LOCUS       NZ_CP115726             4593 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed11, complete
            sequence.
ACCESSION   NZ_CP115726
VERSION     NZ_CP115726.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                :: C_braakii_GW-Imi-1b1
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/09/2024 02:15:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115726.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed11"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            40..867
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25310"
     CDS             40..867
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_272487398.1"
                     /translation="MPVKPYCSDDLSTGLRIRKKEKALEMLYIQANQPAIQTCLLFDLD
                     DDNCFYKFEDVGLPVPHFITKSPNSGRCHYGYILQNGVCKTQQARLKPLRYAAAIENGM
                     ANKLGSDPGYVGLITKNPLNPHWSPYWSGADLYDLDYLADFVDLNAPNPKEKRGLEYGL
                     GRNVNLFDDLRLYAYRHVLEFKDSSGFDSWMREIENQAINLNTLCNPKNLLPFGEVKAT
                     ARSIGKWTWKNFSRSAFSSVQAHRGRMNGGKKKNNYKKQSNIEEMIRFLGGEK"
                     /name="replication initiation protein"
     gene            864..1133
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25315"
     CDS             864..1133
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="WP_128318011.1"
                     /translation="MRVSRNGLTQKQLAEKFNISVTTVIKYTAIEREEYESKAKERRLK
                     AYTMRSNGFSLKEIALELGCSYNAAAALVKRYKHIDMLKNHSVV"
                     /name="plasmid replication protein"
     gene            1169..1327
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25320"
     CDS             1169..1327
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164876883.1"
                     /translation="MNLETLYHHDLKEKKETDFSFSIEERKWAAEAVADCYKRIENGTA
                     KKLKARD"
                     /name="hypothetical protein"
     gene            1332..1649
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25325"
     CDS             1332..1649
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128318012.1"
                     /translation="MYSVITANNTLDEFVEEYLSDDDFNQLIRKLENMPHTRPYWENTR
                     YKYIIAAKIYFLYEIDDSNMYVFILAAKFNNSRKTFNFAKTPTQIKTMKKWIKNKKKIY
                     Q"
                     /name="hypothetical protein"
     gene            complement(1725..3116)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_25330"
     CDS             complement(1725..3116)
                     /locus_tag="PGH06_RS00025"
                     /old_locus_tag="PGH06_25330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272487400.1"
                     /translation="MIHKQIGGKKRQKNVEHSLNYILRVRPTDTEENRKFVQVMTTTTR
                     ADIENFNDYIKSKKFAHPYITGVLSFEENDTDENLKQKMIADFEEVLFSGVPPENRPPI
                     MWVQHMDKGRLELNYTTFNALQDGRAFKTYYHNTDHHLFNAFCEKNNFEHGFSSVLDKD
                     EKNSLIRVPTNIPEEKRAKLEQIQNEILGKIIVQEINNRDELIDYLKSKDIHINRVRKN
                     QISIKFSPEDEKPTVLKGDIYEEGRDYNLYREPPKNDRTRDPEFAKRALAEHREIFDKL
                     LKNRSGRIAERYNRPPKKNASIIEPRAKKTNDHEDQNNQHKNTDSFDLDSFSKFVFNDF
                     DNNKNGSEVLNEQNNRNDKIEPREERTRQIEAIDFEQQRINKIAETVRNRQLRNRAEAN
                     TVEFRIGFTRRHFSGFVQFFQKVVRPFFVHTRKDEVREKFEKRREETKRHEKGKQKNEI
                     KRLRF"
                     /name="hypothetical protein"
     gene            complement(3091..3465)
                     /gene="mobC"
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_25335"
     CDS             complement(3091..3465)
                     /gene="mobC"
                     /locus_tag="PGH06_RS00030"
                     /old_locus_tag="PGH06_25335"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_128318008.1"
                     /translation="MRDYKEKYQRKNFVSLCLSDQEMSEVDELTKRLDLTRAAAIRELL
                     LTSTTKLKNRIHKNDDKEQLFLLSNVANNINQIAKKMNTNIDYFLSGNGEQFAYLFDQI
                     VDDIEKIKRGSANDTQTDRR"
                     /name="mobC"
     gene            complement(3669..4268)
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_25340"
     CDS             complement(3669..4268)
                     /locus_tag="PGH06_RS00035"
                     /old_locus_tag="PGH06_25340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128318009.1"
                     /translation="MSFLKSLDKKDAVEQISSTVNLRLTLDEDRLFTIASQNSGYSRNE
                     LTYLLLKRYIFDDTSLKDMANQSDDGLHAGIYPNCYVVNTNIVHDKDDDLFMVSNGIAA
                     TFEDGYKEKMTYIEKGSLVFLYRSGEGIVAHGIASGEVLKKDHYGVPDKTYYQVLSDFV
                     LYDKPLTPADIKKLLGRNIVFMHTWFSLKDGYKLIK"
                     /name="hypothetical protein"
ORIGIN
        1 atgtataaca acgataatca acaaaatttt gtaagtcaaa tgcctgtgaa gccttactgc
       61 tctgatgatt tgagcactgg acttcgtatc aggaagaaag aaaaagcact ggaaatgctc
      121 tacatacaag caaaccagcc tgccatccaa acctgtttgc ttttcgatct cgatgatgac
      181 aactgtttct ataaattcga agatgtcggg cttcctgtac cccacttcat aacgaaaagc
      241 ccgaactcag gaagatgcca ctatggatac atacttcaga acggagtgtg taagacacaa
      301 caggcacgat taaagcctct aaggtatgcc gcagccatag aaaacggtat ggctaacaaa
      361 ttaggatcag atccgggcta cgttggacta atcacgaaaa acccgctaaa tccacactgg
      421 tcaccctact ggtctggggc tgatctatat gatttagatt atctcgccga ttttgtagac
      481 ctaaatgctc ctaacccaaa agaaaagaga gggctggagt atggacttgg ccgaaatgtt
      541 aacctttttg acgatctacg cttgtatgcg tataggcacg ttttagagtt caaagattca
      601 tcaggttttg actcttggat gcgagaaata gagaaccaag ccattaattt aaatacgtta
      661 tgtaatccta aaaacttact accgtttgga gaagtaaagg caacagccag aagcatagga
      721 aaatggactt ggaaaaattt tagtcgttct gcattctcca gcgttcaagc tcaccgagga
      781 agaatgaatg gtggaaagaa gaaaaataac tataaaaaac aatcgaatat agaagaaatg
      841 atcaggtttt taggaggtga aaaatgagag tcagcagaaa tggattaaca caaaaacaac
      901 tggcagaaaa atttaacatc tcagtcacaa cagtcataaa atacacagcc atagaaaggg
      961 aagagtatga atcaaaagcc aaggaaagac gcctgaaagc ttacacaatg cgttctaacg
     1021 gcttttctct taaagagata gcattagagc ttggatgctc ttacaacgca gcagcggctc
     1081 tcgtgaagcg atataagcat atagatatgc tgaagaacca ttctgttgta taataaattt
     1141 tagaaaatat agaaactgtg gagggtttat gaatttagaa acactatacc atcatgattt
     1201 aaaagaaaaa aaagaaacag atttttcgtt ttctatcgaa gaaagaaaat gggcggctga
     1261 ggcagtagcc gattgttata aaaggattga aaatggaact gctaaaaaac taaaagcaag
     1321 ggattgatta aatgtattca gtaatcaccg caaataatac gctggatgaa ttcgtcgaag
     1381 aatatctttc tgatgacgat ttcaaccagt tgataagaaa acttgaaaac atgccacata
     1441 caaggccgta ctgggaaaac acacgctata aatacattat agcagcaaag atatattttc
     1501 tttatgaaat agatgactct aatatgtatg ttttcatact ggcagcaaag tttaataatt
     1561 caaggaaaac atttaatttt gccaaaaccc caacacaaat aaaaaccatg aaaaaatgga
     1621 ttaagaacaa aaagaaaatt tatcaataaa gaattttcta taattttttt tcgtaatgat
     1681 tcaacaagaa gttaaccatt gtttttatat ttttaatgtc atcgtcaaaa tctgagtctt
     1741 ttaatttcat ttttttgttt tcctttttca tgtctttttg tctcttctct tcttttttca
     1801 aatttttctc tgacttcatc ttttcttgta tggacaaaaa acggtctgac gactttttga
     1861 aaaaattgaa caaatccact aaaatgtctt cttgtaaatc caattctaaa ttcaactgtg
     1921 ttagcctctg ctctatttct aagctgtcga tttctgactg tttcagcaat tttattaatt
     1981 cgttgttgtt caaaatcaat tgcttcaatt tgtcttgttc tttcttctct tggttcaatt
     2041 ttatcatttc tattattttg ctcatttaat acctcactac cattcttatt attatcaaaa
     2101 tcgttaaaaa caaatttaga gaaactatcg agatcaaagc tgtctgtatt tttatgttga
     2161 ttgttctgat cttcatggtc atttgttttt ttagctcttg gctcaataat gctcgcattt
     2221 tttttcggtg gtctgttata tctttcagca attcttcctg atctgttttt aagtaattta
     2281 tcaaagatct ctctatgttc tgcgagtgct cttttagcaa attctggatc tcttgttctg
     2341 tcgttttttg gtggttctct atataggttg taatctctgc cctcttcata aatgtctcct
     2401 tttaacactg tgggtttttc gtcttctggt gaaaacttaa tagatatctg attttttcta
     2461 actctgttga tgtgaatatc ttttgatttt aaataatcga tcaactcatc tctgttgttt
     2521 atttcttgaa caataatttt tccgaggatc tcgttctgta tctgttcaag ttttgctcgt
     2581 ttttcttctg ggatgttggt cggaaccctg atcaggctgt tcttttcgtc tttgtccaac
     2641 actgacgaaa agccgtgttc aaagttgttc ttctcacaaa acgcattgaa aagatgatga
     2701 tctgtattgt ggtagtaagt tttgaaggct ctgccgtctt gtagagcgtt aaacgttgta
     2761 tagttaagtt ctaaccttcc cttgtccata tgctgaaccc acatgatagg agggcggttt
     2821 tcaggtggaa caccagaaaa aagcacctct tcaaaatctg cgatcatctt ctgttttaag
     2881 ttttcatctg tgtcgttttc ctcaaaagag agaacacccg taatatatgg gtgagcaaat
     2941 tttttagact ttatataatc attaaaattc tcaatgtctg ctcttgttgt tgttgtcata
     3001 acctgaacaa atttgcgatt ttcttctgtg tccgtaggtc taactcttag aatataatta
     3061 agagaatgct caacattctt ctgtcttttt ttacctccta tctgtttgtg tatcattggc
     3121 agatcctctt tttatttttt cgatgtcgtc aacaatttga tcaaacaaat atgcgaactg
     3181 ttcaccattg ccagacaaaa aataatcaat gttagtgttc atttttttag cgatttgatt
     3241 aatattgtta gcaacattag atagcaaaaa taattgttcc ttgtcgtcgt ttttatgtat
     3301 tctatttttt agttttgtag tgcttgtcaa aagtaattca cgaatggcag cagctcttgt
     3361 taaatctaat cttttagtta attcgtcaac ttctgacatt tcttgatctg aaagacaaag
     3421 agaaacaaaa ttttttctct ggtatttttc tttataatca cgcatattct gtccttttct
     3481 tttagctttt tgattttgac cttaaggggg attgaagggg gcgaagccac ctcaccagta
     3541 aacaatacga agtattgtgc taactggcta tctaatacaa ttagtaatac aaaaaaaata
     3601 aaagtcaatc acgagggata gtaattaggc aaagtcatta aaaccccgcc taattaaatt
     3661 taataagatc acttaattaa tttataccca tcttttaaac taaaccatgt atgcataaat
     3721 acaatatttc taccaagcaa tttttttata tcagccggtg ttaatggctt atcatacaaa
     3781 acaaaatcag aaagaacctg ataatatgtt ttatcaggaa caccatagtg atctttcttc
     3841 aaaacctcac cactggcaat accatgagca acaatcccct ctcctgacct atataaaaaa
     3901 actaaggaac ctttttcgat ataagtcatt ttctctttat aaccatcctc gaatgtcgca
     3961 gcaataccat tagaaaccat aaataagtca tcatctttat catggacaat attagtattc
     4021 acgacataac aattaggata gataccagca tgtaaaccat catcagattg gttagccata
     4081 tcttttaaac tggtatcatc aaaaatataa cgtttaagaa gaagataagt aagctcatta
     4141 cgactataac cagaattctg actggcaata gtaaacaacc gatcttcatc caaagtaagc
     4201 ctaagattca cggtagagga aatctgttca acagcatctt ttttgtctaa cgattttaga
     4261 aacgacatac aaaccccaag tcagagaaaa catcagtaca ctaaccagaa cattagcaca
     4321 gagtagtaac cagtacaatg aagtttctct gaatggaaga acccccaaga gagggctgtt
     4381 atctgatatg gttaaggcga ttacttgaca agataaaagg tttactctgt gaagaatcaa
     4441 aaaaccaccc taggagcggg ctgttatctg atatggttaa ggcggttact tgacaaagga
     4501 aaaaaaccag ttaaaacaga agaaaagaca gaacccctaa tgctacggtc gccaaactca
     4561 tcgcattagg ggttcaggga aaaggaactg ctt
//
