LOCUS       NZ_CP115827            36165 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Escherichia coli strain E1 plasmid pE1-IncFII, complete sequence.
ACCESSION   NZ_CP115827
VERSION     NZ_CP115827.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32542048
            Assembly: GCF_034089005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36165)
  AUTHORS   Dong,N.
  TITLE     Characterization of E. coli strain E1 from bloodstream infection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 36165)
  AUTHORS   Dong,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2023) Department of Medical Microbiology, Soochow
            University, Ren'ai, Suzhou 215123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.5.0
            Assembly Name                     :: E1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 23:26:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,530
            CDSs (total)                      :: 5,407
            Genes (coding)                    :: 5,198
            CDSs (with protein)               :: 5,198
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 76 of 209
            Pseudo Genes (incomplete)         :: 152 of 209
            Pseudo Genes (internal stop)      :: 37 of 209
            Pseudo Genes (multiple problems)  :: 51 of 209
            Pseudo Genes (short protein)      :: 3 of 209
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115827.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..36165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E1"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pE1-IncFII"
                     /geo_loc_name="China"
                     /collection_date="2019"
     gene            1..858
                     /gene="repA"
                     /locus_tag="PBU98_RS27400"
     CDS             1..858
                     /gene="repA"
                     /locus_tag="PBU98_RS27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator RepA"
                     /protein_id="WP_032495102.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSRF
                     DFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECGLA
                     TESDAGVLSITRATRALTSLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDVSEV
                     AVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARA
                     RRDADRERQDIITLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRNYSRLA
                     TASP"
                     /name="repA"
     gene            1559..1699
                     /locus_tag="PBU98_RS27405"
     CDS             1559..1699
                     /locus_tag="PBU98_RS27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001333237.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLPC
                     R"
                     /name="hypothetical protein"
     gene            1797..2450
                     /locus_tag="PBU98_RS27410"
     CDS             1797..2450
                     /locus_tag="PBU98_RS27410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000616807.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLFF
                     SHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALALPA
                     EPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITS
                     LLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
                     /name="CPBP family intramembrane glutamic endopeptidase"
     gene            2543..2800
                     /gene="pemI"
                     /locus_tag="PBU98_RS27415"
     CDS             2543..2800
                     /gene="pemI"
                     /locus_tag="PBU98_RS27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="WP_000557619.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIVE
                     PYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
                     /name="pemI"
     gene            2733..3134
                     /gene="pemK"
                     /locus_tag="PBU98_RS27420"
     CDS             2733..3134
                     /gene="pemK"
                     /locus_tag="PBU98_RS27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_001398199.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQGT
                     RPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTID
                     MKARGGKRLERVPETIMNEVLGRLSTILT"
                     /name="pemK"
     gene            3383..>3798
                     /locus_tag="PBU98_RS27425"
                     /pseudo=""
     CDS             3383..>3798
                     /locus_tag="PBU98_RS27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<3867..>4379)
                     /locus_tag="PBU98_RS27430"
                     /pseudo=""
     CDS             complement(<3867..>4379)
                     /locus_tag="PBU98_RS27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            complement(4445..5149)
                     /locus_tag="PBU98_RS27435"
     CDS             complement(4445..5149)
                     /locus_tag="PBU98_RS27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <5207..5305
                     /locus_tag="PBU98_RS27440"
                     /pseudo=""
     CDS             <5207..5305
                     /locus_tag="PBU98_RS27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /name="conjugal transfer protein TrbG"
     gene            5302..5817
                     /gene="traV"
                     /locus_tag="PBU98_RS27445"
     CDS             5302..5817
                     /gene="traV"
                     /locus_tag="PBU98_RS27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="WP_000809902.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANEK
                     AKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPRPL
                     FTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVIKPS
                     AWGKPRIN"
                     /name="traV"
     gene            5952..6173
                     /gene="traR"
                     /locus_tag="PBU98_RS27450"
     CDS             5952..6173
                     /gene="traR"
                     /locus_tag="PBU98_RS27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="WP_001278692.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPVP
                     EARRKIFPGVTLCVECQAYQERQRKHYA"
                     /name="traR"
     gene            6333..8960
                     /gene="traC"
                     /locus_tag="PBU98_RS27455"
     CDS             6333..8960
                     /gene="traC"
                     /locus_tag="PBU98_RS27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="WP_015059012.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLP
                     YRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLMSSQ
                     LVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRVFFS
                     YCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNPDSLYP
                     KRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPEIAFLW
                     NMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPS
                     VEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELI
                     SPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLA
                     PTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGGFAVVFDMGD
                     GYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNGNLDEVHEGLL
                     LQAVRASWLAKKKQARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIVLLDQYTANGTY
                     GRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRNLKKLNV
                     IDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNS
                     SYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRL
                     FVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGPEMASLEAWLEEHEKY
                     RSVA"
                     /name="traC"
     gene            8957..9343
                     /gene="trbI"
                     /locus_tag="PBU98_RS27460"
     CDS             8957..9343
                     /gene="trbI"
                     /locus_tag="PBU98_RS27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="WP_015059013.1"
                     /translation="MTTTQKTTDVTARRCSHWWWTVPGCLAMVLLNAAVSYGIVRLNAP
                     VTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVSPA
                     VVQGAPDITREIQQDIARRMRAEP"
                     /name="trbI"
     gene            9340..9972
                     /gene="traW"
                     /locus_tag="PBU98_RS27465"
     CDS             9340..9972
                     /gene="traW"
                     /locus_tag="PBU98_RS27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="WP_001203735.1"
                     /translation="MRCRGLIALLIWGQSVTAADLGTWGDLWPVKEPDMLTVIMQRLTA
                     LEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEGRV
                     FARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEMQKS
                     LDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
                     /name="traW"
     gene            9969..10961
                     /gene="traU"
                     /locus_tag="PBU98_RS27470"
     CDS             9969..10961
                     /gene="traU"
                     /locus_tag="PBU98_RS27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="WP_000830838.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFPL
                     SLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCMVN
                     LGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLDIAY
                     LSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGSQGSM
                     YPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILPKERWR
                     YQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
                     /name="traU"
     gene            10988..11296
                     /locus_tag="PBU98_RS27475"
     CDS             10988..11296
                     /locus_tag="PBU98_RS27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000412447.1"
                     /translation="MEKKLSSKYIHMYMSISRVFIRLTGNVLKLFPFAMVICPVVWFYL
                     CPEHIISDIVNDWQDADTLEKILLVKLYLKTVLILTVVSVVVLYLSSDKFMPVKRRK"
                     /name="hypothetical protein"
     gene            11359..11880
                     /locus_tag="PBU98_RS27480"
     CDS             11359..11880
                     /locus_tag="PBU98_RS27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059014.1"
                     /translation="MGFFSFLRKISNKEKGVVIYEKQWFSLLEKVSAFDEVRQWAESNG
                     GAYYPLLECRRLTDSENVRKSEDILNRLEFIDRRFREFISIMSEHINNDKTKSMWEREL
                     SEYPPEKIYQVRREGEHLSLKNYGDDITLNRDTAVATVQITVSRKCITQEEMDELYALS
                     ERLKEKCAGN"
                     /name="hypothetical protein"
     gene            11907..12545
                     /gene="trbC"
                     /locus_tag="PBU98_RS27485"
     CDS             11907..12545
                     /gene="trbC"
                     /locus_tag="PBU98_RS27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TrbC"
                     /protein_id="WP_000087532.1"
                     /translation="MSRKIFELFALTAGLFISAHASENVNTPENRQFLKQQETLSQQLR
                     EKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPEEG
                     LKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGIRSV
                     PALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKSQ"
                     /name="trbC"
     gene            12542..12913
                     /locus_tag="PBU98_RS27490"
     CDS             12542..12913
                     /locus_tag="PBU98_RS27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000056336.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPPY
                     IAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTFNSDFQLCFRLQPEGPHHWYHR
                     YCRRSLRADFRTLVRLVNP"
                     /name="hypothetical protein"
     gene            12939..13361
                     /locus_tag="PBU98_RS27495"
     CDS             12939..13361
                     /locus_tag="PBU98_RS27495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="WP_001229309.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSEQ
                     CMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVERG
                     RRSSVNFRTAERFGLIQVVNKPVVFWFEQYQRESEV"
                     /name="HNH endonuclease signature motif containing protein"
     gene            13358..15208
                     /gene="traN"
                     /locus_tag="PBU98_RS27500"
     CDS             13358..15208
                     /gene="traN"
                     /locus_tag="PBU98_RS27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="WP_015059015.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGSDFARQIQGQGTGSIQGF
                     KPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKDIL
                     SPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEFCTRTARI
                     TGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFLNSRY
                     TFMNSTFNVGLVNGQSGTFPLSGATGLQVTQGQVLTGSGCTANGNCLSGGNGDRQVYEK
                     LASGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKTGVMEGK
                     PWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLHEYATYSC
                     ESKTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVAALNGVDVR
                     AFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLTVSVGEFCSK
                     KVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGITVDELQKIQFD
                     RLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
                     /name="traN"
     gene            15235..15492
                     /locus_tag="PBU98_RS27505"
     CDS             15235..15492
                     /locus_tag="PBU98_RS27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="WP_053913405.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTANGMLA
                     AAFVILYSGVLLFILYFCFSALTDLQKPDERKNDERNEDE"
                     /name="conjugal transfer protein TrbE"
     gene            15485..16228
                     /gene="traF"
                     /locus_tag="PBU98_RS27510"
     CDS             15485..16228
                     /gene="traF"
                     /locus_tag="PBU98_RS27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     protein TraF"
                     /protein_id="WP_015059017.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAAP
                     RQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAML
                     AHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIARLAEHYGIMFFYRGQEPIDGQLAQV
                     INGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPL
                     SYGFISQDDLAKQFLNVSEDFKPNF"
                     /name="traF"
     gene            16389..16625
                     /gene="traQ"
                     /locus_tag="PBU98_RS27515"
     CDS             16389..16625
                     /gene="traQ"
                     /locus_tag="PBU98_RS27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="WP_000270010.1"
                     /translation="MWVFSLGVWFHIVARLVYSKPWMAFFLAELIAAILVLFGAYQVLD
                     AWIARVSREEREALEARQQAMMAEQQEGGHVSH"
                     /name="traQ"
     gene            16612..17157
                     /gene="trbB"
                     /locus_tag="PBU98_RS27520"
     CDS             16612..17157
                     /gene="trbB"
                     /locus_tag="PBU98_RS27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin assembly
                     thiol-disulfide isomerase TrbB"
                     /protein_id="WP_000059817.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAAQPAQPAAD
                     TSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSVFP
                     YTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDAAGF
                     MARMDTVLQMYGGKKGAK"
                     /name="trbB"
     gene            17147..17461
                     /gene="trbJ"
                     /locus_tag="PBU98_RS27525"
     CDS             17147..17461
                     /gene="trbJ"
                     /locus_tag="PBU98_RS27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="WP_001384255.1"
                     /translation="MRNKQVVLLIAGISGIATGIIVSLNIPFIRQGLFYPASPVEIVVS
                     LCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSVISEETYNDSKQKESGIQNQKN
                     "
                     /name="trbJ"
     gene            17415..17807
                     /gene="trbF"
                     /locus_tag="PBU98_RS27530"
     CDS             17415..17807
                     /gene="trbF"
                     /locus_tag="PBU98_RS27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="WP_000126338.1"
                     /translation="MTANKKNQEFKIRKIRRNIEYSFRDSDRYFDLFIVFLVAGIVLWA
                     VMHVIFDVCIDSWMADPKLLNFQYMWNVLMKVIPFTLWALAAGFLVTFFLSPMCELIFG
                     NIMIFLLKRRMRRENTLREGSNNASH"
                     /name="trbF"
     gene            17794..19167
                     /gene="traH"
                     /locus_tag="PBU98_RS27535"
     CDS             17794..19167
                     /gene="traH"
                     /locus_tag="PBU98_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="WP_000944325.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQS
                     GVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQLQR
                     FVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGGLFP
                     RTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNINIMW
                     NALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGGTAKIY
                     HCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGFISSTTI
                     PVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYDEAVIEHI
                     NDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGGSTL"
                     /name="traH"
     gene            19164..21980
                     /gene="traG"
                     /locus_tag="PBU98_RS27540"
     CDS             19164..21980
                     /gene="traG"
                     /locus_tag="PBU98_RS27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="WP_323740314.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAVA
                     VMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPLSL
                     TTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDYVQN
                     CVLGDIYLNHKYTLEDLMASGDPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLKDRLN
                     LDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNVTMNAL
                     KEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVGITIGIF
                     PLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPVVLSELSQ
                     IQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTASAAGSVVDG
                     NYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDASGAMSRLPV
                     GINATRQIAAAQQEMSREASNRAESALHGFSSSIASAWNTLSQFGSNRGSSDSVTGGAD
                     STMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLYGDAYAKGHLGI
                     SVLGNGGGVGLQAGAKASIVGSDLDSHEASSGSRASHDARHDIDARATQDFKEASDYFT
                     SRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQ
                     MSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNAWRE
                     SRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRNDVKHQVDNMVTEYKGN
                     IGDTQNSIRGEENIVRGQYSELQNHHKTEALTQNNKYNEEKLAQERIPGADSPKELLEK
                     AKSYQHKE"
                     /name="traG"
     gene            22013..22534
                     /gene="traS"
                     /locus_tag="PBU98_RS27545"
     CDS             22013..22534
                     /gene="traS"
                     /locus_tag="PBU98_RS27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632670.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="WP_000632668.1"
                     /translation="MITQQIISSELEVLKKHIDSGDIRIPSLWQGLKPGFIIMGWMIFC
                     PLLMSFLITQKTSETLTAVLAGGWLGLIILFIVARIRMLYFSLPEEFLKTSSVMRIISS
                     KLKVYFIVYMGIIFLWSFLGGGIIYGFGAILVTVIMAFLIQLDIGRYQFVGVIDAINSY
                     VKNKKLSRVK"
                     /name="traS"
     gene            22559..23290
                     /gene="traT"
                     /locus_tag="PBU98_RS27550"
     CDS             22559..23290
                     /gene="traT"
                     /locus_tag="PBU98_RS27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="WP_021559024.1"
                     /translation="MKTKKLMMVALVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIWL
                     EPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKM
                     DLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVEDVN
                     YTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNL
                     KFEEAKPVLEDQLAKSIANIL"
                     /name="traT"
     gene            23493..24230
                     /locus_tag="PBU98_RS27555"
     CDS             23493..24230
                     /locus_tag="PBU98_RS27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059020.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQNNTVTTKDYLYSNNID
                     TDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNSLKPEAVSCNL
                     LSLCVLAPVFFTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQSPAD
                     KYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFVQIQFPHGSDWSEVLRLTDIVTEKV
                     SSGRAAGGYHHRHTGYTWSVSVN"
                     /name="hypothetical protein"
     gene            24281..26506
                     /gene="traD"
                     /locus_tag="PBU98_RS27560"
     CDS             24281..26506
                     /gene="traD"
                     /locus_tag="PBU98_RS27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="WP_000009363.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVL
                     WVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWC
                     GEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARML
                     KKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRS
                     GEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGS
                     GRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISI
                     RAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVIS
                     MWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYA
                     QLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQYSYGADPVR
                     DGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARPKVAPEFIPRD
                     INPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQ
                     QPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
                     /name="traD"
     gene            26506..31776
                     /gene="traI"
                     /locus_tag="PBU98_RS27565"
     CDS             26506..31776
                     /gene="traI"
                     /locus_tag="PBU98_RS27565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="WP_061093447.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQG
                     SVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLID
                     AHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVA
                     NVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHG
                     MWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTL
                     KETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVL
                     ARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDI
                     MKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMARE
                     QGREVQIIAADRRSQMNLKQDERLSGELITGRRQLPEGMAFTPGSTVIVDQGEKLSLKE
                     TLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNIYRWQGGEQRPATIISEPDR
                     NVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALS
                     PVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDVQGETQVVR
                     ISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDVMTVVVPGRA
                     EPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMDKATLNGLARSGRDV
                     RLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQKSALNTPAQQAIHLA
                     LPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDAQIKRGDLLHVDVAKGYGTDL
                     LVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETPDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLH
                     DTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQ
                     EGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEARE
                     QTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWETHR
                     DALALVDNVYHRIAGISRDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGD
                     RMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPAQEQAEQHIDLAYAITA
                     HGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAV
                     QKGTAHDVLEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGR
                     KYPQPYVALPAFDRNGKSAGIWLNPLTSDDGNGLRGFSGEGRVKGSGDAQFVALQGSRN
                     GESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAG
                     NGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQVRE
                     RAAITERETALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD
                     "
                     /name="traI"
     gene            31796..32542
                     /gene="traX"
                     /locus_tag="PBU98_RS27570"
     CDS             31796..32542
                     /gene="traX"
                     /locus_tag="PBU98_RS27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="WP_000205770.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLMV
                     LDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAY
                     YLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLM
                     LAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKS
                     LPRFWPGDFFPVFYACHLAVLGVLAL"
                     /name="traX"
     gene            32597..33157
                     /locus_tag="PBU98_RS27575"
     CDS             32597..33157
                     /locus_tag="PBU98_RS27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="WP_000139328.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLAE
                     KAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRDVL
                     LEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEAYAA
                     ARLDKIRRQNRIKAELQAVLDEK"
                     /name="fertility inhibition protein FinO"
     gene            33289..33489
                     /locus_tag="PBU98_RS27580"
     CDS             33289..33489
                     /locus_tag="PBU98_RS27580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015059022.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVSLEVRGFRKEAAEAWLLAARV
                     APRTDWQQFARQRAERCQRRR"
                     /name="hypothetical protein"
     gene            33875..34474
                     /locus_tag="PBU98_RS27585"
     CDS             33875..34474
                     /locus_tag="PBU98_RS27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="WP_032083981.1"
                     /translation="MATGKSEKQQVIAFIDYENCSNLTEISLNIYTELIIFAGAKQNNV
                     ALPVNAFPEAVKITLRHVSEVSRNNVDFHLVLELGQCVCHYGPEKEYHIVSADKGYDGI
                     VRTLQARGIRCRRVSPDKASSVKMAAPDMDIVIRWANKIQQAAREVRNMPVTEKTFNNY
                     LKSQMRGEWRDVLTRGIRDELVRRGVMKIQGNKIIW"
                     /name="PIN domain-containing protein"
     gene            34536..34868
                     /locus_tag="PBU98_RS27590"
     CDS             34536..34868
                     /locus_tag="PBU98_RS27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152916585.1"
                     /translation="MAFISMTRQKAPYRYASKTRLSIRRTDSGSAGCYVTVAVRGKQEF
                     IIAELDSEIRKIRLKLTDSYEEGVRLSSGTFTLPARFCREILPDDVRSITIILEKSDDE
                     WWYGSY"
                     /name="hypothetical protein"
     gene            35173..35322
                     /locus_tag="PBU98_RS27595"
     CDS             35173..35322
                     /locus_tag="PBU98_RS27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="WP_001312851.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAY
                     EARK"
                     /name="Hok/Gef family protein"
     gene            35606..35854
                     /locus_tag="PBU98_RS27600"
     CDS             35606..35854
                     /locus_tag="PBU98_RS27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="WP_000083837.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKELR
                     VFIPAALKAQLQEMCDAEGVTQAEMIAELIKQKSAFS"
                     /name="replication regulatory protein RepA"
     Typing          104..858
                     /name="IncFIA"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(35910..36165)
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.611"
                     /coverage="99.0"
                     /evalue="4.89e-130"
     Typing          26507..31773
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.437"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5303..5814
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="97.076"
                     /coverage="100.0"
                     /evalue="3.89e-98"
     Typing          6334..8954
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="90.503"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          9341..9969
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.048"
                     /coverage="97.0"
                     /evalue="2.88e-135"
     Typing          9970..10925
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.746"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11908..12542
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="92.925"
                     /coverage="100.0"
                     /evalue="5.33e-130"
     Typing          13359..15205
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="96.753"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          19009..21959
                     /name="MPF_F"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="90.264"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          24282..26503
                     /name="MPF_Unknown"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.46"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgactgatc ttcaacaaac ttattaccgc caggtaaaga acccgaatcc ggtgtttaca
       61 ccccgtgaag gtgccggaac gctgaagttt tgcgaaaaac tgatggaaaa ggcggtaggc
      121 ttcacctccc gttttgattt cgccattcat gtggcacatg cccgttcgcg tggtctgcgt
      181 cggcgcatgc caccggtact gcgtcgtcgg gctattgatg cgctgctgca ggggctctgt
      241 tttcactatg atccgctggc caaccgtgtc cagtgctcca tcaccacgct ggccattgag
      301 tgtggactgg cgacggagtc tgatgccgga gtactctcca tcacccgtgc cacccgtgcc
      361 ctgacgtccc tggctgagct gggtctgatt acctaccaga cggaatatga cccgcttatt
      421 ggttgcaaca ttccgaccga tatcacgttc acaccggcgc tgtttgccgc tctcgatgtg
      481 tctgaggtgg cggtggcttc cgcacgccgc agccgtgtgg aatgggaaaa cagacaacgc
      541 aaaaagcagg ggctggatac cctgggcatg gatgaactga tagcgaaagc ctggcgtttt
      601 gttcgtgagc gtttccgcag ttaccagaca gagcttaagt cccggggaat aaagcgtgcc
      661 cgtgcgcgtc gtgatgcgga cagggaacgt caggatatta tcaccctggt gaaacggcag
      721 ctgacgcgcg aaatcgcgga agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa
      781 gtggagcgtc gtgtgaagga gcgcatgatt ctgtcacgta accgcaatta cagccggctg
      841 gccacagctt ccccctgaaa gtgacctctt ctgaataatc cggcccgaac cggaggcatt
      901 cgcacgcctg aagcctgcca gcgaacaaaa aatcagcacc acatacaaaa aacaacctca
      961 ctatccacct tctggagcat ccggttcccc ctgtttttaa tacaaaatgc gcctcagcga
     1021 cggggaattt tgcttatcca catttaactg caagggactt ccccataagg ttacaaccgt
     1081 tcatgtcata aagcgccagc cgccagtctt acagggtgca atgtatcttt taaacacctg
     1141 tttatatctc ctttaaacta cttaattaca ttcatttaaa aagaaaacct attcactgcc
     1201 tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc gaccggagcc
     1261 actttagtta caacacacaa aaacaacctc cagaaaaacc ccggtccagc gcagaaccga
     1321 aaccacaaag cccctccctc ataactgaaa agcggccccg ccccggccca aagggccgga
     1381 acagagtcgc ttttaattat gaatgttgta actacatctt catcgctgtc agtcttctcg
     1441 ctggaagttc tcagtacacg ctcgtaagcg gccctcacgg cccgctaacg cggagatacg
     1501 ccccgacttc gggtaaaccc tcgtcgggac cactccgacc gcgcacagaa gctctctcat
     1561 ggctgaaagc gggtatggtc tggcagggct ggggatgggt aaggtgaaat ctatcaatca
     1621 gtaccggctt acgccgggct tcggcggttt tactcctgta tcatatgaaa caacagagtg
     1681 ccgccttcca tgccgctgat gcggcatatc ctggtaacga tatctgaatt gttatacatg
     1741 tgtatatacg tggtaatgac aaaaatagga caagttaaaa atttacaggc gatgcaatga
     1801 ttcaaacacg taatcaatat ctgcagttta tgctggttat gctggctgca tggggcatta
     1861 gttggggagc cagatttgtc atggagcagg ccgttctgct ttatggatca ggaaaaaact
     1921 atttgttctt cagtcatggt actgttctga tgtacctgct gtgtgttttc ctggtatacc
     1981 gccgttggat agctccgcta ccggtcgttg gtcagctgcg caacgttggc gtaccgtggc
     2041 tggtcggtgc gatggccgtg gtgtatgtcg gtgtatttct gctcggtaag gcgctggctc
     2101 tgcctgctga gccatttatg acgaaacttt ttgccgataa gtccatacct gacgtgatcc
     2161 tgacgttgct gaccatcttt atccttgccc cgttgaatga ggaaacgctg ttccggggga
     2221 ttatgctgaa cgtcttccgt tcacggtact gctggacgat gtggctgggg gcgctgataa
     2281 cgtcgttgtt gttcgtcgcc gcgcacagcc agtatcagaa cctgctgaca ctggcagaac
     2341 tgttcctggt ggggttgatt acatcagtgg ccaggatcag aagtggtggc ctgctgctgc
     2401 cggtattgct gcatatggaa gcaaccacgc tgggtttact gtttggttga aagttatatt
     2461 tttattaaac attgtgcgtt aaagcctggt gtgttttttt agtggatgtt atatttaaat
     2521 ataactttta tggaggtgaa gaatgcatac cacccgactg aagagggttg gcggctcagt
     2581 tatgctgacc gtcccaccgg cactgctgaa tgcgctgtct ctgggcacag ataatgaagt
     2641 tggcatggtc attgataatg gccggctgat tgttgagccg tacagacgcc cgcaatattc
     2701 actggctgag ctactggcac agtgtgatcc gaatgctgaa atatcagctg aagaacgaga
     2761 atggctggat gcaccggcga ctggtcagga ggaaatctga catggaaaga ggggaaatct
     2821 ggcttgtctc gcttgatcct accgcaggtc atgagcagca gggaacgcgg ccggtgctga
     2881 ttgtcacacc ggcggccttt aatcgcgtga cccgcctgcc tgttgttgtg cccgtaacca
     2941 gcggaggcaa ttttgcccgc actgccggct ttgcggtgtc gttggatggt gttggcatac
     3001 gtaccacagg tgttgtacgt tgcgatcaac cccggacaat tgatatgaaa gcacggggcg
     3061 gaaaacgact cgaacgggtt ccggagacta tcatgaacga agttcttggc cgcctgtcca
     3121 ctattctgac ttgaacatgg ggtttgaggg gcaactggat gaaaacgtac gatttagggc
     3181 actaaaaccg ctgttgtccc accattctgg tgattcccaa acgttatttg gctaaaaagt
     3241 agttttgatg tggtttattt tccgaattcc aagcgcagcc ctactttctt gtgcgtattt
     3301 gcgtgttttg cgcagttttg aagttccggt gatgctgcca acttactgat ttagtgtatg
     3361 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     3421 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
     3481 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
     3541 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaaccg cccgcattat
     3601 gggcgttggc ctcaacacga ttttccgcca tttaaaaaac tcaggccgca gtcggtaacc
     3661 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtgggga
     3721 tacgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag gctccggaag
     3781 acggttgttg cgcacgtatt ggggtctgac gctcagtgga acgaaaactc acgttaagca
     3841 acgttttctg cctctgacgc ctcttttaat ggtctcagat gtcctttggt caccagttct
     3901 gccagcgtga aggaataatg gccgagcata ttgatatgtc cgtggcaaag cggggagagg
     3961 cgtgcgatat cttcatcatt cagtgtttca ccctgcgccc ggagatgatc cagggctgcc
     4021 tgcatataaa tagtgttcca taacacgacg gcgttagtga ccagccccag tgcgcccagt
     4081 tgatcttcct gaccgtcggt atatcgtttt cttatctcac ctttttgacc gtgacagatg
     4141 gctctggcaa cggcatggcg actttctccc cgattaagct gggtcagaat gcgccggcgg
     4201 taatcttcat catcaatata attaagcaga tacagcgttt tgttgatgcg ccccacttca
     4261 atgattgcct gagtcagtcc ggaaggacgt tcacttttca gcaatgaacg gaccagcact
     4321 gaagcctgta ctttgcccag cttcagggag ccagcggtcc ggatcatttc gtcccactga
     4381 aggactattt ttggcactgt tgcaaagtta gcgatgaggc agccttttgt cttattcaaa
     4441 ggccttacat ttcaaaaact ctgcttacca ggcgcatttc gcccagggga tcaccataat
     4501 aaaatgctga ggcctggcct ttgcgtagtg cacgcatcac ctcaatacct ttgatggtgg
     4561 cgtaagccgt cttcatggat ttaaatccca gcgtggcgcc gattatccgt ttcagtttgc
     4621 catgatcgca ttcaatcacg ttgttccggt acttaatctg tcggtgttca acgtcagacg
     4681 ggcaccggcc ttcgcgtttg agcagagcaa gcgcgcgacc ataggcgggc gctttatccg
     4741 tgttgatgaa tcgcgggatc tgccacttct tcacgttgtt gaggatttta cccagaaacc
     4801 ggtatgcagc tttgctgtta cgacgggagg agagataaaa atcgacagtg cggccccggc
     4861 tgtcgacggc ccggtacaga tacgcccagc ggccattgac cttcacgtag gtttcatcca
     4921 tgtgccacgg gcaaagatcg gaagggttac gccagtacca gcgcagccgt ttttccattt
     4981 caggcgcata acgctgaacc cagcggtaaa tcgtggagtg atcgacattc actccgcgtt
     5041 cagccagcat ctcctgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg
     5101 cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca
     5161 tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg ccgtttttga
     5221 aaatcctgac attgtaaaaa cctgtacggt cagggatttg aagaatgatt ttgtgaactg
     5281 cgatgagaaa ggtgagggac agtgaaacag atttctttat ttattcctct gctggggacc
     5341 ttattacttt ccggctgtgc cggaaccagt acggaatttg agtgtaacgc caccacatcc
     5401 gatacctgta tgacgatgga gcaggccaat gagaaggcca aaaaactgga gcagtcctca
     5461 gaagcaaagc cggttgcggc atcactgccg cgcctggctg aagggaactt ccggacaatg
     5521 ccggtgcaga ccgtcaccgc gaccactccg tccggcagca gacctgcggt gacagcacat
     5581 ccggagcaga aactgctggc accacgcccg ctgtttaccg ctgcccggga agtgaaaacg
     5641 attgttccgg tcagttcagt tgcgccggta acacctcccc gtccgctaag aacgggtgag
     5701 cagacggctg cattatggat agcgccttat attgataatc aggatgttta tcatcagcca
     5761 tccagcgtgt tttttgttat taaaccgtct gcgtggggaa aaccacgtat taattaactg
     5821 gccctgaagt tgtatatcag ccggcaatgc cggcgggctt tctgtattca aaatctgcct
     5881 gtaaaacgag tgccctgtgc gtgaaaaggg atgggcggta gcgtgtcaga acatgtcggg
     5941 aggaatattc cgtgagtgat gaagccgatg aagcatattc agtgacagaa caactgacca
     6001 tgacaggaat aaaccggata cgccagaaaa taaatgctca tggtattcct gtttatctct
     6061 gtgaagcatg cggaaatcct gttccggaag cccggcggaa aatatttccc ggtgtgacgt
     6121 tgtgcgttga atgtcaggcg tatcaggaaa gacagagaaa acattatgca taagtcagtc
     6181 gcagaacata gtgatttaac tccggatgaa catgagtgga tattcagaaa acagaaatct
     6241 ttgtatatgc gccgggaaat ggcgcgttaa ttacaggtat tcccttcatg gctaccgcat
     6301 ttctcgcttt atttttcaac taaggaattc atgtgaataa cccacttgag gccgtcactc
     6361 aggcggttaa ctccctcgtc acagcactga aactgcctga cgaatccgca aaggccaatg
     6421 aagttctggg cgaaatgagc ttcccgcagt tcagccgtct gctgccgtac cgtgattaca
     6481 accaggaatc cggtctgttc atgaatgaca ccacgatggg ctttatgctg gaagccattc
     6541 ccatcaatgg ggcgaatgag tccattgtgg aggcgctcga ccacatgctg cgcaccaaac
     6601 tgccgcgcgg agtgccgttc tgcatccatc tgatgtccag tcagcttgtt ggtgacagga
     6661 ttgaatacgg gctgcgtgag ttctcctggt caggtgaaca ggctgaacgg tttaacgcca
     6721 ttacccgtgc ctattatatg aacgcggcag cgacacagtt tccgctgccg gaggggatga
     6781 atctgcccct gaccctgcgc cattaccggg tattcttctc gtactgttct ccctcgaaga
     6841 aaaaaagccg ggctgacatt ctggaaatgg aaaacctggt gaaaatcatc cgggcgtcgt
     6901 tacagggggc cagtatcacc acacaggcgg tggatgcaca ggcctttatc gatattgtcg
     6961 gggagatgat taaccataac ccggactccc tgtacccgaa aagacgtcag ctggacccgt
     7021 attctgatct gaattatcag tgtgtggagg acagttttga tctgaaggtc cgggcggatt
     7081 acctgacgct ggggctgcgt gagaacggca ggaacagcac agcccgtatc ctgaatttcc
     7141 atctggcccg taacccggaa atcgccttcc tgtggaacat ggccgataac tacagcaatc
     7201 tgctgaaccc ggaaatgtcc atttcctgcc cgttcatcct gacgctgaca ctggtggtgg
     7261 aagatcaggt gaaaacccac agtgaagcca acctgaagta catggacctg gagaaaaagt
     7321 cgaagacctc ctatgccaaa tggtttccgt ccgtggagaa agaagctaag gagtgggggg
     7381 aactgcgcca gcggctgggc tccggtcagt cttctgtcgt gtcctacttc cttaacatca
     7441 cagcattctg caaggataac aatgaaacgg ctctggaagt ggagcaggac attctgaaca
     7501 gctttcgtaa aaacggtttt gagctgattt caccgcgctt taaccacatg cgcaatttcc
     7561 tgacctgcct gccctttatg gccgggaaag ggctgtttaa acagctgaaa gaggccggtg
     7621 tggttcagcg cgcagagagc tttaatgtgg ccaacctgat gccgttagtg gcggataacc
     7681 ccctgacacc ggcaggcctg ctggcaccca cctaccgtaa ccagctggcg tttatcgata
     7741 tcttctttaa ggggatgaat aacaccaact acaacatggc ggtctgtgga acatccgggg
     7801 ccggtaaaac cgggttgata cagccactta tccgcagcgt gctggactcc gggggctttg
     7861 ccgtggtgtt cgacatgggg gatggctaca agtctttgtg tgagaacatg gggggcgtat
     7921 atctggacgg tgaaaccctg cgctttaacc cgtttgcgaa catcactgat attgaccagt
     7981 cagcggagcg tgtccgtgac cagttgtcgg tgatggccag ccccaacggt aacctggatg
     8041 aagtgcatga aggtctgttg ctgcaggcgg tcagagcctc ctggctggct aaaaagaagc
     8101 aggcgcgcat tgatgatgtg gtggatttcc tgaaaaacgc ccgtgataac gaccagtatg
     8161 tcgagtcacc gaccatccgc agccgtctgg acgaaatgat tgtgctgctt gaccagtaca
     8221 ctgccaacgg cacttacggc cggtatttta actctgatga accgtccctg cgggatgatg
     8281 ccagaatggt ggtgctggag ctgggtgggc tggaagaccg tccgtcactg ctggttgcgg
     8341 tgatgttcag ccttatcatt tacatcgaga accggatgta ccgcacgccg cgtaacctca
     8401 agaaactgaa cgtcattgat gaaggctggc gtctgctgga cttcaaaaat cgtaaagttg
     8461 gtgagtttat tcaaaaaggt taccgcacct gtcgccgtca taccggtgcc tatatcacca
     8521 tcacacagaa catcgtcgac tttgactctg acaaggcatc cagcgcagcc cgcgcggcat
     8581 ggggtaactc ctcctacaaa attatcctca aacagagtgc gaaggagttc gcgaaataca
     8641 accagctgta tccggaccag ttcctgccac tgcagcgcga catgattggt aagtttggtg
     8701 cggccaaaga ccagtggttc agttccttcc tgctgcaggt ggagaaccac tcctcctggc
     8761 accgtctgtt tgtggacccg ttaagccgcg ccatgtacag ctctgacggc ccggattttg
     8821 agtttgtgca gcagaagcgt aaagaggggc tgagtattca tgaggcggtg tggcagctgg
     8881 cgtggaagaa gtcagggccg gagatggctt cgctggaagc ctggctggaa gaacatgaga
     8941 aatacaggag tgttgcatga ccacaacgca aaaaacgact gatgttacag cccgtcgatg
     9001 cagccactgg tggtggacgg tgccgggatg cctggcaatg gtgttactga acgcagccgt
     9061 cagttacggc attgtcagac tgaacgcacc ggtgactgtc gccttcaaca tgaagcagac
     9121 cgtggatgcg ttttttgaca gcgccagtca gaaacaactg tcagaagcac agtcaaaagc
     9181 cctttcagcg cgttttaata cggcactgga agccagcctg caggcgtggc agcagaaaca
     9241 ccacgcggtc attctggtgt cgcctgccgt ggtacagggg gcaccggata tcacccggga
     9301 aatccagcag gatatcgccc ggcgaatgag ggcggaacca tgagatgccg ggggctgatt
     9361 gccctgctga tatgggggca gagtgtgacc gccgccgatc ttggtacctg gggcgatctg
     9421 tggccggtaa aggagccgga catgctgacg gtgatcatgc agcgcctgac ggcactggag
     9481 cagtccggtg agatgggccg gaaaatggat gcctttaagg agcgggtgat ccgcaacagt
     9541 ctgcggcccc ctgccgtgcc cggtatcgga cgcacggaga aatacggcag ccggttgttt
     9601 gacccgtccg tcagactggc tgcggatatc cgggataacg aagggcgggt gtttgcccgt
     9661 cagggtgaag tgatgaatcc gctgcagtat gttcctttta accagacgct gtatttcatc
     9721 aatggcgatg atccggcgca ggtggcctgg atgaaacgcc agacgccgcc cacactggag
     9781 agcaaaatta tcctcgtgca gggcagtatc ccggagatgc agaagtctct ggacagccgt
     9841 gtttactttg accagaacgg tgtgctctgc cagcgcctcg gcattgatca ggtcccggca
     9901 cgggtgagcg ccgttcccgg cgatcgcttc ctgaaggtgg aatttattcc ggcagaggag
     9961 ggcagaaaat gaagcgaagc ctgtggctgc tgatgttatt ccttctcgcc ggtcatgtcc
    10021 ctgcagcgtc tgcggattct gcctgtgaag ggcgttttgt aaacccgatc acggatatct
    10081 gctggagctg tattttcccg ctctcgctgg gcagtatcaa agtcagtcag ggtaaggtcc
    10141 ccgacacggc gaacccgtcg atgcccattc agatttgtcc ggcaccgccg ccgctgttca
    10201 ggcgtatcgg gctggccatt ggttactggg agccgatggc gctgacggat gtcacccggt
    10261 caccgggatg catggtgaac ctgggcttca gcctgccagc ttttggtaaa acggcacagg
    10321 gaacggcgaa aaaggatgag aagcaggtga acggggcgtt ctatcacgtt cactggtaca
    10381 aatacccgct gacgtactgg ctgaacatca tcacatcgct gggctgtctg gaaggtggtg
    10441 acctggatat cgcttatctt tctgaaattg accccacctg gacggacagc agcctgacca
    10501 ccattctcaa tccggaagct gtcatctttg ccaatccgat agcacagggg gcctgtgcag
    10561 cagatgccat tgccagcgcc tttaatatgc ctctcgatgt tctgttctgg tgtgccggtt
    10621 cgcagggcag catgtacccg ttcaatggct gggtgagtaa tgagtccagt ccgttgcagt
    10681 cctccctgct ggtcagtgaa cgcatggcgt tcaagctgca ccgtcagggc atgattatgg
    10741 aaaccatcgg gaaaaataac gccgtctgta atgaatatcc gtccccaatc ctgcccaaag
    10801 aacgctggcg ttatcagatg gtgaatatgt atccggacag cggacagtgc cacccgttcg
    10861 ggcgcagtgt gatgcgctgg gagacgggaa aaaatccgcc caacacaaag aaaaacttcg
    10921 gctacctgat gtggcgtaaa cgtaactgtg tatttctgta acttaaacaa aaagaggaat
    10981 attttaaatg gaaaagaaat taagcagtaa atacattcat atgtatatga gtatatcccg
    11041 agtgttcatt cgtttgacag gaaatgtatt aaaactgttt ccttttgcta tggtgatatg
    11101 ccctgtggtg tggttttatc tttgccctga acatattatc tcagacattg ttaacgactg
    11161 gcaggatgca gatacactgg aaaaaatact gcttgtcaaa ctgtatctga agacggttct
    11221 tattctgaca gtggtaagcg ttgttgtctt gtatctttcg tcagataagt ttatgcctgt
    11281 taaacgcagg aaatgagtct ggtggttctc ataaatataa ctgtgtgtgc atgtgtacaa
    11341 tataagtaaa ggtgcgttat gggatttttc tcgtttttac gaaagataag taataaagaa
    11401 aaaggggtgg ttatctatga gaagcagtgg ttctcactgc tggaaaaggt gagtgctttt
    11461 gatgaggtca gacaatgggc tgaaagtaat ggtggagcat attatcctct gctggagtgt
    11521 cgtcggctta cagacagtga aaatgtcagg aaatcagagg atatactgaa ccgactggag
    11581 tttattgaca gaagatttcg ggaattcatc agcattatgt ctgaacatat taataatgat
    11641 aaaactaaat ccatgtggga gagggaattg tctgaatatc caccggaaaa aatataccag
    11701 gtccggagag agggagaaca tctgtcatta aaaaattatg gtgatgatat tactctgaac
    11761 agagataccg ccgtggctac ggtgcagatt actgtcagca gaaaatgcat tactcaggaa
    11821 gaaatggatg aattatatgc attgtctgag agactgaaag aaaagtgcgc cggtaattaa
    11881 attatatcac tgtgccggga gatgatatga gccggaaaat atttgaactt tttgccctga
    11941 cagcaggact gtttatttct gcccacgctt cggaaaatgt gaatacaccc gaaaaccgcc
    12001 agttcctgaa gcagcaggaa acactgagtc agcagttacg tgaaaaaccg gacacacagc
    12061 tgcaggcatg ggcagaacag caggtcaggg cgaatcccct tcagcattcc gataaccagt
    12121 ttctcgatga actggtccgt aaacagcagg ctgctcagaa ggataaacca cagcagggtg
    12181 ccgtgtattt tgtgtcgttt tccatacccg aagaggggct gaaacggatg ctgggagaaa
    12241 cccggcacta cggtattccg gccacactgc ggggtatggt gaacaacgac ctgaaagcca
    12301 cggctgaagc agtgctgtct ctggtgaaag acggtgcgac cgatggtgtt cagattgacc
    12361 cgacgctgtt ttcacagtac ggcattcgca gcgtgccggc tctggtggtg ttctgcagcc
    12421 aggggcacga catcatccgg ggaaatctgc gcgtcagaca ggcgctggag aaagtggccg
    12481 ccacaggaga ctgccggcag gtggctcagg gactgctgga cggcaccggg gataagtcgc
    12541 aatgagttta cctgcaggct attaccggat tgacccggac atccgtgcac tggtggccgc
    12601 gatgaacgtt cacggatttc gtacatatgc gtcctgtcag gggcatggtt tccctgttac
    12661 aaagctgccg ccttatatcg cctttgcctg tccggtaaaa atggctgccc ttctggagca
    12721 gcgactgcgg caggatgctg aatcagcaat accacgtctg acatggggat ggtcggtgaa
    12781 ggggactttt aacagtgatt ttcagctttg cttccggctt cagcctgaag gccctcatca
    12841 ctggtatcac cggtactgtc gccgctcact gcgtgcagac ttcaggacgc tggtccgatt
    12901 agtgaatccg taaatctgtt cccgttaagg aggcattgat ggcgctgaca aagaaacagc
    12961 gggaaaaact gcgtatgaaa ttcggtggcc gttgtgcgta ctgcggctgt gaactgccgg
    13021 agaaaggatg gcatgccgac catgttcagg cggtgctccg caagtcagag cagtgcatga
    13081 aggctgcaga aaagcgcatt ttcaggctga aatccaccgg tgatgttttc agaccggagg
    13141 ccgactgtcc ggaaaacctc gtcccggcct gtgcgccgtg caacctgctg aaaacaacat
    13201 attcgctgga gatgttcaga acgcaggtgt ccctgcaggt tgaacggggg cgaagaagca
    13261 gcgtgaattt caggaccgca gaacggttcg ggctgattca ggtggtgaac aaaccagtcg
    13321 tgttctggtt tgagcaatat cagagggaga gtgaggtatg aaacgtcttc tgccattgct
    13381 gctggggctg gcagtcagtg tggcacaggc tgacagcaac agtgattacc gggcgggttc
    13441 tgattttgcc cgtcagattc aggggcaggg tacaggcagt attcagggct ttaagccaca
    13501 ggagagtatc cccggctata acgctaatcc ggacgagaca aaatattacg gtggtgtgac
    13561 tgctggcggg gatggcggcc tgaaaaatga cggcaccacg gaatgggcga ccggtgaaac
    13621 cggaaaaact atcacagagt cattcatgaa caagccgaaa gacattctgt caccggatgc
    13681 accgttcatt cagaccggca gggatgtggt gaacagggct gacagcattg taggaaacac
    13741 aggccagcag tgtagtgcac agcagataaa ccgcagtgaa tttacaaact atacctgtga
    13801 acgggacacg atggtggagg agttctgcac acggactgcc aggatcaccg gagactggaa
    13861 atacacggat gaatatcgtg aggtgacgat accgcactca cagttccggt tcagtatgaa
    13921 cgggctgaaa ctggtgttca gtgtcacggc tccggtaacc ggtacagtgg aaagtgcatc
    13981 attgtctgta tacgccgctt ttttcttcct gaattctcgt tataccttca tgaattcaac
    14041 gttcaatgtt ggcctggtaa acgggcagtc gggtactttc cccttgtccg gggcgacagg
    14101 tttgcaggtg acacaggggc aggtactgac cggcagtggc tgtactgcta acggtaactg
    14161 tctttctggc gggaacggtg accgacaggt ttatgagaaa ctagccagcg gcgcgtccac
    14221 ctttacgctg aaactcagga tgaaggtccg tgataaagaa tgggttccac gggtggagtg
    14281 ggtggagagt tgtccgttta ataaagcgga tggcgtgctg aaaggtactg agtgttcaga
    14341 gccgggtggt acgaaaaccg gagtgatgga aggtaaaccc tggagcatca cacaggcatg
    14401 ctgggcatac cgggataaat atgtcactca gtccgcagat aacggcacct gtcagaaata
    14461 tgtcgataat ccggcctgta cgctggcctc acgtcagtgc gcgttttatt ccgatgaggg
    14521 aacctgtctg catgagtatg ccacgtattc ctgcgagtca aaaacatcag gaaaagtgat
    14581 ggtctgtggt ggtgatgtct tctgccttga cggtgaatgc gataaggcac agagcggaaa
    14641 aagcagtgat tttggtgagg cagtatcaca gctggctgcg cttgccgccg caggtaagga
    14701 tgttgccgcc ctgaatggtg tggatgtcag ggctttcacg ggcgaggcga aattctgccg
    14761 taaggccgca gccggcttca gtaactgctg taaggatggt ggctggggtc aggatgtggg
    14821 gctggcgaag tgcaacagtg aagaaaaggc actgggaaag gcaaaggaca acaaactgac
    14881 ggtgagtgtg ggcgagttct gttcgaaaaa ggtgctgggc gtctgcctgc agaaaaagcg
    14941 cagctactgc cagtttgatt cgaagctggc gcagatagtc cagcagcagg ggcgtaacgg
    15001 gcagttgcgt atcgggtttg gcagcgccaa aagtcctgac tgtcggggga ttacggttga
    15061 tgagctgcag aaaattcagt tcgacagact ggatttcact aacttctacg aagacctgat
    15121 gaataaccag aaaatcccgg acagtggcgt tctgacgcag aaagtgaaag agcagattgc
    15181 tgaccagctg aaacaggcag ggcagtaagg tatgggaaac aaccggggga aatgatgaag
    15241 gtcatattca cgtcgaaccg ttttattgat tttcttatcc ggcttttgat tacagcgatt
    15301 gtcatttccc cggtaattat ctggtcgtgg gatacggtaa aagaaaccac ggcaaacggg
    15361 atgcttgccg ctgctttcgt tatcctttac tccggtgtgc tcctgtttat tctgtatttt
    15421 tgtttttctg ccctgactga cctgcagaaa cctgatgaaa gaaaaaatga tgaaaggaat
    15481 gaagatgaat aaagcattac tgccactgtt actctgctgc tttatttttc cggcgtcagg
    15541 gaaagatgct ggctggcagt ggtataacga gaaaataaat ccgaaggaaa aagaaaataa
    15601 acctgtacct gcagcccccc gtcaggagcc ggatattatg cagaaactgg ccgcgctgca
    15661 gacggcaaca aagcgggcgc tgtacgaagc cattctgtat cccggtgtgg ataattttgt
    15721 gaaatatttc cggctgcaga attactggac tcagcaggcc gggcttttca ccatgagtgc
    15781 taaaaaggcc atgctggcac atcccgaact ggactataac ctgcagtaca gccattacaa
    15841 cggcacggta cggaaccagc tggcagcaga ccaggcgcag cagcgacagg cgattgccag
    15901 actggcagag cattacggca ttatgttttt ttaccggggg caggaaccca tcgacgggca
    15961 gctggcgcag gtcattaatg gcttcaggga tacctatggt ctgagtgtta ttcccgtttc
    16021 cgtggatggc gtgattaatc cgctgttgcc ggattcccgg actgaccagg ggcaggcgca
    16081 gcgcctcggc gtgaaatatt tcccggccat gatgctggtt gacccgaaac agggcagtgt
    16141 tcgtccgtta tcatacggtt ttatttcgca ggacgacctg gcaaaacagt tcctgaacgt
    16201 ttctgaagat tttaaaccga acttctgaat tacggatatt ttccgttcgt cagtaaattc
    16261 cattaattct gaacctggta ataattcacc atttattccg gtagtccctt atttaattct
    16321 ctgtatgaca ggagagtttt actgtgcata aatataaatt ctctttaccc cggcttgata
    16381 ttacgggaat gtgggtattt tccctgggtg tctggtttca tatcgtcgcc cgtcttgttt
    16441 acagcaaacc ctggatggct ttttttctgg cggagttgat tgcggccatc cttgtgctgt
    16501 tcggcgcata ccaggtgctg gatgcatgga ttgcccgtgt cagccgggaa gagcgtgagg
    16561 cactggaagc caggcaacag gccatgatgg cagagcagca ggagggcgga catgtctctc
    16621 actaaattac tgccggtcat tttgttgatg atggccacag gtgtgcaggc ctccacccgg
    16681 gatgaaattg aacgactctg gaatccgcag ggtatggccg cgcagcctgc acaaccggca
    16741 gcagacacgt cagccaggac agaaaagccg gctccccgct ggttccgcct cagcaacggc
    16801 aggcaggtta acctggccga ctggaaggtg gtgctgttta tgcaggggca ttgcccttac
    16861 tgccaccagt ttgacccggt actgaagcag ttggcacagc agtacggctt ttccgttttt
    16921 ccctacaccc tggatggtca gggggatacc gcctttccgg aagcattacc ggtgccaccg
    16981 gacgtgatgc agaccttctt cccgaatatc ccggtggcca caccgaccac cttcctggtc
    17041 aacgtcaaca cgcttgaggc attaccgctt ttacaggggg caacggatgc cgccggtttt
    17101 atggcgcgga tggataccgt tctgcagatg tacggaggaa aaaaaggtgc gaaataagca
    17161 ggtggtgtta ctcattgccg gaatatccgg catcgcgacg ggaataatcg tcagcctgaa
    17221 tatccccttt atccgtcagg ggctttttta tcccgccagc cctgtagaaa tagtcgtttc
    17281 gctgtgtctt accttttctg tttctgttgt gttttttgtg ggggcaattg tgggatggat
    17341 tttggtgtct gaaatatatt acagccgcat gaccggtctg aatgaatcat cagtaatatc
    17401 agaggaaact tataatgaca gcaaacaaaa agaatcagga attcaaaatc agaaaaatta
    17461 gacggaatat tgagtatagt ttcagggatt ctgacagata ttttgatttg tttattgttt
    17521 tccttgttgc cggaattgtt ttatgggcag ttatgcacgt tattttcgat gtctgtattg
    17581 attcatggat ggccgacccg aaattactta atttccagta tatgtggaat gttctgatga
    17641 aagtcattcc gtttacgtta tgggcgctgg cagcagggtt tcttgttaca ttttttctgt
    17701 ccccaatgtg cgaattaata ttcggaaata tcatgatatt cctgctgaaa cgccgtatgc
    17761 gccgggagaa cacattaagg gagggcagca ataatgcttc gcattaagcc tctttttatt
    17821 ctttgtgccg ccttactgac ggtgacgcct gcagcgtcag cggatgtgaa cagcgacatg
    17881 aatcagttct ttaacaagct gggcttcgcc tccaatacca cacagtcggg cgtctggcag
    17941 gggcaggctg ccggctatgc ttacggcggc tccctgtatg cccgtactca ggtcaaaaat
    18001 gttcagctga tttccatgac gctgccggat atcaatgccg gatgtggcgg catcgatgcc
    18061 tacctcggtt cgttcagttt tattaatggc gagcagctgc agcgttttgt taagcagatt
    18121 atgagcaatg ctgccggtta cttttttgac cttgccctgc agacaacggt gccggaaatc
    18181 aaaaccgcaa aagacttcct gcagaaaatg gcaagcgaca ttaacagtat gaacctcagt
    18241 tcctgccagg cggcgcaggg gattattggc gggcttttcc cccggacgca ggtgtcccag
    18301 caaaaagtct gtcaggacat tgccggtgag agcaatattt ttgctgactg ggcggcttcc
    18361 cggcagggat gtaccgttgg cgggaaatct gacagtgtca gggataaagc cagcgacaag
    18421 gataaggagc gggtgaccaa aaacatcaac atcatgtgga atgcgctttc caaaaacaga
    18481 atgtttgacg gcaacaaaga gctgaaagag tttgtgatga cgctgaccgg ctcactggtg
    18541 tttggtccca acggcgaaat cacaccgctg tcagccagaa ccactgaccg ctcaattatc
    18601 cgggccatga tggaaggcgg cacagcaaaa atttatcact gcaacgattc tgataaatgc
    18661 ctgaaagtgg tggcagacac accggtgacc atcagccggg ataatgcact gaagtctcag
    18721 attactaaac ttctggccag cattcagaac aaggcggtca gtgacacgcc tctggatgac
    18781 aaggaaaaag gctttatttc cagtaccacc atccccgtct tcaaatacct ggttgacccg
    18841 cagatgctcg gagtttccaa cagcatgatt taccagctga cggactatat cggttacgac
    18901 atcctgctgc agtacattca ggagctgata cagcaggccc gggcgatggt ggccacagga
    18961 aattatgacg aagcagttat cgaacatatt aacgacaaca tgaatgatgc cacccggcag
    19021 attgcggcgt ttcagtcaca ggtgcaggta cagcaggatg cgctgctggt tgtcgatcgt
    19081 cagatgagct acatgcgtca gcagctttcc gcccgcatgc tcagtcgtta ccagaacaac
    19141 tatcacttcg gagggagcac gctgtgaatg aagtttatgt gattgccggt ggtgagtggt
    19201 tgcggaataa cctgaacgcc attgccgcct ttatgggaac caggacgtgg gattccattg
    19261 aaaaaattgc gctcacattg tctgttctcg cggtggccgt gatgtgggta cagcggcaca
    19321 acgtgatgga tttgctgggc tgggtggccg tgtttgtgct tatcagcctg ctggttaatg
    19381 tccgcacatc ggtgcagatt attgataaca gtgacctggt caaagttcac cgggtggata
    19441 atgtaccggt cggactggcg atgccacttt cactgacgac ccgtatcggg catgcgatgg
    19501 tggcaggtta tgagatggtg ttcgctcagc ctgacagcgc cacctacagc aagacaggca
    19561 tgctgttcgg ggcgaacctg attgtgaaaa gcaccgattt cctgtcccgg aatccggaaa
    19621 tcatcaatct gtttcaggac tacgtccaga actgcgtgct gggtgatatt tacctgaacc
    19681 acaaatacac gctggaagac ctgatggcct ccggtgaccc ctacacgctg attttttccc
    19741 ggcccagccc gctacggggc gtttatgaca gcaataataa tttcataacc tgtaaagatg
    19801 cgtcggtcac gctgaaagac aggctgaatc tcgatacaaa gacgggaggc aagacctggc
    19861 attattatgt gcagcagata tttggcggca ggccggaccc tgacctgtta ttcagacaac
    19921 tggtgagtga cagttacagt tatttctacg gctccagcca gtctgccagc cagattatgc
    19981 gccagaacgt caccatgaat gccctgaaag agggtatcac cagtaatgcg gcccgtaacg
    20041 gtgataccgc cagcctggtc agtctggcca cgacttcgtc gatggagaaa cagcgtctgg
    20101 cacatgtctc cattggtcat gtgctgatgc gaaacctgcc gatggtgcag accattctgg
    20161 tgggcattac cattggtatc ttcccgctcc tggtactggc ggctgtcttc aacagactga
    20221 cgctgtctgt tctgagaggc tatgtgtttg ctctgatgtg gttccagacc tggccgttgc
    20281 tgtatgccat tctgaacagt gccatgacat tctatgcaaa gcagaatggt gcaccggtcg
    20341 tgctctctga actctctcag atacagctga aatactccga cctggcgtcc actgccggat
    20401 atatatctgt catgataccc ccgttgtcat ggatgatggt aaaaggcctt ggcgcaggtt
    20461 tttccagcgt gtacagccat ttcgcttcct ctgctatcag tccgactgcc agtgcggcag
    20521 gtagtgtggt tgacggtaat tactcctacg gcaacatgca gacggaaaac gtgaacggct
    20581 tcagctggag caccaacagc accacgtcgt ttggtcagat gatgtaccag accggcagtg
    20641 gcgcaaccgc cacacagacc cgtgacggta atatggtgat ggatgcaagc ggagctatgt
    20701 cccggttacc ggtcggcatc aatgcaacgc gtcagattgc ggcagcacaa caggaaatgt
    20761 cccgggaagc atcgaacaga gcagaaagtg ccctgcatgg gttcagcagc agtattgcca
    20821 gtgcctggaa cacgctcagt cagtttggtt ctaaccgggg gagcagtgat tctgtcacag
    20881 gtggtgctga cagcaccatg agcgcacagg actccatgat ggccagccgt atgcgcagtg
    20941 cagtggaaag ctatgcgaag gcgcataata tcagtaatga gcaggcgaca agagaactgg
    21001 catcgagaag tacaaatgcg tcgcttggcc tttacggtga tgcttatgct aaagggcatt
    21061 taggcataag tgtattgggt aacggtggag gggttggtct ccaggccgga gctaaggcaa
    21121 gcattgttgg cagtgatctt gactcacatg aagccagtag tggttcacgg gccagccatg
    21181 atgctcgtca tgatatcgat gccagagcta cacaagactt taaagaggcc agcgattact
    21241 ttaccagccg caaagtcagt gaatccggca gccatactga caataatgcc gattcccgcg
    21301 tggaccagct gtctgcagcc ctgaactcag cgaaacagag ttacgaccag tacacgacga
    21361 atatgacccg cagccatgaa tatgctgaaa tggcatcccg tactgaaagc atgagtgggc
    21421 agatgagtga agatctgtcg caacagtttg cacagtatgt gatgaaacac gcaccgcagg
    21481 atgccgaagc tattctgaca aacaccagtt caccggagat tgcagaacgc cgccgggcga
    21541 tggcgtggtc ttttgtgcag gaacaggtgc agcctggtgt tgataatgcc tggcgtgaat
    21601 cccgtggcga tattggtaaa ggaatggaga gcgtaccttc gggtggtggc agccaggata
    21661 ttattgctga ccatcagggg catcaggcca ttattgatca aagaacgcag gacagtaata
    21721 tccggaatga tgtaaaacat caggttgata atatggtcac agaatataaa gggaatatag
    21781 gtgatactca gaacagtatc cgtggcgagg aaaatattgt aagagggcag tattctgaac
    21841 tacaaaatca tcataaaaca gaagcattaa ctcagaataa taaatataat gaagaaaagc
    21901 tggctcagga aagaatacct ggtgctgata gtccgaaaga gcttttagag aaagcaaaga
    21961 gttaccagca taaagaataa tcgtaacatg ttgggtaggg tatggagagg ccatgattac
    22021 acaacaaatt atcagtagtg agttggaagt tttaaagaaa catattgact caggagatat
    22081 tagaattccg tcactatggc agggattaaa acccgggttt ataatcatgg gatggatgat
    22141 tttttgtccc ttactaatgt catttctaat aacacaaaag acaagtgaaa cactcactgc
    22201 tgtgttagcc ggaggatggc ttggacttat tattctgttt attgtggcaa ggattagaat
    22261 gttatatttc tctctgccgg aagaattcct aaagacatca tccgtaatga ggattatatc
    22321 atcaaaactg aaagtgtatt tcattgtgta tatgggaatt atatttttat ggtctttttt
    22381 aggtggaggg attatatacg gttttggtgc aattttagtg acggttatta tggcgttctt
    22441 gattcaatta gatatcggta gatatcaatt tgttggtgtt attgatgcaa tcaatagtta
    22501 cgtcaaaaac aagaagttat caagagtaaa ataaaagata ttagagagta aatatatgat
    22561 gaaaacaaaa aaactaatga tggttgcact ggtcagttcc actctggtcc tttcagggtg
    22621 tggtgcgatg agcacagcaa tcaagaagcg taaccttgag gtgaagactc agatgagtga
    22681 gaccatctgg cttgaacccg ccagcgaacg cacggtattt ctgcagatca aaaacacgtc
    22741 tgataaagac atgagtgggc tgcagggcaa aattgctgat gctgtgaaag caaaaggata
    22801 tcaggtggtg acttctccgg ataaagccta ctactggatt caggcgaatg tgctgaaggc
    22861 cgataagatg gatctgcggg aatctcaggg atggctgaac cgtggttatg aaggcgcagc
    22921 agttggtgca gcgttaggtg ccggtatcac cggatataat tccagttctg ccggtgccac
    22981 actcggtgtg ggccttgctg ccggtctggt gggtatggct gcagatgcga tggtggaaga
    23041 tgtgaactat accatgatca cggatgtgca gattgcagag cgtactaagg caacggtgac
    23101 aacggataat gttgccgccc tgcgtcaggg cacatcaggt gcgaaaattc agaccagtac
    23161 tgaaacaggt aaccagcata aataccagac ccgtgtggtc tcaaatgcga acaaggttaa
    23221 cctgaaattt gaagaggcga agcctgttct cgaagaccag ctggccaaat caatcgcaaa
    23281 tattctctga acctcgtcag gaggctggtc tgaccggcct ccttgctgac gcctcgcgtt
    23341 gctcgttgtc caaccccggg acgtaacaaa ataaattttg tcacttcccg acgtttccga
    23401 aataaatatg aaaacactga gcggttgtgc aaattctttg tgcgaccgtg aagtgttgcc
    23461 ggcgaagccg ggatcttccg gagaaagtca ttatgacatc agacaatatc gctgaatatg
    23521 ttggtgagaa taatcaattt actgagaagc ctgtaactga ataccccccg gtgatacaga
    23581 ataatacagt gaccactaaa gactatctgt acagcaataa tattgatact gaccttttag
    23641 tggttatcgg agggcttatt gttatattgt ttctcatgat gcttatcatg tactttatta
    23701 attctctcag gcataaggaa ttaactgaaa tactgacaaa aatatctttt cctgaaaaat
    23761 ccgggagcaa ttcgcttaaa ccggaagcgg tcagctgtaa tttgttgtcc ctgtgtgtgc
    23821 tggcaccggt attctttaca cccagaaagc tggctgaagt ttccagggag atcatcgtgg
    23881 cgagtgcgca ggaaattgca tggacgccgg ggagcagtcg tattgatggc caccaggcgg
    23941 agaatatcac ctgggcgatt tcatcgcagt ctcctgccga taagtatgat ctcatcaata
    24001 aaacgtcccg tgtttctcct gacagtggcc gtttttatga accggacaca gttctggatc
    24061 accgtgagcg ggtacgcctt tttgttcaga tccagtttcc gcatgggagt gactggtctg
    24121 aagtattgcg cctgactgac attgttacag agaaggtctc ttccggcagg gctgcaggcg
    24181 ggtatcacca ccggcataca gggtacacct ggtctgtttc tgtgaattaa ccccacatca
    24241 tccttttctg ccctgttcca gattttcccg gagtttcatc atgagtttta acgcaaagga
    24301 tatgacccag ggcggtcaga ttgcgtccat gcgtatccgc atgttcagcc agatcgccaa
    24361 tatcatgctt tactgcctgt ttattttttt ctggatactc gttggtctgg ttttatgggt
    24421 aaaaataagc tggcagacgt ttgtgaacgg ctgtatttac tggtggtgta ccacgctgga
    24481 aggcatgcgg gatttaatca agtcccagcc ggtctatgag atccagtatt acggcaaaac
    24541 cttccggatg aatgctgctc aggtactgca tgataaatat atgatctggt gcggagagca
    24601 gctatggtcc gcattcgttc tggccacagt tgtggcactg gttatttgcc tgattacctt
    24661 ctttgttgtc tcctggattc tggggcgtca gggtaaacaa cagagcgaaa atgaagtcac
    24721 aggtggtcgt cagctgacag acaatccgaa agacgttgcc cggatgctga aaaaagacgg
    24781 caaggactcc gatatccgga ttggcgacct gccgattatc cgggattctg aaatccagaa
    24841 cttctgtctg cacggcacgg tcggggccgg taagtcggaa gttatccgtc gtctggccaa
    24901 ctacgcccgt cagcgcggtg atatggtggt gatttatgac cgttcagggg aatttgttaa
    24961 aagttactat gatccctcca tcgataagat cctgaatccg ctggatgccc gctgtgccgc
    25021 ctgggattta tggaaggagt gtctgacaca gccggatttt gataatacgg caaataccct
    25081 gatcccgatg ggcacaaaag aggacccgtt ctggcagggt tcaggacgta ccatttttgc
    25141 ggaagcggcg tacctgatgc gtaatgaccc caaccgcagc tacagcaaac tggtggacac
    25201 actgctttcc atcaaaatcg aaaaactgcg taccttcctg cgtaattcac cggcggccaa
    25261 cctggtggaa gagaaaattg agaaaacggc gatttccatc cgtgctgtgc tgaccaacta
    25321 cgtgaaagcc atccgttacc tgcaggggat tgagcataac ggtgagtctt ttaccatccg
    25381 tgactggatg cggggtgtcc gggaagatca gaaaaacggc tggctgttta tctcgtcgaa
    25441 tgccgacacc catgcctccc tgaagccggt gatctccatg tggctgtcca ttgccattcg
    25501 tggtctgctg gcaatgggag aaaaccgtaa ccgtcgtgtg tggtttttct gtgatgagct
    25561 acccacgtta cacaaactgc cggacctggt ggagatcctg ccggaagccc gtaagttcgg
    25621 tggctgttat gtgtttggta tccagtccta tgcccagctg gaagatatct acggtgagaa
    25681 agcggctgcc acgctgtttg acgtcatgaa cacccgtgcc tttttccgtt ctcccagcca
    25741 taagattgca gagttcgctg ccggtgagat tggtgagaaa gagcacctga aagccagcga
    25801 gcagtactcc tacggtgctg atccggtacg tgacggggta tcgaccggta aggatatgga
    25861 gcgccagacg ctggtcagtt attccgacat tcagtctctg ccggatttga cctgttatgt
    25921 caccctgccc ggaccgtatc cggcagtaaa actctcactg aaatatcagg cacgtccgaa
    25981 ggttgcccct gagtttattc cgcgtgacat caacccggaa atggagaacc gcctgagcgc
    26041 cgtacttgcc gcaagggaag cagaaggtcg tcagatggcc agcctcttcg aaccggatgt
    26101 gccggaagtt gtttccggag aagacgtgac tcaggctgaa cagccgcaac agccgcaaca
    26161 gccgcaacag ccgcaacagc cgcaacagcc gcaacagccg caacagccgc aacagccgca
    26221 acagccggtg tctcccgcca tcaacgataa gaagtcagat tcaggtgtga atgttccggc
    26281 aggggggatc gagcaggagc tgaaaatgaa accggaagaa gagatggaac agcaactgcc
    26341 acccgggatc agtgaatccg gtgaagtggt ggatatggcc gcttatgagg catggcaaca
    26401 ggaaaatcat ccggacatcc agcagcagat gcagcgtcgt gaagaggtga acattaatgt
    26461 gcaccgggag cgcggggagg atgttgagcc gggagatgat ttctgatgat gagtatcgcg
    26521 caggtcagat cggccggaag tgccggtaac tattataccg acaaggataa ttactatgtg
    26581 ctgggcagca tgggagaacg ctgggccggc aggggggctg aacagctggg actgcagggc
    26641 agtgtcgata aggatgtttt tacccgtctt ctggagggaa ggctgccgga cggagcggat
    26701 ctaagccgca tgcaggatgg cagtaacagg catcgtcccg gctacgacct gaccttctcc
    26761 gcccccaaaa gtgtctccat gatggccatg ttaggtggcg ataagcgcct gattgatgca
    26821 cataaccagg ccgtggactt tgctgttcgt caggtggagg cgctggcctc cacacgggtg
    26881 atgacggacg gacagtcaga aacggtgctg accggtaatc tggtgatggc actgtttaac
    26941 cacgacacta gtcgcgatca ggaaccacag ttacacacgc atgcggtggt ggctaatgtc
    27001 acgcagcata acggtgagtg gaagacactg agcagtgaca aagtggggaa aacggggttc
    27061 attgagaatg tgtacgctaa tcagattgcc tttggcaggc tctatcggga aaaactgaaa
    27121 gagcaggttg aggcgctggg ctatgaaacc gaagtggtgg gtaagcacgg tatgtgggag
    27181 atgccgggcg taccggtgga ggccttttcc ggacgctcac aggctatccg agaggccgtc
    27241 ggggaagatg cctcgctaaa atcccgggat gtggcggcac tggatacgcg aaaatccaaa
    27301 cagcacgtcg atcctgaagt cagaatggct gaatggatgc agacgctgaa ggaaaccggg
    27361 ttcgacatcc gggcatatcg tgacgcggcg gatcagcgtg cggagacccg cacgcaggcg
    27421 cccggggctg tttcacagga ggggccggat gtgcagcagg cggtgacaca ggcgattgcc
    27481 ggattaagtg aacgaaaagt gcagtttacg tacacggacg tgctggccag aacagtcggc
    27541 atactgccgc cggaaaatgg tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc
    27601 agccgtgagc aactcatccc ccttgaccgt gagaaggggc tgttcacgtc cgggattcat
    27661 gtgctcgatg aactgtcagt ccgggcactc agtcgcgata tcatgaaaca gaaccgggtg
    27721 accgtacatc cggagaaaag tgtcccccgg acggccggct acagtgatgc cgtcagtgtt
    27781 ctggcacagg accgtccgtc gctggccatt gtgtccgggc agggcggtgc agccgggcag
    27841 cgtgagcggg tggctgaact ggtcatgatg gcccgggagc aggggcggga ggtgcagatt
    27901 atcgctgctg accgtcgctc gcagatgaac ctgaagcagg atgaacggct gtccggtgag
    27961 ctgataaccg gacgtcgtca gctgccggaa ggcatggcct tcacgccggg cagtactgtt
    28021 atcgttgacc agggcgaaaa actctccctg aaagagacgt taaccctgct agacggtgcc
    28081 gcacgtcata acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaggcagt
    28141 gcgctgatgg ccatgaagga tgccggggtt aacatatatc gctggcaggg cggagagcaa
    28201 cgaccggcaa ccatcatcag tgaaccggac cgtaatgtcc gctatgcccg gctggccggg
    28261 gactttgcgg ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggggtacgg
    28321 gaacaggcca tactgacaca ggccattcgc agtgagctga aaacacaggg cgtgctcgga
    28381 cacccggagg taaccatgac cgccctgtca ccggtctggc tggacagccg gagccgttat
    28441 ctgcgggata tgtaccgccc gggaatggtg atggagcagt ggaacccgga gacacgcagt
    28501 catgaccgct atgttatcga ccgggtgacg gcgcagagtc acagcctgac cctgcgggat
    28561 gtgcagggcg aaacgcaggt ggtgcgtatt tcctccctgg acagcagctg gtcgctgttc
    28621 cggccggaaa aaatgccggt ggcagacggc gagcgactga gggtgaccgg gaaaattccc
    28681 ggactccgag tctccggcgg tgaccgcctg caggtggcat ccgtcagtga agatgtgatg
    28741 acggttgttg tgccggggcg ggctgaaccg gcctccctgc ctgtgagcga ttcaccgttc
    28801 acggcactga agctggagaa tggctgggtg gaaacgcccg ggcattccgt cagcgacagt
    28861 gcgaaggttt ttgcctccgt cacacagatg gcaatggaca aggccaccct gaacggtctg
    28921 gcccgcagcg gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa
    28981 aaacttgccc gccatccgtc ctttacggtg gtttctgagc agataaaggc gcgggccggt
    29041 gagacatcgc tggaaacggc tatcagtcat cagaagtccg cacttaacac gccggcacag
    29101 caggccattc atcttgccct tccggtggtg gaaagtaaaa acctggcctt cagccatgtg
    29161 gacctgctga cagaggcaaa gtcgtttgct gcagaaggaa ccagttttgc tgacctgggg
    29221 cgggaaatcg atgcgcagat aaaacggggc gatttactgc atgtggatgt ggcaaaaggt
    29281 tatggcacag acctgctggt ttcccgtgcg tcgtatgagg ctgaaaaaag cattcttcgc
    29341 catattctcg aaggtaagga ggcggtcacg ccgctgatgg agagagtacc tggcgaactc
    29401 atggagaaac tgacatcagg acagcgcgcc gccacccgca tgatactgga aacgcccgac
    29461 cgtttcacgg tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg
    29521 atgtcagccg tgaacatgct gccggagagt gagcgtcccc gtgtcgttgg gctgggcccc
    29581 acgcaccgtg cggtcgggga gatgcgcagc gccggcgtgg atgcacagac actggcgtcc
    29641 tttctgcatg acacgcaact gcagcagcgc agcggagaaa cgccggattt cagcaacacg
    29701 ctgttcctgc tcgatgagag ctcaatggtg ggcaataccg acatggcacg ggcatacgcc
    29761 ctgattgcgg ccggtggtgg tcgtgccgtg gccagcggtg acacggacca gctgcaggcc
    29821 atcgcgcccg gtcagccttt ccgtctccag cagacgcgca gtgctgccga tgtggccatc
    29881 atgaaggaga ttgtgcgtca gacgccggaa ctgcgggagg cggtatacag cctgattaac
    29941 cgggatgtgg aaagggcact gtccgggctt gagagtgtga aaccgtctca ggtgccacgt
    30001 caggagggcg catgggcacc ggagcactcc gtgacggagt tcagtcacag ccaggaagca
    30061 aaactggcag aagcgcagca gaaggcgatg ctgaaaggcg aggctttccc ggatgtcccc
    30121 atgacactgt atgaagccat tgtccgcgac tataccggca ggacaccgga agcacgggag
    30181 cagacgctga ttgtcacgca cctgaatgag gaccggcgcg tgctgaacag catgattcat
    30241 gatgcacggg aaaaggccgg tgagctggga aaagagcagg tcatggtgcc tgtcctgaac
    30301 acagcgaata tacgtgacgg agaactgcgt cgtctttcca cctgggagac acatcgggac
    30361 gcacttgccc tggtggataa tgtgtatcac cggattgccg gtatcagcag ggatgacggg
    30421 ctgataaccc tgcaggatgc ggaaggtaac acgcggctga tttcgccccg ggaggcggtg
    30481 gctgaaggtg tcacactgta caccccggac aaaatccggg tggggaccgg tgaccggatg
    30541 cgcttcacga agagtgaccg ggagcgtggt tatgtggcca acagcgtctg gacggtgaca
    30601 gcggtatccg gtgacagtgt cacgctgtcg gacggacagc agacccgggt gattcgcccc
    30661 gcccaggagc aggcagagca acatattgac ctggcctatg ccatcaccgc ccacggtgcg
    30721 cagggtgcga gtgaaacctt tgccattgcg cttgaaggca cggaaggtaa ccggaaactg
    30781 atggccggct ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg
    30841 tacaccgata accgtcaggg ctggacggat gccattaaca atgccgtaca gaaaggaact
    30901 gcccacgatg tgctggagcc gaaaccggac cgggaggtca tgaatgcaga gcggctgttt
    30961 agtacggcgc gggagctgcg ggacgtggcg gcagggcgtg ccgttcttcg tcaggcgggg
    31021 cttgccgggg gagacagtcc tgcacggttt attgcaccgg ggcgtaaata tccacagccg
    31081 tatgtggcac tgccggcgtt tgaccgtaac ggcaaatcag ccggtatctg gctgaacccg
    31141 ctgacctcgg atgatggaaa cgggctgcgg ggattcagtg gtgaaggccg tgtgaaaggc
    31201 agcggggatg cgcagttcgt ggccctgcag ggcagccgta acggagaaag cctgctggct
    31261 gataatatgc aggatggtgt ccggattgcc cgtgataatc ctgacagtgg tgtggtggta
    31321 agaatcgccg gtgaaggccg tccgtggaat cccggtgcca tcaccggtgg ccgcgtgtgg
    31381 ggggatatcc cggacaacag cgtccagccg ggagccggaa atggcgagcc ggtcacggca
    31441 gaggtactgg cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgccgtgcg
    31501 gatgaaattg tccgtaaaat ggcagagaac aaacctgacc tgccggatgg caaaacagag
    31561 caggctgtca gggagattgc cgggcaggtg cgtgagcggg ctgccataac tgaacgggaa
    31621 actgcgctgc cggagagtgt gctgcgtgaa ccacaacggg agcgggaagc ggtccgggag
    31681 gttgcccggg aaaatctgct gcaggagcgt ctgcagcaga tggagcggga tatggtccgt
    31741 gacctgcaga aagagaaaac cctgggcgga gactgataca ggaaggcaaa cgctgatgac
    31801 aacggataac acgaacacga cacgtaacga ttcactggct gcccggaccg atacctggtt
    31861 gcagtcgttg ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt
    31921 gctgatggta ctggaccaca tcaatctgat attccagctg aagcaggaat ggatgtttct
    31981 ggccgggcgt ggagcctttc cgctgtttgc cctggtgtgg gggctgaatc tgtcccgtca
    32041 tgcgcatatc cggcaaccgg ccattaaccg gctgtgggga tgggggatta ttgcccagtt
    32101 cgcatattat ctggcaggct ttccctggta tgaggggaat atcctgtttg cctttgcagt
    32161 ggcggcacag gtgctgacgt ggtgtgaaac gcgcagtggg tggcgtacag cagcggccat
    32221 tcttctgatg gcgctgtggg ggcctttgtc cggcaccagt tacggcattg ccgggctgct
    32281 gatgctggca gtcagccacc ggctgtaccg ggcggaagac agagcggaac gtctggcgct
    32341 ggtcgcctgc ctgctggctg ttattccggc gcttaacctt gccaccagtg atgcagcggc
    32401 ggtggccggt cttgtgatga cggtgctgac cgttggtctg gtgtcgtgtg caggaaaatc
    32461 attaccccgt ttctggcccg gggatttttt cccggtattc tacgcctgtc atctggctgt
    32521 gctgggcgtt ctggcgctgt gacgggtgtg gtatctttga ccgcaagagg atgattcgtc
    32581 agaggcagaa cacagcatga cagagcagaa gcgaccggta ctgacactga agcggaaaac
    32641 ggaaggggag acgcctgtcc gcaggcggaa aaccatcatc aatgtcacca cgccaccaaa
    32701 gtggaaggtg aaaaagcaga agctggccga gaaagcagcc cgggaagcag agctggcggc
    32761 aaaaaaagcg caggccagac aggcactgtc catttatctg aatctgccca cgctggatga
    32821 ggccgtgaat accctgaaac cctggtggcc gggattgttt gacggtgaca cgccccggct
    32881 tctggcctgc ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct
    32941 ctcgcataaa aaactgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg
    33001 tgccatgaaa gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc
    33061 tcaggaggag gaagcgtatg cggcagcgcg tctggataaa atccgccgcc agaaccggat
    33121 aaaggcagaa cttcaggccg tgcttgatga gaaataaaaa aaagcctcca tcagagagga
    33181 ggcagggaaa taaggcttaa aaggaaataa cttccgtacc cgaaaggaag ccaattcagt
    33241 agattattta acaccataat actgacgggt taaagaggag tgataaagat gaatggattc
    33301 agaaacagtt cacggaacgg tcaggtctgg cgctggcagc gtgccggaag ccgtgccgtc
    33361 agcctggagg tcaggggatt ccggaaggag gccgctgaag cgtggctcct ggctgcccgt
    33421 gttgctcccc gtacagactg gcagcagttt gcacggcaga gagccgaacg ctgtcagcga
    33481 cggagataat gaaaaaaaac ggcaaaaaaa agacttcccc tgagcaaagg aggccaaaaa
    33541 tcattcatag cgtattactg acattgaatg aatgctctgt acatcaaccc tgtgaagcta
    33601 ccaccggtta atgccgggag gttgcgggaa gaaagataat acacagtcat tacgtggtta
    33661 atatctgtca gaatgttttt tgtgaccata aactgtcaga tggataaaaa agaccgtcat
    33721 gtgacggtca gtgttgtcct gtcagggaca aatgcattca gtaaggagag caaaataata
    33781 acatcacgtt gcccggctgt taatatcagg cgatgttaag tgttatttat gactctttta
    33841 tctggttgtg aagtctgtta atcaggaaga gaaaatggcg accggaaaat cagagaagca
    33901 acaggtgatt gcctttatcg attacgagaa ctgcagtaac ctgacagaaa tttccttaaa
    33961 tatatatacg gagttaatta tattcgcggg tgcgaaacag aataatgtgg cactgccagt
    34021 aaacgctttc cctgaagcag taaaaatcac actccggcat gtcagtgaag tctcccggaa
    34081 caatgttgat tttcatctgg tgctggagct ggggcagtgt gtatgccact acgggccgga
    34141 gaaggaatac catattgttt ccgctgacaa gggatatgac ggtattgtcc ggacactgca
    34201 ggcgcgcggg attcgctgcc gtcgcgtctc cccggataag gcgtcatcag taaagatggc
    34261 tgcacccgac atggatatcg ttatccggtg ggcaaacaaa attcaacagg cagcgcggga
    34321 ggttcgtaat atgccggtaa cagaaaagac atttaataat tatttaaaat cacagatgcg
    34381 cggtgaatgg cgtgatgtcc tgaccagagg tatccgggat gaactggtcc gtcgcggggt
    34441 aatgaaaatt caggggaata aaatcatctg gtaagactgc cagaataaaa tgatatttat
    34501 tttatttata aaaacagaaa ggtcgaaaat caataatggc atttatttcc atgacaagac
    34561 agaaagcgcc gtacagatat gcaagcaaga cccggttatc gataagacga acagactcag
    34621 gctctgccgg gtgttatgtg acggttgccg ttcgggggaa gcaggaattt attattgctg
    34681 aacttgattc agaaatccgg aaaatcaggc tgaaactgac ggactcctat gaagaagggg
    34741 taaggctgtc ttcgggcacg tttacgcttc cggccagatt ctgccgggaa atactgccgg
    34801 acgacgtcag gagtatcacc attattctgg aaaaaagcga cgatgagtgg tggtacggga
    34861 gttactgata tgtggcagaa ctccggacac tgacgccttc gcgttgctca gttgtccaac
    34921 cccggaaacg ggaaaaagca agttttcccc gctcccggcg tttctgcaat aaaaagcagg
    34981 ggcatggagg tcgtcgtaac cagacaggaa tgtggtatta tcgcggcggg tgtctgagcc
    35041 tttctggttc aggcaagacg caggtaccag aaatgcgaag accccactcg ttaatccatt
    35101 aactcgtgag gtctgcatga agtacttagc actactgatt gtagcctctt ctttgcagag
    35161 aggtcaaagt ttatgacgaa atatgccctt atcgggttgc tcgccgtgtg tgccacggta
    35221 ttgtgttttt cactgatatt cagggaacgg ttatgtgaac tgaatattca caggggaaat
    35281 acagtggtgc aggtaactct ggcctacgaa gcacggaagt aagctgccgg gcggggacgg
    35341 aagtccccgc tttccggaag tgtgaggtat ttcaggggca gacacccgac atgccagaaa
    35401 cagccggtcc cgcccggggc cggcatccag gttaaggcat ttcctgtttt tcagtcattt
    35461 catcatcaaa atcacattaa acggtcgtaa tcagacatga tttgtgcgcc aacacagatc
    35521 atcatcacaa ctttcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
    35581 cctgtttgag aattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatc
    35641 aggtacaaag cgagcatacc gaaaaggtaa tcctctgacg cttgcagaac gtcagcaggc
    35701 atctctggca agaaaacgtg ccacacataa agaactcaga gtgttcattc ctgctgcgtt
    35761 aaaagcgcaa ttacaggaga tgtgtgacgc tgaaggtgtt actcaggctg agatgattgc
    35821 tgaactgata aagcagaaga gtgctttcag ctaactaaaa tgtagcgaca tcacattctt
    35881 gcctgtttgt ggtgtgcgtg atagattgct gatcgtttaa ggaattttgt ggctggccac
    35941 gccataaggt ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcgaaaa
    36001 ccccgataat cttctttaat tttggcgagt cagaaagatt accggggcta acaagaaact
    36061 gcatagaagc tgttgctcta tgcggggagt atagttatat gcccggaaaa gttcaagact
    36121 tctttctgtg ctcactcctt ctgtgcaaca taagtgcagg atggt
//
