LOCUS       NZ_CP115388             6072 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET20-PYO2 plasmid pCUVET20-PYO2.2,
            complete sequence.
ACCESSION   NZ_CP115388
VERSION     NZ_CP115388.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339838
            Assembly: GCF_030996785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:10:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,009
            Genes (coding)                    :: 4,683
            CDSs (with protein)               :: 4,683
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 179 of 326
            Pseudo Genes (incomplete)         :: 166 of 326
            Pseudo Genes (internal stop)      :: 42 of 326
            Pseudo Genes (multiple problems)  :: 57 of 326
            Pseudo Genes (short protein)      :: 4 of 326
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115388.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET20-PYO2"
                     /isolation_source="Endometrium"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET20-PYO2.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2020-07-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            1..915
                     /locus_tag="PCS90_RS25600"
                     /old_locus_tag="PCS90_25540"
     CDS             1..915
                     /locus_tag="PCS90_RS25600"
                     /old_locus_tag="PCS90_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032333168.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1119..1517)
                     /locus_tag="PCS90_RS25605"
                     /old_locus_tag="PCS90_25545"
     CDS             complement(1119..1517)
                     /locus_tag="PCS90_RS25605"
                     /old_locus_tag="PCS90_25545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001560793.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1694..2618
                     /locus_tag="PCS90_RS25610"
                     /old_locus_tag="PCS90_25550"
                     /pseudo=""
     CDS             1694..2618
                     /locus_tag="PCS90_RS25610"
                     /old_locus_tag="PCS90_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            2621..3154
                     /locus_tag="PCS90_RS25615"
                     /old_locus_tag="PCS90_25555"
     CDS             2621..3154
                     /locus_tag="PCS90_RS25615"
                     /old_locus_tag="PCS90_25555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031944069.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3375..5104
                     /locus_tag="PCS90_RS25620"
                     /old_locus_tag="PCS90_25560"
                     /pseudo=""
     CDS             3375..5104
                     /locus_tag="PCS90_RS25620"
                     /old_locus_tag="PCS90_25560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5106..5369
                     /locus_tag="PCS90_RS25625"
                     /old_locus_tag="PCS90_25565"
     CDS             5106..5369
                     /locus_tag="PCS90_RS25625"
                     /old_locus_tag="PCS90_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_001560791.1"
                     /translation="MELKNSISDYTEAEFVQLLKEIEKENVAATDDVLDVLLEHFVKIT
                     EHPDGTDLIYYPSDNRDDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5431..5574
                     /locus_tag="PCS90_RS25630"
                     /old_locus_tag="PCS90_25570"
     CDS             5431..5574
                     /locus_tag="PCS90_RS25630"
                     /old_locus_tag="PCS90_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001560790.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6072
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..776
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.139"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1695..1999
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="96.0"
                     /evalue="9.75e-111"
     Typing          2005..2615
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.039"
                     /coverage="99.0"
                     /evalue="8.69e-167"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt ttggtcttcg catctccggc aaagggcgtg cgcttcttgc gcggtacata
      121 cagcagaacc agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggca
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg
      241 aacggtcatg ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc
      301 tcggttaagg cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg actgatttgt aaaaatccgt tccacctgga atggcaggtg
      421 atggagtggc gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagcgc gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggcctga attctcacag
      601 tggcttgatg ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt
      661 tcaccgcctg aatgtcgggc tattggcagg agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca caccagaaat tcaggctgca
      781 cgcggtcgca agggcggttc taagtctaag cgcagcacag tggctacatc tgcacgcacg
      841 ctgaaaccgt gggaggcatt agggatcagt cgcgcctggt attaccaact gaaaaaacga
      901 ggtctcgtag agtagaccaa ataagcctat atcagataac agcgcctttt tggcgtcttt
      961 ttgagcagca ggttttttgc cgtttttccc cgttcagccc tgcaatggtg ctgttttccg
     1021 catgattgcg ctggtagtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc
     1081 gaggaagcac cagggaatcg gaacacggat ggaggcactt acgcgtctgg gtttggggat
     1141 gtctccagat cgtacttaag ttccttaagt gcctgtaata tcctgtcttt tttatagcat
     1201 agctcgcctg tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag
     1261 agccggtgat tgcgttcccg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt
     1321 tttcgttcgc tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggagg
     1381 agtttgcgtt cctgttctgt gagtcctgct tcgttgtcga gcaggtcaat aatctgtctc
     1441 tgtcgtgcgg tctgttctcc gagcttcctg atgaatccga tccgtttctt cagccattcg
     1501 atgttgctga tgcgcatgtt cgtggtgctc cctggtgttt tttgcggtgt gttgtggtcg
     1561 tgcagtatat cgcttgcggt cggtgggtgc aggggggtat cttccgtgct aattcgtaaa
     1621 attagaagga agcgcactta tatgtttctc tccttcgtcg agaaacagtg cgctcctgcg
     1681 gagggctgaa accgtggcaa cctatcacct cagcgtcaaa tttggaggca agggacaggc
     1741 tgcaaaccac gccgattaca tcgagcgcaa aggggagtat gcgaaacgga aagatctgga
     1801 atacacggag cacggaaaca tgccggaatg ggccagggat aatccttccc atttctggca
     1861 ggcggctgac cagtttgaac gtgcgaacgg ttcgacttat cgggaactgg aaatcgcgct
     1921 gccccgtgaa ctgacaccgg aacagcgcct tgaactggtg caggattttg tacgtcagga
     1981 agccggcgaa cggcacgccg gtttttgcca ttcacaatcc gaaagcgagc attgacggcg
     2041 gcgaacagcc tcatgcgcat atcatgatga gccagcgtgt gaatgatggc attgaccgta
     2101 caccggaaca gtatttccgg cgctataacg ccaggtatcc cgagcgggga ggtgcgaaaa
     2161 aggattccgg aagcctgact ctcactcagc aaaaagagca gttgcgggaa ttgcgaaaac
     2221 gctgggaggt gaagcacaac gaacatatga gaaagcatgg ctttgaaagg ggcttcattg
     2281 actgccggac gctgaaagaa cagggcattg aacgacgtcc cgaagtgcat ctgggttttg
     2341 aagctgccgg acggcttgat gatgcccagc gtcatgacat catgcaaaaa cggtctgaac
     2401 cggactattc ccgccatctt gaacaggcgc gaacggtcgg gcatgacatc agggaatggc
     2461 tggacactcc ggttaagcaa catgaactgg tcagaaggct tcaacatgat gttgcgcggg
     2521 agcatggctt tgacatgaga gacagagtga gcagacagga ccgaactgaa cctgaacggg
     2581 ttaaatcacc gtcacgtgga ttttctatgg gattctgaac atggccagca cagaagaaaa
     2641 attactcacg ctcctgttat cggtggagca tctgcaacat accgcaatgg agcaacagaa
     2701 agctgttgct gattctctgg ctgtggcaag ggaaacactt cgcggatatc gggatatttc
     2761 tgacggtgtg gcaaaacagg tcagggatga agtcagaaac gaagtgcaga ggctggatgt
     2821 tgcagggctg ataggtgagc gcatcagtca ggattttcag cggctggaag agtcagtcgc
     2881 aaacatgaag gcaaacgcgg atgccctgaa cagggatatt gcgacaattc acagcaatat
     2941 cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa tggctgggtg gggtgtcagt
     3001 ggcctttctg gttatgttca gtgtgctcgg atgggggatg aaaacaattc tcgacaggaa
     3061 ctacaacgcc acagcggcgg tgtatcagaa agttgtgaca ctggaagata aactggcgga
     3121 gaaacctgtc acgggtaaga gccggaaaaa ctgacgcgct tcgtttgtcc aacgcctcac
     3181 ggaccgcgaa aggatttcgc gaaccgttca ccgtttccat gagtgactcc tccgaactgc
     3241 caaaaaatat ccctccctga cttgacagag aaaataatgg cgagtacttt ttgatctgta
     3301 cataaaacca gtggttttat gtacagtatt aactatgtaa ttaattgttt taacgcttaa
     3361 aagagggaat ttttatgagc ggtggagatg gacgtggcca caacagtggc gcacataaca
     3421 caggtggtaa cattaatggc ggccctacgg ggcttggtgg aaatggtggg gcttctgacg
     3481 gctccggatg gagttcggaa ataacccatg gggtggcggt tccggtagtg gtgttcactg
     3541 gggaggtggc tccggccatg gcaatggcgg ggggaatagt aattccggtg gtggcagcaa
     3601 ttcatccgta gcagcaccta tggcatttgg ttttcctgct ttggctgctc ctggtgccgg
     3661 aactcttggt atttccgtat ctggtgaggc tttatctgcg gcaatagcgg atatctttgc
     3721 agccctgaaa ggtccgttta aattcagcgc atggggtatt gcgctttacg gcattctgcc
     3781 atctgaaata gcaaaagatg acccgaatat gatgtcaaag attgtgacgt cattaccggc
     3841 agaaacagtg acgaatgttc aggtcagtac acttccgctg gaccaggcaa ctgtcagcgt
     3901 gacgaaacgt gtgacagatg ttgtgaagga cacacgacag catattgccg tagttgcagg
     3961 tgtgcctatg agtgtgcctg ttgtgaatgc caaaccaaca cgtactccag gtgtatttca
     4021 cgcatcattt cctggtgtcc cttctctgac agtcagcact gtcaaaggcc ttccggtgtc
     4081 aacaaccctt ccccgtggta ttacggagga taaaggccgg actgccgttc ctgcgggatt
     4141 tacctttggt ggtggttcac atgaagcggt gattcgcttt ccgaaagaaa gtgggcagaa
     4201 gccggtttat gtatcagtga cagatgttct taccccagca caggtaaaac aacgtcagga
     4261 tgaggaaaaa cgcctccagc aggaatggaa tgacgcacat ccggtggaag tggctgaacg
     4321 caattatgaa caggcccgcg cagaactgaa tcaggctaat aaagatgttg ccagaaacca
     4381 ggaacgacag gctaaagctg ttcaggttta taattctcgt aaaagtgaac ttgatgcagc
     4441 gaataaaact cttgctgatg caaaggctga aataaaacaa ttcgagcgat ttgcccgaga
     4501 accaatggct gctggtcaca gaatgtggca aatggcaggg cttaaggccc agcgggcaca
     4561 gacggatgta aataataaga aggctgcatt tgatgctgct gcaaaagaaa agtcagatgc
     4621 tgatgttgca ttgagttctg cgttggagcg ccgcaaacag aaagaaaata aagaaaagga
     4681 cgctaaagcc aaattagata aggagagtaa acggaataag ccagggaagg caacaggtaa
     4741 aggaaaacct gtcaataata agtggttaaa taatgcaggt aaagacttag gttctcctgt
     4801 tccagatcgt atagctaata aactacgtga taaggagttt aaaagtttcg atgattttcg
     4861 taagaaattc tgggaagaag tgtcaaaaga tcctgagtta agtaaacaat ttagtaggaa
     4921 caataatgat cgaatgaagg ttggaaaagc gcccaagact agaacccagg atgtttcagg
     4981 gaagagaact tcattcgagc ttcatcatga gaagccgatc agccaaaatg gtggtgtcta
     5041 tgatatggat aacatcagcg tggtaacacc taaaaggcat attgatattc accgaggtaa
     5101 ataatatgga actgaaaaat agtattagtg attacacaga ggctgagttt gttcaacttc
     5161 ttaaggaaat tgaaaaagag aatgttgctg caactgatga tgtgttagat gtgttactcg
     5221 aacactttgt aaaaattact gagcatccag atggaacgga tctgatttat tatcctagtg
     5281 ataatagaga cgatagcccc gaagggattg tcaaggaaat taaagaatgg cgagctgcta
     5341 acggtaagcc aggatttaaa cagggctaaa atatgaatgc cggttgttta aggatgaatg
     5401 actggcattc tttcacaaca aggagtcgtt atgaaaaaaa taacagggat tattttattg
     5461 cttcttgcag ccattattct tgctgcatgt caggcaaact atatccgtga tgttcagggc
     5521 gggacagtat caccgtcgtc aactgctgaa ctgaccggag tggaaacgca gtaacctgca
     5581 atcctcattt gacaagaaca aagcgtgtca ggctgattct gacgcgcttt ttttgttgaa
     5641 gtgccacaaa aattccatgt gggagatggg atctaaaatc tccgtgcaga accttccatc
     5701 aggagggaga aaacttttcg ttttgagccg ttcggtgttc agaacgcacg aaaccgatcg
     5761 cacatcgctt tcgtgcatag ttatgcaggc ccctaaaaac gattctgacg catttttctg
     5821 gttcagcctg gtgtttcctt gtctttttgc gttttttgcg tcagaaagcg attgagcgcg
     5881 ttttagcggt gcgtttaacg ggagttatgg taaatggatc gggttttcgg gaaggttcga
     5941 caggatttgc cgttgggtgt agtgtaagcg actgaaaaac aaacgcccct gaaatcatgt
     6001 ccagatccgg caagattcaa catgaaatac agagggcgtg taggtcgcat aacccggaga
     6061 gaatcgtaac ac
//
