LOCUS       NZ_CP115686             3549 bp    DNA     circular CON 09-MAR-2024
DEFINITION  Klebsiella oxytoca strain 2020CK-00200 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP115686
VERSION     NZ_CP115686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907135
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-AUG-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 02:13:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00200"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:571"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA"
                     /collection_date="2020-04-21"
     gene            complement(601..1023)
                     /locus_tag="PF584_RS00005"
                     /old_locus_tag="PF584_34385"
     CDS             complement(601..1023)
                     /locus_tag="PF584_RS00005"
                     /old_locus_tag="PF584_34385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1023..1214)
                     /locus_tag="PF584_RS00010"
                     /old_locus_tag="PF584_34390"
     CDS             complement(1023..1214)
                     /locus_tag="PF584_RS00010"
                     /old_locus_tag="PF584_34390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1619..1780
                     /locus_tag="PF584_RS00035"
     CDS             1619..1780
                     /locus_tag="PF584_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     gene            complement(1938..2093)
                     /locus_tag="PF584_RS00015"
                     /old_locus_tag="PF584_34395"
     CDS             complement(1938..2093)
                     /locus_tag="PF584_RS00015"
                     /old_locus_tag="PF584_34395"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(2201..2458)
                     /locus_tag="PF584_RS00020"
                     /old_locus_tag="PF584_34400"
     CDS             complement(2201..2458)
                     /locus_tag="PF584_RS00020"
                     /old_locus_tag="PF584_34400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(2460..>3062)
                     /locus_tag="PF584_RS00025"
                     /old_locus_tag="PF584_34405"
                     /pseudo=""
     CDS             complement(2460..>3062)
                     /locus_tag="PF584_RS00025"
                     /old_locus_tag="PF584_34405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            <3061..3402
                     /locus_tag="PF584_RS00030"
                     /old_locus_tag="PF584_34410"
                     /pseudo=""
     CDS             <3061..3402
                     /locus_tag="PF584_RS00030"
                     /old_locus_tag="PF584_34410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          1263..1729
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 gtatgcgcgc aagtgcatag cgcggaaatt cacgccattc agccagggta agtcctgttt
       61 ccggcgttct tctgatccga aaacggtgaa cggggacgca atttattgcg tggccgtgag
      121 gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt caggaatcgg gttcgtcgac
      181 ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc accggcgatt
      241 cgaatcgtga attgaatcaa gagtcaccat gaacaaaaaa cgcccaaatt gatgaattga
      301 atcaagagtc gattcatcat aacaacatga taataaagcg tgattcacat atgaatcgtg
      361 ggcaattcgt aaacgattcg ccatagagtc gtccacgact tggatctgca ctttgtaaca
      421 catcactgca cattaatttt tcattaacca attgttttaa ataaggaatc tgcaaaattt
      481 taattttgcg tagtgtgtgg gtatttttag tcgtgaggag cgttcagaga tctggcgatt
      541 tttgggaagg ttcaggaact ccctgggatg gccggagagg aaaactcaag catggcagga
      601 tcaggatttt ttccagaatt tccaccacgg ggaatggggt ggtgaagaag gctcgcgctt
      661 gtgctccaga agctgcatag cctgacgaag ggaatcaata tgctcatctc gagccttaac
      721 agccccttta agaccatcgt tttcagcttc aagtagagcg attctatgtt gtaaatcatg
      781 taaaccaacg ctgttaatac ttgtagattc tgcgtcactt tttacatcat attttacagt
      841 atcgatgaca ccatcaagag aaccaaaaac tctaataagc tctgaagtat caataacttt
      901 aatagccttc ccgtgcttta caacagaggt gtaacttaac ttcccactgt taatcatctt
      961 gtaaattgtg ggacggctaa ccttaacaag ccgtgccgct tcactaatag acactctggc
     1021 catcaattac catcctgtaa ttcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     1081 gcatcatcgt ggagtttctc acaaaggtcg gcctcaacat cgagatcaag ctcgttaagc
     1141 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc
     1201 tgttgtttcg tcataaaggg cctcagagcg tcagtgctca atgtcagaca gatctctttt
     1261 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtcgca
     1321 acgcgcacat aacgacatag agcagacagc tcggagaggg agaaggcagc acagcggccg
     1381 tttttccata ggctccgccc ccctgacaag catcacagaa tttgacgctc aaatcagtgg
     1441 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctgatgg ctccctcgtg
     1501 cgctctcctg ttcctgccat tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     1561 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     1621 gcacgaaccc cccgttcagt ccgactgctg cgtcttatcc ggtaactatc atcttgagtc
     1681 caacccggaa agacacgaca aagcgccact ggcagcagcc actggtaacg gatttagagg
     1741 agttggtctt gaagtcatgc gcctgttaag gctaaactga aagaacaagt tttggtgact
     1801 gcgctcctct aagccagtta ccttggttca atcacctgca agtgtttgaa ccttcggaaa
     1861 aaccgccctg caaggcggtt ttttcgttta cagagcaaga gattacgacg atccaaatgg
     1921 gatctcaaga agatccttta cggattgccg ttcgtaccgc tatcaagcgt gactccggtc
     1981 accgtcgatg aaggtgaaac agccccacct ccggtgtctc gaacattatt gacctgacat
     2041 gctgccagaa ccacagaaac ggtaacaacc cctaggaaaa gtaatttttt catatatcac
     2101 cttttatttt tattattcta ggcaaatgga tatctagtaa ttattcttca aatccagagt
     2161 tacaaatgag cgggctcata acttgaaccc gcaaattcaa tcacgctttt ttaaaacctg
     2221 gcttaccgtt agcagctcgc cattccttta cggttttaac aattcctttc gccgaagaat
     2281 cttcgcctac cttaggcttg aagatgaggt tccaaccagc aggatgctct gttagttggg
     2341 cgaacgaata aatagcatca tcgttctctt tgtcagttgg aaaatcagca gcgattattt
     2401 tactaacaaa agcagtaaat tcttcttccg taaaatctga aattttatta ccagccattt
     2461 tattttcctt tatgtgtctc aatatgattt ttaggtgtta gcgccttaat gttatcgaca
     2521 tccataacag cccctccttg agatatttct ttttcgtggt gtaattcaat tttaacacgt
     2581 ttccctactg attctaaaaa gtccgcagta ggtgcccttc cagctttcat ctgattaata
     2641 tcagcttcac taaattgctt gctcagtgca gaatcgtcag caaccgcttt ccagaacgcc
     2701 cgacggaaag aatcaaaact accgaaagtc ttcccgcgta atttatccgc aatctgggaa
     2761 ggaacaggag caccatcccc ggtactggcc ccccccatcc agttattgcc aacgttctgc
     2821 cctttgccgc tcactgtacc gggcatgtta cgagggctac ggtacatcac atacagcggt
     2881 tttaggccgg attccggagg aaacaccaga ataatatcgt caaaatcgag gtcatccgct
     2941 accggcgtca cggttttatc tgtagcctgt ggcgccgaaa tctgatcacc ggtatgcaga
     3001 atacgctccg gtagtggtac cggcccgctt agcgtcagtg gtccgtttgc accaggtgcg
     3061 tcaagtgcac tagatttcac caggatggcg cttaatcagg aaaaatacct cgatgacttt
     3121 acatggaaag aactgaacga cgttatcaac ttcatacgca aagcagaagg tagtgcatct
     3181 gaccacagct atgcactcca gttactggag attaatttca aggcaaaccc actggatctc
     3241 atctatcacc ctgactgctg gttcaatgat gaagcattgt ttcatgcccg gctgactacc
     3301 gaagaaattg gtggctactt aatgaaaaaa tcaggtcgat ggctcaacga tgcaccggat
     3361 atccaactgg tgtatgccat cccccaggat gtttatgatt aaccaagtca ccatttacca
     3421 taatccctta attgtacgca ccgctaaaac ggcctcagga cgtttctgac gggaaaccgg
     3481 taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg
     3541 catgcatga
//
