LOCUS       NZ_CP115678             3815 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00203 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP115678
VERSION     NZ_CP115678.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907138
            Assembly: GCF_027886505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-AUG-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,975
            CDSs (total)                      :: 4,857
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 14 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115678.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00203"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2019-03-02"
     gene            590..1081
                     /locus_tag="PF324_RS24830"
                     /old_locus_tag="PF324_24830"
     CDS             590..1081
                     /locus_tag="PF324_RS24830"
                     /old_locus_tag="PF324_24830"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012564908.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAVFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1088..1303
                     /locus_tag="PF324_RS24835"
                     /old_locus_tag="PF324_24835"
     CDS             1088..1303
                     /locus_tag="PF324_RS24835"
                     /old_locus_tag="PF324_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1908..2249
                     /locus_tag="PF324_RS24840"
                     /old_locus_tag="PF324_24840"
     CDS             1908..2249
                     /locus_tag="PF324_RS24840"
                     /old_locus_tag="PF324_24840"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184457.1"
                     /translation="MAKPPSATPPDTPSSCDVSALPGIIQNFGLINFNVSCNNGELTVT
                     GTDQNGKHIRVNSYQGNGFTEGTMSTFQPGGKEARRLEAVRLRESGLTQQEIANRLGVS
                     QKTISNDLK"
                     /name="helix-turn-helix domain-containing protein"
     gene            3404..3727
                     /locus_tag="PF324_RS24845"
                     /old_locus_tag="PF324_24845"
     CDS             3404..3727
                     /locus_tag="PF324_RS24845"
                     /old_locus_tag="PF324_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000957084.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3118..3291
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          complement(2643..3151)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2..1395
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.36"
                     /coverage="93.0"
                     /evalue="0.0"
     Typing          3405..3724
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.458"
                     /coverage="100.0"
                     /evalue="1.38e-61"
ORIGIN
        1 caggggaagc cggaagaagt ccgggagctg attgacgcgt cgccttacgt taagaaatac
       61 acctccggcg ttctgtcgtt tgcggaggcc gatctgcctc ccggccagcg ggaaaaactg
      121 atggccagct tcgagcgggt tctgatgccc ggactcgata aagatcagta cagcattctg
      181 tgggtggagc acgcggacaa ggggcgtctg gagctgaact ttctgatccc caataccgag
      241 ctgctgaccg gcaggcggct ccagccttac tacgaccgcg ccgaccggcc gcgcatcgat
      301 gcctggcaga ctatcgtgaa cggcagactg gggctgcacg acccgaacgc gccggaaaac
      361 cgccgggcgc tggtcacgcc gtccgggctg ccgaaaacga agcaggaggc cgcagaagcc
      421 attacccggg gactgctgac ccttgcttcg tccggggagc tgaaaagccg tcaggatgtc
      481 actgacactc ttgaaggtgc aggttttgag gtggtgcgca ccacgaaaag cagtatcagc
      541 attgccgacc cggacggggg gcgaaacatc cgactgaagg gagccatcta tgagcagtct
      601 tttaacgctg gcgaaggact tagagcagca atcgaaagcg cagcagcaga gtaccggcga
      661 gatgctgaaa gccgtattca gcgagcacga gcggtctgtc agagcggaac tgagcgcaag
      721 cgcgaggaga atcagcgacg ccatcctcgc ccacgagcag agtatgagcg aggcgatgga
      781 gaaaaaccgg cggagcgtac tgcggactgc cgggcggaca tggctcacca tcctgatggt
      841 gtcagcgctg ctgatcggca cgagcgggag cattctgtgg tggcaggggc agcagataac
      901 cgacaattac acgcatcttc gtcagcagga agataccctg gcgaaaatga ccgcccggac
      961 gtggggagtg cgctatcagg agagcagcga cggccggagg tttcttatcc tgccgccggg
     1021 gatgcaaacc gaagccatcc cctacgacgg gacgacatgg atccgcctga aacaggagta
     1081 gcggaaaatg acagagctgg aaaagcagtt gctgagcgca ttagagcagc tacagcagga
     1141 ctactcgcaa aggctggacg aatgggagag cgccttcgcg gaatggcgga gcatgtgtgg
     1201 tcttatgcaa cgggagaacg cggtgctgaa cgagcgcgtg tcggacttga gcacgcaggt
     1261 gctgagttta agcgagcagc tgcgccgctt gtccgggaac tgaatgccac cgagttccgg
     1321 cagagggaga atgagtatca gaaaacactg gagctggagc gccagccgca gaaaacctac
     1381 cgcgggccga cactttaaac catccaggca gctgacaacc cgcacgaaga ggggctctta
     1441 tttctcccgc taaataggtt cgtgatcaaa aaggtcacga cttattcagg agcgggaaat
     1501 gaagctgtta aatcttattg aatcattgct taactgcctg tatctggcgg cggagctgtg
     1561 ggactggctg gagaagcatt tgtcggtttt taaggcattg gcgcggattt tagtgtgact
     1621 ttaacatcca tgcatctggc tgcatgacta tgcaccagtg taatattcag cggctgcgtt
     1681 tctggtgttt tttcgttatg tctgtcgcca ctttctcgcg tctggcaccg gaaacgcagc
     1741 caaccccgtt tcagcggtgc gggcaacgga tgttatggta aatgtagggg gagaagtgac
     1801 tataagcagg ggcttcggtg atggcctaaa gggatatgga ttattgatat tactagtttt
     1861 ctagtattta ctagtatgga tgcatcgtaa aaatcaagga acgcacaatg gcaaaacctc
     1921 catcagctac accaccggat acaccatctt catgtgatgt tagtgctctc ccaggcatta
     1981 tccaaaattt tggcttaatt aactttaacg tatcctgcaa taacggtgag ctaacggtaa
     2041 cgggtacgga tcaaaatggg aaacacataa gagttaacag ctaccaaggg aacggcttta
     2101 ccgaaggtac aatgtcaacg tttcagcccg gaggtaaaga ggccagaaga cttgaggctg
     2161 ttcgtttaag agaaagtggg cttacgcagc aagaaatagc aaaccggtta ggcgtttctc
     2221 aaaaaactat aagcaatgat ttaaaatgag tcacttgctt taagcccgtc ttaacgggct
     2281 tttcatcgct actgaaatgc catgataaat ccggcgtagt tgctcgtccc gcagtggtga
     2341 aacacctaaa gatacatttc agatgtttat tttaagccat ggtgcagatc cccatatatg
     2401 tcgaacatca ataatgatct tcttgagatc ctttccattg catcgtaatc tcttgctctg
     2461 aaaacgaaaa aaccgcctgg ggaggcggtt tttcgaaggt taagtcagtt ggggaactgc
     2521 ttaacctggt aactggcttc agcagagcgc agataccaaa tactgtcctt ccagtgtagc
     2581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatatctc gctctgctaa
     2641 tcctgttacc agtggctgct gccagtggcg ttttttcgtg tctttccggg ttggactcaa
     2701 gacgatagtt accggataag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     2761 ccagcttgga gcgaactgcc tacccggaac cgagtgtcag gcgtggaatg agataaacgc
     2821 ggccataaca gcggagtgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     2881 gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2941 tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt
     3001 gccgcggcct tatcgtttcc ctgttaagtt ccttcctggc atctaccagg aaatttccgc
     3061 cccattcgta agccggtacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3121 gcgaggaagc ggaatatctc ctgtattgca tctcctgttg acgcgccggt gccacctttt
     3181 ttctcctgcc acatgaagca cttcactgat ttccgcatcc atgccaacat agtaagccag
     3241 tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac ccgccaaaag
     3301 cctgacgcgc ctgcggcttg tccaaccccg cgccgaccgg gaaaggcttt cccgcccgtc
     3361 ccggcgttat caggaggccg gtttgtcaga gaaacggagc aagatgctca ccatgtgggt
     3421 gacggaggac gagcaccggc ggctgctcga gcgctgtgac ggcaggcagc tggcggcgtg
     3481 gatgcggcag atctgcctgg acgaaaagcc ggcacgttcc ggaaagctcc cctcactctc
     3541 gccggcgctg ctgcgccagc ttgccggcat gggcaataac ctgaaccaga tagcccgccg
     3601 tgttaacgcc ggcggcggca ccggccatga cagggtacag attgtggcgg cgctgatggc
     3661 catcgatgcc ggccttgagc ggctgcggca cgccgttctt gagaaaggaa cggacgatga
     3721 tcgttaaatt tcatccgcgc ggccgcggcg gtggcgccgg gccggtggac tatctgctgg
     3781 gtaaagaccg gcagcgtgaa ggcgccaccg tcctg
//
