LOCUS       NZ_CP115694             4938 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00204 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP115694
VERSION     NZ_CP115694.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907139
            Assembly: GCF_027886525.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00204
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,061
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 28 of 128
            Pseudo Genes (incomplete)         :: 100 of 128
            Pseudo Genes (internal stop)      :: 12 of 128
            Pseudo Genes (multiple problems)  :: 13 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115694.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00204"
                     /isolation_source="Tracheal Aspirate/Wash"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="1929-09-24"
     gene            305..628
                     /locus_tag="PF325_RS25225"
                     /old_locus_tag="PF325_25225"
     CDS             305..628
                     /locus_tag="PF325_RS25225"
                     /old_locus_tag="PF325_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            837..>1271
                     /locus_tag="PF325_RS25230"
                     /old_locus_tag="PF325_25230"
                     /pseudo=""
     CDS             837..>1271
                     /locus_tag="PF325_RS25230"
                     /old_locus_tag="PF325_25230"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1306..1797
                     /locus_tag="PF325_RS25235"
                     /old_locus_tag="PF325_25235"
     CDS             1306..1797
                     /locus_tag="PF325_RS25235"
                     /old_locus_tag="PF325_25235"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_016355729.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESNDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1804..2019
                     /locus_tag="PF325_RS25240"
                     /old_locus_tag="PF325_25240"
     CDS             1804..2019
                     /locus_tag="PF325_RS25240"
                     /old_locus_tag="PF325_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2948..4234
                     /locus_tag="PF325_RS25245"
                     /old_locus_tag="PF325_25245"
     CDS             2948..4234
                     /locus_tag="PF325_RS25245"
                     /old_locus_tag="PF325_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     Typing          18..190
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="9.14e-86"
     Typing          complement(4485..4938)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.824"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          306..622
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="2.38e-59"
     Typing          619..2120
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.692"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcgca tattctgctg
       61 acgctccgct gctgcatttt tctcctgcca catgaagcac ttcactgata ttcgcatccg
      121 tgccaacata gtaagccagt atacactccg ctagcgctac gtgactggtt cagggctgcg
      181 ccccgaaacc cgctaacagc cactgacgcg cctgcggctt gtccaacccc gcgccgaccg
      241 ggaaaggttt tcccgcccgt cccggggtta tcaggagact gttttgtcag agaaacggaa
      301 caagatgctc accatgtggg tgacggagga tgagcacagg cggctacttg agcgctgcga
      361 cggcaggcag ctggcggcgt ggatgcggca gacctgcctg gacgaaaagc cggctcgtac
      421 cgcaaaactt ccttcgatct cgccggcgct gctgcgccag cttgccggta tgggcaacaa
      481 cctgaaccag atagcccgcc gtgttaacgc cggcgtcggt accggtcatg accgggtgca
      541 gattgtggcg gcgctgatgg ccatcgatgc cgggcttgag cggctgcggc acgccgttct
      601 tgagaaagga acggacgatg atcgttaaat ttcatccacg cggtcgcggc ggtggcgccg
      661 ggccggtgga ctatctgctg ggtaaagacc ggcagcgtga aggcgccagc gtcctgcagg
      721 ggaagccgga agaagtccgg gagctgatcg acgcgtcgcc ttacgtgaag aaatacacct
      781 ccggcgtact gtcgtttgcg gaggccgatc tgcctcccgg ccagcgggaa aaactgatgg
      841 ccagcttcga gcgggttctg atgcccggac ttgataaaga tcagtacagc attctgtggg
      901 tggagcacga ggacaagggg cgtctggagc tgaactttct gatccccaat accgagctgc
      961 tgaccggcag gcggctccag ccgtactacg accgcgccga ccggccgcgt atcgatgcct
     1021 ggcagaccat cgtgaacggc agactggggc tgcacgatcc gaacgcgccg gaaaaccgcc
     1081 gggcgctggt cacgccggcc ggactgccga aaacgaagca ggaagccgca gaagccatca
     1141 cccgtggact gctgagctta gcctcgtccg gggagctgaa aagccgtcag gacgtcactg
     1201 aggctcttga aggtgcaggt tttgaggtgg tgcgtacaac gaaaagcagt atcagcattg
     1261 ccgaccctga cggggggcga aacatccgac tgaagggagc catctatgag cagtctttta
     1321 acgctggcga aggacttaga gcagcaatcg aaagcgcagc agaagagtac cggcgagatg
     1381 ctgaaagccg cattcagcga gcacgagcag tctgtcagag cggaactgaa cgcaagcgcg
     1441 aggagaatca gcgacgccat cagcgcccac gagcagagta tgagcgaggc gatggagaaa
     1501 aaccggcgga gcgtgttgct gacggccggg aggacatggc tcaccatcct gatggtgtca
     1561 gcgctgctga tcgccacgag cgggagcatt ctgtggtggc agggacggca gataaccgac
     1621 aattacacac atcttcgtca gcaggaagat accctggcga aaatgaccgc ccggacgtgg
     1681 ggagtacgct atcaggagag caacgacggc cggaggttcc ttatcctgcc gccggggatg
     1741 caagccgaag ccatccccta cgacgggacg acctggatcc gcctgaaaca ggagtagcgg
     1801 aaaatgacag agctggaaaa gcagttgctg agcgcattag agcagctaca gcaggactac
     1861 tcgcaaaggc tggacgaatg ggagagcgcc ttcgcggaat ggcggagcat gtgtggactt
     1921 atgcaacggg agaacgcggt gctgaacgag cgcgtgtcgg acttgagcac gcaggtgctg
     1981 agtttaagcg agcagctgcg ccgcttgtcc gggaactgaa tgccatcgag gagaaggctg
     2041 cactggagta tcagcgtcag aacgagctgg agctgacgct ccgacagaag cagaagagtt
     2101 tcgatggtcc gaagatgagg tgacagcccg cacgaagaag ggctcatcgc gttcctgcta
     2161 aataggctga ctaccggaat ggtagtgact tatcccggag gaagtgatga aaattctcaa
     2221 cgttattgaa gccctgctga actgttttta cctggtgatg gagctgtgtg agttgccgga
     2281 gaggcatttg ccggttttta aggcgttcgc gctgatttta gtgtgacttt acgctgcatg
     2341 catctggctg catgactatg catcagtgta atatccagcg gctgcgtttt tggtgttttt
     2401 tcgtcatgtc tgtcgccact ttctcgcgtc tggcaccgga aacgcagcca accccgtttc
     2461 tgcggtgcgg gcaaggggtg ttatgttaaa agccgaggtc tggcggatac ggaaaaaatt
     2521 atgccggccg ggcttatgcc cgcggcaatc cggccgatac ttaaattatc cttcacaaag
     2581 gatgtgctgc gatccaggca gttgagtgtt ttccggctcc cggatgtgag ccattttttt
     2641 gccttctgat gcctgttaat gcatgcggag ctgcgctggt tcttccaaga ttgatagacg
     2701 ccagcttctc tgctggagcc ggcggatttg ccgtaaatat gaacattttc ttattgttca
     2761 gcccgctaaa gatggcgggt tttttacgcg tgaaatccgg agccggacat catgccggca
     2821 tggctgaaga gcgggctttt tcccgccctg tcgcagtaat atataacgct acataaggag
     2881 tagccgataa ccatggcagt acttacatta tccgggcaat ctgattcata tgttttcact
     2941 taaaaatatg ttctccctga acctccagcg atcagccatt tcgtatgacg caatttgtca
     3001 gaataagcct gtcattgagt tcagtccgga gggggtgata aataaagcca gcccgctttt
     3061 tctctccact atggggtaca gggctgatga aataatcggt caccaccatc gtatattctg
     3121 cccgccctcg ctggtaagtt caccggaata cagccagttc tggcagcgcc ttgccagggg
     3181 agaaagcttt agtggcaagt acctgcggct ggcaaaggga aatcgttccg tatggcttga
     3241 agccagttat atccccgttt ctgacaggcg cggccgtgtt atcagagtca ttaaaattgc
     3301 tgccgatatt tctgagcgcg tgcattctgc tcttgagcag gaagcagtcg taaatgccat
     3361 aaaccgttct atggccatca tcaccttcaa tcctgaaggg atcgtgcttg aagcaaatga
     3421 aaatttcgtc aatgccactg gctataagcg ggatgaaatc ataggtaagc atcatcgtct
     3481 gttctgtgcc gagacgcttt acaaaagtga tgaataccga catttctggg agagtctgaa
     3541 tcaaggtgag tttttttctg gtctgtttcc acgccttaac cgtcagggag accctctgtg
     3601 gttccgtgca acttataacc ctgtctttaa cagtgatggg cagctttata aaattgtgaa
     3661 attcgctaca gatgttacgg ctgatgtcct gcgaaaccag agagagcaag aggctgctgt
     3721 gcatgcatgg gatatggccg tgcagacccg agaaagtgcg cagaacggtg ccaatgttat
     3781 tgaaaatagc atccttatga ttgacagaat tgcgcagggg atgggggctg tctccaccga
     3841 tatctcacgg ctcaacaatc agtctgaaag tattgacgat atggtggaaa ccatccggaa
     3901 gtttgccatg cagacaagac ttattgcact gaatgccgcg attgaagcag caagggctgg
     3961 cgcatccgga agaagttttg cggttgttgc cgcagaagtg cgtaatcttg cggcgagcgt
     4021 cagtagcgcg actgaagaaa ttgagcaagt cgtggccagc aatagtcaac tggccaagga
     4081 tgttctctgt ggcattgaaa acagcctgat gaacactcgg gaaggggtta cactcatgcg
     4141 cgaagcgggt gaagttatga ccagcattca gaggaatgct gcaggggttg agactgcggt
     4201 taaggatgtc gccatttcag ttaaagccgg ataggccatg gtgcatatcc ccatatctgt
     4261 cgaacatcac agatgatctt cttgagatcc tttccgttgc gtcgtaatct cttgctctga
     4321 aaacgaaaaa accacccggc aaggtggctt tttcgaaggt tcgcaagagt tgacgctctt
     4381 ttgaaccgcg gtaactggct tggaggagca cagtcaccaa aactgtcctt tcagtgtagc
     4441 cttatcgcac ccgaaacttc aagactctcc tcctctaaac ctgttaccag tggctgctgc
     4501 cagtggcgct ttgtcgtgtt tttccggatt ggactcaagt tgatagttac cggataaggc
     4561 gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgcctt
     4621 cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc ggaatgacac
     4681 cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccaccaggg ggaaacgcct
     4741 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag atttcgtgat
     4801 gcttgtcagg ggggcggagc ctatggaaaa acggcttcgc cgcggcctta tcgcttccct
     4861 gttaagcttc tttctggcat ctcccaggaa atttccgccc cgcctgtaag ccggtaccgc
     4921 tcgccgcagc cgaacgac
//
