LOCUS       NZ_CP115727             4127 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed12, complete
            sequence.
ACCESSION   NZ_CP115727
VERSION     NZ_CP115727.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                :: C_braakii_GW-Imi-1b1
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/09/2024 02:18:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115727.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed12"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            complement(481..1224)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25350"
     CDS             complement(481..1224)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487361.1"
                     /translation="MVTRRYLLGGEYPSEEFVQASLEKYFSRQGFDIDTGSYIDLICRD
                     KESRDVVWHIEVKGKTSQPGLDFRTCLGQLVQRMTKDNINYAIAVPRIKQYERLIEETS
                     ILAVSKLNLHWIFVGPDGSVTIKEPDSSTMKNGNGNGNGNGNGNGNGREVSLVNGGFKR
                     FITGDFMPTCSYGKAPVVFKSSRREMWDWLDDFVISNGVIPSIADIKEEAESRSGKWTL
                     SNVKIEYQFWKIYHLYKGHFFYQSE"
                     /name="hypothetical protein"
     gene            complement(1304..1603)
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25355"
     CDS             complement(1304..1603)
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023281092.1"
                     /translation="MATATNFDAWLDDVDGDYEEVMALYDSVQNVSDMGLYQCVEGGRG
                     DAWVVSSNHHPEALFLASAVARDTFLKLIRERLCGGEDVDSWYGFQRNISNDHS"
                     /name="hypothetical protein"
     gene            complement(2453..2875)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25360"
     CDS             complement(2453..2875)
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_172696149.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKHDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2875..3066)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25365"
     CDS             complement(2875..3066)
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3546..3695)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_25370"
     CDS             complement(3546..3695)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_166479530.1"
                     /translation="MPSEYDEQRDATDAPLPGEYDVFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     Typing          complement(3689..3905)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.758"
                     /coverage="100.0"
                     /evalue="9.55e-62"
     Typing          2..453
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.047"
                     /coverage="98.0"
                     /evalue="1.05e-134"
     Typing          3999..4127
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.615"
                     /coverage="85.0"
                     /evalue="1.24e-48"
ORIGIN
        1 ctccctcgtg cgctctcctg ttcctgccat tcggtttacc ggtgtcattc cgctgttatg
       61 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
      121 tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgcctgttcc ggtaactatc
      181 aacttgagtc caacccggaa aggtacgaca aagcgccact ggcagcagcc actggtaaac
      241 aggatgtgcg agagatgtat ttgagttctt gaagtgaaga cctaactgag gctacactgg
      301 aaggacggat ttggtgacat gtctcgcaca agactgttac cacggttcat aaacttgccg
      361 gttaatgaac cttcgaaaaa ccaccttgcc gggtggtttt ttcgttttcc gagaaagaga
      421 ttacgctttg agcgaatgaa tctcaagaag atcatggcat ttgatgaaca aatgagtcag
      481 ctactcagat tgatagaaaa aatgcccctt atacaagtga tatattttcc aaaactgata
      541 ctctatttta acattagaaa gcgtccattt cccactccgc gattcagcct cttctttaat
      601 atcagcaata cttgggatca ccccatttga aataacaaaa tcgtccaacc aatcccacat
      661 ttctcgccga gatgatttaa atacaacagg agctttccca tatgagcaag ttggcataaa
      721 atcccccgta ataaatcttt taaacccccc attcacaaga gaaacttctc ttccattacc
      781 attaccatta ccattaccat taccattacc attaccattt ttcatcgtag agctatcagg
      841 ttccttaatt gtcacactgc catcaggccc aacaaatatc cagtgtaaat tcaatttact
      901 aacagcaaga atgctcgttt cttctattaa tctttcatac tgttttattc ttggaacagc
      961 aatagcataa ttaatattat cttttgtcat tctctgcact aattgtccaa gacaagttcg
     1021 aaaatccaac cctggctgtg atgtttttcc ttttacttca atatgccaca caacatccct
     1081 agattcttta tcacgacaaa taaggtcaat ataagaaccc gtatctatat cgaacccttg
     1141 tcgagaaaaa tatttttcta aagaagcttg aacaaattcc tcggagggat attcaccacc
     1201 tagtaagtac cttctcgtca ccataagagc cacaacccct acgcatccta tttactataa
     1261 cgtcatattt taagagttcc cacaagggga actctctgta cgattacgag tgatcattcg
     1321 atatatttcg ttgaaatcca taccatgaat caacatcttc gccgccacat agcctttctc
     1381 taatcaattt gaggaaagta tccctagcca cagctgatgc tagaaaaaga gcttctggat
     1441 gatgattaga gctcacgacc caagcatcac cccgaccacc ctcaacacac tgatacaacc
     1501 ccatatcact tacattctga acggaatcat aaagagccat aacctcctca taatcgccat
     1561 caacatcatc taaccacgca tcaaaattag tagcagtcgc catgccaatc ctccattttt
     1621 tagttttcag gagttaattt acatagtgat aggggctgtt aagcaaataa gtttttaccc
     1681 tttttgcctc atgctctaaa agtgagcaag acaggcattt accataacgt cgcattacac
     1741 gcaccgctca aacgctcgag aaacgattct ggggcgtaac ccattaaatc ctacgtctta
     1801 ccccctgaaa acgcctcaaa atcgcgctga tgggttttac gggcatgaat tgttatgcac
     1861 cagactggct ggaggtagat ttcatcctga attcacgcca gacaactcaa aacccgcacc
     1921 aaacccgact gaaaacggtg aacggggacg caatttattg cgtggccgtg aagcgttgcc
     1981 ggcgcagccg gaaaaccggt tttcagcacg ttcaggaatc gggttcgtcg acggccggca
     2041 atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga ttcgactcgt
     2101 gacttgactc tagagtcacc acggtcaaaa aaacgccaaa gcgacgattc gactctagag
     2161 tcaagtcgtc ataagttact gatagtgtgg agtgagtcgt cgatgactct atgacgagtc
     2221 gtcggcaagt cgtacacgaa tcaccgacga cttggattga ccttacagaa ggtaatactg
     2281 cgtaatttaa taacaaccaa ctgactgata ttaatgagtt atctgcaaaa atttaatttt
     2341 gcgtggggtg tggatatttt tagtgtgggg agcgttcaga gatctggcga tttttgggaa
     2401 ggttcacgag cgccccggca tggccggaga ggaaaactca agcatggcgg gatcaggatt
     2461 ttttccagaa tttccaccac ggggaatggg gtggtgaaga aggttcgtgc ttgtgctcca
     2521 gaagctgcat agcctgacga agggaatcaa tatgctcatc tcgagcctta acagcccctt
     2581 taagaccatc gttttcagct tcaagtaaag cgattctatg ttgtaaatca tgtaaaccaa
     2641 cgctgttaat acctgtagat tctgcgtcac tttttacatc atgttttaca gcatcgatta
     2701 caccatcaag agaaccaaac actctaataa gctctgatgt atcaataact ttaataccct
     2761 tcccgtgctt tacaacagag gtgtaactta acttaccact gttaatcatc ttgtaaatag
     2821 tagggcgact aaccttaaca agccgtgctg cttcactaat agacactctg gccatcagtt
     2881 accatcctgt aatgcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc
     2941 gtggagtttc tcacaaaggt cggcctcagc atcgagatca agctcgttaa gcttctccag
     3001 caggagcaga gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt
     3061 cgtcataagg gccctcagag catctgtgct cagtgtaaac cggtaagggg gtcggatgta
     3121 caaatggttc agagaacgga tctgaggtaa tcctgtagtt attttgtagt ttatctgtag
     3181 tcatcgcact aaaaatcagg attaaatcat caacttttgt agtcttcctg tagtttgcct
     3241 gtagtccgga aaaataatcc gttcatgcaa gcatgaacga agcagttaaa gacgcttttt
     3301 cgtcaccaat gctccttaac ctgcgcagta caccctgcca gaatgataat tttttgcccc
     3361 ttaaatggtc atacacctaa cgcagccgcg attttcatcg caactgcatg aattaaaagg
     3421 taaaaactga aatctccgtt tattcatgta atacgcaacg tattacaatc acctgtaatt
     3481 acatgaaaat aaagaaataa aatgtaatac cggcgtatta ccgcgtacgc gggcaagcga
     3541 aaaagctata acgcttcccg gccgacaggc ggcccggaat ccacaggggc gtgcaggttc
     3601 tcttcagtaa cctgcgccat taaatcaaaa acgtcatatt cgccgggaag cggagcatca
     3661 gtcgcatccc gttgttcatc atactcagat ggcatggggt gatatccagt agctgagaga
     3721 ttgttagcgg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
     3781 ctggcttaat atgttacgga tgagtaacac gtaatgaaag tgcttcatgt ggcaggggaa
     3841 aaatgcagcg gcagggcgta caggagaaaa tggaggttac ggtgatatga cgcctcctcg
     3901 ctcactcggt cgctgacgct cctgccgtga gtctgcggcg ggcgttacct gcacacggat
     3961 aagagcaaag acatacggca tgacaagcgg ggcagcaaaa ggctgcggag ctgccgtttt
     4021 tccataggct ccgcccccct gacaagcatc acgaaaatct gacgctcaaa tcagtggtgg
     4081 cgaaacccga caggactata aagatcccag gcgtttcccc ctggtag
//
