tctgcatgtc cctcactgtg cctttctggt gtctcattct gctttaattc gccgtatgcc
     6241 cggatcagtt cactggtttc aaactcccgt cgtccgtccc tgccggtacg gtaactgaca
     6301 aggcctgcgc gcatatcacg gtaaaactgt gaacgcgatc tgcctgtcat ttttagtgcg
     6361 tcccggagtg tgtgccatgc cataaagtga cagtgtccca tagatgtctc atctcatagt
     6421 ttcagtaaaa cataatgagg tctgagaacg gtaatgtttg tgctggtttt tgtggcatcg
     6481 ggcgagaata gcgcgtggtg tgaaagactg tttttttgat cgttttcaca aaaatggaag
     6541 tccacagtct tgacagggaa aatgcagcgg cgtagctttt atgctgtata taaaaccagt
     6601 ggttatatgt acagtattta tttttaactt attgttttaa aagtcaa
//
