LOCUS       NZ_CP115932            13823 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain 43UC01 plasmid
            pKpUC43_14k, complete sequence.
ACCESSION   NZ_CP115932
VERSION     NZ_CP115932.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07766632
            Assembly: GCF_027941975.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   Nguyen,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) JCVI, 9704 Medical Center Drive, Rockville,
            MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.7
            Expected Final Version            :: yes
            Genome Coverage                   :: 30xx
            Sequencing Technology             :: Minion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:38:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,660
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,354
            CDSs (with protein)               :: 5,354
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 113 of 184
            Pseudo Genes (incomplete)         :: 78 of 184
            Pseudo Genes (internal stop)      :: 23 of 184
            Pseudo Genes (multiple problems)  :: 25 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115932.1.
            Bacteria and source DNA available from J Craig Venter Institude.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="43UC01"
                     /isolation_source="indwelling urinary catheter"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pKpUC43_14k"
                     /geo_loc_name="USA: Lubbock, TX"
                     /lat_lon="33.58 N 101.86 W"
                     /collection_date="2013-08-13"
                     /collected_by="Dr Randall Wolcott"
     gene            1346..3310
                     /locus_tag="DLD89_RS27000"
                     /old_locus_tag="DLD89_26995"
     CDS             1346..3310
                     /locus_tag="DLD89_RS27000"
                     /old_locus_tag="DLD89_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3310..4041
                     /locus_tag="DLD89_RS27005"
                     /old_locus_tag="DLD89_27000"
     CDS             3310..4041
                     /locus_tag="DLD89_RS27005"
                     /old_locus_tag="DLD89_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4048..4578
                     /locus_tag="DLD89_RS27010"
                     /old_locus_tag="DLD89_27005"
     CDS             4048..4578
                     /locus_tag="DLD89_RS27010"
                     /old_locus_tag="DLD89_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4606..4785)
                     /locus_tag="DLD89_RS27015"
                     /old_locus_tag="DLD89_27010"
     CDS             complement(4606..4785)
                     /locus_tag="DLD89_RS27015"
                     /old_locus_tag="DLD89_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4854..5231
                     /locus_tag="DLD89_RS27020"
                     /old_locus_tag="DLD89_27015"
     CDS             4854..5231
                     /locus_tag="DLD89_RS27020"
                     /old_locus_tag="DLD89_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5293..5592)
                     /locus_tag="DLD89_RS27025"
                     /old_locus_tag="DLD89_27020"
     CDS             complement(5293..5592)
                     /locus_tag="DLD89_RS27025"
                     /old_locus_tag="DLD89_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5582..5845)
                     /locus_tag="DLD89_RS27030"
                     /old_locus_tag="DLD89_27025"
     CDS             complement(5582..5845)
                     /locus_tag="DLD89_RS27030"
                     /old_locus_tag="DLD89_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6151..>6441)
                     /locus_tag="DLD89_RS27035"
                     /old_locus_tag="DLD89_27030"
                     /pseudo=""
     CDS             complement(6151..>6441)
                     /locus_tag="DLD89_RS27035"
                     /old_locus_tag="DLD89_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(<6509..9482)
                     /locus_tag="DLD89_RS27040"
                     /old_locus_tag="DLD89_27035"
                     /pseudo=""
     CDS             complement(<6509..9482)
                     /locus_tag="DLD89_RS27040"
                     /old_locus_tag="DLD89_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            9644..10201
                     /locus_tag="DLD89_RS27045"
                     /old_locus_tag="DLD89_27040"
     CDS             9644..10201
                     /locus_tag="DLD89_RS27045"
                     /old_locus_tag="DLD89_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            10435..10988
                     /gene="aac(6')-Ib"
                     /locus_tag="DLD89_RS27050"
                     /old_locus_tag="DLD89_27045"
                     /pseudo=""
     CDS             10435..10988
                     /gene="aac(6')-Ib"
                     /locus_tag="DLD89_RS27050"
                     /old_locus_tag="DLD89_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /name="aac(6')-Ib"
     gene            11058..>11273
                     /locus_tag="DLD89_RS27055"
                     /old_locus_tag="DLD89_27050"
                     /pseudo=""
     CDS             11058..>11273
                     /locus_tag="DLD89_RS27055"
                     /old_locus_tag="DLD89_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            11339..12041
                     /locus_tag="DLD89_RS27060"
                     /old_locus_tag="DLD89_27055"
                     /pseudo=""
     CDS             11339..12041
                     /locus_tag="DLD89_RS27060"
                     /old_locus_tag="DLD89_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            <12095..12265
                     /locus_tag="DLD89_RS27065"
                     /old_locus_tag="DLD89_27060"
                     /pseudo=""
     CDS             <12095..12265
                     /locus_tag="DLD89_RS27065"
                     /old_locus_tag="DLD89_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            <12414..13216
                     /locus_tag="DLD89_RS27070"
                     /old_locus_tag="DLD89_27065"
                     /pseudo=""
     CDS             <12414..13216
                     /locus_tag="DLD89_RS27070"
                     /old_locus_tag="DLD89_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            13206..13463
                     /locus_tag="DLD89_RS27075"
                     /old_locus_tag="DLD89_27070"
     CDS             13206..13463
                     /locus_tag="DLD89_RS27075"
                     /old_locus_tag="DLD89_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            13578..13715
                     /locus_tag="DLD89_RS27080"
                     /old_locus_tag="DLD89_27075"
     CDS             13578..13715
                     /locus_tag="DLD89_RS27080"
                     /old_locus_tag="DLD89_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_271259632.1"
                     /translation="MDFSGFLLVACQANYIRDVQGGTVAPSSSSELTGSRFSRKIKGSS
                     "
                     /name="colicin release lysis protein"
     ARG             11071..11327
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             10385..10731
                     /name="AAC(6')-Ib7"
                     /gene="AAC(6')-Ib7"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.04"
                     /coverage="44.06"
     Typing          complement(2..547)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.082"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3311..4038
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac
       61 ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg
      121 ctgctgccag tggcgttttg cgtgcttttc cgggttggac tcaagtgaac agttaccgga
      181 taaggcgcag cagtcgggct gaacgggggg ttcttgctta cagcccagct tggagcgaac
      241 gacctacacc gagccgagat accagagtgt gagctatgag aaagcgccac acttcccgca
      301 agggagaaag gcggaacagg tatccgggaa acggcagggt tggaacagga gagcgcaaga
      361 gggagcgatt catcggaaac ggtgatgatc tttaagtcct gtcgggtttc gccactcctg
      421 actgattcat ggttgagcca ccggctccca cagatgcacc gaaaaagcgt ctgtttatgt
      481 gaactcagtc aggagggcgg agcctatgga aaaacgccac ctgcgtggcc ttgccgtttt
      541 gcctcacatg ttagtcccct gcttattcac ggaatctgtg ggtaactttg catgtgtccg
      601 cagcgcccgc cgcagtctca cggccggagc gtagcgaccg agtgagcgag gaggcgcaat
      661 ttccccggca cctacaagac ggacaccgga cgcgctgatg tggcatattg tcccctgatt
      721 aagtctttat attccctgat aataaattac caggataggt gaatcatatc ggtttttcgc
      781 tgaacatttt acgttaacct atctctgttg cttttttctc cttacggaga aatacaggtc
      841 atccccgcga gggcgtggct gacctcaggg ggatatcccc tgcttacgct ggctggacgc
      901 caccgacgca cggtctctcc ccctctttct ggctccgtcc ggctggacgc cggccacgcc
      961 agaaattcac ccggcccccg tcaggctcac gcctgaccac ccacaaaaat ccacggtgct
     1021 ggacgcacct ggcgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
     1081 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
     1141 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
     1201 cggaaagagg ctggcctccg tggtgaaagc tgaacgcaat caccacggac gtgaccgccg
     1261 ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg ggctgcacat cacgccggca
     1321 aaccatcgtc tgaaggagag agaacatgtt taatacggat tcgcttgcct ggaagtggca
     1381 cattccggag ctggcccggc agatttatcc gtgggtcagc tggctgtacc aggagtatta
     1441 cggtctggcc ggggctttcc tagttatcgg cctgtttttc ggtctggctg aactttgggc
     1501 cggacggatg cgttttgtgg ggctgagtca taaggagcga cgggagtatg ccgatcgcta
     1561 taagcgcgat aataaacggc cagcgccatt ttcggtgctg gcctcgctgg attcgcccgg
     1621 gcggatggtc agggcacgtc gacatgtctg gaactacgcg tactggctga ccggcgcggc
     1681 cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct ggggagaaac
     1741 cgtcctgcgt ccggacctct acgcaaacgc agattattac ctgctgcagc cggctaacct
     1801 gttcgggatt gcctggtgcg cgggcgggct ggctgtcggg attgtgttgg cgcggctgct
     1861 ggcgttcctg ctgcttgacg gtcattttgc ggctgctgac gaggcggtga acgcgaaact
     1921 gaatcaggag agccggcgca gcagccagcg gacaggagag atgaccgacg tgcgccacct
     1981 gcatttcggg gagccggtac cggttaatgc gctggccgat ttttcctcgg agcaggcccg
     2041 gcaggagcag gcggtgtttc tgggtaagga tgagcagggc aagccggtgc tggttccgcg
     2101 tgatacctgg cgcaagacca acattcagat cctcggcctg cccgggagcg gcaagagcgt
     2161 gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgcgg tggtgtactt
     2221 cgaccccaaa ggggacgcat gggcgccgca cgtcttccgg gcgcactgtc cggacttcac
     2281 cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca
     2341 gtacgcgctg aaaaacctgc tgattgccgg gcttaacctg ggcgagaccg gcgacgtggc
     2401 ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agtttccgca
     2461 gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ctgccggagg ccctgaaaaa
     2521 ggacgtgaag gggttgatca ctaggctgga aaacgtcgct gacctgagcg tcctgcagac
     2581 ggacagtggc atcgatgtgg cggggattat caacggcggc ggctgcctgt acgttatcgg
     2641 ctcgatggac gacgaggcgg tgatccgtgt gcaaaaaatg ctgtttgccc gctgcgcgca
     2701 aatcatcatt gcgcgggacg agttccggga gtggccacac gtcagcgtga tgctcgatga
     2761 aattaaatat ctgctgtcga agtatgtact gaacgcactg gggacgctgc gcagccggga
     2821 ctgcaatctg ttgctcgcgc accagtcgct gggcgatttc gggcagtgcg ggcaggatct
     2881 gcccgctgaa tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttttaccg
     2941 ggcggccagc ctggagtccg cgcagtgggc ggcggggcag accggggaaa tcagggtgga
     3001 cgtggagcgc cgccgtgcca gccgggaggc ggggaacgtg gagcatatca gcggggacag
     3061 cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgccgga
     3121 cgggttcgcg gtcatgaccg ggctgggtgt ggcccggatg gcgttcagca gtccgctgcg
     3181 ggttgagcgc cgggaaatcc cgttgcagcg tttcccggtg ctggcgaaaa ccgatcccct
     3241 ggcggagttc cggcccccgg ccgacggcgg cgggccgaaa gcggatgatg attttgcggg
     3301 gatttactga tggcgctgga gctgtacaac gtcagccacg cgaaacgaca ggcgcggaac
     3361 gcggagaaaa cccggctgac cctgcgctgg ttgcgggaag agctgtgttc cacggcggag
     3421 ctggttgcgc ggcggctggg gattgcggcg gtgcagccgg tctaccgctt cctcgacagc
     3481 ctggtggcga aagggctgct ggtccggacc aaatatccgg tggacggccg tcaggtcagc
     3541 gtgtgggggc tgacgccgca tggcgtggcg ttctcgttcg acgaggacga accgctggcg
     3601 gatgttattc cgtttcagcc gtcaagggtg tcggcagcac atctgccgca ccggctggcc
     3661 gtgcaatccc tgcgccttga tatggaagcc agaggggcca ccggctggcg cattctgcac
     3721 cgtctgagtc ttaaaggcat gaaagtgccg gacgccctgg cggagctgga cggtaaaacg
     3781 gtggcgtttg aggtggagcg caccgtcaag agccgtcgcc ggtatcagga agtggtgtcc
     3841 gggtatctgt tcaaccgcaa agctaacggc atcgatgaaa tctggtacat ctgcccggac
     3901 cgggcgacgc aggtcagggt gcagcgggca atcctgtcgg tggatgaaat cgtgaaccca
     3961 cagaccggcg aggcccgcaa aacggcagag ctggatcgtg aacggttgtt tgcgtgcttt
     4021 aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat tgaagacgcg
     4081 gaggaccgga acggcgagcg ctgcatcatg ccgaccagcg agcatctgga agatatggtg
     4141 gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg
     4201 gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg
     4261 tactttttca ctggtaaaaa tccgcaatgg gaacggaaca aacccacagg aatgaagctg
     4321 tgtgattacc tctatcgcaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt
     4381 gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgacgtggt gagtgagcgg
     4441 cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc
     4501 cgctatttta acgtgccggt acctgaactg ccggaggaca aacctgcccg ccggtggtgg
     4561 ccgttcggca aaaaataaat gccagcaccg gccatgccgg tgctgttatt cctccccgag
     4621 cttatcccgc atcacctgcc agagtgtttc cgcatcctcg tgtagccgct cgcacatgtc
     4681 ggccggggta tccatatcca gctcattcag tttatcgagc aatagcagcg actgctcctt
     4741 aataaattta gccatattga gggctgtttt ttcctgtttt gtcatgattc tttccttccg
     4801 ggcatggtgt gcgcctgtca ggtatactgt ctcaaaaata gcggggtgag acaatggcgt
     4861 gggtgacggt gagacaggcg acggaactga cgggtaaagc ccgaagttcc ctgtatcggg
     4921 atatggcgaa aggccgcgta tcataccgta cggaagctga tggcggccgg gtggtggata
     4981 ccagcgagct tatccgggtg tacggtgagt taagacagga tgagactcac gagcgggacg
     5041 agttgagact gtcgggtgag actgaaaaaa tggcctccga cgcgttgatc gcggagataa
     5101 aagcactcag ggaggaggtg gccgggctac ggcaggaaat gcagtccatg agactgctgg
     5161 aacacaaacc ggagacagca gagaccccgg ccggaacacc gcgccggtgg tggccgtggg
     5221 gaaaacgctg acgcgctgtg cttgtccaac cggcgacggc cacgcaatga attgcgtccc
     5281 cgtcaccggt tattagagaa caatatgggg attctggcgc tggccgacca cggccagaac
     5341 ccggactttt ctggtctggt catcaacatc atagaggaca tgctgtccca tcagactgat
     5401 ccggcgaacg tcctcgccat agcgtgagtc cacttcatag agtcgcggga agtcggccag
     5461 tttgtccgcc gcagcgagaa cccggtcggt aacgttctgc gcatcccaca gggatgaatt
     5521 ttccgcgata tagtcaaaca gcgtcgttac atcctcgatg gctccctgct tccattccac
     5581 gtcatacggc atcgcgcagt tcctgataac ggccatgcag gcgctggcgg agttgttccg
     5641 tggtcatacc cggacgcgtg tcagcacggg cggcggtcac tttggcgcga acctgatctt
     5701 catactctgc ctgggccagt tcttcgttag ccagtcgctg caacagcgca gctttgtccg
     5761 ggccaccttc cggcagtatg cggggcaggc tttcagccag ttcccgaaga aagttggcgt
     5821 tttcgtagtg tgtagtgttg ctcattgcgg agcctcccgg gttaatgcgg caatatgaag
     5881 gaagtttagc agggggcggt ctgaaagaat atggtcgtgt cagtcaggct gatgaagcag
     5941 aaagtcggta aaaaagggct tacggcatcc attttacgtc aaaacatatg ccatgcagtt
     6001 gcgtgcatag gcatgcatta ggataaaatt tatcgggcgc gtttccggca gttttccggg
     6061 ggggttgttg cctgttttat cccgtagccg ccggaaacgc cctgagcccg tctgagcggt
     6121 gcgcgtaatg acgcgttatg gtaaatccac ttactgtctg ccttcatagc catcgagata
     6181 aacagctgaa atggtgtcag ccagtagcct tgattttttc ggtgtgaggt ctttgctgcg
     6241 gtaatcggta cccgcaaatt gtgttgtgcc ttggagtgag tttaaaaatt cctgactgtg
     6301 aaactgctga atgcgttgct gcacatcact ccgtgatttt ccggtcgctt tatcctttgc
     6361 gcccatatcc cagaactgcc ggaaaaaagg caccatcatt cgtgttgtgt cagctttctg
     6421 ggtggaaaaa ttgggatcaa aaggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6481 caacgttttc tgcctctgac gcctctttta atggtctcag atgacctttg gtcaccagtt
     6541 ctgccagcgt gaaggaataa tggccgagca tattgatatg tccgtggcaa agcggggaga
     6601 ggcgtgcgat atcttcatca ttcagtgttt caccctgcgc ccggagatga tccagagctg
     6661 cctgcatata aatagtgttc cataacacga cggcgttagt gaccagcccc agagctccca
     6721 actgatcttc ctgaccgtcg gtatatcgtt ttcttatctc acctttttga ccgtgacaga
     6781 tggctctggc aactgcatga cggctttctc cccgattaag ctgggtcaga atgcgccggc
     6841 ggtaatcttc atcatcaata taattaagca gatacagcgt tttgttgatg cgccccactt
     6901 caatgattgc ctgagtcagt ccggagggac gttcactttt cagcaatgaa cggaccagca
     6961 ctgaggcctg tactttgccc agcttcaggg agcctgcggt ccggatcatt tcgtcccact
     7021 gaaggactat ttttcgggga tctgattgcc ctctggcaat atcattcagc acgccatagt
     7081 cggcatcatg gtccattcgc cagaaaaccg aagcaccggc atcagccagg cgtggagaaa
     7141 actggtatcc cagcagccag aaaaggccaa agacaagatc gctggcacct gccgtatcgg
     7201 tcataatttc ggttggattc agcccggtct cctgttccag aaggccttcc agcacaaaga
     7261 tagagtccct cagcgtcccc ggtataacga tgccatgaaa gccggaatac tgatcggaca
     7321 caaagttgta ccaggtgatc cctctgttat taccaaagta tttgcggttc ggtccggcat
     7381 tgattgttct gactggcgta acaaagcgca ttccatctgc agatgccact tctcctccac
     7441 cccatatctg tgccagtggc agcgttgcct gaaaatcaac cagtctggca ttagcgctgg
     7501 tgatagtttc agcccgcaga tagttcgctt ttgtccagtt cagccggtgt cgggtcagtg
     7561 caggaacatt tgatctgatc agtggttcca gaccgatatt gcaggcttca gccatcagca
     7621 cggcgctgat gctgacgggc agatcatcaa ctctggcact ggcttcactg gcatggaaaa
     7681 actcatcagc aaatccggta tgggcgttaa tttcgagcag caactccgtt aaatccaccg
     7741 gagggagcag atcactgatc attttgctca gtcgtttcag actgtccggc tcatcaagac
     7801 tggcgagggg agaaattgtc aaccggggct tcgggccaga aacatcgagt tcgacagcct
     7861 cattttcgcc aagacgtgca gcaacctgtc tgtaacgact atcaagctga tggcccagag
     7921 attttattgc ttcctgcggg tctgtcgggt gccccaaaga acgataaacc ttaatccgat
     7981 ttgcctgcca gtcagcaccc tgtagtaatc ttgcacgagg atctccccac cggttactgc
     8041 cggtaacgta gacatccctc cgtctcagac tatcctgcag tttactgaga aagcagagcg
     8101 tgtatcccct tcgggtgata tgtttttcct tgttaatcac cagccgtttc catgactgac
     8161 tgatgatttc cgttggtgcg tcgtcaaaaa actgccgccg tgagctgaac tcccggctga
     8221 ggtagtcaca ggcattcaga gtggtaaccc cggcaggtgc ggatgaaaat ttaacggtat
     8281 tcagcagatg gggcaggaaa cgacgaacgc gcccgtactg ctccaccatt tcgtcatgaa
     8341 aattatcgtc tgagggccgg gcaatttcac ggacaagcgt gatgatttca gccagctttt
     8401 gcctagggat gtagctgaac acctcagcac gaatcgattc gtccggtgtt tcttctttca
     8461 gcaagtacga acatgcgctg gcgagcgcca atgcagattt atccagatcc ttcagcgagc
     8521 ggagccgttt ttttctgccc aatctttctg gcgtcacgga tgataacggc cagcatggcg
     8581 tccagaacgt ccagtgcatc atccagcgcc agcgtttccc atgcaaggac aaaggcaacc
     8641 agaaccgcca tccttttctg cggtgacatc ctggcaatat tgaacaccga agtcatacca
     8701 gcataacgtg cgagattttt caggcgcaca gccgggagtg tactcaggtt ttcagcatgc
     8761 aggccaaaat cgttcagagt tttccagcgt tcaattgctt cattaaacgc cggaccactg
     8821 atggtcacag ggcctttttt tcagtgattc cagtaaagac aggcggctgc aatcagttgg
     8881 ccccagcagc atctccagct gtgaacgctg ttcggctgac ggtatcagtg ccagtttgtt
     8941 ccacaggcgc aacgtcgcct tttcccttac ctctgaaatc aaccgggtca gcgtggtggc
     9001 tccgggagaa taatacgatg ttgcataagc caccctgtcg ccaggtcgaa aagcaggcca
     9061 ggacgttcgt tgcttatcca gctccgggta tataaaagac gggtaaggcg aaatgtccag
     9121 ggccaggcaa attcacgata ctgatagtgc tgacgtatca gcgctgcatg ctcacggcgg
     9181 gtattttccc tctgaccgta ttctgcaaga acggtgatat cacgaatccc gagctgtctg
     9241 gcggtaaaat gccggacgcc ggaaggaata tgattcatgt cagtaagaaa agtgcccaga
     9301 aaacgcacac acccgatttg cagggcaata ccaagtcgat tgtgatcacc ccggcttttc
     9361 ccgataaatt ctttatccgc ttcatcaaga tgaaaataac gcgccagctg aagttcatcg
     9421 ggttcgccag tgaacctgcc ataactctca acctgctcag tggtcaaaaa atcaacgggc
     9481 atatcggcct ccctgcctga cggctttttt aacacaactg caaccgttcg aaatattata
     9541 aattatcaga catagtaaaa cggcttcgtt tgagtgtcca ttaaatcgtc attttggcat
     9601 aatagacaca tcgtgtctga tattcgattt aaggtacatt tttatgcgaa tttttggtta
     9661 tgcgcgggtc tcaaccagcc agcagtccct cgatattcag atcagagcgc tcaaagatgc
     9721 aggggtaaaa gctaaccgca tctttaccga caaggcatcc ggcagttcaa cagatcggga
     9781 agggctggat ttgctgagga tgaaggtgga ggaaggtgat gtcattctgg tgaagaagct
     9841 cgaccgtctt ggccgcgaca ccgccgacat gatccaactg ataaaagagt ttgatgctca
     9901 gggtgtagcg gttcggttta ttgacgacgg gatcagtacc gacggtgata tggggcaaat
     9961 ggtggtcacc atcctgtcgg ctgtggcaca ggctgaacgc cggaggatcc tagagcgcac
    10021 gaatgagggc cgacaggaag caaagctgaa aggaatcaaa tttggccgca ggcgtaccgt
    10081 ggacaggaac gtcgtgctga cgcttcatca gaagggcact ggtgcaacgg aaattgctca
    10141 tcagctcagt attgcccgct ccacggttta taaaattctt gaagacgaaa gggcctcgtg
    10201 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
    10261 acttttcggg gaaaagtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10321 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10381 agtatgagta ttcaacattt ccaaacaaag ttaggcatca caaagtacag catcgtgacc
    10441 aacagcaacg attccgtcac actgcgcctc atgactgagc atgaccttgc gatgctctat
    10501 gagtggctaa atcgatctca tatcgtcgag tggtggggcg gagaagaagc acgcccgaca
    10561 cttgctgacg tacaggaaca gtacttgcca agcgttttag cgcaagagtc cgtcactcca
    10621 tacattgcaa tgctgaatgg agagccgatt gggtatgccc agtcgtacgt tgctcttgga
    10681 agcggggacg gatggtggga agaagaaacc gatcgagagt acgcggaata gaccagttac
    10741 tggcgaatgc atcacaactg ggcaaaggct tgggaaccaa gctggttcga gctctggttg
    10801 agttgctgtt caatgatccc gaggtcacca agatccaaac ggacccgtcg ccgagcaact
    10861 tgcgagcgat ccgatgctac gagaaagcgg ggtttgagag gcaaggtacc gtaaccaccc
    10921 cagatggtcc agccgtgtac atggttcaaa cacgccaggc attcgagcga acacgcagtg
    10981 ttgcctaacc cttccatcga gggggacgtc caagggctgg cgcccttggc cgcccctcat
    11041 gtcaaacgtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
    11101 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
    11161 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
    11221 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacggcac
    11281 tgttgcaaat agtcggtggt gataaactta tcatcccctt ttgctgatgg agctgcacat
    11341 gaacccattc aaaggccggc attttcagcg tgacatcatt ctgtgggcca tacgctggta
    11401 ctgcaaatac ggcatcagtt accgtgagct gcaggagatg ctggctgaac gcggagtgaa
    11461 tgtcgatcac tccacgattt accgctgggt tcagcgttat gcgcctgaaa tggaaaaacg
    11521 gctgcgctgg tactggcgta acccttccga tctttgcccg tggcacatgg atgaaaccta
    11581 cgtgaaggtc aatggccgct gggcgtatct gtaccgggcc gtcgacagcc ggggccgcac
    11641 tgtcgatttt tatctctcct cccgtcgtaa cagcaaagct gcataccggt ttctgggtaa
    11701 aatcctcaac aacgtgaaga agtggcagat cccgcgattc atcaacacgg ataaagcgcc
    11761 cgcctatggt cgcgcgcttg ctctgctcaa acgcgaaggc cggtgcccgt ctgacgttga
    11821 acaccgacag attaagtacc ggaacaacgt gattgaatgc gatcatggca aactgaaacg
    11881 gataatcggc gccacgctgg gatttaaatc catgaagacg gcttacgcca ccatcaaagg
    11941 tattgaggtg atgcgtgcac tacgcaaagg ccggcctcag cattttatta tggtgatccc
    12001 cgggcgaaat gcgcctggta agcagagttt ttgaaatgta aggcctttga ataagacaaa
    12061 ggctgcctca tcgctaactt tgcaacagtg ccctgataaa tctggagccg gtgagcgtgg
    12121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    12181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    12241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    12301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    12361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc cctactccgg
    12421 cacaggtaaa acagcgtctg gaggaagaaa agcgtcgcca gcaggcatgg gacgctgctc
    12481 acccggaaga ggggctgaaa agagtatgat aaagcgaaag ctgagctgga tgctgaagat
    12541 aaaaatattg cgaccttaaa cagtcgcatt gcatcgacag agaaggcgat ccccggtgca
    12601 agggctgctg ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat
    12661 tttgtgactt ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat
    12721 cttgataagg atattcagaa tcagaatgag aaattaaaag ctgcccagac atctttaaac
    12781 gaaatgaatg aatccttatc cggggataag gctgcgcttt cggggcgatg gagagccgga
    12841 aacaaaagga gaaaaagcga aggatgcaga aaataaatta aatgaggaaa agaaaaaacc
    12901 tcgtaaggga actaaagatt acggccatga ttattttcct gatcccaaga ctgaagatat
    12961 taaggggttg ggagagttga aagagggtaa acctaaaacc cctaaacaag gtggtggtgg
    13021 taagcgggcc cgatggtatg gtgataaaaa gcgtaaaatt tatgaatggg attcccagca
    13081 cggtgagctt gaagggtacc gcgccagtga tggcgaacac ctcggggcat tcgatccaaa
    13141 aacgggtaag caggttaaag ggccggatca aaacgaaaca ttaaaaatat ctttaagagg
    13201 taaatatggg gcttaaatta catattcatt ggtttgataa gaaaaccgaa gagtttaaag
    13261 gtggtgaata ctcaaaagac ttcggtgatg atggttctgt cattgaaagt ctggggatgc
    13321 ctttaaagga taatattaat aatggttggt ttgatgttga aaaatcatgg gtttcgatat
    13381 tacagccaca ctttaaaaat gtaatcgata ttagtaaatt tgattacttt gtatcctttg
    13441 tttaccggga tggtaactgg taagcaaaag ttatcaagga tgatttgaaa tacagacttc
    13501 cggtgttcag ggatgaatac cggggtttta aatacaggaa ggtatatgaa aaaggtaaag
    13561 gttgtttttc tttttatatt gatttttcag gctttcttct ggtcgcatgt caggcaaact
    13621 atatccggga tgttcagggt ggaacggtgg caccatcgtc ctcctctgaa ctgacgggat
    13681 cgcggttcag tagaaagatc aaaggatctt cttgagatcc ttttttctgc gcgtaatctt
    13741 tgccctgtaa acgaaaaacc acacgggagg tggtttgatc gaaggttaag tcagttgggg
    13801 aactgcttaa ccgtggtaac tgg
//
