LOCUS       NZ_CP115693             5042 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00204 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP115693
VERSION     NZ_CP115693.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907139
            Assembly: GCF_027886525.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     2020CK-00204
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:00:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,061
            CDSs (total)                      :: 4,946
            Genes (coding)                    :: 4,818
            CDSs (with protein)               :: 4,818
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 28 of 128
            Pseudo Genes (incomplete)         :: 100 of 128
            Pseudo Genes (internal stop)      :: 12 of 128
            Pseudo Genes (multiple problems)  :: 13 of 128
            Pseudo Genes (short protein)      :: 1 of 128
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115693.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00204"
                     /isolation_source="Tracheal Aspirate/Wash"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="1929-09-24"
     gene            69..212
                     /locus_tag="PF325_RS25315"
     CDS             69..212
                     /locus_tag="PF325_RS25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072052415.1"
                     /translation="MHEPPVQSDCCTLPGNYHLESNPERHEKTPLAAATGNGFAEQRSV
                     EV"
                     /name="replication initiation protein"
     gene            145..474
                     /locus_tag="PF325_RS25190"
                     /old_locus_tag="PF325_25190"
     CDS             145..474
                     /locus_tag="PF325_RS25190"
                     /old_locus_tag="PF325_25190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_180259235.1"
                     /translation="MKKHHWQQPLVMGSQNKEVLKCEANYSYTVRTNSVSVLCESQLPR
                     FRNLPVNEPSKNHLAGWFFRLQSRRLRLIAKDLKKILYGLLFDPLSYVTLGAMKGGTLL
                     VHDAF"
                     /name="hypothetical protein"
     gene            complement(550..1101)
                     /locus_tag="PF325_RS25195"
                     /old_locus_tag="PF325_25195"
     CDS             complement(550..1101)
                     /locus_tag="PF325_RS25195"
                     /old_locus_tag="PF325_25195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_131657980.1"
                     /translation="MAIYIRKVTKSKWDWKNPVSHTVNASTLGTDGVTNCCKTSGNTLS
                     IWKIDSETLASDNDKKIVATLATNGGSLSPIDCVIFSDDDIKSCGLAVDQKDGESLISD
                     VKSMHYNIVGLDMNSVSALGLMINRKVSGVRNGNEIIQKTQLKKIDLGSIKSCINQFIP
                     KTSEAGNALILKSGFSKVYE"
                     /name="hypothetical protein"
     gene            complement(1109..1750)
                     /locus_tag="PF325_RS25200"
                     /old_locus_tag="PF325_25200"
     CDS             complement(1109..1750)
                     /locus_tag="PF325_RS25200"
                     /old_locus_tag="PF325_25200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045891080.1"
                     /translation="MAHIYTNKNWVQDNIAKVDSYYDQVVLNYLNKVYIKDYESNKSST
                     GVNTYVELTDNNSRGMNLKSEDSYELDKIMSFVYTMLVSEDFGYGDTPELKYVVENIFR
                     RSKSEAQMAFINMATRRVYTSDSDIIEKFFALLMSLEEHDIDNIALPIIQMFLSHKDVS
                     AAEGALSLLDKYGKDRDALDVAKQIRDFEYDFLNDYKEKVICNLKKYAEE"
                     /name="hypothetical protein"
     gene            complement(1759..2427)
                     /locus_tag="PF325_RS25205"
                     /old_locus_tag="PF325_25205"
     CDS             complement(1759..2427)
                     /locus_tag="PF325_RS25205"
                     /old_locus_tag="PF325_25205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045891079.1"
                     /translation="MFEFQFSYFVRERFVDSSPESIFPLITAFENNFLPSYADEVSPNG
                     VRKVIRMVGINDFDNIAVLFSSEAININMLYRDVRPSKEQVIAKLDFIVSALSKIKSNT
                     KAWRIASVVTSIEKATQSYNSKMYSKYLSDGASDVCFEWNVRQAKKTQINSEILNIVTS
                     LSRGPVMNSANPTESFDAVVVQLDVNTAPENASERFEVSNGLAAQLVDMAVENIDIVLA
                     "
                     /name="hypothetical protein"
     gene            complement(3204..3422)
                     /locus_tag="PF325_RS25210"
                     /old_locus_tag="PF325_25210"
     CDS             complement(3204..3422)
                     /locus_tag="PF325_RS25210"
                     /old_locus_tag="PF325_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223866370.1"
                     /translation="MFGFSSGDLMTLSDDERHLLVSVVSGCLRRAGGDAGGMMLDAYRQ
                     ILSETEPTARTVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(4110..4496)
                     /locus_tag="PF325_RS25215"
                     /old_locus_tag="PF325_25215"
     CDS             complement(4110..4496)
                     /locus_tag="PF325_RS25215"
                     /old_locus_tag="PF325_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045891078.1"
                     /translation="MAKISISEAARLTGKSRTTLHRLIKTGELSTCHGARNARMVDVSE
                     LIRVFGPLEHRPSEQGAAQVSEHSDTGISAQSEQVIAQLRQEVEHLKTLVSAKDSHIDS
                     LKQAMLLIEHRHTGDSPWWKFWKR"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4496..4687)
                     /locus_tag="PF325_RS25220"
                     /old_locus_tag="PF325_25220"
     CDS             complement(4496..4687)
                     /locus_tag="PF325_RS25220"
                     /old_locus_tag="PF325_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045891077.1"
                     /translation="MTKQEKTALNMAKFIQGQSLLLLEKLNELNLDMEADMCEKLHEDA
                     EMLCRTLYGRLNALQDAG"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3527..3607)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.771"
                     /coverage="93.0"
                     /evalue="5.42e-25"
     Typing          2..358
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="98.0"
                     /evalue="8.61e-115"
     Typing          4870..5042
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.678"
                     /coverage="86.0"
                     /evalue="5.31e-67"
ORIGIN
        1 gccgcgttta tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
       61 tggactgtat gcacgaaccc cccgttcagt ccgactgctg caccttaccc ggtaactatc
      121 atcttgagtc caacccggaa aggcatgaaa aaacaccatt ggcagcagcc actggtaatg
      181 ggttcgcaga acaaagaagt gttgaagtgt gaggcaaatt acagctacac tgttagaacg
      241 aattcggttt ctgtgctctg cgaaagccag ttaccacggt tcagaaactt gccggttaat
      301 gaaccctcga aaaaccacct tgccgggtgg ttttttcgtt tacagagcag gagattacga
      361 ctgattgcca aggatctcaa gaagatcctt tatggattat tgtttgatcc actgtcgtac
      421 gtaacattag gcgctatgaa aggtggaacg ctgttagttc atgatgcttt ttaaagaccg
      481 ccgtgtaagc cggtctctta ttgggtattg gtctactgcg tcggtagtca aaaatttaat
      541 ttcaaaaaat cattcataca ctttgctgaa accgctcttt aatattaatg cgtttcctgc
      601 ctcactagtt tttggtataa attgatttat acaggacttt atacttccaa ggtctatttt
      661 ttttaactgg gttttctgaa tgatttcatt tccatttctt acaccagaaa ctttcctatt
      721 aatcattaat cctagtgcgc taacactatt catatccaaa ccgactatat tgtaatgcat
      781 tgatttaaca tcagatatca aagattctcc gtctttctga tcaacagcta aaccacatga
      841 tttaatatca tcatcactaa aaataacaca atcaatcgga gataagcttc caccgttcgt
      901 ggctaaggta gcgacaattt ttttatcatt gtcactagct aaagtttcac tatcgatttt
      961 ccatatggac aaggtattac ctgaagtctt acagcaattg gtaaccccat cggtacctaa
     1021 ggtactggca ttcaccgtat gactaaccgg gtttttccaa tcccatttag attttgtcac
     1081 cttcctaata taaatagcca ttgtaaactc actcttcagc atatttcttc aggttgcaaa
     1141 taactttttc tttataatca ttgagaaaat cgtactcaaa atccctgatt tgttttgcta
     1201 catctaatgc atctctatcc ttaccatatt tatctaaaag tgatagagct ccctcagctg
     1261 cactgacatc cttatgagaa agaaacatct gaataatagg aagcgcaata ttatcaatat
     1321 cgtgttcttc caaagacatt agaagtgcaa aaaacttctc aatgatatca ctatcagaag
     1381 tataaacccg tctagttgcc atgtttataa atgccatttg agcttcagat ttacttcgcc
     1441 taaatatatt ttctacaaca tattttaatt ctggagtatc tccataccca aaatcctcag
     1501 acactaacat agtataaaca aaggacatta ttttatctaa ctcataggag tcctctgact
     1561 ttaagttcat accacgacta ttattgtcag ttaattcaac atatgtatta accccggtcg
     1621 atgatttatt actctcataa tcttttatat aaactttatt taagtaattc aaaaccacct
     1681 ggtcatagta tgaatctact ttggcgatat tgtcttgaac ccagttttta tttgtataga
     1741 tatgagccat aagccacctc aagccaatac tatatcaata ttttcaacag ccatatcaac
     1801 gagttgcgca gccaagccat ttgaaacctc gaacctttcc gatgcgtttt ctggagcggt
     1861 gttgacatcc agctgaacta caacagcatc aaaactttcg gttgggttag ctgagttcat
     1921 aaccgggcct cggctcaaag aagtaacaat gttcaatatt tcagaattta tctgtgtttt
     1981 ttttgcttga cgaacgttcc attcaaaaca aacatcggaa gcaccatcac taagatattt
     2041 actgtacatt ttagaattgt atgattgagt cgctttctca atagaagtaa caacagaagc
     2101 aatacgccac gcttttgtat tcgatttgat ttttgataaa gcacttacta taaaatccag
     2161 tttagcgatg acttgttctt tactaggcct tacatcccta tacaacatat ttatattaat
     2221 agcttcagac gaaaataaaa cagcgatatt atcaaagtca ttaataccta ccattcgaat
     2281 tacttttcga acgccattag gcgatacctc atcggcataa cttgggagaa agttattttc
     2341 aaaagctgta attaatggaa agatactttc aggggaacta tcaacgaatc tttctctgac
     2401 aaaataacta aattgaaact caaacataaa aactcctttt tgtttttgaa catacacgag
     2461 caatcataac ccgcaaacgc ccgtcattta gctaggcgtt caatgatggt tattgtatat
     2521 cacaatttag ttattttatt gactcgacgc tcttatttca tcatcaaagc ataagaatag
     2581 gcataattac ttagagtatg agtattctac gcaaacgaat cagagatcag atgcaatgat
     2641 ttaccataac gcctcattac gcgcaccgct cagatgcgct cagcgcgttt ctgacgggaa
     2701 acgggtaaaa caggtaacaa acccccggca aacccgccag aaacgcaggg gatgaattct
     2761 ggcacagtgc atggctatgc agccaggtga atggctctga ttccggcgcc ggatccaccc
     2821 cgtaacctct ctttttcaca ctttccgggc ctgccggtgt gcatttctgg caggtgattt
     2881 tgcgcatgcg caaaatcacg ctgagaaaaa ggaagtggtg aacgggcgcg caatttattg
     2941 cgtgaccgca aagcgcttgc cgggtgtcgg ggcggcgagc cctgacagga acacttgcca
     3001 tgccgcgtgt gtgcggtatt acaaatgcac attctgtccc ggagtggaca ccaaaagaca
     3061 gcccgaaaaa aaccgggcga gccgcccgga aggtaaatca agttagatta gattactctc
     3121 tcatccataa gagcatccca caacgacgac catcatccct gacggccgcc tcgtttaagt
     3181 atggacagaa atgcagaaaa tgctcaggac gaaacgtaat gaatccggac ggattcaaga
     3241 aattcaagca tgactgtcct tgctgtcggc tctgtttcag acaaaatctg tcggtatgca
     3301 tccagcatca tgcctcccgc atcccccccg gcacgccgga ggcaaccgga aacgacggaa
     3361 acaagcaggt gtcgctcatc atcactaaga gtcatcaggt ccccagaaga aaaaccaaac
     3421 atggctatat cctgttaacg gcaacgcaga agaaaggttt acagggtagc gggcaggaat
     3481 gaaaaaaaaa cacagacagt ccggaacctc agctgaaccg gtacaggcgg gtttcggggc
     3541 gcagccctga ccagagtatt tgtgatgcca tcgcgtacgc ggtattacaa atgcacatcc
     3601 tgtcccgtgg cgctgacacc ctcaaaaaaa ctgaagccgt gaaaattccc tgaaaatgcc
     3661 ggcaagccac agtgccggat ccggcaccac actggatata aatcagtaac acaatcctgc
     3721 cacctggata aatatcatgt gggcggatcc ggcactgctc gcgggtatac aggtgtgtgg
     3781 atccggcacc agtgccggat tatgtgacat gtaagccact gaaaaatagc gatagccaca
     3841 atccggcgat atgacagtgc cggtaccgac acctggaggt gtgctaaatg tgtgatactt
     3901 ttcggggtaa atgtaaatcg cctgaaatat ttaagattat attactgata tagaagacaa
     3961 aatctgcaag acgaagtctt gcgtggggtg tgatgttttg accctgaaga cacagagcgg
     4021 cgatcgggct gtgtggacag cctgaaaaaa caggtttggg ctaagtcatt caataaatca
     4081 gacaagcctc gccggctact gaggcgttct cagcgtttcc agaacttcca ccagggtgag
     4141 tccccggtat gcctgtgctc gatcagcagc atcgcttgtt tgaggctatc gatgtgtgaa
     4201 tccttggcgg aaactaacgt tttcaggtgt tccacttcct gccgcagctg tgcaataacc
     4261 tgttcactct gcgcggaaat acctgtgtca gagtgttcag aaacctgtgc agctccctgt
     4321 tcagacgggc gatgctcaag agggccaaaa acacggataa gttccgaaac atcgaccatt
     4381 ctggcgttcc ttgcaccatg acaagtggac agttcacctg ttttaatcaa cctgtgcaac
     4441 gttgtccgac tcttcccggt cagccgtgcg gcctcactga tcgatatctt ggccattaac
     4501 ctgcatcctg tagagcattt aaccgcccat agagagtcct gcagagcatc tctgcatcct
     4561 catggagttt ttcacacatg tccgcttcca tgtcgaggtt aagctcattc agcttctcca
     4621 acaacaaaag ggactgccct tggatgaact tcgccatatt aagcgcggtt ttctcttgtt
     4681 tcgtcattag caggcctcag tacgtacgtg cttaatgtgg ggacttgctc cattctgctt
     4741 cctcgcccgt tcgctcgcaa ggctcggtca cgggtctgcg gcgagcagta cagactgtca
     4801 aggtcaccca caaattcagt ggataacctg cagggaaagg tgtgagggaa aagtaaggct
     4861 gcggcgaagc cgtttttcca taggcttcgc ccccctgata agcatcacag aatcggacgc
     4921 tcaaatcagt ggtggcgaaa cccgacagga ctataaagat cccaggcgtt tccccctggt
     4981 agctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta
     5041 tg
//
