LOCUS       NZ_CP115705             8905 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Klebsiella pneumoniae strain 2020CK-00201 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP115705
VERSION     NZ_CP115705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907136
            Assembly: GCF_027886565.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00201
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8905)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 03:49:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,455
            CDSs (total)                      :: 5,332
            Genes (coding)                    :: 5,225
            CDSs (with protein)               :: 5,225
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 48 of 107
            Pseudo Genes (incomplete)         :: 60 of 107
            Pseudo Genes (internal stop)      :: 15 of 107
            Pseudo Genes (multiple problems)  :: 15 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00201"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2019-07-23"
     gene            complement(260..682)
                     /locus_tag="PF326_RS27160"
                     /old_locus_tag="PF326_27160"
     CDS             complement(260..682)
                     /locus_tag="PF326_RS27160"
                     /old_locus_tag="PF326_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_022644822.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(682..873)
                     /locus_tag="PF326_RS27165"
                     /old_locus_tag="PF326_27165"
     CDS             complement(682..873)
                     /locus_tag="PF326_RS27165"
                     /old_locus_tag="PF326_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1289..1432
                     /locus_tag="PF326_RS27300"
     CDS             1289..1432
                     /locus_tag="PF326_RS27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671098.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="hypothetical protein"
     gene            1722..2099
                     /locus_tag="PF326_RS27170"
                     /old_locus_tag="PF326_27170"
     CDS             1722..2099
                     /locus_tag="PF326_RS27170"
                     /old_locus_tag="PF326_27170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            2156..2461
                     /locus_tag="PF326_RS27175"
                     /old_locus_tag="PF326_27175"
     CDS             2156..2461
                     /locus_tag="PF326_RS27175"
                     /old_locus_tag="PF326_27175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058876158.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGDLSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            2599..2841
                     /locus_tag="PF326_RS27180"
                     /old_locus_tag="PF326_27180"
     CDS             2599..2841
                     /locus_tag="PF326_RS27180"
                     /old_locus_tag="PF326_27180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(3079..>3249)
                     /locus_tag="PF326_RS27185"
                     /old_locus_tag="PF326_27185"
                     /pseudo=""
     CDS             complement(3079..>3249)
                     /locus_tag="PF326_RS27185"
                     /old_locus_tag="PF326_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(3304..4008)
                     /locus_tag="PF326_RS27190"
                     /old_locus_tag="PF326_27190"
     CDS             complement(3304..4008)
                     /locus_tag="PF326_RS27190"
                     /old_locus_tag="PF326_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<4074..4289)
                     /locus_tag="PF326_RS27195"
                     /old_locus_tag="PF326_27195"
                     /pseudo=""
     CDS             complement(<4074..4289)
                     /locus_tag="PF326_RS27195"
                     /old_locus_tag="PF326_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(4359..4913)
                     /gene="aac(6')-Ib"
                     /locus_tag="PF326_RS27200"
                     /old_locus_tag="PF326_27200"
     ARG             complement(4363..4913)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5147..5704)
                     /locus_tag="PF326_RS27205"
                     /old_locus_tag="PF326_27205"
     CDS             complement(5147..5704)
                     /locus_tag="PF326_RS27205"
                     /old_locus_tag="PF326_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            5866..8871
                     /locus_tag="PF326_RS27210"
                     /old_locus_tag="PF326_27210"
     CDS             5866..8871
                     /locus_tag="PF326_RS27210"
                     /old_locus_tag="PF326_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             complement(4021..4277)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(4360..4964)
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          1032..1397
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 gattcacata tgaatcgtgg gcaattcgtc agcgattcgc catagagtcg tccacgactt
       61 ggatctgcac tttgtaacac atcaccgcac atttattttt cattaaccca ttgttgtaaa
      121 taatgaatct gcaaaatttt aattttgcgt ggggtgtggg tatttttagt ggtgaggagc
      181 gttcagagat ctggcgattt ttgggaaggt tcagaaactc tccgggatgg ccggagagga
      241 aaactcaagc atggcaggat caggattttt tccagaattt ccaccacggg gaatgggatg
      301 gtgaagaagg ttcgtgcttg tgctccagaa gctgcatagc ctgacgaagg gaatcaatat
      361 gctcatctcg agccttaaca gcccctttaa gaccatcgtt ttcagcttca agtaaagcga
      421 ttctatgttg taaatcatgt aaaccaacgc tgttaatacc tgtagattct gcgtcacttt
      481 ttacatcata ttttacagta tcagttacac catcaagaga accaaacacc ctaataagct
      541 cggatacatc aataacttta atatccttcc cgtgctttac aacagaggta taacttaact
      601 tcccgctgtt aatcatcttg taaatagtgg gacgactaac cttaacaagc cgtgcagctt
      661 cactaataga cactctggcc atcagttacc atcctgtaat gaatccagtc tcgaggagag
      721 tgtacggaaa agatgctctg catcatcgtg gagtttctca caaaggtcgg cctcagcatc
      781 aagatcaagc tcgttaagct tctccagcag gagcagagac tgattctgga tgaatttagc
      841 catgttcagc gccgttttct gttgtttcgt cataaagggc ctcagagcgt cagtgctcag
      901 tgtaaaacct gtctcttttc cgcttcctcg ctcactcagt cgctacgctc cggtcgttcg
      961 gctgcggcga gcggcaggag catgcataga aaagctcgag aaaaccgagc ggtcaaagga
     1021 ggaaaaaaag gaaaggccac ggcgttgccg tttttccata ggctccgccc ccctgacaag
     1081 catcgcagaa acggacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     1141 caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     1201 ggtgtcactc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg
     1261 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg
     1321 cgccttatcc ggtaactatc gtcttgagtc caatccggaa agacacgact tatcgccact
     1381 ggcagcagcc actggtaacg gattcgcaga acaaagaagt gttgaagtgt gaggtgaagt
     1441 acagctacac tgtaataact aatttggttt ctgtgctctg cgaaagccag ttacctcggt
     1501 tcaaagagtt gctgctctaa gaaccttcga aaaaaccgcc ttgctgggcg gttttttcgt
     1561 ttccagggca agagattacg acgcaacaga aaggatctca agaggatcct attccatccc
     1621 tctccccccc aaagggaaac cagcaccact ccacgacacc agttctttga agaaaattat
     1681 tgaacccctt gattttaata ataaaaaaaa cagatataat catgatccac aatcacccaa
     1741 cttgggtaat aaaatcaatg atttataggt taggtatttt atcaatatcg ctgcttacag
     1801 ttggttgtgt ttctaccagc aacgttcttg agttaggtag ggatacatat acggtttcag
     1861 caactgctga tggttttaga gatgcggcat cagcacgtca aagtgccttt aaaacaggtt
     1921 ctgagaaatg taatagcctc ggtaaaaaat tcctgctcac taacgaaaac gtggctaaca
     1981 caagaatggg gatagatacc acagtaacac tctacttccg ttgtcttgac gaaagagaca
     2041 gcgagtatgt acgaccaaat ctacagaaag cacctgatct acgcatcgag agtaaataat
     2101 tatgaatttc cttatgatca tgatcctatg gtggatatcg tcattcatca cacacatgat
     2161 gaaagttgga aatgtcagag agtacggtcc cactaaaagc catttactgt tgatggttac
     2221 tacgcttcct gctggcgttg gtgtttttat ctatccgata gtagaggtga tatggggaga
     2281 tctgtcgtta ctgaaagttg ctggttatac ctttttatcc ctcattgttg gcggacttct
     2341 tgccaatatc attgttcgcg gattatctgc gacaacattc ggcattttgc tcgtaaatcc
     2401 attagcaatg atatgtagca ttgccggact gatagtcctt gctatccaac atttaccata
     2461 acatccgtta tcagcaccgc ctgagaggcc ctcagggcgt ttctgacggg aaaccggtaa
     2521 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     2581 gcatgagtat gcgcgcaagt gcatagcgcg gaaattcaca ccattcagcc aggggaagtc
     2641 ctgtttccgg cgttcttctc ctccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     2701 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag caccttcagg aatcgggttc
     2761 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     2821 gcgattcgaa tcgtgaattg aatcaagagt caccatgagc aaaaaacacc caaagtgatg
     2881 aattgaatca agagtcgatt catcataaca atatgataat aaagcgtgat tggggtctga
     2941 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3001 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3061 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3121 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3181 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3241 agatttatca gggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag
     3301 gccttacatt tcaaaaactc tgcttaccag gcgcatttcg cccaggggat caccataata
     3361 aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc tcaatacctt tgatggtggc
     3421 gtaagccgtc ttcatggatt taaatcccag cgtggcgccg attatccgtt tcagtttgcc
     3481 atgatcgcat tcaatcacgt tgttccggta cttaatctgt cggtgttcaa cgtcagacgg
     3541 gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca taggcgggcg ctttatccgt
     3601 gttgatgaat cgcgggatct gccacttctt cacgttgttg aggattttac ccagaaaccg
     3661 gtatgcagct ttgctgttac gacgggagga gagataaaaa tcgacagtgc ggccccggct
     3721 gtcgacggcc cggtacagat acgcccagcg gccattgacc ttcacgtagg tttcatccat
     3781 gtgccacggg caaagatcgg aagggttacg ccagtaccag cgcagccgtt tttccatttc
     3841 aggcgcataa cgctgaaccc agcggtaaat cgtggagtga tcgacattca ctccgcgttc
     3901 agccagcatc tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     3961 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     4021 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgtttccaaa
     4081 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     4141 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     4201 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     4261 gatacttcgg cgatcaccgc ttccctcatg atgtttaacg tttgacatga ggggcggcca
     4321 agggcgccag cccttggacg tccccctcga tggaagggtt aggcaacact gcgtgttcgc
     4381 tcgaatgcct ggcgtgtttg aaccatgtac acggctggac catctggggt ggttacggta
     4441 ccttgcctct caaaccccgc tttctcgtag catcggatcg ctcgcaagtt gctcggcgac
     4501 gggtccgttt ggatcttggt gacctcggga tcattgaaca gcaactcaac cagagctcga
     4561 accagcttgg ttcccaagcc tttgcccagt tgtgatgcat tcgccagtaa ctggtctatt
     4621 ccgcgtactc ctggatcggt ttcttcttcc caccatccgt ccccgcttcc aagagcaacg
     4681 tacgactggg catacccaat cggctctcca ttcagcattg caatgtatgg agtgacggac
     4741 tcttgcgcta aaacgcttgg caagtactgt tcctgtacgt cagcaagtgt cgggcgtgct
     4801 tcttctccgc cccaccactc gacgatatga gatcgattta gccactcata gagcatcgca
     4861 aggtcatgct cagtcatgag gcgcagtgtg acggaatcgt tgctgttggt cacgatgctg
     4921 tactttgtga tgcctaactt tgtttggaaa tgttgaatac tcatactctt cctttttcaa
     4981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5041 tagaaaaata aacaaatagg ggttccgcgc acttttcccc gaaaagtgcc acctgacgtc
     5101 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5161 cgtcttcaag aattttataa accgtggagc gggcaatact gagctgatga gcaatttccg
     5221 ttgcaccagt gcccttctga tgaagcgtca gcacgacgtt cctgtccacg gtacgcctgc
     5281 ggccaaattt gattcctttc agctttgctt cctgtcggcc ctcattcgtg cgctctagga
     5341 tcctccggcg ttcagcctgt gccacagccg acaggatggt gaccaccatt tgccccatat
     5401 caccgtcggt actgatcccg tcgtcaataa accgaaccgc tacaccctga gcatcaaact
     5461 cttttatcag ttggatcatg tcggcggtgt cgcggccaag acggtcgagc ttcttcacca
     5521 gaatgacatc accttcctcc accttcatcc tcagcaaatc cagcccttcc cgatctgttg
     5581 aactgccgga tgccttgtcg gtaaagatgc ggttagcttt tacccctgca tctttgagcg
     5641 ctctgatctg aatatcgagg gactgctggc tggttgagac ccgcgcataa ccaaaaattc
     5701 gcataaaaat gtaccttaaa tcgaatatca gacacgatgt gtctattatg ccaaaatgac
     5761 gatttaatgg acactcaaac gaagccgttt tactatgtct gataatttat aatatttcga
     5821 acggttgcag ttgtgttaaa aaagccgtca ggcagggagg ccgatatgcc cgttgatttt
     5881 ttgaccactg agcaggttga gagttatggc aggttcactg gcgaacccga tgaacttcag
     5941 ctggcgcgtt attttcatct tgatgaagcg gataaagaat ttatcgggaa aagccggggt
     6001 gatcacaatc gacttggtat tgccctgcaa atcgggtgtg tgcgttttct gggcactttt
     6061 cttactgaca tgaatcatat tccttccggc gtccggcatt ttaccgccag acagctcggg
     6121 attcgtgata tcaccgttct tgcagaatac ggtcagaggg aaaatacccg ccgtgagcat
     6181 gcagcgctga tacgtcagca ctatcagtat cgtgaatttg cctggccctg gacatttcgc
     6241 cttacccgtc ttttatatac ccggagctgg ataagcaacg aacgtcctgg cctgcttttc
     6301 gacctggcga cagggtggct tatgcaacat cgtattattc tccccggagc caccacgctg
     6361 acccggttga tttcagaggt aagggaaaag gcgacgttgc gcctgtggaa caaactggca
     6421 ctgataccgt cagccgaaca gcgttcacag ctggagatgc tgctggggcc aactgattgc
     6481 agccgcctgt ctttactgga atcactgaaa aaaggccctg tgaccatcag tggtccggcg
     6541 tttaatgaag caattgaacg ctggaaaact ctgaacgatt ttggcctgca tgctgaaaac
     6601 ctgagtacac tcccggctgt gcgcctgaaa aatctcgcac gttatgctgg tatgacttcg
     6661 gtgttcaata ttgccaggat gtcaccgcag aaaaggatgg cggttctggt tgcctttgtc
     6721 cttgcatggg aaacgctggc gctggatgat gcactggacg ttctggacgc catgctggcc
     6781 gttatcatcc gtgacgccag aaagattggg cagaaaaaac ggctccgctc gctgaaggat
     6841 ctggataaat ctgcattggc gctcgccagc gcatgttcgt acttgctgaa agaagaaaca
     6901 ccggacgaat cgattcgtgc tgaggtgttc agctacatcc ctaggcaaaa gctggctgaa
     6961 atcatcacgc ttgtccgtga aattgcccgg ccctcagacg ataattttca tgacgaaatg
     7021 gtggagcagt acgggcgcgt tcgtcgtttc ctgccccatc tgctgaatac cgttaaattt
     7081 tcatccgcac ctgccggggt taccactctg aatgcctgtg actacctcag ccgggagttc
     7141 agctcacggc ggcagttttt tgacgacgca ccaacggaaa tcatcagtca gtcatggaaa
     7201 cggctggtga ttaacaagga aaaacatatc acccgaaggg gatacacgct ctgctttctc
     7261 agtaaactgc aggatagtct gagacggagg gatgtctacg ttaccggcag taaccggtgg
     7321 ggagatcctc gtgcaagatt actacagggt gctgactggc aggcaaatcg gattaaggtt
     7381 tatcgttctt tggggcaccc gacagacccg caggaagcaa taaaatctct gggccatcag
     7441 cttgatagtc gttacagaca ggttgctgca cgtcttggcg aaaatgaggc tgtcgaactc
     7501 gatgtttctg gcccgaagcc ccggttgaca atttctcccc tcgccagtct tgatgagccg
     7561 gacagtctga aacgactgag caaaatgatc agtgatctgc tccctccggt ggatttaacg
     7621 gagttgctgc tcgaaattaa cgcccatacc ggatttgctg atgagttttt ccatgccagt
     7681 gaagccagtg ccagagttga tgatctgccc gtcagcatca gcgccgtgct gatggctgaa
     7741 gcctgcaata tcggtctgga accactgatc agatcaaatg ttcctgcact gacccgacac
     7801 cggctgaact ggacaaaagc gaactatctg cgggctgaaa ctatcaccag cgctaatgcc
     7861 agactggttg attttcaggc aacgctgcca ctggcacaga tatggggtgg aggagaagtg
     7921 gcatctgcag atggaatgcg ctttgttacg ccagtcagaa caatcaatgc cggaccgaac
     7981 cgcaaatact ttggtaataa cagagggatc acctggtaca actttgtgtc cgatcagtat
     8041 tccggctttc atggcatcgt tataccgggg acgctgaggg actctatctt tgtgctggaa
     8101 ggccttctgg aacaggagac cgggctgaat ccaaccgaaa ttatgaccga tacggcaggt
     8161 gccagcgatc ttgtctttgg ccttttctgg ctgctgggat accagttttc tccacgcctg
     8221 gctgatgccg gtgcttcggt tttctggcga atggaccatg atgccgacta tggcgtgctg
     8281 aatgatattg ccagagggca atcagatccc cgaaaaatag tccttcagtg ggacgaaatg
     8341 atccggaccg caggctccct gaagctgggc aaagtacagg cctcagtgct ggtccgttca
     8401 ttgctgaaaa gtgaacgtcc ctccggactg actcaggcaa tcattgaagt ggggcgcatc
     8461 aacaaaacgc tgtatctgct taattatatt gatgatgaag attaccgccg gcgcattctg
     8521 acccagctta atcggggaga aagccgtcat gcagttgcca gagccatctg tcacggtcaa
     8581 aaaggtgaga taagaaaacg atataccgac ggtcaggaag atcagttggg agctctgggg
     8641 ctggtcacta acgccgtcgt gttatggaac actatttata tgcaggcagc tctggatcat
     8701 ctccgggcgc agggtgaaac actgaatgat gaagatatcg cacgcctctc cccgctttgc
     8761 cacggacata tcaatatgct cggccattat tccttcacgc tggcagaact ggtgaccaaa
     8821 ggtcatctga gaccattaaa agaggcgtca gaggcagaaa acgttgctta acgtgagttt
     8881 tcgttccact gagcgtcaga cccct
//
