LOCUS       NZ_CP115620            10123 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Citrobacter freundii strain 2022CK-00369 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP115620
VERSION     NZ_CP115620.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29151494
            Assembly: GCF_023756145.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00369
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2022) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 10123)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:50:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,794
            CDSs (total)                      :: 5,667
            Genes (coding)                    :: 5,551
            CDSs (with protein)               :: 5,551
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 27 of 116
            Pseudo Genes (incomplete)         :: 88 of 116
            Pseudo Genes (internal stop)      :: 20 of 116
            Pseudo Genes (multiple problems)  :: 18 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115620.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00369"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA"
                     /collection_date="2022-05-29"
     gene            complement(728..877)
                     /locus_tag="PF691_RS28910"
                     /old_locus_tag="PF691_28895"
     CDS             complement(728..877)
                     /locus_tag="PF691_RS28910"
                     /old_locus_tag="PF691_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_010891188.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(962..1219)
                     /locus_tag="PF691_RS28915"
                     /old_locus_tag="PF691_28900"
     CDS             complement(962..1219)
                     /locus_tag="PF691_RS28915"
                     /old_locus_tag="PF691_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_010891189.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(1229..2914)
                     /locus_tag="PF691_RS28920"
                     /old_locus_tag="PF691_28905"
     CDS             complement(1229..2914)
                     /locus_tag="PF691_RS28920"
                     /old_locus_tag="PF691_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032427631.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3241..3486
                     /locus_tag="PF691_RS28925"
                     /old_locus_tag="PF691_28910"
     CDS             3241..3486
                     /locus_tag="PF691_RS28925"
                     /old_locus_tag="PF691_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515249.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3608..4174
                     /locus_tag="PF691_RS28930"
                     /old_locus_tag="PF691_28915"
     CDS             3608..4174
                     /locus_tag="PF691_RS28930"
                     /old_locus_tag="PF691_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008812849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein K"
                     /protein_id="WP_010891191.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
                     TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
                     EGVIYNYYLSDKLIYTYKALPSICS"
                     /name="protein K"
     gene            4260..4523
                     /locus_tag="PF691_RS28935"
                     /old_locus_tag="PF691_28920"
     CDS             4260..4523
                     /locus_tag="PF691_RS28935"
                     /old_locus_tag="PF691_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_010891192.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="protein L"
     gene            4869..5132
                     /locus_tag="PF691_RS28940"
                     /old_locus_tag="PF691_28925"
     CDS             4869..5132
                     /locus_tag="PF691_RS28940"
                     /old_locus_tag="PF691_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515247.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5122..5421
                     /locus_tag="PF691_RS28945"
                     /old_locus_tag="PF691_28930"
     CDS             5122..5421
                     /locus_tag="PF691_RS28945"
                     /old_locus_tag="PF691_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_021515246.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5483..5860)
                     /locus_tag="PF691_RS28950"
                     /old_locus_tag="PF691_28935"
     CDS             complement(5483..5860)
                     /locus_tag="PF691_RS28950"
                     /old_locus_tag="PF691_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515245.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     gene            5929..6108
                     /locus_tag="PF691_RS28955"
                     /old_locus_tag="PF691_28940"
     CDS             5929..6108
                     /locus_tag="PF691_RS28955"
                     /old_locus_tag="PF691_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6136..6666)
                     /locus_tag="PF691_RS28960"
                     /old_locus_tag="PF691_28945"
     CDS             complement(6136..6666)
                     /locus_tag="PF691_RS28960"
                     /old_locus_tag="PF691_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021515243.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(6673..7404)
                     /gene="mobC"
                     /locus_tag="PF691_RS28965"
                     /old_locus_tag="PF691_28950"
     CDS             complement(6673..7404)
                     /gene="mobC"
                     /locus_tag="PF691_RS28965"
                     /old_locus_tag="PF691_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004146447.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7404..9365)
                     /locus_tag="PF691_RS28970"
                     /old_locus_tag="PF691_28955"
     CDS             complement(7404..9365)
                     /locus_tag="PF691_RS28970"
                     /old_locus_tag="PF691_28955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_021515242.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7914
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          45..625
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6677..7404)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="100.0"
                     /evalue="7.29e-144"
ORIGIN
        1 caaagttacc cacagattcc gtggataagc aggggactaa catgtgaggc aaaacagcag
       61 ggccgcgccg gtggcgtttt tccataggct ccgccctcct gccagagttc acataaacag
      121 acgcttttcc ggtgcatctg tgggagccgt gaggctcaac catgaatctg acagtacggg
      181 cgaaacccga caggacttaa agatccccac cgtttccggc gggtcgctcc ctcttgcgct
      241 ctcctgttcc gaccctgccg tttaccggat acctgttccg cctttctccc ttacgggaag
      301 tgtggcgctt tctcatagct cacacactgg tatctcggct cggtgtaggt cgttcgctcc
      361 aagctgggct gtaagcaaga actccccgtt cagcccgact gctgcgcctt atccggtaac
      421 tgttcacttg agtccaaccc ggaaaagcac ggtaaaacgc cactggcagc agccattggt
      481 aactgggagt tcgcagagga tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc
      541 caaagtccgg ctacactgga aggacagatt tggttgctgt gctctgcgaa agccagttac
      601 cacggttaag cagttcccca actgacttaa ccttcgatca aaccacctcc ccaggtggtt
      661 ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg
      721 atcttttcta ctgaaccgcg atccccgtca gttcagagga ggacgatggt gccaccgttc
      781 caccctgaac atcccggata tagtttgcct gacatgcgac cagaagaaag cctgaaacta
      841 tcaatataaa aagaaaaata gcctttgcct ttttcatata ccttccttgt attcagaggc
      901 ttcggcattc atccgtgaac accggaagcc tgtatttcaa ctcatccttg ataacttttg
      961 cttaccagtt accatcccgg taaacaaagg atacaaagta atcaaattta ctaatatcga
     1021 ttacattttt aaagtgtggc tgtaatatcg aaacccatgg tttttcaaca tcaaaccaac
     1081 cattattaat attatccttt aaaggcatcc ccagactttc aatgacagaa ccatcatcac
     1141 cgaagtcttt tgagtattca ccgcctttaa actcttcggt tttcttatca aaccaatgaa
     1201 tatgtaattt aagccccata tttacctctt aaagatattt tttaatgttt cgttttggat
     1261 ccggcccttt aacctgctta cccgtttttg gatcgaatgc gccgaggtgt tcgccatcac
     1321 tggcgcggta tccttcaagc tcaccgtgct gggaatccca ttcataaatt ttacgctttt
     1381 tatcaccata ccatcgggcc cgcttaccac ctcctccttg tttaggggtt ttaggttttc
     1441 cctctttcaa ctctcccaac cccttaatat cttcagtctt gggatcagga aaataatcat
     1501 ggccataatc tttagttccc ttgcgaggct ttttcttttc ctcatttaat ttattttctg
     1561 cctccttcgc ttttttctcc ttttgtttcc ggctctccat cgccccggaa agcgcagcct
     1621 tatctctgga taaggcatca ttcattgcgt ttaaagatgc ctgagcagct tttaatttcg
     1681 cattctgatt ctgaatatcc ttatcaagat agcgaacctg atcctgccac ccggagccat
     1741 attcatgagg agggttataa gtcacaaaat catccttatt cgcctccgcc tctttcacct
     1801 ttttatcagc ttcctggaca gcagccctgg cgccaggaat cgctttttct gtcgatgtaa
     1861 tcctgccgtt taaagtcgta atatttttat cttcggcatc cagctcagct ttcgctttat
     1921 catattctct tttcagcccc tcttccggat gagcggcgtc ccatgcctgc tggcgacgct
     1981 tttcttcctc ctgacgctgt tttacctgtg ccggggtaag aacatctgtc accgacacat
     2041 agaccggctt ctgtccggtc tctttcggga aacgaataac ggcctcacgg gagttaccac
     2101 cggccgtaaa accagccgga cgtgaatcac ctttttcagc aacaatgcct tttggcgggg
     2161 ctttcgctgc cggaacacct ttcggtacgc tcacctgcag agccgggaga cccggaatag
     2221 acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacagga acactcattg
     2281 gccgacccgc gacaaccgca atatgctgcc gctcatcctt caccacatcc acaacccgtt
     2341 gtctgacacg aaccgtcgct tggtccagcg gtaaagtact tgccggagtc tccgtcaccg
     2401 tatcggccgg taatgacgtc ataatttttg acatcatttt cggatcatct tttgcaatct
     2461 cagaaggcag tacaccatag atcgcaatcc cccacagacc aaacttaaac ggacctttca
     2521 gggcagccag cacatcggca acggctgacg acagcgcatc gccggaaaag gataaagcca
     2581 gtccttcagc ccccggtgta gccagggcag gcagaccgaa ggccatcgcg gtggccgaag
     2641 actgtccacc accagagcta ccgctatgat tgccgctgtt accaccatta ccaccgggtt
     2701 tactcccgcc gttaccaaac tcccccggat tatagttatg aatatctcca ttcggcgtat
     2761 gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc accacctgat gaagggccac
     2821 ctttaccaga cgtaccgttc acattcccac tggcctgacc accattatgt cccagacctg
     2881 aattacccgg acctcgaccg tctccaccac tcatatcgtt ttcctctttg acatttaaaa
     2941 caataaatta aaaccaaaat actgtatata tacacagtat tattattacc ttactcctcc
     3001 agttttcagg tgtcaatata tgagattagt tctcatcttc aggcacaaaa aagcgcccgg
     3061 agccgcctgt aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac aaattttatt
     3121 atttgagata ttcatcacac ttacttttat attggtatag atgtacctga aagcgaaatg
     3181 gcatgaggag aggacctgcg gttaaactgt tcgggaaacc tgagacgccc ggaggccact
     3241 atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct
     3301 caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag
     3361 gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa
     3421 atgcgtcggg agaaatacga ggcggcggaa accaccttca cctggcagcc gcagcgccgc
     3481 cggtgaaaac gatttaaggt tctactttca ggtgtatcca cttcggaagc attttaggga
     3541 tatggtatta tcgaaaatca tgctttcggc aatccaatgg gtttatacga tttaaaaagg
     3601 gaaaaacatg gacaaaagga caacgttagt agctctggtt tctatcatct ttttcacttc
     3661 tggatgtgat gaccagaaaa aagggaatgg caacgctgaa tcgacacaaa tcactaaaaa
     3721 cgatataaca actcaactta agttagataa aacgcaacaa cctgacgata tagaaaagaa
     3781 aataaaagaa atccaggcca actccacccc tgaaaaaata aaaaaattca atgaattaaa
     3841 agatgacgac cctaaaatcc tacgagagtc ggttgtttca gaaattaaga aaaaactacc
     3901 actattggtt gacgaagcca cattaatgac agatgtatca actgacggag gtacattctc
     3961 atacaaatat gtaaccaaag ggatttcagc cagcacgatg gaaagtgatg tgtggaaaga
     4021 tgccatgcaa aaaaacataa aaaatagcta ctgctctgac gatgccagac taaaagtatt
     4081 cagagagcta tttcctgaag gtgtcattta caactactac ttaagtgaca aattaatcta
     4141 cacatacaag gcacttccat cgatttgttc ctaaagtatt taccataacg tcgcaagctg
     4201 ggcgccccag cgggcggctt ttctatatct gtcctataaa ttcagaaaag gaaagctaaa
     4261 tggctgcata taccagcgaa acagcgaagt ttttacaaag cgtcagtagc gatcaaacat
     4321 ggtggaagtc acgatataat ccaggagaca gcgttcccca ttctggcatt tatcgatgta
     4381 cagtctgcgg aaaagagatc accagtaacg gcaacgaccc gttcccgccg caaaaccacc
     4441 atcagcacag tcagcaacaa gcaatcaaat ggcaactgat tgtcagaacg gacactaaag
     4501 gtgaccgttt cggaatcaaa tgattgagag ccatccggag ctttttttgt acctgaaact
     4561 cgaagatatt taccataaca tcccttatcg gtaccgccag taaggcactg gcggcgtttc
     4621 cggcggctac gggataaaac aagcaacaac ccacccggaa aaccgccgga aacgcgcccg
     4681 gtaaatttta tcctaatgca tgcctatgca cgcaactgca tagcatatgt tttgacgtaa
     4741 aatggatgcc gtaagccctt tttttaccga ctttctgctt catcagcctg actgacacga
     4801 ccatattctt tcagaccgcc ccctgctaaa cttcttcata tcaccgcatt aacccgggag
     4861 gctccgcaat gagcaacact acacactacg aaaacgccaa cttcctgcgg gaactggctg
     4921 aaaacctgcc ccgcatactg ccggaaggtg gcccggacaa agctgcgctg ttgcaacgcc
     4981 tggctaacga agaactggcc caggcagagt atgaagatca ggttcgcgct aaagtgaccg
     5041 ccgcccgcgc tgacacgcgc tctggcatga ccacggaaca acttcgccag agactgcatg
     5101 gacgctatca ggaactacgc gatgccgtat gaggtggaat ggaagcaggg agccatcgag
     5161 gatgtgacag cattgtttga ctacattgcg gaaaattcat ccctgtggga tgcgcagaac
     5221 gttaccgatc gcgttctcgc tgcggcggac aaactggccg acttcccgcg cctctatgaa
     5281 gtggactcac gctatggcga ggacgttcgc cggatcagcc tgatgggaca gcatatcctc
     5341 tatgatgttg atgaccaggc ccggaaagtc cgggttctgg ctgtggtcgg ccagcgccag
     5401 aatccccata ccgttagcta ataaccgatg acgggggcgc aattcattgc gtggccgtcg
     5461 ccggttggac aagcgcagcg cgtcagcgtt ttccccagtg ccaccaccgg cgcgacgttt
     5521 cgggatgtgt atctgctgtc tccggtttgt gttccagcag tctcatggcc tgcatttcct
     5581 gccgcagtcc ggctacctct tcccgcagcg ctttaatctc cgaaatcagt gcctcagaca
     5641 ccgatgcttc agtctcaccc ggcagtctca acccgtcccg ctcctgagtc tcaccctgtc
     5701 ttaattcacc gtacacccgg ataagctcgc tggtatccac cacccgacca ccatcagctt
     5761 ccgtacggta tgatacgcgg cctttcgcca tatcacgata cagagaactc cgggctttcc
     5821 ccgtcagttc cgtcgcctgt ctcaccgtca cccacgccat tgtctcacct ccggttttga
     5881 gacagtatac ccgacaggaa gcacaccaca cccggaagga cagaaaacat gacgaagcag
     5941 gaaaaaactg ccctcaatat ggctaaattc attaaggacc agtcgctgct gttgctcgag
     6001 aaactgaacg agctggatat ggataccccg gccgacatgt gcgagcggct tcacgaggat
     6061 gcggaaacac tctggcaggt gatgcgggat aagctcggga aggaataaca gcaccggcca
     6121 tgccggtgct gccgtttatt ttttgccgga cggccaccac cggcgggcgg gtttgtcctc
     6181 cggcagttca ggcaccggca cgttaaaata gcggctgtta tattcccgca tcagctccgc
     6241 tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg cttaccacct caatcatcgc
     6301 gttaatctgc tccggtgagc acttcccggc atcaaccacc tgcacaccgt tctcgatggc
     6361 gaaccgctcc accttgcgat agaggtaatc actcagcttc attcctgtgg gtttgtcccg
     6421 ttcccattgc ggattttcac cagtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg
     6481 ctcgtcatac atcccgatgg atttcatcat cgccagatgc tgggcccggt cgacaaaaca
     6541 gaaaacatac gccccggtct gccaggcgag caccaccata tcttcaagat gctcgctggt
     6601 cggcatgatg cagcgctcgc cgtcccggtc ctccgcgtct tcaataaata ccatgtacag
     6661 cgccataatc cctcactccg ttgtcataaa tttaaagcac gcaaacagcc gttcacgatc
     6721 cagctccgcc gttttgcggg cctcgccggt ctgtggattc acgatttcat ccaccgacag
     6781 gatcgcccgc tgcaccctga cctgcgtcgc ccggtccggg cagatgtacc agatttcatc
     6841 gatgccgttc gcccggcggt taaacagata cccggacacc acctcctgat agcgcctgcg
     6901 gctcttcacg gtgcgctcca cctcaaaagc caccgttctg ccgtccagct ccgccagcgc
     6961 gtccggcacc ttcatccctt tcagggcaat acggtgcagg taccgccagc cggtggcccc
     7021 ccgggcctcc atggcgaggc gcagggactg cacggccagc cggtgcggca gctgcgccgc
     7081 cgacacccgc gacggctgaa acggaataat gtccgtcagc ggctcgtcct cgtcgaacga
     7141 gaacgccacc ccgtgcggcg tcagccccca cacgctgacc tgacggccgt caaccgggta
     7201 tttcgcccgg accagcagcc ctttcgccac caggctgtcc aggaagcggt aaaccggctg
     7261 taccgccgca atccccagcc gtcgcgcaac cagctccgcc gtggaacaca gctcttcccg
     7321 cagccagcgc agggtcagcc gtgttttctc cgcgttccgc gcctgccgct tcgcgtggct
     7381 gacgttgtat cgctccagtg ccatcagtac agccccgcaa aatcatcatc acccggccgc
     7441 tgccgcctgg cctccggctg gtactccgcc agcgggtccg ttttcgccag caccgggaag
     7501 gttttcagcg gaatttcccg gcgatcaacc cgcagcgggc tgctgaacgc cagccgcgcc
     7561 acgcccagcc cggtcatcac cgcgaacccg tcaggcagat gttgcagggt gttcacgtca
     7621 aacagcggcc gcgcctcctt ctggatgaag ctgtccccgc tgatatgctc cacgttcccc
     7681 gcctcccggc tggcccggcg gcgctccacg tccacccgga tttccccggt ctggcccgcc
     7741 gcccactgcg cggactcctg gctggccgcc cggtagaacc agcggatcgg cgtgttatcc
     7801 agcaccgtgg ttttcacaaa atcagcgggc agatcctggc cgcactgccc gaagtcgccc
     7861 agcgactggt gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc
     7921 agcacgtact tcgacagcag gtatttaatc tcatcgagca tgatgctcgc gtgcggccac
     7981 ctccggaact catcccgcgc aatgatgatt tgtgcgcagc gggcaaacag catcttctgc
     8041 acccggatca ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg
     8101 attatccccg ccacgtcgat gccgctgtcc gtctgcagga cgctcaggtc ggccacgttc
     8161 tccagcttgg tgatcagccc cttcacgtcc tttttcaggg cctccggcag cgcgtacgcc
     8221 gccgccagca cctgctgaat gtttgccccc tgcggaaact gctccgcaat cagcttcgcc
     8281 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac
     8341 ccggcaacca gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc
     8401 gccggtttcc ccggccgcag atccagcagg gtgaagtttg ggcagtgcgc ccggaagacg
     8461 tgcggcgccc acgcgtcccc tttggggtcg aagtacacca ccgcgtcccc gaaatcgcgt
     8521 acgcagcgaa tgagcgcgtt ggtggccatc acgctcttgc cgctcccggg caggccgagg
     8581 atctgaatgt tggtcttgcg ccaggtatca cgcgggacca gcaccggctg gccctgctca
     8641 tccttaccca gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc
     8701 gcgttgaccg gtaccggctc cccgaaatgc aggtggcgga cgtcggtcat ttcaccggtc
     8761 cgctggctgc tgcgccggct ctcctggttc agtttcgcgt tcaccgcctc gtcagcagcc
     8821 gcaaaatgac cgtcaagcag caggaacgcc agcagccgcg ccaacacaat cccgacaacc
     8881 agcccgcccg cgcaccaggc aatcccgaac aggttagccg gctgcagcag gtaataatct
     8941 gcgttcgcgt agaggtccgg acgcaggacg gtttctcccc agctctcgat aaacacggcg
     9001 acaaccagcc cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc
     9061 cagacatgcc gccgtgccct gaccatccgc ccgggcgaat ccagcgaggc cagcaccgaa
     9121 aatggcgcag tccggttatt atcgcgctta tagcgatcgg catactcccg tcgctcctta
     9181 tgactcagcc ccacaaaacg catccgtccg gcccagagtt cagccagacc gaaaaacagg
     9241 ccgataacca ggaaagcccc ggccagaccg taatactccc tgtaaagcca gctgacccac
     9301 gggtaaatct gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta
     9361 aacatgtaat ctctctcctt cagacgacgg gtttaccggc gtggtgtgca gcccccgctg
     9421 cacaagccgc ggacatgccc caagggatgg cggcggcgtt cacgtccgtg gtgactgcgt
     9481 tcagctttca ccacggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg
     9541 aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt
     9601 tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc
     9661 agaggcagct ttgctgcgac gtcaggtgcg tccagcaccg tggatttttg tgggtggtcg
     9721 ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga
     9781 gccagaaaga gggggaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaggggatt
     9841 tccccctgaa gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa
     9901 aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc
     9961 ctgataattg atcgtcaggg aatagaaaga cgtaatcagg ggacaatatc ccacatcagc
    10021 gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc
    10081 tacgctccgg gcgtgagact gcggcgggcg ctgcggacac ata
//
