LOCUS       NZ_CP115677             4911 bp    DNA     circular CON 24-FEB-2024
DEFINITION  Enterobacter hormaechei strain 2020CK-00203 plasmid unnamed5,
            complete sequence.
ACCESSION   NZ_CP115677
VERSION     NZ_CP115677.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN15907138
            Assembly: GCF_027886505.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Young,E.L., Hergert,J., Casey,R. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 08-AUG-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/24/2024 04:17:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,975
            CDSs (total)                      :: 4,857
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 26 of 68
            Pseudo Genes (incomplete)         :: 38 of 68
            Pseudo Genes (internal stop)      :: 14 of 68
            Pseudo Genes (multiple problems)  :: 10 of 68
            Pseudo Genes (short protein)      :: 1 of 68
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115677.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="2020CK-00203"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2019-03-02"
     gene            complement(<417..1121)
                     /locus_tag="PF324_RS24895"
                     /pseudo=""
     CDS             complement(<417..1121)
                     /locus_tag="PF324_RS24895"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1060..1383)
                     /locus_tag="PF324_RS24800"
                     /old_locus_tag="PF324_24800"
     CDS             complement(1060..1383)
                     /locus_tag="PF324_RS24800"
                     /old_locus_tag="PF324_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2022..2192
                     /locus_tag="PF324_RS24805"
                     /old_locus_tag="PF324_24805"
     CDS             2022..2192
                     /locus_tag="PF324_RS24805"
                     /old_locus_tag="PF324_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            complement(2401..2733)
                     /locus_tag="PF324_RS24810"
                     /old_locus_tag="PF324_24810"
     CDS             complement(2401..2733)
                     /locus_tag="PF324_RS24810"
                     /old_locus_tag="PF324_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(2901..3116)
                     /locus_tag="PF324_RS24815"
                     /old_locus_tag="PF324_24815"
     CDS             complement(2901..3116)
                     /locus_tag="PF324_RS24815"
                     /old_locus_tag="PF324_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3343..3531
                     /locus_tag="PF324_RS24820"
                     /old_locus_tag="PF324_24820"
     CDS             3343..3531
                     /locus_tag="PF324_RS24820"
                     /old_locus_tag="PF324_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            3541..4740
                     /locus_tag="PF324_RS24825"
                     /old_locus_tag="PF324_24825"
     CDS             3541..4740
                     /locus_tag="PF324_RS24825"
                     /old_locus_tag="PF324_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     ARG             complement(2402..2733)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(1480..1671)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          1651..2134
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(4..1070)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2804..3231)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.828"
                     /coverage="100.0"
                     /evalue="3.770000000000001e-64"
ORIGIN
        1 gtacgccgct gttcctctct gacagcatgg cctgcgtgct cttctgctcc cggatggtcg
       61 tatagttctc gagtatctgc tgcccctgcc accacagaat gcccgcgctc gacgctatca
      121 gcagcgcgga gaccagaacg atggtcagcc acgtctggct gaccatgcgc agcatccctt
      181 tcgtgcgctg gctcatggct gaggacagct tccggtcgtg gtcgaggatg gcggcgctga
      241 ttctcttctc gctttcgctc agttccgctc tgacagactt ctcgtgctcg ctgaatgcgg
      301 ctttcagcat ctcgccggta gtctgctgct gcgctttcga tttctgctct aagtccttcg
      361 ccagcgttaa aagactgttc atagatggct cccttcagtc ggatgtttcg ccccccgtcc
      421 gggtcggcaa tgctgatgct gctttttgtg gtgcgcacca cctcaaaacc tgcgctttcc
      481 agcgcctcag tgacgtcctg acgcgttttc agctccccgg atgaggcaag ggcaagcagg
      541 ccgctcgtaa tcgcctgggc ggcttcctgc ttcgcttccg gcagcgcgga aggcgagacc
      601 agcacccgcc ggttctccgg cgcgttcggg tcatgcagcc ccagcctgcc gttcactatg
      661 gtctgccagg catcgatgcg cggacggtcg gcgcggtcgt aatacggctg tagccgcttg
      721 ccggtcagaa gctccgtgtt cgggatcagg aagttcagct ccagccgccc cttgtcccgg
      781 tgctcaaccc acagcacgct gtactggtct ttatcgagtc cgggcatcag aacccgctcg
      841 aaactcgcca tcagcttttc gcgctggccg ggcggtaaat cctgttcggc aaaagacaga
      901 acgccggaag tgtacttttt ggcgtagggc gaggcgtcga taagctcccg gacttcatcc
      961 ggcttcccct gcagaacgct ggcgccgtcg cgctggcgat ctttacccag cagataatcg
     1021 accggcccgc cgccgccgcc gcgcccccga ggatgaaact taacgatcat catcagcacc
     1081 cttttccagt acggcatgcc gcaaccgctc gagtccggca tcgatggcca tcagcgcggc
     1141 gacaatctgc acgcggtcgt gtccgcttcc accaccggcg ttaacctggc gggcaatctg
     1201 gttaaggttg ttccccatgc cggcaagctg acgaagcagc gccggcgaga tcgacggaag
     1261 tttgccggcg cgtgcgggct tctcgtccag gcacgtctgc cgcatccagg ccgcgagttg
     1321 cttaccctcg cagcgctcca gcagacgccg gtgctcgtcc tcagtcaccc acatggtgag
     1381 cattttgctg cgtttgtctg ccaatccggc ctcctgataa cgccgggacg gacgggaaat
     1441 cttttcccgg tcggcacggt gttggacaag ccgcaggcgc ggcagtggtt ttagcgggtt
     1501 tcggggcgca gccctgaacc agtcacgtag cgctagcgga gtgtatactg gctaaacatg
     1561 ttatgacgga gtggcaattt atgaaagtgc ttcatgtgac aggggaaaaa tgcggctgcc
     1621 gggcgtaagt gcaaaatgca ggtggtgatg aaatgacgcc tcctcgctca ctcggtcgct
     1681 acgctcctgc cgtgagactg cggcgggcgt taccagctca cagattacgg gataagcaaa
     1741 tgccgctcga agcaggaaag gccaagaaaa ggatgcggag ttgccgtttt tccataggct
     1801 ccgcccccct gacgagtatc acgaaaatga cgctcaaatc agcggtggcg aaacccgatg
     1861 cggcttaaag ataccaggcg tttccccctg gtggctccct cgtgctcccc cgcattcctg
     1921 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
     1981 aactcggttc cgggtaggca gttcgctcca ggatggactg tatgcacgaa ccccccgttc
     2041 agtccgacta ccacgcccgt tccggtaact atcaacttga gtccaacccg gaaagacacg
     2101 acaaatcgcc agtggcggta gccattggta actgggttca cggagcagag attgtagaga
     2161 atgcctacag agttcttgaa gtgaaagcct gagtgcggct acactgaaag gacagtttag
     2221 gtatctgcgc tccgtgaaag ccagttacca tcggaacaaa gagttggtag ctctgaattc
     2281 cggcaaacga accaccgttg gtagcggtgg tttttttgtt tacagggaaa aagattacgc
     2341 gcagaaagaa aggatctcaa ggaaatcctt ttgctttggc caatatgctt cggtaaaaag
     2401 ttaatgcgca gaagattttg aaaaaatatt aataaccaaa actccaacgc aaataaggaa
     2461 cattcctgca attgcgggcc aatcaagttt ttgaccgcta accaaccaac cagctaggct
     2521 tattagtaca attcccgcac cagaccaaat tgcgtaagca ataccggtag gtatattttc
     2581 caatgtttgg gaaagcagat aaaaggcagc gcaataactt attgcagttc ctacagacgg
     2641 ccacaggcgt gtaaatcctt ctgaatattt cataaaggtg gtgcctatga cttcagccac
     2701 aatagcccct cccaggaaaa tatatgtatt cataaaaata cctcatgttg aaaaatttaa
     2761 atatctttac ataacaacta attaatagaa cccggcttat catagcgaag gaccatgatg
     2821 ctgccactgc gatcgttcca gctccagcgc tttctggtgc tgcaccgccc gctcctgctg
     2881 ccggtgagtt tctatcgcgt tcagcctttc gacaaccggc gcagctgctc gctcaaactc
     2941 tgcacctgcc tgctcaagac cgtgacgcgc tcgttcagcg ccgcgttctc ccgttgaata
     3001 agaccagaca tcgtccgcca ttccgcgaag gcgttctccc actcgtccag ccttttcgag
     3061 tagtcctgct gtagctgctc taatgcgctc agcaactgtt tttccagctc cgtcatgtgc
     3121 tatttccccg ccagtatcat ccagctcgag tcctctccga actgtcccgc ttccgggttc
     3181 accctcacgc agcggcgtct ctgctcgccg caggtcgaga gctgaaccac ggcagcctgg
     3241 cggctgcaaa ttgaaaaccg ccccggttcg tcctgttgtt gccgcacgcc ccgcagaata
     3301 tgtatgctct gaggacctgt tttattaagg actcatcccc ttatgttgac ccgcaaaagt
     3361 atcgatacag ttctgctctc tgttggtgct gagaagctct cacaacgaga atgggactgg
     3421 atgaaaatgc tcaaacccat ggacccacca cctgcgatgg tgacgacctc catcctgaag
     3481 cgccgtggcg atacagcagc ccttaccctg ctacaggata ccggggttta atccatcctc
     3541 atgttgtccg gttttactcc ccgccctctg aaacgtctgt tcacagccaa ccagtgctgg
     3601 acgtccttcc tggatgcggg cggtctgcgc gatatcgagg ttgaagccgt caccaaaatg
     3661 ctggcctgcg gcacacggat actgggtgta aaagagttcg ggtgtgataa cccggactgt
     3721 cagcacgtaa agtacctgac caactcatgc ggcagccgtg cctgcccgtc ctgcggaaag
     3781 aaggccacag acctgtggac agcaacacag ctgaatcgtc ttcctgactg cgactgggta
     3841 catctggtct tcaccctgcc ggacacgctg tggccggtgt tcgaaagcaa ccgctggctg
     3901 ctgaatgacg tgtgccgtct ggcggtggag aatctgctgt atgccgcccg aaaacggggg
     3961 ctggaacccg gtatcttctg cgccatccac acgtatggcc gtcgtctcaa ctggcatccg
     4021 catgtacatg tgtctgtaac ctgtggaggt ctgaataagc atggtcagtg gaaaaagctg
     4081 agcttcctga aagacgcgat gcgttcacgg tggatgtgga atatgcggca gctgcttctg
     4141 aaagcgtggt cagaggggat ggcaatgccg gagtcgttgt cacatatcac gacggaatca
     4201 cagtggagaa gcctggtgct gaaatccggc ggaaaatact ggcatgtgta catgtcgaag
     4261 aagacagccg gagggcggaa tacggcacgc tacctgggtc gttatctgaa gaagccacca
     4321 atagcggctt cccgactggc tcattacaac ggaggggcaa gcctgagctt ccgttacctg
     4381 gaccacaaaa cgggagaaac ggcgacggaa acgctgacac agcgtgagct ggtcgcgagg
     4441 ctgaaacagc acatcccgga gaagtttttt aagatggtga ggtacttcgg gttccttgcc
     4501 aaccgtgtgt gtggagagaa gctgccgcag gtgtaccgtg cactggggat ggataaaccg
     4561 gaaccagtgg cgaaagtgtg ctatgcacaa atggtgaaac agttcctgag tcgtgacccg
     4621 ttcgaatgcg tgctgtgtgg ctgccggatg gtataccgcc gggccattgc ggggctgaat
     4681 gtatcagggc tgaagaaaaa cgcgcgggat atcagtctac tgaggtatat gccggcctga
     4741 cacaggctaa gtgtgcccgg gaatcggtga aagtataaaa aacagcctgt tacggtataa
     4801 aaatcgacca tacagagagg gaaaccaccg gagtggtggg aaaagcagaa aggctcacgt
     4861 ggcataaggc gtggacaatg gtatcgactg gtttttcaat ttcctatacg t
//
