LOCUS       NZ_CP116080             7939 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Escherichia coli strain DETEC-S586 plasmid pDETEC51, complete
            sequence.
ACCESSION   NZ_CP116080
VERSION     NZ_CP116080.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31988516
            Assembly: GCF_027944615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Extended-spectrum beta-lactamase genes traverse the Escherichia coli
            populations of ICU patients, staff and environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2023) Institute of Microbiology and Infection,
            University of Birmingham, Institute of Microbiology and Infection,
            Birmingham B15 2TT, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 01:50:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,261
            CDSs (total)                      :: 5,142
            Genes (coding)                    :: 4,937
            CDSs (with protein)               :: 4,937
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 65 of 205
            Pseudo Genes (incomplete)         :: 145 of 205
            Pseudo Genes (internal stop)      :: 38 of 205
            Pseudo Genes (multiple problems)  :: 43 of 205
            Pseudo Genes (short protein)      :: 5 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116080.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DETEC-S586"
                     /isolation_source="ICU staff rectal swab"
                     /db_xref="taxon:562"
                     /plasmid="pDETEC51"
                     /geo_loc_name="China: Guangzhou"
                     /collection_date="2019-09-01"
     gene            complement(126..284)
                     /locus_tag="PIC49_RS26325"
     CDS             complement(126..284)
                     /locus_tag="PIC49_RS26325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(943..1134)
                     /locus_tag="PIC49_RS26090"
                     /old_locus_tag="PIC49_26095"
     CDS             complement(943..1134)
                     /locus_tag="PIC49_RS26090"
                     /old_locus_tag="PIC49_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1199..1522
                     /locus_tag="PIC49_RS26095"
                     /old_locus_tag="PIC49_26100"
     CDS             1199..1522
                     /locus_tag="PIC49_RS26095"
                     /old_locus_tag="PIC49_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1731..>2162
                     /locus_tag="PIC49_RS26100"
                     /old_locus_tag="PIC49_26105"
                     /pseudo=""
     CDS             1731..>2162
                     /locus_tag="PIC49_RS26100"
                     /old_locus_tag="PIC49_26105"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2200..2718
                     /locus_tag="PIC49_RS26105"
                     /old_locus_tag="PIC49_26110"
     CDS             2200..2718
                     /locus_tag="PIC49_RS26105"
                     /old_locus_tag="PIC49_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            2725..2958
                     /locus_tag="PIC49_RS26110"
                     /old_locus_tag="PIC49_26115"
     CDS             2725..2958
                     /locus_tag="PIC49_RS26110"
                     /old_locus_tag="PIC49_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3669..3908
                     /locus_tag="PIC49_RS26115"
                     /old_locus_tag="PIC49_26120"
     CDS             3669..3908
                     /locus_tag="PIC49_RS26115"
                     /old_locus_tag="PIC49_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            3905..4267
                     /locus_tag="PIC49_RS26120"
                     /old_locus_tag="PIC49_26125"
     CDS             3905..4267
                     /locus_tag="PIC49_RS26120"
                     /old_locus_tag="PIC49_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(4292..5185)
                     /locus_tag="PIC49_RS26125"
                     /old_locus_tag="PIC49_26130"
     CDS             complement(4292..5185)
                     /locus_tag="PIC49_RS26125"
                     /old_locus_tag="PIC49_26130"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(5188..6780)
                     /locus_tag="PIC49_RS26130"
                     /old_locus_tag="PIC49_26135"
     CDS             complement(5188..6780)
                     /locus_tag="PIC49_RS26130"
                     /old_locus_tag="PIC49_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(6819..7517)
                     /locus_tag="PIC49_RS26135"
                     /old_locus_tag="PIC49_26140"
     CDS             complement(6819..7517)
                     /locus_tag="PIC49_RS26135"
                     /old_locus_tag="PIC49_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000939065.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     Typing          788..867
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(213..755)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1167..1516
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          1513..3062
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaaacgaaa aaaccgcctg gggaggcggt ttagtcgaag gttaagtcag ttggggactg
       61 cttaaccggg taactggctt ggtggagcac agataccaaa tactgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 gtggctgctg ccagtggcgc tttgtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgagcc gagataccta cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttatgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccaacggcg cagccttttc ctggttctcg ttttttgctc acatgtttct tttggcgtta
      601 tcccctgatt ctgtggataa ccgcatctcc gcttttgagt gagcagacac cgctcgccgc
      661 agccgaacga ccgagtgtag cgagtcagtg agcgaggaag cggaagagcg ccggaacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacatcgga catggtgcgc tttccataca
      781 attcgtactg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggt
      841 tcagggcttc gccccgaaac cccctgacgc gccctgaggg gcttgtctgc tcccggcatc
      901 cgctcacaga caagctgtta ccgtctccgg gagctgtatg tgtcagaggt tttcaccgtc
      961 atccccgaag cgtgcgaggc agctgcggta aagctcgtcg gcgtggtcgt gaagtgattc
     1021 acaaatatcg gcctgttcat ctgcgtccag ttcgttgagt ttctccagca gcgttaatgt
     1081 ctggcttctg ataaaacggg ccatgttaag ggcggttttt tcctgtttag tcactgatgc
     1141 ctccgtgtaa gggggatttc tgttttatgg ggtaatgata ccgatgaaac gcgagaggat
     1201 gctcaccata cgggttactg atgatgaaca tgcccggtta ctggaacgct gtgagggtaa
     1261 acagctggcg gtatggatgc ggcgggtctg cctgggtgag ccggttgcac gctccgggaa
     1321 gctgccgaca ctggcaccgc cgttactgcg tcagctggcc gccatcggga ataacctgaa
     1381 tcagacagcg aggaaggtga acagcgggca gtggtcttcc ggtgatcggg ttcaggtgtt
     1441 ggctgcgctg atggccatcg gggaggagct gcgtcggctg cgtctggccg tcagggaaca
     1501 gggcgcgcgg gatgattgtt aaatttcatg ccaggggaaa aggcggtggc agtggtccgg
     1561 ttgattacct gctgggaagg gagcgaaacc gggaaggggc aacggtgctt cagggtaatc
     1621 cggaagaagt ccgggaactc atcgatgcca cgccatttgc gaagaaatac acgtcaggtg
     1681 ttctgtcgtt cgcggagaag gagctgccgc cgggaggacg tgaaaaggtg atggcgagct
     1741 ttgagcgtgt actgatgccc ggtctcgaaa agaatcagta cagcatcctg tgggtggagc
     1801 accaggacaa gggacggctt gagctgaatt ttgtcattcc gaacatggag ctacagaccg
     1861 gaaaacgcct ccagccgtac tacgaccggg cagacaggcc gagaattgat gcctggcaga
     1921 cgctggtgaa tcaccattac gggctgcatg accccaacgc cccggagaac cgccggatac
     1981 tgactctccc tgataacctg ccggaaacga aacaggcgct tgcagagagc gtcacacgcg
     2041 ggatagacgc tctttatcat gtcggggaga taaaaggtcg tcaggatgtg attcaggcgc
     2101 tcacagaggc agggcttgaa gtggtcaggg tgatgcgaag cagtatcagc attgcagacc
     2161 cgaacggtgg gaagaatatc aggctgaaag gagcatttta tgagcaatct tttacagacg
     2221 ggcgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagac aatgctgaac
     2281 gacgagttca gcaagctcga aaaatctgta agcaaggctg tgacatcaaa cgagacgaaa
     2341 atcaaagacg ctatagccct gttcacagct tcgacagagg aatcactgaa aaaacaccgg
     2401 gaaggggtga aagaggcgat gatgcagcac aggaaggacg tgttaaagct ggcagggaat
     2461 acgggcatga tgttactggg gatggtcctt ttcctgttta ccgtgagtgg cgggacgctc
     2521 tggtatcttg gcgggatgat acaggcgaac ctggaagaaa tcaggataca ggaagaaaca
     2581 ttgcagaaac tgaacgcgaa gacatggggc gtggagtttg tgcaggacgg gagaaggaaa
     2641 tttctggtta tccctcaggg gaaatcggca acggtgattc cctatcaggg gaaggactgg
     2701 gtacaactga cggagtgaca cagagtgacg gagctggaaa gacatttgct gaacgcctta
     2761 gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa
     2821 ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg
     2881 cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag ccagttatac
     2941 agcgagaaca ggagataagg gatgaacggc tgatacagga acgcgaacat gagttatccc
     3001 tggagcgtga gcgccagttg gaaatacagg aacggacaca ggatggccct tcgctgggat
     3061 ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat
     3121 ggttggttcg ggggcaccgg gaacgcgctt cagtcagacc ttgctgaaaa cacaggaacg
     3181 cgttaaaacg catacgggcg cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat
     3241 tgccagatcg cgctcattcc cctgaacttc tgttttcccg ctccggtccc cgtcttcttc
     3301 gcttccgaaa gcatgaaaaa aatccgtttc agctgtctct ggctggcaat tcctgcgtga
     3361 ttcacatggc tgcatagcta tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt
     3421 tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt
     3481 cagcggtgcg tacaataagg gattatggta aattatcttg ttccttagag aatgatttga
     3541 ggtggtatcc tgcacttatt taaatatgct tgcttgggaa attccaggtt gcagtatggt
     3601 tgagttgtaa ttttcctatg ataggaaaaa tgttatatta ttaatttatt tgtgaggctt
     3661 cataaagaat gaaaatttct gatattgaaa aaaacatata taaaggacta gctgaattta
     3721 tttcacaaaa aatcagagat gaacatgata tatgccttgg acagtttgaa tcagaggaat
     3781 tacttgatga gattcttaaa agagtaatcc cagtcatata taatgtggct attgatgatg
     3841 ctataaaatc agtaaggaat gttggggata gattagagga ggaattagat atgaggaagg
     3901 tagtgtgact aattttaaat cagacttaaa tctaataaga aaagaatggt ataatgcatt
     3961 ttattcaggt aatactgaaa tgctagatta cttggaagct gattggttta tttcaacaaa
     4021 tggtcaaaaa gttatctata aaaaacatca gttacgaaag attgatatgc gtccagcgag
     4081 gattttacct agcatagaaa gatatgaata tgatgtcgta atgcgtgagt ttaaaggcat
     4141 tgcttgtgtt tcagggaaag ctgaaataaa gcaaaatgat ggaaatatat taatggcttt
     4201 tattgagaat tggataaagg ttaatggtgg atggaaaatg cagtttatat cgtatgaaaa
     4261 aatctaaggg gaagccccct tagattaatg gttatatatg ttcaagtttt atttttttat
     4321 agcgataagt tctgctttga gctttaagca tgttttctgc aaactgaata tcaaaactat
     4381 ttatagcatt agttattata gtttttattt tttctttatc agataatgaa tcaccatgct
     4441 ctataggttc ccgcatatat ggattgtttt ttaattcatc aaataatgtt ttaaatagtt
     4501 ctaatcgacg aggtcttata actgtaaaag atatatgaag agagtgtttg ctctgagtcg
     4561 taacattgtg ccagtatcca gcaggaagat atatggcctg acctggggtt atatcaaact
     4621 cataatctgg ggaggtatta ggtggtagat aatcgaagct tctttcatct tcgagagggt
     4681 atgaataggt agggttgtaa actttccatt tttttattcc ctcaatttga acagctatta
     4741 catcatgatt gtcaaaatga agcccaaagc ttggtgttgc tgtaaatgca gcatatagat
     4801 tagcactaca tgtacattct aactctttag ataaccatag tctgctttca tctacagatt
     4861 caaagaatga ctggcatcga tctactataa gggttgcgcc atcacgtaag catttatata
     4921 gctgatggcg atttatatgt ggtgttctat cgcctgtttg gctatatgtg tatcttataa
     4981 atcctttata ccccctaatt tcgggaaagt tgtcatttgc cattctaact ctaggatagt
     5041 gcaatatatc tttttcaagc aatttattaa gatcattcca gttgattaag tttttataat
     5101 taacatccgt gaatacaaac ggatatttct ggaaattata tttcttaaat ttttctatga
     5161 tttccttttt tataagatta ttcattttta aacctcatgt tttgtgatat ctataatctg
     5221 tgctttaggt atattattta taagttgttc atcatgagat ataacaatga tatgcttttc
     5281 cttgttttcc tcttttatat aatcactgat tatttttata ccaagtgaat caaacccttt
     5341 tgtcggttca tcgaaaataa tcaagtctac gttttttcca gatgtaagag atactcctaa
     5401 tttttgtttt tgtccaccag ataaattatg tccgtaacct ttgatctcat aatctagtgg
     5461 tttgtcgaac ttaaatctgt gtagcagctt tttaattgta tttaaattat taatgccgga
     5521 ataatgtaat atattttcct ctatagttcc aggcatgaga tctagatctt gaggaacgta
     5581 taagacatta acaaaaggag aatttgcttt ttcttgttgt atcccattga tttttattgt
     5641 gttttcttct gttgttatat tgccagagag aatttttgca atagttgttt ttcccgaacc
     5701 attctcaccg gttataaagg taacaacgcc ttttgttaat attaatttat ggttgaatga
     5761 tttgttgtat atttcaatat tgcaaatatt gtcttttaca tgaagatatt gcttgtctgt
     5821 gtaatttgtt ttttttatgt cgtttatttc ggcacagtat aaatcaattt ctgttcttgc
     5881 catttttatt tgtctaaata gataaccaat gcgatttaat gggagaaaaa cttggaataa
     5941 aaaagtggca agcataataa tactaccacg gcttgctcta ccatgaagaa acatccagta
     6001 tgctgatagg ataatcagac tactaaaaat gactgaaaga aaagcggagc tgataccaaa
     6061 caggaaaaat tcattattta cttttctttg tttttctgaa tgtgtattta cagcttgaat
     6121 tactcgttta cgacttttat ccgtggtatg gaaagcttta tccataggat acatgtgcat
     6181 catatcaaac attcttccag atgcgaattt ggagctaagt gcaactgatt tcatcattgg
     6241 aaatctacgt tgtgtcattt ttatgacaaa tattatatag attgccatta ttattataaa
     6301 ctcaatggct attaatgtat taacacttat acaaattgtt atgattattc caatcatttg
     6361 tattaataat ggtactacat cagaacatat tgtataaaat aatgatgatg cagaagaaag
     6421 catgctttct ataaccttat ttagttctcc tttatttttc tttaaaaaat gctcttggtg
     6481 agaattttgt atattatcaa taacttgctc tctaagcttt acagataatt ctatatttgt
     6541 tttttgagtc aagtattccg atatgctata tccagcaggc ataaggaatc taattgataa
     6601 gaaacatgct ataaatatta ataatgagtt tatttctcct attgaatctt tattgtcaat
     6661 aactgttttt attaaatatc cgaatagaac gaaaagaaaa ctacttatag ttgataataa
     6721 tataataaaa aacaaattac atttattaaa tgtgatgatg tacttttcaa taaaattcaa
     6781 tttataatcc ttaaagcccg tatattacgg gctttatgtt atctagaatt acatgagcac
     6841 ccagcgccac atcctttacc gctgcaacca caaacagaac cattatcatt ccaaacatct
     6901 tttcgcatta ttatttttac aaattctgcg gctggaaggt gagtgatact ctttactttt
     6961 tcgatgtaag aatcactgga tatggattct tttggaattc ttatagtatt aatgttcttt
     7021 atgctgtatc ttacagcata atcaccatac cactttgcga cagtttcatg acctaggtaa
     7081 aaagcgattt tttcaatttg atttttatat tcgttaaaac gatattcttg gaatgttata
     7141 ccatgctcat taacatgcct tatctcattt tttagcatgt catttgtatt tggataatgg
     7201 tggaggtaag caccaagata tttttcacaa aagctggtat aagcatctgt aaattgaata
     7261 aacgtatgcc acatttcatc tataatttgt aattgctcgt gaacttttat attgtagcat
     7321 tccgttaggc atgttgaggc tagccagatg aatttctttg tttcattaaa gatatcatca
     7381 gcttcttctg gtgttacatt ccattcttta cgaaatttta gaagaacatc atcattgtga
     7441 taactcatga tatcttctaa tgagctaaca ttatgaactt ttgcataaga actgctgttc
     7501 atcttatgtt gtagcataac acccccaatt atatatttga atgtagatga gaatagttat
     7561 catttgttat ggtattgtta aatttgtgct tgtgtcaata tgcctaattc tataaatgag
     7621 atagtaatca agttttttaa tggttatagg atccattagt aatacaaggt ttttgcagcc
     7681 agtttttacg ggtaggggct gcaaggaaaa caaaattttc ccactctaag agaagagaat
     7741 tgagttggtt gaatgagtgg atgcatctag ggccgctaat tcaccgagtg taaaggctgt
     7801 acgaaatgtg ttgcagtccg ataatagccc cctccattga cgttttacac atgtgttgta
     7861 ggttccagga gtgggcggat ctcaaaaaga tcaaaggatc ttcttgagat cctttttttc
     7921 tgcgcgtaat ctgctgcca
//
