LOCUS       NZ_CP115733            11263 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP115733
VERSION     NZ_CP115733.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                     :: C_braakii_GW-Imi-1b1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:17:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 11
            CDSs (with protein)               :: 11
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115733.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed6"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            1..957
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26065"
     CDS             1..957
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_039899550.1"
                     /translation="MAELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTLETPDETE
                     RTVKFSCSEYSSFMGISYENAWGRLNTSTRELFKRSIELIYPTGAVSMRVFNWAEYAEF
                     NRETQEVTLVFSKYIIPLLFHLKRFIKYNLEHVKSFENKYSMRIYEWLLKELTQRKTHK
                     ANISISIDEFKFMLMLEKSYPEYKLLNHWVLKPISKDLNTYSNIKLSIDKRGRPADTLI
                     FQVELDKQIDLVTELAKEPISKKEDNSIRLTPENRLHEGLKTTLHDALTAKIQLTGFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRATLEKILAKYGRN"
                     /name="replication initiation protein"
     gene            965..1414
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26070"
     CDS             965..1414
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_016517644.1"
                     /translation="MALVSISEAARLTGKSRTTVQSYIKQGKLTKCTDSGGASKLDTSE
                     LLRVFGSFTGQQVGSEQSGSTVQHLAGDFVQLPDQNLLHENYALKAEIELVKALLREKE
                     LLLDEKDKRNEDLKHALLLIESKLPNTSEPVTPHVVKKSWQFWKK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1734..2684)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26075"
     CDS             complement(1734..2684)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_26075"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487372.1"
                     /translation="MRKTGMTAQDVINVLTILKANDSTSFSKIQRALKMSISQLEGIID
                     GLTAMGIVYKSSFTSYSLTELTSKPVVSDGVRKAFEDIITNRGTYLSEELLQKVSTPFI
                     PLMTHEYKNAPVKVMIVGQETLGMEDAFSTIVSVDDYINESIESFNKFNFGEDLRNSHF
                     WYAFDEVVKYFNLPSRRHAYWTNLHKFQLIENDGDSVSISKLPSKDIMTMIHMQRELFL
                     AEIKDTKPDIIIYFTGGQTWVLDHYLNNGKKLAVKAIDERSHLGIIQTEFLHCPIAICT
                     DHPGRRGYTQAIVDHRANLLKYSADKFHASESARV"
                     /name="hypothetical protein"
     gene            3203..3346
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26080"
     CDS             3203..3346
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_26080"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_200442548.1"
                     /translation="MIDENEDVRLVGIKFFANSQMDAFVSDMQNRLNEGESLETASLSL
                     PL"
                     /name="hypothetical protein"
     gene            complement(3385..3969)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26085"
     CDS             complement(3385..3969)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(3982..>4158)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26090"
                     /pseudo=""
     CDS             complement(3982..>4158)
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxation protein"
                     /name="relaxation protein"
     gene            complement(4293..7286)
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_26095"
     CDS             complement(4293..7286)
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909877.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_272487373.1"
                     /translation="MPVGFLTPEQRDYFGRYVGSPSREELERFFYLSDEDRDVIQTLRG
                     DHSRLGYAILLTTVRFLGVLPDKPTSVPSEVQQVLVRQLSITDPDCLLRYSDHRRWIHA
                     ADIQTRYGYRHFTDHDVGFRLCRWLYALCWTGTDRPGVLFERATAWLLTQKVLLPGVSQ
                     LERFVAQLRSRVEERLWITLGRSVTEQQRQRLLKLLTVEDGSRGSKLDKLRSGPVMISG
                     PALVKALHRLDDVRSFGITLPAAAHIPPSRIATLARFANTAKVTAISRLPPARQLATLV
                     AFAVCLEATAHDDALDVLESLLRDLFSNAEKADKKARLRTLKDLDRSAATLAAACRMVL
                     DASISDSTLRTQLFANLPRVYLEHALKEVDALIRPANDVFINALEERYRGVRRFLPDLL
                     ERLHFGANPTGKAVVDGFEWLRKNLKCKHPEIDAPQDVVGKSWQKYIIGKDGTLDMRAY
                     VFCVLDALRTAIRRRDVFVSPSWRYADPRIGLLDGPEWIAARPIVCRSLGLSVEAKPTL
                     DAFITELDTTWQAVAKRLPENPAIQLTETDEGKTELSLAALERLDEPESLLALRTAVAN
                     LMPRVDLPEILLEVAARSGFSSAFTHVSERNARADNFATSLCAVLLGDACNTGLEPLIR
                     SDIPALRRDRLSWVGQNYIRDDTLSAANVILVSMQSQLELAQIWGGGEVASADGMRFVV
                     PVRSVHSGPNPKYFGSSRGVTWYNLISDQFSGLNAITVPGTLRDSLVLLAVVLEQQTEL
                     QPTQIMTDTGAYSDVVFGLFRLLGYHFSPRLADVGGTRFWRSSPDADYGQLNGLAKQSV
                     KLELITEHWDDLLRLAGSLKLGRIPATGIMRTLQTGDRPTRLAQALAEFGRIEKTLHML
                     TYINDESKRRATLTQLNRGESRHSLARAVFHGKRGELRQRYREGQEDQLGTLGLVVNMI
                     VLWNTIYMTEALKQLKRQGYQILDDDVARLSPLGWEHINMLGRYSFAVPEEVARGELRP
                     LRNPAEDL"
                     /name="Tn3 family transposase"
     gene            complement(7290..7700)
                     /locus_tag="PGH06_RS00040"
                     /old_locus_tag="PGH06_26100"
     CDS             complement(7290..7700)
                     /locus_tag="PGH06_RS00040"
                     /old_locus_tag="PGH06_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150546.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA];
                     GO:0004521 - RNA endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="WP_023356272.1"
                     /translation="MRVVLDTNILFSALISPHGAPDRIYRAWRTARFEIVTSLVQLDEI
                     RRASRYPKLQAVLQPAKVGTMLNNLQRAIVLERLTIEHEADDPDDAFLLAMALAGDADY
                     LVTGDRRAGLLQRGHIGRTRIVTPAIFCAEAL"
                     /name="putative toxin-antitoxin system toxin component, PIN
                     family"
     gene            complement(7700..7939)
                     /locus_tag="PGH06_RS00045"
                     /old_locus_tag="PGH06_26105"
     CDS             complement(7700..7939)
                     /locus_tag="PGH06_RS00045"
                     /old_locus_tag="PGH06_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="WP_023356273.1"
                     /translation="MQTTRWNVAVSASTDQALRMFLASQGQGKKGDLSRFIEDAVQAHI
                     LELTAVQAKTANAHLDEAEITSMVDEALDWARHR"
                     /name="ribbon-helix-helix domain-containing protein"
     gene            8152..9072
                     /locus_tag="PGH06_RS00050"
                     /old_locus_tag="PGH06_26110"
     CDS             8152..9072
                     /locus_tag="PGH06_RS00050"
                     /old_locus_tag="PGH06_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150544.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_022631165.1"
                     /translation="MKIGYARVSTKDQTVDLQLDALKQAGCERIYQEVASGAKTARPEL
                     DRLLVDIRAGDTIVIWKLDRLGRSLKHLVELVGKLAERKIGLLSLNDPVDTTNAQGRLV
                     FNLFASLAEFERELIQERTLAGLSAARARGRVGGRPKGLSAPAESTAMAAETLYREGRL
                     SVSAIGKKLHISKGTLYRYLRSRGVEIGKQKKNQLTDTLQPTAINGSPITKTATVSLLF
                     SVENNSKFVRGKKRAQANIEQYSLALYDNKQLAPGKYELRIPYENEHELNETMHQLLSD
                     MNREADLCNCNVDANAWEEGTERRW"
                     /name="recombinase family protein"
     gene            complement(8979..10037)
                     /locus_tag="PGH06_RS00055"
                     /old_locus_tag="PGH06_26115"
     CDS             complement(8979..10037)
                     /locus_tag="PGH06_RS00055"
                     /old_locus_tag="PGH06_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_272487371.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNARVSDAMKDIGVWGAMEPKTNVRVYHRRSVPSSHAFASTLQL
                     HKSASRFISDRS"
                     /name="MobA/MobL family protein"
     gene            10202..10669
                     /gene="traD"
                     /locus_tag="PGH06_RS00060"
                     /old_locus_tag="PGH06_26120"
     CDS             10202..10669
                     /gene="traD"
                     /locus_tag="PGH06_RS00060"
                     /old_locus_tag="PGH06_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_064645523.1"
                     /translation="MNKSEKQEKWAADRVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSQTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSSA"
                     /name="traD"
     Typing          2..952
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.376"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(9115..10037)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.377"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctgaat tggtagtatt caaagcaaac gagcttgcgg ttagtcgtta tgacctaaca
       61 gagcacgaaa cgaaactaat cctgttttgt gttgcaaagc taaaccctac attagaaaca
      121 ccagatgaaa ctgaacgaac ggttaaattt tcatgctccg aatattcttc atttatgggg
      181 ataagttatg agaatgcgtg ggggcgatta aatacatcaa cacgagagtt atttaagcgg
      241 tctattgaat taatatatcc aacaggtgct gtatctatgc gtgtttttaa ttgggctgaa
      301 tatgcagagt tcaataggga aacacaagag gtaactttag tttttagtaa atatataatc
      361 ccattactat ttcacctaaa gagattcatt aaatacaacc tcgagcatgt taagtccttt
      421 gaaaataaat actcaatgcg aatttatgaa tggctgctaa aagaactaac gcaaagaaaa
      481 acacataaag ccaatatttc aataagcatt gatgagttta aattcatgct aatgttagaa
      541 aaaagctacc cagaatataa attactaaat cattgggttt taaaaccaat ttcaaaagat
      601 ttaaatacct atagcaatat aaaactatct attgataagc gcggtcgtcc tgctgatacg
      661 ctgatcttcc aagttgaact ggataaacaa attgaccttg tgactgagct agcaaaagaa
      721 ccaatatcaa aaaaagaaga taactcaatc cgtttaacgc ctgaaaatcg tctgcatgag
      781 gggctaaaaa caacattaca tgatgcttta actgcgaaaa tacaacttac tggatttgaa
      841 gctaaattcc tgagtgacat gcagagtaag tatgatctca acggctcatt tacatggctg
      901 actcaaaaac aacgagccac gctagagaaa attttagcta agtacggacg gaactgaggt
      961 atctatggct ttagtatcaa taagcgaagc agccagacta actggaaaaa gccgcacaac
     1021 agtacagtca tacattaagc agggaaaact gaccaagtgc acagatagcg gtggtgcttc
     1081 aaaactagac acatcagagc ttctacgtgt tttcggatca tttactggtc agcaggttgg
     1141 cagtgaacaa tcaggcagta ctgtgcagca tttggcaggc gattttgtgc agttacctga
     1201 ccagaatttg ctacatgaaa actatgcctt aaaagctgaa atagaactcg taaaggcatt
     1261 gctgcgtgag aaagagctac tactcgatga aaaagacaaa cgcaatgagg atctcaaaca
     1321 tgctttgctg ctaatcgagt ctaaactgcc gaatacatcg gaaccagtga cacctcatgt
     1381 ggtgaaaaaa tcatggcaat tctggaagaa atagcgcttt cagccggcaa accggctgaa
     1441 gccggatctg cgattctgat aacaaactag caacaccaga acagcccgtt tgcgggcagc
     1501 aaaacccgta cttttggacg ttccggcggg tttttgtggc gagtggtgtt cgggcggtgc
     1561 gcgcaaggga tgttatggta aattgaatcg ccacggataa tctagacact tccgagccgt
     1621 tgataatact agttttaata ttctgtcggt gacatgtgtt cgctagaacc atgccgacgt
     1681 tactgttata aaacatttcg atgtaagcga tcttaaatga ctctgggggg ctgttaaacc
     1741 cgtgccgatt cacttgcatg aaatttgtca gcagaatatt taagcagatt agctctatga
     1801 tctacgatgg cttgagtata tccccttcta ccagggtggt cggtacatat agcgattggg
     1861 caatgcaaaa attctgtctg gataatcccc agatgcgagc gttcatctat tgccttaact
     1921 gctaattttt taccattgtt tagataatga tctaacaccc aggtttgccc acccgtgaag
     1981 tagatgatga tatctggctt ggtatcttta atttcagcca agaataattc acgctgcata
     2041 tgaatcattg tcatgatatc ttttgaaggt agtttggaaa tgctgacact atctccatca
     2101 ttttcaatta attgaaactt atgaagatta gtccaataag catgccttct ggatggtagg
     2161 ttaaaatact ttactacttc atcaaatgca taccaaaagt ggctattcct taaatcttca
     2221 ccaaaattga atttattgaa ggattcaatg ctctcattaa tgtagtcatc gaccgaaacg
     2281 atggtactga aagcatcttc cattcccaga gtttcttgcc ccacgatcat cactttaact
     2341 ggggcatttt tgtattcgtg cgtcatcagt gggatgaatg gtgtacttac cttttgtagt
     2401 agttcttctg acaggtaagt accgcgattg gtaatgatat cttcgaatgc ctttcttaca
     2461 ccgtcactaa caacaggttt agaggttaac tcagtaaggc tgtatgaagt gaatgatgat
     2521 ttatacacta ttcccattgc agttaaccca tcaatgatgc cctccaactg cgaaatactc
     2581 atctttaacg cgcgttgaat cttgctaaaa ctggttgagt cgttggcctt aagaatagtg
     2641 agaacgttga ttacatcttg tgctgtcatt cctgttttcc tcatattgaa ctgcttcaat
     2701 gtagatacgc gtttaactca ttctgacggt cgcagtacaa aaaattagtc aaaaatcaca
     2761 tgccccggta atccctggat tggcccctat atttctctaa tgaaccggca tcaataacga
     2821 ctttgcctga ttggttataa gctaaaaatc ctcagagata aacgactata accgagcatt
     2881 gcttcaagac atcgaccgta gtggcctatc atgattttta tgattggggt cagtttggag
     2941 atcggaaatt tttgtacgtt aagcgcatta tttaaccaat cctggttcgt aaggggttga
     3001 ttttgcgtca gtggctatcg cttttaagtt gtcgctcttg cagtatcgca tttaggttat
     3061 ccgattaagg tcaaacctct gaaaatgccg tatagcgcgg gagtacaccc tattgctata
     3121 ggtaagtctg ttcaaaaaac aggcttaccg tacaataatt ctctatatcc aaactgaccc
     3181 cctgaatgac agggagcgca ttgtgattga tgaaaatgag gacgtcaggc tggtcggaat
     3241 taaattcttt gccaacagtc agatggacgc cttcgtcagc gacatgcaaa accggctgaa
     3301 tgagggcgaa tcgctggaaa ccgcttcgct ctccctgccg ctgtaattca cgacaacaat
     3361 aacggcgcaa tgtgcgccgt tatgttattt tttgataatc atccaggtat tctctttatc
     3421 accatacgta gctcgggtgt ccaccttaac gcagggcttt ccgtcacaac gcgatatatc
     3481 agccagcggg gctttcttct gccagaccgc ttccatccgt tgcatttcag caatctggct
     3541 gtacccgtca ttcataaacc acgtaaatgc cgtcacgcag gcagccatga cgaaaaatat
     3601 cgtcagcacg agataaatcg cggatttcca cgtatagcgc gacatctcgc gtcgcatttc
     3661 atggatcatc gccttcgccg tatcggtagc ctgattaagc gcttctgtcg ccggtttctg
     3721 ctgtgctaat ccggcttgtt tcagctcttt ctcaacctga atgagcgcgg catgaacaga
     3781 ttgcctgacg gtttcagtca tattaccgga cgcgctgtcc agctcacgaa tgagcctgct
     3841 cagcgtgtca ctctgctgct gtaattgtgc tgaggcgtcc tgaaactgct cagtcagaga
     3901 ggtaacacgc ttttccagcg cctgatgatg cccgatgagg gcagcgattt gtttaatttc
     3961 gtcgctcatg aaaaatcctg tctatcgtga aaatgaccag cctttatccg gcttctgtcg
     4021 tgtctgttcg gcgagtctct gctgttcttt ctcctgctga cgctgttttt ctgccagtcg
     4081 ctccatttcc tgatgtatcc cctgaaactc cgccatcgca tcgttcacaa gggactgaag
     4141 gtcgatttct tcctgtatgt ggggtttagg gagcaatgga accaaaaacc aacgtaagaa
     4201 aaatatccgc acatttcatc gaatagctct gaagtccagc ggtacggaaa cgtacgctag
     4261 caagaggcat cattctgaat ggatgtactg ccctataggt cttcggctgg atttcgtagc
     4321 ggtcgaagct ctcctcttgc aacttcttcc ggtacagcaa atgaatatct tcctaacata
     4381 ttgatatgtt cccagcccaa gggggataac ctggccacat cgtcatcaag gatttggtaa
     4441 ccttgtcgtt tgagttgttt caaggcttcc gtcatataga tcgtgttcca caagacaatc
     4501 atattgacca ccaaacccag cgtacctaat tgatcttcct gtccctcacg gtaacgctgg
     4561 cgaagctcgc ctcgtttgcc atgaaaaacc gcccgcgcca aactatgccg actttctcct
     4621 ctatttaatt gcgtcagcgt agcgcgccgt tttgattcat cattaatgta ggtcagcatg
     4681 tgcagtgttt tttcgatccg gccaaattca gccaaagctt gcgccaatcg ggttgggcga
     4741 tcaccggttt gcagagtacg cataatgccg gtcgcaggta ttcgtccaag cttcagtgaa
     4801 cccgctaaac gtaatagatc atcccaatgt tctgtgatca attccaattt aaccgattgt
     4861 tttgccagtc cgttgagttg gccataatcg gcatcggggc ttgatcgcca gaatcgagtt
     4921 ccccccacat cggccagtcg aggactaaag tggtaaccca gtagacggaa tagcccaaag
     4981 actacgtcgc tgtatgcgcc ggtatcggtc atgatttgtg taggctgtaa ttcagtttgt
     5041 tgttccagca caacggccag caacaccaga ctgtcccgga gcgtgcctgg cacagtgata
     5101 gcattcaacc ctgaaaactg atctgaaatt aagttgtacc aggttacccc tcggctggag
     5161 ccaaaatatt ttggattggg gcctgaatgt actgagcgca caggaacgac aaaacgcatt
     5221 ccatcggcag atgcgacttc accgccgccc catatttgag ctagttcaag ttggctttgc
     5281 atcgacacca aaatcacatt ggcagccgat aaggtatcat cacggatgta attttgaccg
     5341 acccatgata aacggtcacg tcgtaaggct gggatatccg accggatcag cggttccagt
     5401 ccagtattac aagcatcacc cagtaatacg gcgcacaaac tggtagcaaa gttatcggca
     5461 cgggcattac gttcagatac atgcgtaaag gcactactga agcctgaacg cgctgcgact
     5521 tccagcaata tttccggcag atcgacacga ggcatcaaat tagccactgc cgtgcgcaat
     5581 gccaataaag attcaggttc atccagtcgc tctaaagcgg cgagtgataa ctcagtttta
     5641 ccctcatcgg tttctgttag ctgaatcgca ggattctccg gcaagcgttt tgcgaccgcc
     5701 tgccaagttg tatccagttc tgtaatgaat gcatccaaag taggctttgc ttcaacgctc
     5761 aggcccagag atcgacacac aatcgggcga gcagctatcc attccggacc gtccagcagg
     5821 ccaatgcgtg gatcggcata acgccaactg ggtgaaacaa acacatcccg ccgccgaatg
     5881 gccgttcgta gcgcatcaag cacgcaaaat acataggctc gcatatccag cgtaccatct
     5941 ttgccaatga tgtacttttg ccatgacttt cccactacat cttgtggcgc atcaatttca
     6001 ggatgtttgc acttcagatt ttttcgcaac cattcaaaac catcaaccac agcttttccc
     6061 gtgggatttg caccaaaatg caaccgttct aacaggtcag gcaaaaatcg acgaacacct
     6121 cgataacgtt cttccagagc attgataaat acatcatttg cgggacgaat aagcgcatcg
     6181 acctctttca atgcgtgttc taaatagaca cgtggcaagt tggcgaataa ttgcgtgcgc
     6241 aaggtgctat cactaattga tgcatccaaa accatccggc aagccgctgc cagtgttgca
     6301 gcagatcgat ccagatcttt cagtgttcgc aggcgagctt ttttatccgc cttttctgca
     6361 ttgctaaaaa ggtcgcgtaa cagggattct aaaacatcca gtgcgtcatc atgcgcggtg
     6421 gcttcaagac aaaccgcaaa tgcaaccagt gtggccaact gtcttgccgg tggtaaccga
     6481 ctgatagcgg tcacttttgc cgtattagca aatcgcgcca gagtggcaat tcggctgggt
     6541 ggaatatgtg cagcggcggg caaggtgatc ccgaagctac gcacgtcatc cagtcgatgc
     6601 agtgctttaa ccaatgcggg gccactgatc atcacggggc cagaacgaag tttgtctagc
     6661 tttgaaccgc gactaccgtc ttcaacagtc aatagtttca acagacgttg gcgctgttgt
     6721 tcagtaacgc tacgtccaag cgtgatccaa agacgctcct cgacccgact ccgtaattga
     6781 gcgacaaaac gctctaattg agaaacacca ggcaatagaa ctttttgtgt cagtagccag
     6841 gctgtagctc gttcaaataa tacgccaggc cgatccgttc ccgtccagca gagggcatac
     6901 agccaccgac acaaacgaaa accaacatca tgatcggtaa agtggcgata accatagcga
     6961 gtttgaatgt cagccgcatg tatccagcgg cggtgatcac tgtagcgcag tagacaatca
     7021 ggatcggtaa tggagagttg gcgaaccaag acttgttgga cttcggaagg tacactcgtc
     7081 ggtttatcgg gcagaacgcc aaggaaacgg actgtcgtca ataatatggc ataacctaat
     7141 cttgagtgat cacctcgcaa ggtttggatg acatcacggt cttcatcact caaatagaaa
     7201 aaacgttcca attcttcacg actgggcgaa ccaacataac gaccaaagta atcacgttgc
     7261 tctggagtca gaaagccaac cggcatagat cacagcgcct ccgcgcaaaa aatggctggg
     7321 gtcacaatcc gtgtccgccc aatatggccc cgttgcaata aacccgctcg tcgatcacca
     7381 gtgaccagat aatccgcatc accagccaat gccatcgcta acaaaaaagc atcatcagga
     7441 tcatcggctt catgctcaat tgtcaatcgc tccaaaacga tggcccgttg caaattattg
     7501 agcatggtcc cgactttggc tggttgcaaa actgcctgta gtttagggta tcgactggct
     7561 cgacggatct catccagttg taccagtgag gtgacgattt cgaaacgcgc agtacgccag
     7621 gcgcggtaaa ttctatcggg tgcgccatgt ggcgaaatca gcgcactgaa cagaatattg
     7681 gtatccagaa caactcgcat taacgatgac gagcccaatc aagtgcttca tcaaccatgg
     7741 aagttatttc agcttcatcc aagtgtgcat tggctgtttt ggcctgtacg gctgttaatt
     7801 ccaaaatatg tgcttgtacg gcatcttcga tgaagcgcga tagatcacct tttttccctt
     7861 gtccctgact ggccagaaac atgcgtaaag cctgatcggt cgaagcagag acggcaacat
     7921 tccagcgagt tgtttgcata gtctattttc tcaatgtgtg tttatgcgtt tatacaccca
     7981 aatatttaac tatgcaatag ttctataaaa cggaggtttt atggaacttt aatggtaatg
     8041 ataaatatgc tctggtttgc aaagtttata gcgcaataag gtataaaata ctggttctta
     8101 aaatcaaagt tcaactattc tgaatatagt tgagtaaatg gaactttatt gatgaaaatt
     8161 ggctacgcac gagtgagcac taaggaccaa accgtagatc tacaacttga tgcactcaaa
     8221 caagcaggat gtgaacgcat ctatcaagag gttgccagcg gagcaaaaac ggcaaggccg
     8281 gaattggatc gtttattagt ggatattcgt gctggcgata cgatcgtgat ctggaagctg
     8341 gatcggcttg gccgttcgct taaacacctg gttgagctcg tgggcaaatt ggccgaacga
     8401 aaaattggcc tgctcagtct gaatgatcct gttgatacta caaacgccca aggccgtttg
     8461 gtctttaatc tgtttgcatc cttggcagag tttgagcgtg aattaattca agaacgcaca
     8521 ctcgcggggt tatccgccgc acgagcaaga ggtcgggtag gcggaaggcc caaaggttta
     8581 tctgcaccgg cggaaagtac cgcaatggcg gctgaaacgt tatatcgcga agggcgactc
     8641 agtgtcagcg cgattggtaa aaagctacat atatccaaag gcacgctcta tcgttattta
     8701 cggtcacgag gtgttgaaat tggtaagcag aaaaagaacc agttaacgga tacgttacaa
     8761 cccacagcaa taaacggatc tccgatcacc aaaacagcca cagtaagctt gctgttcagc
     8821 gttgagaaca atagcaaatt tgtcagaggt aagaagcgcg cccaggcaaa catcgaacaa
     8881 tacagcttgg ctctctatga caataaacag ttagcgccag gaaaatatga gttgcgcatt
     8941 ccctatgaaa atgaacatga attgaatgaa acgatgcatc aacttctatc agatatgaac
     9001 cgagaagctg atttatgtaa ttgcaatgtt gatgcaaatg catgggaaga agggactgaa
     9061 cggcgatggt agactcttac gttggttttt ggttccattg ctccccaaac ccctatgtcc
     9121 ttcatggcat cactgacccg tgcgttcagt ttctccggct ctttctcaaa atcgaaaacc
     9181 ctgccggaag tgtcttcctg cgcaggttca ggcttctgct cttgttttat cgtcaggtta
     9241 tcccgctcgc tgatggcttc ccgtagcgag gtggtcacgt caatcacact gtcacgttca
     9301 tcacgggact gctccgcctg cctttcagcg tccctgcgct caaggatggc ctgtaactgc
     9361 gcagtatcga accgctgaac ctgtcccgcg ccaagatgcc gctcaggctc acgctcaatg
     9421 ccctgagctt tgagtgagcg tgcatcaacg cggtcggtat gctgataccg ttcaaggtgc
     9481 ttattctgta ggtcagccca gcgttcccgc tgggcgacga ggtgctcttt gcgttctgtc
     9541 ggcgtttccc cgaaacgtgc ctttttcgca ccgccgcgct caggggcttt gctgttagct
     9601 cgcttaaaat actgctgcgg gtcacggtcg ataccgtcat tcatccgctc agagaacatg
     9661 atatgcgcgt gcggctgctc gccgccggct atcgccgctt tcggattatg aatggcgaac
     9721 tgatacgcat gacggtcgcc gatttcctgc cggacaaaat cgcggacaag ctcaaggcgt
     9781 tgttcgggtt ttagctcgcg cggcagggca atctcgattt cacggtaggt acagccgttg
     9841 gcacgttcag acgtgtcagc agctttccag aactcagacg gtttatgcgc tgcccacgca
     9901 ggcatattac cggactcctt gtgctcaagg tcggagtctt tttcacgggc atactttccc
     9961 tcacgcgaaa tataatcggc atggggagag gcactgcctt tcccgccggt ttttacgctg
    10021 aggtgatagg aggccatcgt gtttttatcc cgctgaaggc gcgcaccgtt tctgaacgaa
    10081 gtgaagaaac gtctaagtgc gcactgataa taaaaagctt atcagtgatt ctacctgcat
    10141 ttgacctcgg ccgccattcc tgaccataat ttccacatca acattcagag gataaatggg
    10201 catgaataaa agcgaaaaac aggaaaaatg ggcagcagac agagtgcagt acattcgcgg
    10261 cttaaagtcg ccgaatgaac agcagaaact catgctgata ctgacggata aagcggataa
    10321 aacagcgcag gatatcaaaa cgctgtccct gctgatgaag gctgaacagg cagcagaaaa
    10381 agcgcaggaa gccagagcaa aagtcatgaa tctgatacag gccgaaaaac gggcagaagc
    10441 cagagccgcc cgtaaagccc gtgaccatgc gctgtaccag tcagccggac tgcttatcct
    10501 ggcgggtctg gttgacagcc agacggggaa gcctgttgat gataccgctg ccttgctggg
    10561 tgcattagcc agtctgaatg acctgtcgag ggataatccg aagtggtcag actggaaaat
    10621 cagaggtcag gaactgctga acagcaagaa gtctgacagc tctgcatagc agacctgcca
    10681 taaaacgccc tgagaagccc gtgacgggct tttcttgtgt catgggtaga tttcctcgtg
    10741 tgagtccatg aaaagcgcct gtagcatcat ttacccccat tccctgccag agccgtgagc
    10801 gtagcgaact gaatgtcacg aaaaagacag cgactcaggt gtctgatggt cggagacgaa
    10861 aggaatattc agcgatttgc ccgagcttgc gagggtgcta cctcattctt tcgtgtttga
    10921 aggtatgttt tttggaggag caaacgctgt ttgcgacatc cttttatcat atcgctcaac
    10981 tggcactggc ggttagttat ccacaaagct gggatctatt cttttttatc ttttttattc
    11041 tttctttatt ctagtgattt taatattttg ttttataagt aaaaaacaca tgaaaggtta
    11101 agtgaaaatt aggaaaggtt aagcaaaaat taggatctcc tttcaaaagg ttaagtgaaa
    11161 attaggtaat ccacacaacc ccataaaagg ttatgacaat gaaattgaca taaccaagcg
    11221 gaagggttat gttgttttaa aatcacaacc aatggtttta gtc
//
