LOCUS       NZ_CP115389             2104 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET20-PYO2 plasmid pCUVET20-PYO2.3,
            complete sequence.
ACCESSION   NZ_CP115389
VERSION     NZ_CP115389.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339838
            Assembly: GCF_030996785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:10:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,009
            Genes (coding)                    :: 4,683
            CDSs (with protein)               :: 4,683
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 179 of 326
            Pseudo Genes (incomplete)         :: 166 of 326
            Pseudo Genes (internal stop)      :: 42 of 326
            Pseudo Genes (multiple problems)  :: 57 of 326
            Pseudo Genes (short protein)      :: 4 of 326
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115389.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET20-PYO2"
                     /isolation_source="Endometrium"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET20-PYO2.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2020-07-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(591..1554)
                     /locus_tag="PCS90_RS25635"
                     /old_locus_tag="PCS90_25575"
                     /pseudo=""
     CDS             complement(591..1554)
                     /locus_tag="PCS90_RS25635"
                     /old_locus_tag="PCS90_25575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     Typing          complement(932..1161)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.142"
                     /coverage="100.0"
                     /evalue="1.59e-114"
ORIGIN
        1 aacggacttt aaaaaacggt gaacggatgc ggattttaat ccgtgtctgt gaggcgttga
       61 gcaacgagca aagcgaggcg gtagaaagag agccagcggc aggctcagga cgataatccg
      121 gggaaaatgt accactcgca gagtgttaac atttgacatc ggattgtcta tcctgcattg
      181 gtgtcgagtt ggcaaggctg aggataatgc tgaggataat gctgaggata atgctgagga
      241 taatgcagga tagacagagc actagagatt gcatgcaatc tcgtacctat cacctcagcc
      301 tgccgctggc tcttttccag aactaagaat gcgttcttaa aaatccaaac ttgataccgt
      361 ttaaatctat tttcacaaaa agagttttta ccctctcgtt tgttttctga atatataaaa
      421 ttaattcatc ctcaaaacct tcgtgcggat gaaaaccaaa tgtgccaaaa cttaatccat
      481 caataagcaa aataaggttt aaacctgtca ggaatcaccg cttcacttct taaaaaaaat
      541 aaaccaatag tcttatttcc attcaggtat tcaatattaa tattcatacg ttaatccgat
      601 ttatccttct caggggcgcg tttgtaacgc cgtttacctg aatcccagac aaacgccgtc
      661 cgcttcccgt catcagtgcc atcccctaca tcgtcggcaa tgacaagatc ctcattggtt
      721 tctcgatcca actggaggac gttttttagc gccccaccgg tcgagataaa acggcgcttg
      781 tgaagctgcc gcgtcagctc aagaaaccac tcaggatcgt ttgccatatc ttccggttta
      841 acggagtatt tcagcgtttc agcaaccgcg ctttgcagtt gctcggcaac gttggccaca
      901 acctcacctg tcttagtttt tactgcccga atatcgatat tcggctcata gttcacccgc
      961 aagcaatcgc gccagagttc tacccaacgt tcgcttaacg tagttcttcc ctttaaacca
     1021 agaaggttgc accatcagca gacagtgaaa atgcggatgt gcgctgccat ccttacctcg
     1081 cgtcacctcc gtagccctga tccacccctg aacaggtgat agctcctttc gcttttccat
     1141 tcgcttaaac gccgcattca ttgctgtaag gactgttcca agttcaccta tctcgcagtt
     1201 cctgacagtt aacgtcagaa acaaccatcg ggaagacggg taatccacaa cgattttcgg
     1261 tagagcctga taaaaacggg cttgccacat gagggttctt ctccactggc agacagggca
     1321 atgacggaca cggcaaaatt gcgcactacg aagccgtagg cgcgtttctc cggtttccat
     1381 gatggtagac caaccaaaac gtaacaaacc gctacaggag gccatacgga gtgcatagcg
     1441 ctcgaattca gcagcagtct ggtagatacc cccgacagaa tcggtaagcg tgcgctgggt
     1501 atcccaaact gcatcacgga ggctgtagtc cgaaaggaat ttatcttctg acattaagcc
     1561 tgctccgatc tgggcaggct gtgatttgat gtggtatatt catcacgtac ctgtgcattg
     1621 cgagccagta catgtcacca gagtttgccg ctgaggtgac acattcgaaa tgttggggag
     1681 tgttgacgca ctccccaacg ccgattaaaa acgcctcaat tctgctccat tttctttgac
     1741 ctgtcaaaac ccactcaaag cctcgtcatt actgggctaa accactattt catatccgcc
     1801 atttatccct taagtataaa tacaaggatc acgcgcgaga attgcgcaaa aaataaccac
     1861 aattttaacc ccccaaaaca agcacccccg aacggcttac cccaaccttt tccacacacc
     1921 cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg gcctcacttc
     1981 gtgaaaccat ccaccccgca taagtttttt aatcggtggt gtcgtcaatc actaaatcgg
     2041 aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg gagatggcat
     2101 catg
//
