LOCUS       NZ_CP115735             5388 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed8, complete
            sequence.
ACCESSION   NZ_CP115735
VERSION     NZ_CP115735.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                :: C_braakii_GW-Imi-1b1
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/09/2024 02:19:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115735.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed8"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            complement(join(5046..5388,1..668))
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26205"
     CDS             complement(join(5046..5388,1..668))
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_097455288.1"
                     /translation="MASYHLTVKSGAKGKGASHAAYISREDKYAKKEKYEDLENKQHGN
                     MPSWAKDNPNLLWQAADNFERANGTSYREFEVALPRELTPDQRLELVNDFVKQEIGDRH
                     AYQFAIHCPNAGIDGKEQPHAHIMFCERINDGIDRDPEHFFKRANSKNPERGGAKKASE
                     PQTHTQRKEALVALRERWAEIQNKHLEKHGCADRVDHRSLKEQGIEREPEKHLGPKFVS
                     SMTVEQASAILERRVLERELLRENNARDSIIDLSTDVRSAIAEKDKSTNIEQEMRAGVE
                     AFKNAFEGKKAFMSGFEAFKKEQEIAREKEVKKSSQRNISRGGRGLDRGNDGYER"
                     /name="MobA/MobL family protein"
     gene            837..1283
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26210"
     CDS             837..1283
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_097455287.1"
                     /translation="MANIKSDEWLQQRLSYIKALKSPSEQQSLIAMLAEKDTLTPPEEK
                     KLNVLIRAEKAQQRAIEARSKAMALINAEKKAAARAERKARDHELFKVAGLLSLVGLVD
                     KETGKPVVDVASLVGALAGLSELPRDNPKWQEWKQKGNEILKGQ"
                     /name="conjugal transfer protein TraD"
     gene            1713..1844
                     /locus_tag="PGH06_RS00030"
     CDS             1713..1844
                     /locus_tag="PGH06_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_097739839.1"
                     /translation="MHEPPVQSDCCAFSGNYRLESNPERHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(2064..2873)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26215"
     CDS             complement(2064..2873)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="WP_151216522.1"
                     /translation="MSKDKKDRSKNRYHQLVEKIFFDKYVDGDLEVLFERLEINSAAKD
                     LGIDLPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPAKELDSSDISNYRMSV"
                     /name="endonuclease"
     gene            complement(2866..4116)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26220"
     CDS             complement(2866..4116)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26220"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222406.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_151216523.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFIFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGITLPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVDAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4520..5065)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26225"
     CDS             complement(4520..5065)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487382.1"
                     /translation="MMVMNAEERLYGMMAVVEEQQKVLSTSLAEFKKVTEELRTQKNEL
                     SKTVDSVSSEVSKAVSSSVIQDIGASQKAFNNEMMASCSQSLKVAKDIAKDLNQAKESS
                     KDIYNVINKTLTKEVGKTVTIFTAVFLIISICFIGGFAWYYATKAMEQKEVYEYWVQKS
                     DAAYSKCMKIKSCAKELK"
                     /name="hypothetical protein"
     Typing          1452..1841
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.388"
                     /coverage="90.0"
                     /evalue="1.05e-168"
ORIGIN
        1 gagctaacaa atttagggcc taaatgtttt tccggctcgc gctcgatgcc ttgctctttc
       61 agtgaccgat gatcaacacg gtcggcacat ccgtgttttt caagatgctt gttttggatt
      121 tccgcccaac gttcacgcaa cgcgacaagc gcctctttcc tctgcgtgtg cgtttgtggt
      181 tcgctcgctt ttttcgcgcc gcctcgttct ggattcttac tgtttgcacg tttgaaaaag
      241 tgttcaggat cgcgatctat accgtcgtta attcgctcgc aaaacatgat gtgcgcgtgt
      301 ggctgttctt taccatcaat ccccgcgtta gggcagtgaa tagcgaattg gtaagcatgg
      361 cgatcgccta tctcttgttt tacgaaatcg ttcaccaatt caaggcgctg atcgggtgtc
      421 aattctctgg gtaatgcaac ctcaaattca cggtaacttg ttccgttagc gcgttcgaaa
      481 ttatcagccg cttgccatag caaattcgga ttatccttcg cccatgaagg catattcccg
      541 tgttgcttgt tttctaggtc ttcgtacttt tcttttttgg catatttatc ttcgcgtgaa
      601 atataggcgg catgggaagc acccttcccc tttgcgccgc ttttcactgt cagatgataa
      661 gatgccatga tgttgacctt tagccctcgg caggagcgca cagtttctct aacgaagtga
      721 gaaactacta agtgcgcctc tctaaaaaat tagagaggat tgtagtgcct tttcgccgtt
      781 ggttccagat gataaagtaa tcaaaatcgc aataaaccaa acaaggaaaa atcaacatgg
      841 ctaacatcaa aagcgatgaa tggttacagc aacgattgag ctatatcaag gcgctgaaat
      901 caccaagcga acaacagagc ttaattgcga tgctggcgga aaaggacacc ttaacgccac
      961 cggaagaaaa aaagcttaat gtgctgattc gagcagagaa agcacaacag cgagcaattg
     1021 aggcgcgttc gaaagcaatg gcgctgatta atgcagagaa gaaagcagcc gcaagagcag
     1081 agcgtaaggc gagggatcat gaattattca aggtcgccgg attgttaagc cttgttgggt
     1141 tggtggataa agaaacgggc aagccagtcg tggatgtggc ttcgctagtc ggtgcgctgg
     1201 ctggcctgag tgaattacca cgcgacaacc cgaaatggca ggaatggaaa cagaaaggca
     1261 atgagatctt gaaagggcaa tagcgcgtaa gtgcttccct gtcagaactg agaaattacg
     1321 cttcctcgct cattcgctca ctgcgttcgg tcatgagact gcggcgagcg ttgcgggcac
     1381 actcaaaacc ggaagtttag ttatcaacag attgagtgga taacagggct ggcagggtgt
     1441 ggaagaaaag acaagaaagg ccacggagtt gccgtttttc cataggctcc gcccccctga
     1501 cgagtatcat aaaatctgac gctcaaatca gtggcggcga aacccgacag gactataaag
     1561 atcccaggcg tttccccctg gtagctccct cgtgcgctct cctgttcctg cctttcggtt
     1621 taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt
     1681 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact
     1741 gctgcgcctt ttccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcgc
     1801 cactggcagc agccactggt aatcggattc acggagcgga gtagagttat ctacagagtt
     1861 cttgaagtgg tggcctgact acggctacac tgaaaggaca gttttggtat ctgcgctccg
     1921 tgaaagccag ttaccttcgg aaggaagagt tggtagctct aaattccggc aaacgaacca
     1981 ccgttggtag cggtggtttt tttgtttacg gcttaggaga ttacggctag aacgaaagga
     2041 tctcaagaag atcctctcag aaatcataca gacatgcggt agtttgagat gtctgagcta
     2101 tctaactctt ttgccggaac cagtttgtaa tgcctttcct caatcaattt cacttgtcct
     2161 tcgtctatct ttagctcaaa cagtgcaatc aaatcgctcg aaacaaactg agcggaaata
     2221 gctctacatc taacatttgg gtatttttgt tcacaccacg ctgtatcttg cgatgtttgc
     2281 accattgata tttgatcggt tccacctttt gcttgaactg gtattatata atggcaaccg
     2341 tacttatcta tacctacata aagctcatcg atctctattt gaccaatccc cttcaccgta
     2401 gttcttaaat gattctgtaa agaatatgtg gttataccta gaaatatatc tattagtcgg
     2461 ttatatctca ccttcgccag caaagcctgc tcgtcgtcta gagaataacc tgtaattatc
     2521 tccggggtat tatctggtat ttttattgcc accatattag gatttggcaa aatcctacta
     2581 atagttacca atctaaatcg atactttgac ctgccagcca gctcaataat ccactcctta
     2641 ccttctggct gtgtcaacag aatactttct ggcataggcg ttctaaatct gattgaatat
     2701 ataacatccc caacattaga tgggagatct atacctaaat cttttgcagc actgtttatc
     2761 tctaaccttt caaacaaaac ctctaagtca ccatcaacat atttatcgaa aaatatcttt
     2821 tctaccaact gatggtatct attttttgac ctatcttttt tatccttact cacaaacaac
     2881 ctccttgtta ctatttttta ttctcttagc aattacattt gatgacacac caaacataga
     2941 tgcagcatcg gacatagaaa tagcaactaa actttcatca ccgagtttga ttttcttcgt
     3001 tggttttttt ggcgtaacac caagaacctt cataatctgc aagccaacac ttctagctag
     3061 cataggagga acactattac cgacctgtct gaatccatgc cacttagtca catgaaatct
     3121 gaaccaatca ggatatgaat gcagtcttgc tgcctctctg accgtgatga ccctaggata
     3181 gatcggatgt ataggacgag gagatgtaaa agcacctctg tcacttgctg ttcctgctct
     3241 taaagtatta cataacccat tcatcgagag ctttttgaac ctgctgattg gttctaccgt
     3301 gtcaggcaaa gtttcaacaa accgccctct agaaatgtca gagtgatctg tcagtaaaga
     3361 acacgttaat ttgtctttat taaaaatcct tgggtagcta aaatcactag ggtcttttcc
     3421 tacgccatgc aaataggatg agtatagtga ttgctcacca aatttagcat caacccaatc
     3481 tctaccccat agctcaggat atttttccgc atcagggata tccccaatag cgtccccaac
     3541 cgttgttcta atggataatg gctcagggta tgatggtaga gtaattcctt tccttccccc
     3601 cattaagaaa agacgacggc gatcttgagg aactccataa tcagctgcat ttagcacttt
     3661 ataatcactt acaacgtcgt acccgccatc tttaaatgca tccattatct ctactaaaaa
     3721 ctgcttatgc ttaccaaccg tcagcccttt tacattctca aaaataaagt atttaggctt
     3781 tagctcaaga accagccgta catagtgata gacaagtttg tttctatcat catcaagcgc
     3841 gcgctttcct attaatgaaa agccttggca tggagcgccg ccgatcacta catcaatctc
     3901 tttctcacca atgcctgaaa cttccctaac gtattccccg gtaacatcga caacacttct
     3961 acatatcgtt ttgcacatag gaaagttata tttgtgagca gagcaatgta ttgggtctat
     4021 ttcaactgat gccattatat caaagccagc ctgctcgaaa ccgagactca gtccacccgc
     4081 tccagcaaat aaatcaacag caatcggtct agtcattctt tacacccacg ttatataaaa
     4141 tcaacgtgat gctatcagaa aatcgatcgc tttaccgatc gcttttctga tatcggttga
     4201 acataacatc tcttgggcgc accgctcaga cgcgctcaga gcgtttctaa gcgctaaccc
     4261 tcaaaaccct acgtcttacc ctctcaaaac gcctcagaat cgctcctgtg agttttacgg
     4321 gcatgaatcg ttatgcaccg gactgactga cgggcgattt cgtcccaaaa agccaatcta
     4381 acccctaaaa ccagattttt gcgtgaaagg agctgcacgc ctgcggcggt cactccttcc
     4441 aggctgaaac ggtgaacggg aacggatttt aatccggttt cgtgaggcgt tgaccaacga
     4501 gcaacgcgag gcggtagggt tatttaagtt ccttcgcgca tgattttatc ttcatgcact
     4561 tgctgtaagc ggcatctgat ttttgcaccc agtattcata tacttctttt tgctccattg
     4621 cttttgttgc gtaataccat gcaaaaccac caataaaaca aatacttata atcaaaaaaa
     4681 cagccgtgaa aatagtgacg gttttcccaa cttctttggt cagcgttttg ttgatgacgt
     4741 tataaatatc tttggatgac tcttttgctt gattaagatc tttggcgata tctttcgcaa
     4801 cttttagcga ctgggagcaa ctggccatca tttcattgtt aaaggctttt tgagatgcac
     4861 caatgtcctg aatgacgcta ctcgatacgg ccttgctaac ttctgacgat acggaatcaa
     4921 cggtttttga tagctcgttt ttctgcgttc tgagttcttc cgtaactttt ttaaattctg
     4981 ccagtgaggt gcttaaaacc ttctgctgtt cttcaacgac agccatcatt ccgtacaatc
     5041 tttcttcagc gttcataacc atcattccct ctatctaagc cacggcctcc acgagaaata
     5101 tttctttggc tgcttttttt aacctctttc tcgcgggcaa tttcctgttc ttttttgaat
     5161 gcctcaaagc cgctcataaa cgctttctta ccttcaaaag catttttgaa cgcctcaact
     5221 ccggcacgca tttcctgttc gatatttgtc gatttatctt tttcagctat cgcagatcta
     5281 acatccgtcg ataaatcaat aatgctgtct cttgcattgt tttctcgtag taactcccgc
     5341 tctaaaaccc tgcgttctag gattgcgctg gcctgttcga cagtcatc
//
