LOCUS       NZ_CP115699             3815 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00202 plasmid unnamed3,
            complete sequence.
ACCESSION   NZ_CP115699
VERSION     NZ_CP115699.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907137
            Assembly: GCF_027886545.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00202
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:02:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,770
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,583
            CDSs (with protein)               :: 4,583
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 28 of 70
            Pseudo Genes (incomplete)         :: 39 of 70
            Pseudo Genes (internal stop)      :: 13 of 70
            Pseudo Genes (multiple problems)  :: 10 of 70
            Pseudo Genes (short protein)      :: 1 of 70
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115699.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00202"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA"
                     /collection_date="2019-06-27"
     gene            complement(1037..1378)
                     /locus_tag="PF328_RS23790"
                     /old_locus_tag="PF328_23790"
     CDS             complement(1037..1378)
                     /locus_tag="PF328_RS23790"
                     /old_locus_tag="PF328_23790"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184457.1"
                     /translation="MAKPPSATPPDTPSSCDVSALPGIIQNFGLINFNVSCNNGELTVT
                     GTDQNGKHIRVNSYQGNGFTEGTMSTFQPGGKEARRLEAVRLRESGLTQQEIANRLGVS
                     QKTISNDLK"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1983..2198)
                     /locus_tag="PF328_RS23795"
                     /old_locus_tag="PF328_23795"
     CDS             complement(1983..2198)
                     /locus_tag="PF328_RS23795"
                     /old_locus_tag="PF328_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2205..2696)
                     /locus_tag="PF328_RS23800"
                     /old_locus_tag="PF328_23800"
     CDS             complement(2205..2696)
                     /locus_tag="PF328_RS23800"
                     /old_locus_tag="PF328_23800"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012564908.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAVFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2731..3435)
                     /locus_tag="PF328_RS23870"
                     /pseudo=""
     CDS             complement(<2731..3435)
                     /locus_tag="PF328_RS23870"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3374..3697)
                     /locus_tag="PF328_RS23810"
                     /old_locus_tag="PF328_23810"
     CDS             complement(3374..3697)
                     /locus_tag="PF328_RS23810"
                     /old_locus_tag="PF328_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2..169)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.491"
                     /coverage="97.0"
                     /evalue="1.23e-64"
     Typing          136..644
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1892..3384)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggggcgcagc cctgaaccag tcacgtagcg ctagcggagt gtatactggc ttactatgtt
       61 ggcatggatg cggaaatcag tgaagtgctt catgtggcag gagaaaaaag gtggcaccgg
      121 cgcgtcaaca ggagatgcaa tacaggagat attccgcttc ctcgctcact gactcgctgc
      181 gctcggtcgt tcggctgcgg cgagcggtac cggcttacga atggggcgga aatttcctgg
      241 tagatgccag gaaggaactt aacagggaaa cgataaggcc gcggcaaagc cgtttttcca
      301 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
      361 cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctcg tgcgctctcc
      421 tgttcctgcc tttcggttta ccggtgtcac tccgctgtta tggccgcgtt tatctcattc
      481 cacgcctgac actcggttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
      541 cccccgttca gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      601 aaagacacga aaaaacgcca ctggcagcag ccactggtaa caggattagc agagcgagat
      661 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actggaagga
      721 cagtatttgg tatctgcgct ctgctgaagc cagttaccag gttaagcagt tccccaactg
      781 acttaacctt cgaaaaaccg cctccccagg cggttttttc gttttcagag caagagatta
      841 cgatgcaatg gaaaggatct caagaagatc attattgatg ttcgacatat atggggatct
      901 gcaccatggc ttaaaataaa catctgaaat gtatctttag gtgtttcacc actgcgggac
      961 gagcaactac gccggattta tcatggcatt tcagtagcga tgaaaagccc gttaagacgg
     1021 gcttaaagca agtgactcat tttaaatcat tgcttatagt tttttgagaa acgcctaacc
     1081 ggtttgctat ttcttgctgc gtaagcccac tttctcttaa acgaacagcc tcaagtcttc
     1141 tggcctcttt acctccgggc tgaaacgttg acattgtacc ttcggtaaag ccgttccctt
     1201 ggtagctgtt aactcttatg tgtttcccat tttgatccgt acccgttacc gttagctcac
     1261 cgttattgca ggatacgtta aagttaatta agccaaaatt ttggataatg cctgggagag
     1321 cactaacatc acatgaagat ggtgtatccg gtggtgtagc tgatggaggt tttgccattg
     1381 tgcgttcctt gatttttacg atgcatccat actagtaaat actagaaaac tagtaatatc
     1441 aataatccat atccctttag gccatcaccg aagcccctgc ttatagtcac ttctccccct
     1501 acatttacca taacatccgt tgcccgcacc gctgaaacgg ggttggctgc gtttccggtg
     1561 ccagacgcga gaaagtggcg acagacataa cgaaaaaaca ccagaaacgc agccgctgaa
     1621 tattacactg gtgcatagtc atgcagccag atgcatggat gttaaagtca cactaaaatc
     1681 cgcgccaatg ccttaaaaac cgacaaatgc ttctccagcc agtcccacag ctccgccgcc
     1741 agatacaggc agttaagcaa tgattcaata agatttaaca gcttcatttc ccgctcctga
     1801 ataagtcgtg acctttttga tcacgaacct atttagcggg agaaataaga gcccctcttc
     1861 gtgcgggttg tcagctgcct ggatggttta aagtgtcggc ccgcggtagg ttttctgcgg
     1921 ctggcgctcc agctccagtg ttttctgata ctcattctcc ctctgccgga actcggtggc
     1981 attcagttcc cggacaagcg gcgcagctgc tcgcttaaac tcagcacctg cgtgctcaag
     2041 tccgacacgc gctcgttcag caccgcgttc tcccgttgca taagaccaca catgctccgc
     2101 cattccgcga aggcgctctc ccattcgtcc agcctttgcg agtagtcctg ctgtagctgc
     2161 tctaatgcgc tcagcaactg cttttccagc tctgtcattt tccgctactc ctgtttcagg
     2221 cggatccatg tcgtcccgtc gtaggggatg gcttcggttt gcatccccgg cggcaggata
     2281 agaaacctcc ggccgtcgct gctctcctga tagcgcactc cccacgtccg ggcggtcatt
     2341 ttcgccaggg tatcttcctg ctgacgaaga tgcgtgtaat tgtcggttat ctgctgcccc
     2401 tgccaccaca gaatgctccc gctcgtgccg atcagcagcg ctgacaccat caggatggtg
     2461 agccatgtcc gcccggcagt ccgcagtacg ctccgccggt ttttctccat cgcctcgctc
     2521 atactctgct cgtgggcgag gatggcgtcg ctgattctcc tcgcgcttgc gctcagttcc
     2581 gctctgacag accgctcgtg ctcgctgaat acggctttca gcatctcgcc ggtactctgc
     2641 tgctgcgctt tcgattgctg ctctaagtcc ttcgccagcg ttaaaagact gctcatagat
     2701 ggctcccttc agtcggatgt ttcgcccccc gtccgggtcg gcaatgctga tactgctttt
     2761 cgtggtgcgc accacctcaa aacctgcacc ttcaagagtg tcagtgacat cctgacggct
     2821 tttcagctcc ccggacgaag caagggtcag cagtccccgg gtaatggctt ctgcggcctc
     2881 ctgcttcgtt ttcggcagcc cggacggcgt gaccagcgcc cggcggtttt ccggcgcgtt
     2941 cgggtcgtgc agccccagtc tgccgttcac gatagtctgc caggcatcga tgcgcggccg
     3001 gtcggcgcgg tcgtagtaag gctggagccg cctgccggtc agcagctcgg tattggggat
     3061 cagaaagttc agctccagac gccccttgtc cgcgtgctcc acccacagaa tgctgtactg
     3121 atctttatcg agtccgggca tcagaacccg ctcgaagctg gccatcagtt tttcccgctg
     3181 gccgggaggc agatcggcct ccgcaaacga cagaacgccg gaggtgtatt tcttaacgta
     3241 aggcgacgcg tcaatcagct cccggacttc ttccggcttc ccctgcagga cggtggcgcc
     3301 ttcacgctgc cggtctttac ccagcagata gtccaccggc ccggcgccac cgccgcggcc
     3361 gcgcggatga aatttaacga tcatcgtccg ttcctttctc aagaacggcg tgccgcagcc
     3421 gctcaaggcc ggcatcgatg gccatcagcg ccgccacaat ctgtaccctg tcatggccgg
     3481 tgccgccgcc ggcgttaaca cggcgggcta tctggttcag gttattgccc atgccggcaa
     3541 gctggcgcag cagcgccggc gagagtgagg ggagctttcc ggaacgtgcc ggcttttcgt
     3601 ccaggcagat ctgccgcatc cacgccgcca gctgcctgcc gtcacagcgc tcgagcagcc
     3661 gccggtgctc gtcctccgtc acccacatgg tgagcatctt gctccgtttc tctgacaaac
     3721 cggcctcctg ataacgccgg gacgggcggg aaagcctttc ccggtcggcg cggggttgga
     3781 caagccgcag gcgcgtcagg cttttggcgg gtttc
//
