LOCUS       NZ_CP115736             5310 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed9, complete
            sequence.
ACCESSION   NZ_CP115736
VERSION     NZ_CP115736.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                :: C_braakii_GW-Imi-1b1
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/09/2024 02:21:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115736.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed9"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            complement(join(4968..5310,1..668))
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26230"
     CDS             complement(join(4968..5310,1..668))
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_097455288.1"
                     /translation="MASYHLTVKSGAKGKGASHAAYISREDKYAKKEKYEDLENKQHGN
                     MPSWAKDNPNLLWQAADNFERANGTSYREFEVALPRELTPDQRLELVNDFVKQEIGDRH
                     AYQFAIHCPNAGIDGKEQPHAHIMFCERINDGIDRDPEHFFKRANSKNPERGGAKKASE
                     PQTHTQRKEALVALRERWAEIQNKHLEKHGCADRVDHRSLKEQGIEREPEKHLGPKFVS
                     SMTVEQASAILERRVLERELLRENNARDSIIDLSTDVRSAIAEKDKSTNIEQEMRAGVE
                     AFKNAFEGKKAFMSGFEAFKKEQEIAREKEVKKSSQRNISRGGRGLDRGNDGYER"
                     /name="MobA/MobL family protein"
     gene            837..1304
                     /gene="traD"
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26235"
     CDS             837..1304
                     /gene="traD"
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_064645523.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     gene            2914..3528
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26245"
     CDS             2914..3528
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487403.1"
                     /translation="MNKGRFILGFSIYTALQAFSVGAMAGNQLWVTTDRTDRHTCPSTE
                     CGVAGRLMFREGVEALEKKGKWVRITKTYSASCVGGKSEYVRDGNKSCTSANGINDGKF
                     SEWVQSKDLSEKRPEDPAEGASGDDVLVKGSDDYGIYKTQFAKAARQLITDGICTEGDF
                     QEMGGWMASTSQGSGMYFTYCGGMTLSNKVYLDAKSGQTSR"
                     /name="hypothetical protein"
     gene            complement(3640..4026)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26250"
     CDS             complement(3640..4026)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265422890.1"
                     /translation="MNIGKSISLVGIAAVVTAIVAAVDATAIYYFHLNVLTTLVVTIFF
                     VSLYFVFAVSDAERRSGSNFAARKVKEAESELHGLLWDEMDGDARMKWFEKVGTNAIPN
                     ELKFKYERIQERVRYWEKEQNRLS"
                     /name="hypothetical protein"
     gene            complement(4442..4987)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26255"
     CDS             complement(4442..4987)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487382.1"
                     /translation="MMVMNAEERLYGMMAVVEEQQKVLSTSLAEFKKVTEELRTQKNEL
                     SKTVDSVSSEVSKAVSSSVIQDIGASQKAFNNEMMASCSQSLKVAKDIAKDLNQAKESS
                     KDIYNVINKTLTKEVGKTVTIFTAVFLIISICFIGGFAWYYATKAMEQKEVYEYWVQKS
                     DAAYSKCMKIKSCAKELK"
                     /name="hypothetical protein"
     Typing          2244..2355
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.088"
                     /coverage="99.0"
                     /evalue="9.88e-24"
ORIGIN
        1 gagctaacaa atttagggcc taaatgtttt tccggctcgc gctcgatgcc ttgctctttc
       61 agtgaccgat gatcaacacg gtcggcacat ccgtgttttt caagatgctt gttttggatt
      121 tccgcccaac gttcacgcaa cgcgacaagc gcctctttcc tctgcgtgtg cgtttgtggt
      181 tcgctcgctt ttttcgcgcc gcctcgttct ggattcttac tgtttgcacg tttgaaaaag
      241 tgttcaggat cgcgatctat accgtcgtta attcgctcgc aaaacatgat gtgcgcgtgt
      301 ggctgttctt taccatcaat ccccgcgtta gggcagtgaa tagcgaattg gtaagcatgg
      361 cgatcgccta tctcttgttt tacgaaatcg ttcaccaatt caaggcgctg atcgggtgtc
      421 aattctctgg gtaatgcaac ctcaaattca cggtaacttg ttccgttagc gcgttcgaaa
      481 ttatcagccg cttgccatag caaattcgga ttatccttcg cccatgaagg catattcccg
      541 tgttgcttgt tttctaggtc ttcgtacttt tcttttttgg catatttatc ttcgcgtgaa
      601 atataggcgg catgggaagc acccttcccc tttgcgccgc ttttcactgt cagatgataa
      661 gatgccatga tgttgacctt tagccctcgg caggagcgca cagtttctct aacgaagtga
      721 gaaactacta agtgcgcact gataataaaa agcttatcag tgattctacc tgcatttgac
      781 ctcggccgcc attcctgacc ataatttcca catcaacatt cagaggataa atgggcatga
      841 ataaaagcga aaaacaggaa aaatgggcag cagacagagt gcagtacatt cgcggcttaa
      901 agtcgccgaa tgaacagcag aaactcatgc tgatactgac ggataaagcg gataaaacag
      961 cgcaggatat caaaacgctg tccctgctga tgaaggctga acaggcagca gaaaaagcgc
     1021 aggaagccag agcaaaagtc atgaatctga tacaggccga aaaacgggca gaagccagag
     1081 ccgcccgtaa agcccgtgac catgcgctgt accagtcagc cggactgctt atcctggcgg
     1141 gtctggttga cagccagacg gggaagcctg ttgatgatac cgctgccttg ctgggtgcat
     1201 tagccagtct gaatgacctg tcgagggata atccgaagtg gtcagactgg aaaatcagag
     1261 gtcaggaact gctgaacagc aagaagtctg acagctctgc atagcggggt ctgaaggcca
     1321 atagaacgaa aacgtacgtt agtgaagtaa ctgtctgata tatcgaaaca taatgtacat
     1381 tggaaaacgc catcaaaacg gtgtcttttt aatcgaaaat tggcacttaa cggactttct
     1441 tgtctactaa tcgatttttc agacgataat tttcctctta ccgtacgttt tcgtacggtt
     1501 ggacctcaaa ccccttaaat aactcaaagg atttttggta atcctgttta acagatttgc
     1561 cttcaaaata aagcattccc aaattgaact gtgcctctgc atatccctga gcagcagcct
     1621 tctgatacaa atcaatctct ttcaggctgt cttttttaat gcctattcct tttccataca
     1681 ggggtccgct tggaaaacgg aaattttcct acgttaagcg ttttgagctt aacgagtgat
     1741 ttcgttccat tgagcgcgaa cccctgaccg taagggatgt acggaacgat ctgactgtcc
     1801 gttaacagtt ttgcttactt tcgtacatgt tattggcatg gtgtacgcaa tcgttcggtt
     1861 tcggacacag aaacgctgta gccccgcgaa ccgagtgata tccaatagcc gattggttaa
     1921 aaagtagtct tagcgtaggt tattttccct tttccaagcg gaccccttca ggggaagaag
     1981 ggcaaggcca aaggataaaa cgcatggcgt ttttccatag gcttcgcccc cctgacgagt
     2041 ttcacaaaaa ccggatggtt ttcggtactc aaatcagtgg tggcgaaacc cgacaggact
     2101 ataaagatac caggcgtttc cccctgatgg ctccctcttg cgctctcctg ttcctgtcct
     2161 gcggcatccg tgttgtggtg gaagctttac ccaaatcacc acgtcccgtt ccgtttagac
     2221 agttcgctcc aagctgggct gtatgcaaga accccccgtt cagcccgact accacgttgt
     2281 tccggtaact atcatcttga gtccaacccg gaacagacac gaaaaaacgc cactggcggt
     2341 agccactggt aactgtttct aggggagcgg agcggtagag gtgagctttc tacaaaaaaa
     2401 catcacctga aaaggctgct taaacttgaa gtaggagcca gcttctggct aaactatctg
     2461 tacaagtttg ggtatctgcg ctcccctttg agccagttac ctcggaacat aagagtttgc
     2521 cgctctgatg ttccggcaaa taaaccgcct tctggcggtt tttctgcgtc tggggctgaa
     2581 gttacgcctc ggcacattat gccgtcaaat gtttttttcc ataaaaacag taaggtgaga
     2641 atagacaaat tacctaactc acctttcaga gaaccacgta acccatcgca aatcctttgc
     2701 ggtgggttac gtggttgaac aagcgcagcg cgttaggcca gcaattgaca agccgggaat
     2761 ccatctctac tatcaatctg ttttgttgac ataaaacacg ctctaagttg attggttgta
     2821 gtggtgccgg ttgctagtaa caaccaggca ccgccttaac atcccttcgt gccctcagat
     2881 gtcttccgca tatatctcac aaggattttt ccattgaata aaggacgttt cattctcgga
     2941 ttttcaatct acaccgccct tcaggcgttc tcggtggggg cgatggctgg caatcaattg
     3001 tgggtcacca ctgatcgcac tgacagacac acttgcccct caaccgaatg cggggtggct
     3061 gggcgactga tgttcagaga gggcgtcgaa gccttggaga agaagggaaa atgggtgcgc
     3121 attactaaga catactcggc gtcctgtgtt ggaggcaaaa gcgagtatgt gagagatggt
     3181 aataaatcct gcacaagtgc taacggaatc aacgatggca agttctccga gtgggtccaa
     3241 tcaaaagatt tgagtgaaaa acgtcctgaa gatccagcag agggcgcgag tggcgatgat
     3301 gtacttgtta aagggtctga cgattacggg atttacaaga cacaattcgc caaggcagcc
     3361 agacaactaa taactgatgg gatctgcact gagggtgact ttcaagaaat gggtggatgg
     3421 atggcttcga cgagtcaggg gtcaggtatg tactttacat attgcggagg gatgacactc
     3481 agcaacaaag tctacctcga cgccaaatca ggtcagacca gccgctgagt gaagacggta
     3541 accactatag atttaccata acgtcccact atacgaatca gcaaaaaaaa tcatgtagat
     3601 aactgcacag agctgtacag gcccacaaaa aagtacaact taactaagtc tgttttgctc
     3661 tttttcccaa tatcgaacac gttcctgtat cctctcatac ttaaatttca gttcgttagg
     3721 aatggcatta gtaccaacct tttcaaacca tttcattcga gcatcgccat ccatctcatc
     3781 ccaaagtaaa ccatgaagtt cactctcagc ctccttgact ttacgtgcag caaaattact
     3841 ccctgacctt ctttctgcat ctgaaacagc aaaaacaaaa tagagtgaaa caaaaaaaat
     3901 cgtaaccacc aatgtggtca gtacattaag gtgaaaatag taaatagcag tggcatcaac
     3961 agcagcaaca atagccgtta caacggcagc aataccaacc aaactaattg attttcctat
     4021 gttcatttta aatccttgtc attatatatt tgaacgccga gtatccattt atatttgcct
     4081 tgatacacga tccaaataca gctcaatatt tatcacattt accataacgc ctcattgggc
     4141 gcaccgctca gacgcgctca gagcgtttct aagcgctaac cctcaaaacc ctacgtctta
     4201 ccctctcaaa acgcctcaga atcgctcctg tgagttttac gggcatgaat cgttatgcac
     4261 cggactgact gacgggcgat ttcgtcccaa aaagccaatc taacccctaa aaccagattt
     4321 ttgcgtgaaa ggagctgcac gcctgcggcg gtcactcctt ccaggctgaa acggtgaacg
     4381 ggaacggatt ttaatccggt ttcgtgaggc gttgaccaac gagcaacgcg aggcggtagg
     4441 gttatttaag ttccttcgcg catgatttta tcttcatgca cttgctgtaa gcggcatctg
     4501 atttttgcac ccagtattca tatacttctt tttgctccat tgcttttgtt gcgtaatacc
     4561 atgcaaaacc accaataaaa caaatactta taatcaaaaa aacagccgtg aaaatagtga
     4621 cggttttccc aacttctttg gtcagcgttt tgttgatgac gttataaata tctttggatg
     4681 actcttttgc ttgattaaga tctttggcga tatctttcgc aacttttagc gactgggagc
     4741 aactggccat catttcattg ttaaaggctt tttgagatgc accaatgtcc tgaatgacgc
     4801 tactcgatac ggccttgcta acttctgacg atacggaatc aacggttttt gatagctcgt
     4861 ttttctgcgt tctgagttct tccgtaactt ttttaaattc tgccagtgag gtgcttaaaa
     4921 ccttctgctg ttcttcaacg acagccatca ttccgtacaa tctttcttca gcgttcataa
     4981 ccatcattcc ctctatctaa gccacggcct ccacgagaaa tatttctttg gctgcttttt
     5041 ttaacctctt tctcgcgggc aatttcctgt tcttttttga atgcctcaaa gccgctcata
     5101 aacgctttct taccttcaaa agcatttttg aacgcctcaa ctccggcacg catttcctgt
     5161 tcgatatttg tcgatttatc tttttcagct atcgcagatc taacatccgt cgataaatca
     5221 ataatgctgt ctcttgcatt gttttctcgt agtaactccc gctctaaaac cctgcgttct
     5281 aggattgcgc tggcctgttc gacagtcatc
//
