LOCUS       NZ_CP115725             5198 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Citrobacter braakii strain GW-Imi-1b1 plasmid unnamed10, complete
            sequence.
ACCESSION   NZ_CP115725
VERSION     NZ_CP115725.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32653119
KEYWORDS    RefSeq.
SOURCE      Citrobacter braakii
  ORGANISM  Citrobacter braakii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Schneider,D.
  TITLE     Complete genome of Citrobacter braakii isolate GW-Imi-1b1 originated
            from hospital wastewater in Greifswald
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Schneider,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JAN-2023) Institute of Microbiology and Genetics (AG
            Daniel), University of Goettingen, Grisebachstr. 8, Goettingen
            37077, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: 24-OCT-2022
            Assembly Method :: Flye v. v2.9.1-b1780; Unicycler v. v0.5.0
            Assembly Name                :: C_braakii_GW-Imi-1b1
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 3255.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/09/2024 02:15:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115725.1.
            Bacteria and source DNA available from Dr. Daniela Zuehlke,
            Department for Microbial Physiology and Molecular Biology, 17489
            Greifswald.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Citrobacter braakii"
                     /mol_type="genomic DNA"
                     /strain="GW-Imi-1b1"
                     /isolation_source="ENVO:00002018"
                     /db_xref="taxon:57706"
                     /plasmid="unnamed10"
                     /geo_loc_name="Germany: Greifswald"
                     /collection_date="2020-10-01"
     gene            join(4983..5198,1..681)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25290"
     CDS             join(4983..5198,1..681)
                     /locus_tag="PGH06_RS00005"
                     /old_locus_tag="PGH06_25290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_272487362.1"
                     /translation="MADPILPPQFTLKSVNITNAFENTPPPQDFVFPGLLAGSVGALVS
                     PGATGKSFLMLEAAMAVAGGTDLLGFEPSKSGGVLYFAAEDPTVELQRRVHAVGAHLGQ
                     EARASVAKNLYLVDAFGQQNNIMDNDFRDFLISRCLRPDGSQAVRLIVFDTVSRIHQLD
                     ENSNRDMIQLIGQLETIAARTGAAIIFLHHVSKSSASNGVADQQQAARGASALIDNARW
                     CGFVVKMTKEEAEQFNIDEDERRFYVRYGVSKQNYGSPLAEKWMFRHEGGVLKIHDISK
                     PMPKAMKTATNFNGGRW"
                     /name="helicase RepA family protein"
     gene            681..1409
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25295"
     CDS             681..1409
                     /locus_tag="PGH06_RS00010"
                     /old_locus_tag="PGH06_25295"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="WP_272487363.1"
                     /translation="MAHRKTRFPTTTVHQARLQLYQPTRRPKAMTRVIETSWGKAEVTG
                     KIGQGHADIIESLFYHAMDSKPTEIGGLLLLVDPYKLRKSIGGGKQYAGKQTWEMLQGL
                     RDIRIDLTAKGFRAIGGIVDYVEESDATLVAHNGLERHLWRIAINPAFVKIMAEDLPLS
                     YDPAPLSAISTGVSQAIARLVLTHKDQPNGGWLLDELIKAVGAGSDSELRNRRRDVHGD
                     IENLAKIGVLIEGNRVFKRG"
                     /name="ABC transporter ATPase"
     gene            1898..2302
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25300"
     CDS             1898..2302
                     /locus_tag="PGH06_RS00015"
                     /old_locus_tag="PGH06_25300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_272487364.1"
                     /translation="MADKETLDARAAFRLPADVLAEWHTKAKAGGYKNLSDFLRSAVDS
                     RQVTGLPTPAAKKRSTDNGCDPALIQQLAKIGNNLNQIARALNECRKAGDVVEVVEVLA
                     VLRSNEAAIAELFPKLPKPPAHNKGAADAH"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2292..4916
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25305"
     CDS             2292..4916
                     /locus_tag="PGH06_RS00020"
                     /old_locus_tag="PGH06_25305"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="WP_272487365.1"
                     /translation="MHIKFLAHGRGSASKAAAYVLASHDHKGIERAGVEVLRGDPQTFA
                     AIADSLEFKNKYTSGVIAWSLEDNPTPEEINAVLDDFERVAFAGLEPDSYHYCAVLHEE
                     PNGAKHIHILIPNVHLTTGKAFNPAPPNWEKTFDPLRDAWNNEKGWARPDDPTRARLVQ
                     PRHTALINAAAAKAGLAVEPDTKDFLTQYLTQRVLGGFIENRADVLESLAELGEITRAG
                     KDYISIKPEGFDKAIRLKGALFHEQFSSQSWIEVSREVEAGRGANRAGREQITAEHRSR
                     ASEARAALAAAIERREDYNGRRYKKARISPAIDAGLEAEPNSRPDRGDQGAIPDPVQID
                     GNENGTRPTGEDQQRTDEPENRDRQGDQQPDSDTRNNDPEPDTDDSKANQNAIALLAGR
                     FAGRGATDSGHELGANPQSDLVAADNHQGLREGRQLPSDAGRGVDELPNGRQLEKTVQE
                     GGLNDSIGNLANSAIERINRAIQSAIESIRKTIRGLAESIEQNQSAIDGNKSAIENHDR
                     ELSNLATPANTVIANRERIAQGVKIVRSNNLNEIERFKQEINLVEYAQVMGYELDKKAS
                     SKASAVMRRNGEKIIIATDKDGHGIYFNVGDNRDNGSIIDFIQNRNNMNLGEIRKVLRP
                     WIGVSNQPVLRATTPAPKPIATTRDRAAAVAAWEALRPYSGDYLQAERKLSAATVAAFG
                     DRIRQDARGNVCFRHIDLDGVTGYEIKNKGFTGFATGGEKAVFTCRINEDKPVSKVIVT
                     ESAIDAMSYHEMNGVQGEGVVYLSFGGGMSDRQKQVLRDYLTVKGLTDTAQVIVATDND
                     PAGHAFALEIHKIRPDAVRHEPDNAKDWNDMLKLDVLSRSQRVLQQRQAKPPESEDSSP
                     SPF"
                     /name="toprim domain-containing protein"
ORIGIN
        1 ccttccaagt ctggcggcgt actgtacttt gccgccgaag atccaaccgt cgagttacag
       61 cggcgagttc atgcggtagg cgcacatctg gggcaagagg caagggcgtc agtggctaaa
      121 aacctgtatc tggtggacgc tttcggccag cagaacaaca tcatggacaa cgatttccgc
      181 gactttctga ttagtcgttg ccttcgccct gatggttcgc aggccgtgcg gttgatcgtg
      241 ttcgataccg tgtcacgaat ccaccagctt gacgaaaaca gcaaccgcga catgatccag
      301 ctcattggcc agcttgaaac tatcgcggca cgaactgggg cggcaatcat tttcctgcac
      361 cacgtcagca aatcatcggc cagtaacggc gtggccgacc agcaacaggc ggcgcgtggg
      421 gcttcggcac tgatcgacaa tgcgcggtgg tgtggctttg tcgtcaagat gaccaaggaa
      481 gaagccgagc aattcaacat cgacgaggac gagcgccgtt tttatgtgcg ttatggcgtg
      541 tcaaaacaga actacggttc accactggct gaaaaatgga tgttccggca cgaaggcggc
      601 gttttgaaaa tccacgacat cagcaagccc atgcctaaag ccatgaaaac cgcgaccaac
      661 ttcaacgggg ggcgctggta atggctcata gaaaaacaag gtttccgaca accacggttc
      721 accaagcgag attgcagctt tatcaaccga cccgcagacc caaagcgatg actagggtaa
      781 tcgaaacatc atggggaaaa gccgaggtaa cagggaaaat tgggcaaggt cacgccgaca
      841 ttatcgagtc gcttttttat cacgcaatgg acagcaaacc cacagaaata ggcggtttgt
      901 tgctgcttgt tgacccttac aaattaagga aatcaatcgg cggcggcaag cagtatgcag
      961 gcaagcagac gtgggaaatg ttgcagggat tgcgcgacat tcgcatagac ctgaccgcta
     1021 aaggctttcg tgcaattggt ggcattgtcg attatgtcga agaatcagat gcaacgcttg
     1081 tggctcacaa cgggctagag cgtcacttgt ggcgaatagc tatcaatccg gcctttgtca
     1141 aaatcatggc tgaagacttg ccgctttcct acgatccagc tccgttgtct gcaattagta
     1201 cgggggtatc tcaagcgatt gctaggctag tcttgaccca caaagaccaa cccaatggcg
     1261 gctggcttct tgatgaacta atcaaggcgg tgggcgctgg gagtgatagc gagcttagaa
     1321 accgcaggcg tgatgttcat ggtgatattg aaaacctcgc caaaatcggg gttttaatcg
     1381 agggaaacag ggtcttcaag agggggtagc gtgtagcata ttcctgttcg tgtgtagcat
     1441 attcctgttc gtgtgtagca tattcctgtt cgtgtgtagc atattcctgt tccctttagg
     1501 tttctttagg agcttctagg ctcctaaagg aaagccgcct tgaagcggct tttttaacgt
     1561 caaaagcaaa gtcaaaaccg agtgcttcgc actctgggcg gtagggaagg gaaattggca
     1621 cggcgcgatt atttcctccc ctactgcccg caagacccct tggggggtcg cgcagcaggg
     1681 ggtaaagcat gccgcttgcg gctgctcatt gcttttgatt tggctcttga cttggccttt
     1741 ggtttgtggt ttagtgtttt aagccagccc acaccgtcga acaaaagagc cgcttcgcgt
     1801 ctctttttat tctcggtggg ctggtgaggg cgttccgccc ttcaatccct gtcgccgctt
     1861 cgcggctcag aaaaccaaca ccggaggcat cgaccaaatg gccgacaaag aaacactcga
     1921 cgcacgcgcc gcctttcggc ttcccgctga tgtgctggcc gagtggcaca ccaaagccaa
     1981 ggcaggcggc tacaagaatt tgtccgattt tctccggtcg gcggtggaca gtcggcaggt
     2041 tactggcctg ccaactccag ccgccaaaaa gcgcagtacc gataacggct gcgatccggc
     2101 attgatccag cagcttgcca aaatcggcaa caacttgaac cagatagcgc gagccttgaa
     2161 cgagtgccgg aaggctggcg acgtggtgga agtggtggaa gttttggccg tgttacgcag
     2221 taacgaagcg gcgattgccg agcttttccc gaaactgcca aagccaccag cacacaacaa
     2281 aggggcggca gatgcacatt aaattcctag cccacggcag aggcagcgca agcaaagccg
     2341 ccgcctatgt actggcaagc cacgatcaca aagggattga gagggcaggg gtggaagtgt
     2401 tgcgcggcga tccgcagacc ttcgcggcca ttgctgacag cttggaattt aaaaacaagt
     2461 acacgtcggg cgtcattgct tggagcttgg aagacaaccc aacgccggaa gaaattaacg
     2521 ccgtgctcga tgactttgag cgcgtggcct ttgctggact tgagccagac agttaccact
     2581 attgcgccgt acttcacgaa gaacccaacg gcgcgaagca cattcacatt ctgattccga
     2641 atgttcactt aaccactggc aaggctttca acccagcgcc accgaattgg gaaaagactt
     2701 tcgacccgct gcgtgatgct tggaataacg aaaagggatg ggcgcgaccc gacgacccga
     2761 cccgcgcgcg attggtgcag ccaaggcata cggcattgat taatgcagca gcggctaagg
     2821 ctggcttggc tgttgagcca gacacaaaag attttcttac ccaatacctg acccagcgcg
     2881 tacttggcgg atttatcgaa aaccgcgcag atgtgcttga gtcattggcc gagctgggcg
     2941 agataacccg cgcaggcaaa gactacatca gcataaagcc ggaaggattc gacaaagcaa
     3001 tcaggctcaa aggagcactt ttccatgaac aattcagcag ccaatcttgg atcgaagtta
     3061 gccgagaagt tgaagcagga cgcggagcta atcgagcagg ccgagagcaa atcacagcag
     3121 agcatcgaag ccgcgcaagt gaggctaggg cagcacttgc agcggctatt gagcgacgtg
     3181 aagattacaa cggacgacgc tacaagaaag ctcgaattag tccagcaatt gacgcaggac
     3241 ttgaagcaga gcctaacagc cgaccagatc gaggcgatca aggcgctatc cccgatcctg
     3301 tccaaattga cggaaacgaa aacggcactc gaccaactgg agaagatcaa cagcgcactg
     3361 acgagccaga aaaccgcgat agacaaggcg atcagcagcc agacagtgac actagaaaca
     3421 acgatccaga gccagacaca gacgattcaa aagctaatca gaatgccatt gctttacttg
     3481 ctgggcgctt cgctggccgt ggcgctactg attccggcca tgagctgggt gctaaccccc
     3541 aaagtgatct ggtcgcagcc gacaaccatc aaggactacg ggaaggcagg cagttaccaa
     3601 gtgatgctgg gcgaggggtg gacgaattgc ccaatggacg acaactcgaa aagaccgtgc
     3661 aggaaggtgg attaaatgac tcaattggaa accttgctaa ttcagcaatt gaaagaatta
     3721 accgcgcaat tcaatcagca atcgaatcaa tcagaaaaac aattcgagga cttgcggaat
     3781 caattgaaca gaaccaatca gcaattgacg gaaacaaatc agcgattgaa aaccatgacc
     3841 gagaattatc aaaccttgca acaccagcta acactgttat cgcaaacaga gaaagaattg
     3901 ctcagggagt taagattgtg agatcgaata acctcaatga aatcgagcgt ttcaagcaag
     3961 aaattaacct tgttgagtac gctcaggtaa tgggctacga gctagacaag aaggccagca
     4021 gcaaagccag tgcggtaatg cggcgtaatg gcgaaaagat tattatcgcc accgataaag
     4081 acggccacgg catttatttt aatgtcggtg ataatcgtga caatggctca attatcgact
     4141 ttatccaaaa ccgcaataat atgaaccttg gcgaaattcg caaggtattg cgcccttgga
     4201 ttggtgtttc taatcagcca gtattgcgag ccaccacgcc agcaccaaag ccaattgcaa
     4261 ccacccgcga cagggcggca gcggtggccg cttgggaagc attacgcccg tacagtggcg
     4321 actacctcca agcggaacgc aagctgtcag cggcaacggt ggcggcgttt ggcgatcgaa
     4381 tcaggcagga cgcaaggggt aacgtgtgct ttcggcacat tgatcttgat ggggtaacgg
     4441 gttacgaaat caaaaacaaa ggctttacgg gctttgccac tggtggcgaa aaggccgttt
     4501 tcacttgccg gatcaacgaa gataagcccg tttcaaaagt gattgtcacg gaaagcgcaa
     4561 tcgacgccat gagctaccac gaaatgaacg gggtgcaggg tgaaggcgtg gtttacttgt
     4621 cgtttggcgg tggcatgtca gaccgtcaga agcaggtttt gcgcgactat ctgaccgtca
     4681 agggcttgac cgatacggct caagtcattg tcgccacgga caacgacccc gcaggccatg
     4741 cgtttgcctt ggaaatccac aaaatccggc ctgatgctgt gcgccatgag ccggacaacg
     4801 cgaaggactg gaacgacatg ctgaaattgg acgttttaag ccgttctcag cgcgtcttgc
     4861 agcaaagaca ggccaaacca ccagaaagcg aagacagttc gcctagcccg ttttaaacgg
     4921 ccttggcggg tgcttgagac acccgccaga cctaaccact cacgaaaaca ggactttcat
     4981 ttatggctga cccaatttta ccacctcaat tcaccctcaa aagcgtcaac atcacgaacg
     5041 catttgaaaa cacgccaccg ccacaagatt ttgtcttccc tggactgctt gccgggtcgg
     5101 ttggtgcctt ggtttcacct ggcgcgactg gcaaatcatt cttgatgctt gaagctgcaa
     5161 tggcggtcgc aggcggtacg gatttgctgg gcttcgag
//
