LOCUS       NZ_CP115734            10155 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP115734
VERSION     NZ_CP115734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                     :: C_braakii_GW-Imi-1b1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:34:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 17
            CDSs (total)                      :: 17
            Genes (coding)                    :: 16
            CDSs (with protein)               :: 16
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115734.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10155
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed7"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            join(9965..10155,1)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26125"
     CDS             join(9965..10155,1)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1..>153
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26130"
                     /pseudo=""
     CDS             1..>153
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(397..768)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26135"
     CDS             complement(397..768)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487391.1"
                     /translation="MRINIAKGNHRVKRLIIAAVMAVTLAPLAQAKNAQAVDTRVNGVC
                     GAMVDALQMADPNVVFDMCWRGARDAYQGSPTTCPKKIKQFEQQAEKLDGMKRGEYVQI
                     GEAYRAGCNIGKSSKDAGY"
                     /name="hypothetical protein"
     gene            complement(779..1495)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26140"
     CDS             complement(779..1495)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050875900.1"
                     /translation="MKRLIIAAVMTAALTPAAQAANVDCARPLTSFEYKIVPMGSTYYA
                     AYPQQFNSVMTEFCTRGQQARAAGESGQQVAARFDIEGRQWVARTFGRDTMKQKEFDGL
                     VDLLSSAGLRGYMDQMPAEPNAPRKSNAVMNATPAQQAFYKTNSIDTADPQVIKYANAA
                     LARVTGLTKAARAKHTGEMCYLIVTSNKEGMITGTRDDGGDAGFCAALLEAVKGAKMPP
                     IPGSSDHLQSLTYVFE"
                     /name="hypothetical protein"
     gene            complement(2619..2780)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26145"
     CDS             complement(2619..2780)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487392.1"
                     /translation="MTTAEIEKIHTEITRLMAQTSEISRETMWYPVLVATGLIGAVATV
                     TTILINLF"
                     /name="hypothetical protein"
     gene            complement(2794..3252)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26150"
     CDS             complement(2794..3252)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_272487395.1"
                     /translation="MANVKNEEWLNQRISYIKSLKSPNEQQRLIVLWSEKENLEPAEEK
                     KLALLIRAERIQQRAAESRVKVNAVLNADKKVAAKAERKARDHELYKSAGLMVLAGLVD
                     KETGKPVTDVAALVGALAGLNELPRDNPKWQEWKRRGNELLNSGSPKT"
                     /name="conjugal transfer protein TraD"
     gene            3418..4461
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_26155"
     CDS             3418..4461
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_272487393.1"
                     /translation="MASYHLTVKTGEKGCGASHASYISREDKYANKDKYEDLEHTQHGN
                     MPSWAKDNPLLLWKAADEFERANGTTYREFEIALPRELTKEQRQDLVNEFIKNEIGEKH
                     AYQYAIHCPKAGIEGKDQPHAHIMFCERINDGIERTPDQFFKRANTKNPALGGAKKANV
                     SQSYTQRKEALTDLRSRWADVQNQHLEKHGHAVRVDHRSLKAQGIDREPERHFGPKRVR
                     DLTVEQASTILESRVLERELERVSIVRDRLIDLSGDLHAALAERQEKSLQPQPVAESTP
                     APDIQDQLRAGMEAFRREFETRKEFMKGVETYKQEQERQREKERQERLRKELSRGHGND
                     RDQGYER"
                     /name="MobA/MobL family protein"
     gene            4458..4994
                     /locus_tag="PGH06_RS00040"
                     /old_locus_tag="PGH06_26160"
     CDS             4458..4994
                     /locus_tag="PGH06_RS00040"
                     /old_locus_tag="PGH06_26160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265497824.1"
                     /translation="MMDETEKKLYQMAIVIEEQQKTLDASLKKIDVAVAALAQERAELA
                     KTVENISKEVGKSAAQSLINALGEAQVRFNHEMNKTANLALKSAQDTNKELEIAKKNVA
                     TFTRGLEGTLSAEMNRAATKYFTMFAVLGVLLFGGLYAYGAYLAYEELQSAAFWKDQSD
                     AAYEKCVKTKGCIKK"
                     /name="hypothetical protein"
     gene            4945..5535
                     /locus_tag="PGH06_RS00045"
                     /old_locus_tag="PGH06_26165"
     CDS             4945..5535
                     /locus_tag="PGH06_RS00045"
                     /old_locus_tag="PGH06_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487394.1"
                     /translation="MQPTRSALKQRDALKNEVDMVMFDDELEHRTVAELIRSLYAIVDE
                     FERRNRGRRFTPDGHLVGSIGEIIAAEWYGLTLYPASAETHDAKSPCGREVQIKVTQNE
                     TGQVGLRSEPEHLLVLYLARNGSVSELFNGPGALVWNAAGRMQKNGQRPISARKLRILV
                     AGVSTTERINPVERAYPLKVTDEHFVESATATE"
                     /name="hypothetical protein"
     gene            complement(5636..6067)
                     /locus_tag="PGH06_RS00050"
                     /old_locus_tag="PGH06_26170"
     CDS             complement(5636..6067)
                     /locus_tag="PGH06_RS00050"
                     /old_locus_tag="PGH06_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_265497821.1"
                     /translation="MAKTLLNLSQASQAAGITRRTLYNHIKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNLPEKQIPTISHRENTQKNFPLEQFQSMQKELMELKQAVTLMLEDKSTR
                     EEERRKNDEERRKLQEEVNNLSKALEQTRKKGFWSRLFS"
                     /name="hypothetical protein"
     gene            6630..6875
                     /locus_tag="PGH06_RS00055"
                     /old_locus_tag="PGH06_26175"
     CDS             6630..6875
                     /locus_tag="PGH06_RS00055"
                     /old_locus_tag="PGH06_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_047359280.1"
                     /translation="MHAITFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAAHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            6856..7131
                     /locus_tag="PGH06_RS00060"
                     /old_locus_tag="PGH06_26180"
     CDS             6856..7131
                     /locus_tag="PGH06_RS00060"
                     /old_locus_tag="PGH06_26180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_072058806.1"
                     /translation="MSKWNIKFTKNGADDINYWRGTDKKKFERIRQLLHSIESDPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
     gene            7209..8237
                     /locus_tag="PGH06_RS00065"
                     /old_locus_tag="PGH06_26185"
     CDS             7209..8237
                     /locus_tag="PGH06_RS00065"
                     /old_locus_tag="PGH06_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663643.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_272487386.1"
                     /translation="MRTHYDNLKVSQDAPVEVIQAAYRTLAKKYHPDLNRDNPDAEKIM
                     QILNEAYEVLSDPVRRKEHDSWIARKNWERRIESSVASKKSDSSEKGKESVSAGKKIRT
                     VGLTCAIFVKKIISVVVFYFLGLSKRFLLIGLAGGVVYFFSSEDGYFRSEVGRGVSAFF
                     KKITPQEVDIPSGDVNVKPWEYITSNNEKTSVNYISDGACGGPVSNWVYKGVASPWPEY
                     PQVLRDLDKRKGLSSLTLDNTRNNQRLWVKLSLAKDSSTTNFVREAFIPAGRSLVFDKI
                     NAGDYVVKVKDIDSGCAQISQVIGFVEREIVGGTEYSDSVLTFYPVVNGNTHFNTLPNS
                     QF"
                     /name="J domain-containing protein"
     gene            complement(8382..8684)
                     /locus_tag="PGH06_RS00070"
                     /old_locus_tag="PGH06_26190"
     CDS             complement(8382..8684)
                     /locus_tag="PGH06_RS00070"
                     /old_locus_tag="PGH06_26190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487387.1"
                     /translation="MKVGNVREYGPTKSHLLLMITTFPAGVGVFIFPVVEAVWGEMSLL
                     KVFGYTSVSFIAGGFVGNMIVRVLISRSIGFLFINPLAMLFSFSGIMLLIMKYDQ"
                     /name="hypothetical protein"
     gene            complement(8744..9094)
                     /locus_tag="PGH06_RS00075"
                     /old_locus_tag="PGH06_26195"
     CDS             complement(8744..9094)
                     /locus_tag="PGH06_RS00075"
                     /old_locus_tag="PGH06_26195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487388.1"
                     /translation="MKSMIYRLVILSISLLVVGCVSTSNVLELGRDTYTVSATADGFRD
                     AASARQSAFKAGAEKCNSLSKKFLLTNENVANTRMGIDTTVTLYFRCLDERDSEYVRPH
                     LQKAPDLLIESK"
                     /name="hypothetical protein"
     gene            complement(9075..9224)
                     /locus_tag="PGH06_RS00080"
     CDS             complement(9075..9224)
                     /locus_tag="PGH06_RS00080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487389.1"
                     /translation="MPPPEVKPAPLRDTSYQNKIIEPIDFNKKKNRYNHDPQSPNLGNE
                     INDL"
                     /name="hypothetical protein"
     gene            complement(9436..9549)
                     /locus_tag="PGH06_RS00085"
                     /old_locus_tag="PGH06_26200"
     CDS             complement(9436..9549)
                     /locus_tag="PGH06_RS00085"
                     /old_locus_tag="PGH06_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073511950.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHANAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(9437..9844)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.844"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atggccagag tgtctattag tgaggctgcc cgactgacgg gtaagagtag aacgacactg
       61 cacaggttaa ttaaagcagg ttacctgtcc acctgtagcg gtgaacgcaa tgcaaaaatg
      121 gtagatactt cagagctttt acgtgtcttt ggggttcgag ggctacccgt gcgaaaaacc
      181 acgttaagaa actaacagtt tgaaatatca agacttatga ggctatgtgt ttggggcgga
      241 aaagatcttc caaatatcgg ttattggaca ttttgagact ggatcgtatc aaaatcgatt
      301 tctgataccg cttagcgtgg tttttcacac cactggactt aaaacccctg ttatagctgc
      361 cgacactacg gcggccacaa ttacgcgttt catgatttaa tagcctgcat ctttcgatga
      421 ttttccaatg ttgcagcccg cgcggtacgc ctcgccgatc tgtacgtatt cgccgcgctt
      481 catgccgtcc aatttttcgg cctgctgctc aaactgtttt attttcttcg ggcatgttgt
      541 cgggctgccc tggtatgcat cgcgagcgcc gcgccaacac atatcaaaaa cgacgtttgg
      601 atctgccatt tgcaaggcgt caaccattgc gccgcatacc ccattaaccc gcgtgtctac
      661 cgcttgcgcg ttcttcgctt gcgctaacgg tgcaagtgtt accgccatca cggcggcgat
      721 gattaacctc ttcactctgt ggtttccttt ggcgatatta attcgcaagc cctacagcct
      781 attcaaagac gtaggtaagg ctttgcaggt ggtcgctgct gcctggtatc ggcggcattt
      841 ttgcgccttt caccgcttcc agtaatgccg cacaaaagcc cgcgtcgccc ccgtcgtcac
      901 gcgttccggt gatcatgcct tctttgttgc tggttacgat caggtagcac atttcgccgg
      961 tgtgttttgc acgagcggct ttcgttaaac ctgttacacg ggcaagcgct gcattcgcgt
     1021 acttaataac ctgcgggtct gcggtgtcga tgctattggt tttataaaaa gcctgctgcg
     1081 ctggcgtcgc gttcatcact gcgttgctct tacgcggtgc gtttggctcg gcgggcattt
     1141 gatccatgta gccgcgcaag cctgcggaag aaagcagatc aacaagcccg tcaaactcct
     1201 tttgcttcat tgtatcgcgt ccaaaagtac gcgctaccca ctgccgcccc tcgatatcaa
     1261 accgcgcggc tacctgctgg ccggattcgc cagccgcgcg cgcttgctgc ccgcgagtac
     1321 aaaactcagt cattacggag ttaaactgct gtgggtaggc ggcgtaatac gtcgaaccca
     1381 tcggcacaat cttgtattca aaactggtaa gcgggcgcgc acaatccacg tttgccgcct
     1441 gcgctgccgg tgtaagtgct gccgtcatca cggcggcgat aattaaacgt ttcatcggat
     1501 attctcaatg agttcaacaa gattgattgt ctgcgctgag tttctgggct cagcttggga
     1561 aacgggattt tttgtacgtt aagagcatta ctctcccaaa aatagacaca attgactgat
     1621 ttaacgtggt cagttatcgc ttttaagtca tctttctcgg tgattcgcat ttaggttatg
     1681 gcaaacagca tgacagttac atagaattat ttatacggtc tttaacgggt gtttttctaa
     1741 attttatcag aatgttacgc tcaaatgagc gcaactatct gtgatagagc acatcattta
     1801 aatgatcttc ttgagatcct ttttatctgc gcgtaatctc tcgtcctgta aacgaaaaaa
     1861 ccacctgggg aggtggtttt tcgaaggttc agtaagttgg ggaacttctg aaccatggta
     1921 actggctctg gcggagcgca gtaacctaat ctgtccttcc agtgtagctg tagtttggct
     1981 cctacttcaa gaactccgta tatattcgct ccgccaacct tttaccagtg gctaccgcca
     2041 gtggcgattc gtcgtgtctt tccgggttgg atgcaagtga acagttaccg gaaaggcgtg
     2101 gtagtcggac taaacgggga attcgtgcat atagtccagc ttggagcgaa ccgtctaaat
     2161 ggaatcgagt gtctggcgtg gaatgagaga acgcggcctg taacagcgga atgacaccga
     2221 tacaccacaa gacgggaaca ggagagcgca cgagggagcg acccaccgga aacggagggg
     2281 atctttaagt cctgtcgggt ttcgcccgta ctgtcggatt catggttgag cgtaagctcc
     2341 cacagatgca cctgaaaagt gtctgtttat gtgaaatctg gcaggagggc ggagcctatg
     2401 aaaaaacggc aacgccggag ccttttcccg ggtttgcctt ttcggttcgt accacactga
     2461 ccgtttacgt tatctgttca ttagaatccc accgctcgcc gcagactcat gaccgaacgt
     2521 agtgagcgaa tgagcgagga agcggaagag tggctgatct catctctggc acggtcactc
     2581 ttccgcttct ctgttcactg ctactttttg gacttctttt aaaacaagtt gatgaggatg
     2641 gtagtgactg tagccactgc acctattaag ccagttgcga ccagaaccgg ataccacatg
     2701 gtttcccggc taatttctga tgtctgtgcc attaatctgg tgatttctgt atgaatcttt
     2761 tcgatttctg ctgtggtcat ctcatgtcat cctttaggtt tttgggctgc cactgttcag
     2821 taactcattc ccccggcgtt tccattcctg ccatttcggg ttatccctcg gtaattcatt
     2881 gagtcccgcc agcgcaccga ccagggcagc aacgtcagtg acgggttttc cggtttcctt
     2941 gtctaccagt cccgcgagta ccatcagccc cgctgattta tagagttcat gatcccttgc
     3001 tttccgttcg gccttcgctg cgaccttttt atccgcattc agtaccgcat ttactttcac
     3061 tcgtgactca gctgcccgct gctggatcct ctcggctctg atcagtaagg ccagtttttt
     3121 ttcttctgcg ggttccaggt tttccttttc agaccacaac actataagac gttgttgttc
     3181 gttgggtgat ttcagtgatt tgatataaga gattcgctga ttcaaccatt cctcattttt
     3241 tacgttggcc atgccattat tccttgtttt aattgttgtc attttgttta ttttatcatc
     3301 tggaatcaaa ggagaaaagg caatacaatc ctctctaatt tttagagagg cgcacttagt
     3361 agtttctcac tttgtgagag aaactgtgcg ctcctgccga gggctaaggt caacatcatg
     3421 gcatcttatc atctgacagt gaaaacaggc gaaaagggct gcggtgcatc ccatgcgtcc
     3481 tatatttcac gtgaggataa atatgccaac aaagacaagt acgaggactt agaacacacg
     3541 caacacggga atatgccttc gtgggcgaag gataatcctt tattgctctg gaaagcggct
     3601 gatgaatttg aacgtgccaa tggaactacc taccgggaat ttgaaatcgc attacccaga
     3661 gaactgacga aagagcagcg tcaggatctg gtgaacgaat tcattaaaaa tgagataggc
     3721 gaaaaacacg cttaccagta cgccattcac tgcccgaagg ccggaattga agggaaagat
     3781 cagccacacg cccacatcat gttttgtgag cggattaatg acggtattga acgcaccccg
     3841 gatcagtttt ttaaacgtgc caataccaaa aatccagcac tgggcggcgc gaagaaagcc
     3901 aacgtctcac agagctacac gcaacgaaaa gaggcactga cagatctccg ttctcgctgg
     3961 gctgatgtgc agaaccagca cctggagaaa cacggtcatg ctgtccgggt ggatcaccgt
     4021 tcgctgaaag cgcagggcat cgaccgcgaa ccggagcgcc atttcgggcc aaagcgcgta
     4081 cgtgatctga ccgtggagca ggccagcacc attctggaga gtcgcgttct ggaaagagaa
     4141 ctggaacggg taagcattgt ccgggatcgt ctgattgacc tgtccgggga cttacatgca
     4201 gctctggcag aacgacagga aaaatcatta cagcctcaac ctgtggctga gtcgaccccg
     4261 gcacctgaca tacaggatca gttacgcgca gggatggagg cattcagacg cgagtttgaa
     4321 accaggaaag aatttatgaa aggcgttgag acatacaagc aggaacaaga acgtcagcgg
     4381 gaaaaagagc ggcaggaacg actcaggaaa gaactttcac gcggacacgg aaacgaccgg
     4441 gatcagggct atgaacgatg atggatgaaa cagaaaaaaa gctataccag atggcaatag
     4501 tcattgaaga acaacaaaaa actctcgatg catcactgaa aaaaatcgat gtagcagtcg
     4561 ccgcactggc acaagaacgg gcagaactgg ccaaaactgt ggaaaacatc tcaaaagaag
     4621 ttgggaaatc cgcggcgcaa tcgctgataa atgctctggg agaggctcag gtgcgtttta
     4681 atcacgaaat gaataagact gcaaaccttg cgttaaaatc ggctcaggat acgaataagg
     4741 agcttgaaat agccaaaaaa aacgtagcta cgttcacccg aggtctggaa ggaacgcttt
     4801 cagccgagat gaacagggcg gctacaaaat atttcaccat gtttgcagtg ctgggggtgc
     4861 tactttttgg cggcctgtac gcgtacggcg cgtaccttgc ttacgaagaa cttcaaagcg
     4921 cagcattctg gaaagatcag tcagatgcag cctacgagaa gtgcgttaaa acaaagggat
     4981 gcattaaaaa atgaggttga tatggtgatg tttgatgatg aactggagca cagaactgtc
     5041 gctgaactga ttcgatcgct gtatgcaatc gtcgacgagt ttgagcgacg gaatcgtggc
     5101 cgcaggttta cacccgacgg ccatctggtg gggagtattg gggaaatcat tgccgctgag
     5161 tggtatggac tgacactgta cccggccagt gctgagacac atgatgccaa gtctccttgc
     5221 gggagagaag tgcagatcaa ggtaactcaa aatgaaactg gccaggttgg tttacgctca
     5281 gagcccgaac atctccttgt cctgtacctg gcccggaatg gaagtgtcag tgaacttttc
     5341 aacggtccag gtgcattggt ctggaatgca gcaggcagaa tgcagaagaa tgggcaacgg
     5401 cctatttcgg cacgaaaact gagaatccta gttgcaggtg tttccacgac cgagagaatt
     5461 aatccagtgg aacgagccta cccgttgaag gttacagatg agcactttgt tgagtctgct
     5521 acagcgacgg aataatgtca gaaagaataa tgccccatgt tgtaatttgt agtcattgtg
     5581 atcaggggga tttagattgt gagccgctac acgtatctat aaccaggaag gctggtcagc
     5641 taaacagccg cgaccagaaa ccttttttgc gcgtttgttc cagtgcctta ctgagattgt
     5701 ttacctcttc ctgtagcttc ctgcgttcct cgtcattctt gcggcgttct tcctctcgag
     5761 tgcttttatc ttcaagcatg agggtgacgg cctgtttcag ctccatcagt tccttttgca
     5821 tcgactgaaa ctgctcaagt gggaaatttt tctgtgtatt ttcacggtgt gaaatcgtgg
     5881 gaatttgttt ctcaggtaag tttacatttc cgtagacccg tatcagctct gatacatcca
     5941 cgaccgggtt gtttttccca tcgcgtgaca cggtcacttt accttgtttt atatggttgt
     6001 agagggttct gcgggtgatg cctgcggcct gcgatgcttg tgaaaggttg agtaatgttt
     6061 ttgccactgt ataccctcag tttctgttat gtgtgtaagg tgtaaaattt cccacgagaa
     6121 gtataaccct gtttttacct taatacggta cccctaacgt cctgcggttg ttcaaaccac
     6181 caacgcctca cgtctgcaca gtaaactgcg cacccgttca ccgttatcat ttattcagtt
     6241 ttgtggtgga taagtccgtg ggaaagctca gtaaaggtgg ctggcagcgg cggtatccgg
     6301 gcttgacggt atggtgagtg aaaagtgacc agcaacgaac agccggtatc tgcacgggga
     6361 aaagtcggta aaaccccaat caggccatcc tttcgccccg aaacaggacg tcatgcagcc
     6421 acgtgcatag ccatgcacct gcttaaaact tatcaggggc gttttgcggg atttccgcca
     6481 gggataaggc agtctttttc cgcaaaacgc gcaggatcgc gctgagggcg ttttcgcggt
     6541 gcgtgtaata agggattatg gtaaattcta tatgtactta attgcgtaca tgttctatac
     6601 tggtactaca actgaaggga gccaataaga tgcatgcaat cacttttacg gatgcccgta
     6661 aacattttgc cgataccatg aagcgtgtta ccgatgatgc tgagccagta cgagtaatgc
     6721 ggcgagatgc acctgatatc gtcatgatcg atgctggaga gtatgaggca ctcatagaaa
     6781 cagtgtatct gttcagcaat cctgcgaatg ctgctcatat caatgagtca atgaaacagg
     6841 cggagcgcgg cgaatttgtc gaagtggaat attaagttca cgaagaatgg cgctgatgat
     6901 atcaattact ggcgtggtac tgacaaaaag aagtttgaac gtatccggca gctacttcac
     6961 agcatcgagt ctgatccgtt aactggtata gggaagcctg agcgattacg ccatcacaaa
     7021 gatcctgcgc tctattctcg gcgtatcgat cagagccata ggcttgtata ttcaatccaa
     7081 aacggagaaa ttatcatcta cgcagcgcgt tatcactacg gcgataaata gaaataaaaa
     7141 tattttacat ttagctatcg ggtttatatt ttttttctaa aaggattttt ccaactggtg
     7201 attttaaaat gagaactcat tacgataatt tgaaggtgtc tcaggatgca cctgtcgagg
     7261 tgattcaggc tgcttataga acccttgcaa aaaaatacca tcctgatttg aatcgagata
     7321 atccggatgc ggaaaaaatc atgcagatat taaacgaagc ttatgaggtg cttagtgatc
     7381 cagttaggag aaaagaacat gattcatgga tagcgaggaa aaattgggag cgcagaattg
     7441 aatctagtgt tgcaagtaaa aaatctgact ctagtgaaaa aggaaaagaa tctgtcagtg
     7501 caggaaaaaa aataagaaca gtgggtttaa cctgtgctat ttttgttaag aaaattatct
     7561 cagtggtcgt tttttatttt ttgggtttat caaagaggtt tttgttaata ggtctggcag
     7621 ggggtgttgt ttattttttt tcatctgaag atggttattt taggtctgag gtaggaaggg
     7681 gggttagtgc ttttttcaaa aaaataacac cacaggaagt tgatattcca agtggtgatg
     7741 tgaatgtaaa accatgggaa tatataacct ctaataatga aaagacaagt gttaattata
     7801 taagtgatgg tgcgtgtggt ggtcctgtta gtaactgggt ttacaaaggt gttgcttcac
     7861 catggcctga ataccctcag gttcttcgcg atctggataa aagaaaagga ttgtcatcac
     7921 tcactttaga taatacgcgt aacaatcaac ggttatgggt taagctctcc ttggcgaagg
     7981 actcttccac gacgaacttt gtgcgtgagg cttttatacc ggctggcaga tctttggtgt
     8041 tcgataaaat taacgctggt gattatgttg tgaaggtaaa agatatcgac agtgggtgtg
     8101 ctcaaatatc tcaggtgatt ggttttgttg agagggaaat agttggtggt actgagtata
     8161 gtgattctgt attgactttc taccctgttg ttaatggtaa tactcatttt aacacattgc
     8221 caaactctca gttttagtta tatttgcatg ataagtgttt tttgtttcgt ataattgaat
     8281 gggattttat ctttactatt ctggtgggaa taaacttgat tctatggtgt agtcctcgct
     8341 ggggtgagat aactatatag ttttattttt ttcaattaat gctattgatc atatttcatg
     8401 atcaatagca ttataccaga aaagctgaag agcattgcca gtgggtttat gaacaagaac
     8461 cctattgatc ttgaaattaa tacacgaaca atcatattac caacaaaccc tccggcaatg
     8521 aatgatacgg aggtataacc aaaaactttc agtaatgaca tttctcccca tacagcttct
     8581 actaccggga agataaaaac gccaacgcca gccggaaatg ttgtaatcat caacagtaaa
     8641 tggcttttag tggggccgta ctctctgaca tttcctactt tcatcaagtg tgtgatgaat
     8701 gaagctgtcc accataggat cataaccata aggaagttca ttgttattta ctctcaatga
     8761 gtagatctgg tgctttctgt agatgaggcc gtacatactc gctgtctctt tcatcaagac
     8821 aacggaaata gagtgtcact gtggtatcta ttcccattct tgtgttagcc acgttttcgt
     8881 tagtaagcag gaattttttg ctgaggctat tacatttctc cgctcctgct ttaaaggcac
     8941 tttggcgtgc tgatgccgca tctctaaaac catctgcagt agctgaaact gtgtatgtat
     9001 ccctacctaa ctcaagaaca ttgctggtag aaacacaacc aactacgagt agcgatattg
     9061 ataaaattac taatctataa atcattgatt tcattaccca agttgggtga ttgtggatca
     9121 tgattatatc tgtttttttt cttattaaaa tcaatgggtt caataatttt attttgatag
     9181 ctggtatcgc ggagtggtgc tggtttcact tcagggggag gcatatggaa caggatcctc
     9241 ttgagatcct ttctgtagca tcgtaatctc ttgctctgta aacgaaaaaa ccgcccagca
     9301 aggcggtttt ctcgaaggtt ctcagagcag caactctttg aaccgaggta actggcttgg
     9361 aggagcgcag tcaccaaaac tgttcgttta gtttagcctt aaccggcgca taacttcaag
     9421 actatctcct ctaaatcagt taccagtggc tgctgccagt ggcgcatttg catgtctttc
     9481 cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact gaacgggggg
     9541 ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaactgagtg tcaggcgtgg
     9601 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     9661 gagagcgcac gagggagcca tcagggggaa acgcctgata tctttatagt cctgtcgggt
     9721 ttcgccacca ctgatttgag cgtcagtttc tgtgatgctt gtcagggggg cggagcctat
     9781 ggaaaaacgg ccgctgcgcg gcctttcctt ctttccacca tgcgattgct cggttttctc
     9841 gagcgtttct acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact
     9901 gagtgagcga ggaagcggaa aagagaacat ccctacactg agcactgacg ctctgaggcc
     9961 tcttatgacg aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc
    10021 tctgctcctg ctggagaagc ttaacgagct tgatctcgat gtcgaagccg acctttgtga
    10081 gaaactccac gatgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt
    10141 acaggatggt aactg
//
