LOCUS       NZ_CP115695             2495 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00204 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP115695
VERSION     NZ_CP115695.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907139
            Assembly: GCF_027886525.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00204
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,061
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 28 of 128
            Pseudo Genes (incomplete)         :: 100 of 128
            Pseudo Genes (internal stop)      :: 12 of 128
            Pseudo Genes (multiple problems)  :: 13 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115695.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00204"
                     /isolation_source="Tracheal Aspirate/Wash"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="1929-09-24"
     gene            complement(join(2369..2495,1..59))
                     /locus_tag="PF325_RS25250"
                     /old_locus_tag="PF325_25250"
     CDS             complement(join(2369..2495,1..59))
                     /locus_tag="PF325_RS25250"
                     /old_locus_tag="PF325_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(425..652)
                     /locus_tag="PF325_RS25255"
                     /old_locus_tag="PF325_25255"
     CDS             complement(425..652)
                     /locus_tag="PF325_RS25255"
                     /old_locus_tag="PF325_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1860..2078
                     /locus_tag="PF325_RS25260"
                     /old_locus_tag="PF325_25260"
     CDS             1860..2078
                     /locus_tag="PF325_RS25260"
                     /old_locus_tag="PF325_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          2235..2322
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1068..1596)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 agtgtctgtg cgcggataaa tttggccata ttgagggcgg cggattcctg tctggtcact
       61 ggtgattttc cccgtctggt gatgattttt gcgtgaaaag agcatggcgt cattctggtg
      121 catagtcatg catgccgtta aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt
      181 tgttgtggtt tttgtcatgg tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg
      241 gcaacggatg ttatggtaaa ttcttggggg cttgattata aaataccgca cttcctctta
      301 tgtcttgtgc tttttagaca accctgaaag cttttagctt tggtgatgca atttgctggg
      361 attttggcgg agaaccacag gtaaagaaaa aggccacatt agcggccttt ttcggagaag
      421 atgctcagtg cggaatttcg gagacttctt taacgtcggt tttattgatc tgctgtttga
      481 caccattcgc gtctgtatag ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc
      541 tgacaatagt gcgaccgtca ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga
      601 gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc aaagccagct
      661 ccttttatcc atatcagttt tggtcactaa ccatctaact ataggtcatt ttttatgtga
      721 aaagaggctt gatggtgggc tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt
      781 ggtgtcaggc ggggaggagg cttatatgag atgtaatccg gccgatacat ataccaagat
      841 gatttaagta ttcctctttg tgaggttgga tgtctaattt ataaaggatc ttcttgagat
      901 cctttttttc cgcgcgtaat ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt
      961 ttttcgaagg ttaggtaatc ctggcagatc ccctaaccgt ggtaacagtc ttgtgcgaga
     1021 catgtcacca aatttgtcct ttcagtgtag cctcactaag gccgccactt caagaactct
     1081 tgagacatct ctcgcacatc ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt
     1141 ctttccgggt tggactcaag ttgatagtta ccggataagg cgcagcggtc ggactgaacg
     1201 gggggttcgt gcatacagtc catcctggag cgaactgcct tcccggaact gagtgtcagg
     1261 cgtggaatga gaaaccgcgg ccataacagc ggagtgacac cggtaaaccg aaaggcagga
     1321 atgcggggga gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agccgcatcg
     1381 ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc
     1441 tatggaaaaa cggctttggc gcggccttat gctttcttcg ttaagtatct tcctggcatc
     1501 ccccaggaaa tttctgatcc atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga
     1561 gcggagcgag tcagtgagcg aggaagcgga atatattctg tatcacattt tctcctgacg
     1621 cgttttcttt cactttctgc gcctgtctta tgtggcatta atgctatgtg ttactgccat
     1681 gctacatctt aagccagtat acactccgct agtgctccgt gactggtccg gcgctgcgcc
     1741 cggaacccgc ctgtaccggt tcagcagccg ttccggcctg actgcaattt tttttttttc
     1801 atccctgccc gctaccctgt aaacctttct tctgcgttgc cgttaacagt aggtaagcca
     1861 tgtttggttt ttcttccgga gccctgatga ctcttagtga tgatgagcga cacctgcttg
     1921 tgtccgttgt ttcggtctgg ctacggcgtg ccggagggga tgccggagcc atgatgctgg
     1981 atgcataccg gcagattttg tctgaaaccg aaccggccgt aaggactgtc atgctcgaat
     2041 ttcttgaatc cgttcgcatt cattacattt cgtcctgagc attttctgta tttctgtcca
     2101 tacttaaacg aggcggccgt cacggacggt ggtcgtcgtt ccatgatgct gttatggacg
     2161 agtgagagta atctaatcta acttgattga gttccgggcg tgccgcccgg ttttttttgc
     2221 tgtttcccgg tgtccgttcc gggacaggat gtgcatttgt aataccgcgc acgcgccggc
     2281 atcacaaata ccctggtcag ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc
     2341 actgaattgc gcacccgttc accgattttc acgcattttc ctcgcctatc tgtgcgttca
     2401 gcatggcgta aagcgcttcg gcctgatcgt gcaggtgctc acagcagccg gcagcctcat
     2461 ccagatccat ctgctcgagc cgctcaagga ggaga
//
