LOCUS       NZ_CP115380             4082 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET20-PYO4 plasmid pCUVET20-PYO4.4,
            complete sequence.
ACCESSION   NZ_CP115380
VERSION     NZ_CP115380.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339840
            Assembly: GCF_030996645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:23:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,454
            CDSs (total)                      :: 5,329
            Genes (coding)                    :: 5,010
            CDSs (with protein)               :: 5,010
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 319
            CDSs (without protein)            :: 319
            Pseudo Genes (ambiguous residues) :: 0 of 319
            Pseudo Genes (frameshifted)       :: 156 of 319
            Pseudo Genes (incomplete)         :: 183 of 319
            Pseudo Genes (internal stop)      :: 49 of 319
            Pseudo Genes (multiple problems)  :: 62 of 319
            Pseudo Genes (short protein)      :: 5 of 319
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115380.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET20-PYO4"
                     /isolation_source="Endometrium"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET20-PYO4.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2020-08-13"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(173..2332)
                     /gene="mobQ"
                     /locus_tag="PCS82_RS27260"
                     /old_locus_tag="PCS82_27190"
     CDS             complement(173..2332)
                     /gene="mobQ"
                     /locus_tag="PCS82_RS27260"
                     /old_locus_tag="PCS82_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2511..2765
                     /gene="mobC"
                     /locus_tag="PCS82_RS27265"
                     /old_locus_tag="PCS82_27195"
     CDS             2511..2765
                     /gene="mobC"
                     /locus_tag="PCS82_RS27265"
                     /old_locus_tag="PCS82_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            3298..3999
                     /locus_tag="PCS82_RS27270"
                     /old_locus_tag="PCS82_27200"
     CDS             3298..3999
                     /locus_tag="PCS82_RS27270"
                     /old_locus_tag="PCS82_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1975..2333)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2334..2510
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3299..3999
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.858"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(177..2419)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtcaaaag cgctctaatg cagcgaaaaa agaaccccca ttttttgggg gcggaaaaac
       61 aaaactatgc ttgcattatg tgtgtgcttc ttgcattcat ccctgtcgat gaacatcgaa
      121 gaacatcgac gaacataaca cgatgttaaa caaaacaaaa tattgttcac attcactgtg
      181 accaaccgcg atcatgtctc ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc
      241 attcgtccca ggtacgatta aaacccagcg agcggaacag atgatccggc tgtttttctg
      301 gtgtgttgac ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac tccataagtt
      361 ccggcgcagg tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca taccctgcat
      421 accgtaaccc cttatcggtc agtgcactga tgaccagctg gctgttatcg gctgagggaa
      481 cggcaaacgc gggatacagc gtaccattaa gccggacaac accgcccaga gagtcatcca
      541 cgcccatgac cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa ctcattaccg
      601 gcatcagcgt cggtagcaga cgtccctgcg taaaatccac cttccttgct gccagtgatg
      661 gcatggctgg ctggcggtct tctccaagac agaaagactc cggccatgtc ttcggctggc
      721 ggagattgcg ggtatattgc ggccatgtct ggcaaacctg cgtcagaagt tgctcaaagg
      781 tcgcagagga ataaacacgt tgcagtggtg gatgttgacc gatttcgctt cggtcacaga
      841 tataaccact cctgtcacgc attaaatcaa cacgctcaaa gattctggtg cggtatttct
      901 ccagctcgtt cccccagagc gtgtattctc cgttccggtt gtgaagcaga aggtaacagg
      961 tcatttcccc gttaccaggc atgggggcct cgccataccc cgtaacgact gcgctcagta
     1021 cccctgaatc cataacggca ttgtcatttt tcctgagctg ttccgctcgt cgctccgcgc
     1081 gactacgttc tgtatcctcc tgctccttcc ggcgcaattc ctcttcacgt ttaagcaact
     1141 ggactctgag agctgcaagg ccggatgccc cccactgctg ccactgtttt tcaaatgccg
     1201 tctgactcgc gggaatgtcc tgtttctcca ccttccggta atgctgtcgc aatttttcca
     1261 tttgctggct cagcgactgg atttttccct gttcgctacg gtattccggt gccgcccatt
     1321 gcatcagggg aatacgggaa aaaccggacg aaaacggcga tgattttttc aggctggcag
     1381 catggctcat cgcttccttt cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct
     1441 gcatatctgc catcatcgac atggcattac tggtgagttt ccatgccgca ttactgtcct
     1501 tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca ggaagcacga
     1561 tattcagaag gtcagccaca cggcgggcag gctctgtcag cccgctgttt ctgagttcgg
     1621 tacgggaaat ctgtatttcc aggttaagtc tgttaatcag acgattctgt tcgccgcgtt
     1681 ctgtttcgat accgcgtgtt tccatcgcac aggcagactt tccgaggtga ataccaggcg
     1741 ttttctcaag cccctgtcgc tcgtatgaac gatggtctat ctcttcctga taaccggcgt
     1801 tcgccagtgc cctgttcgca tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc
     1861 ggtcgttcca gtccctgacc tttccaccga atccagcagg tccgacagct ctcgtggtca
     1921 gcatgatgtg cgcatgggga tttgttttat ccatgtgatg gaacgccaca tcagcaatca
     1981 tgccctgact gacaaagttt tctctgacaa aggcgagaac ggtttcccgt gcggcatcct
     2041 gcggtagttc gcgggggatg gcgagttcaa tttcccgcgc aagttgtgag ttcttccggc
     2101 gttcggcctt ctcaacggca ttccagagtt cggcacggtt cgtacaccag gcaggggctg
     2161 tcgcgggagt cagaatcaca gcttcagcca caccgctttt gcgggtgtag tcgtgagtca
     2221 gtccggtacg ctcatcaagc atcagtgaac cggaacgata ggcagcagaa gcaacggcgc
     2281 tgtagccgct gtttctggag atgattttca tgctgagatg ataaatcgcc atttctgccg
     2341 taatgccttt ttgtggtttt ctttcagccc cgcagggcgc acacaatcga aacggagtgg
     2401 agattgtata agtgcgcact tcgtgatttt ccgtgcatta tgccagctca gtttttaaac
     2461 taccagtgac gcgctaaaat gcgatttttc atcgcgcacg gagttacatc atgagcagaa
     2521 cactcgaaca gaagattgct gatgctgagg ccagattaca gcgcctgaag gcgaagagca
     2581 ggagtctgga cacagcgcag aaggtcattg tgggtgcagc attgctggca aaggtcagaa
     2641 agccggagga ggtgcagttg cgtgcctggt tactccagtt cctgaaggca gaggtgacac
     2701 gacaggctga tgtgacgcgc atactaccgc tgattaacga gctggaagcg ctgccaggac
     2761 agtgattgta tcgtccgctc gccgcagtcc cagcgaccga gcgttagcga gcgagtgggc
     2821 gaggaagcgg aagtgaatcc gtcgcctgat tgagcacata cgcacggttt ttcgggaggt
     2881 gatatgcgct gaacccgcat gaatactgga tttccacagg gttggagggg gtgtattcgg
     2941 aacttgcaat tctgcttttt tgcaacgcat tgtttttatt gattttttgt tttcagcact
     3001 ccaagttatc cacaggctcg tcgttaagtt tttagatctt gtttttaaga tcttgttttt
     3061 aatacttgtt ttagcgccaa aattccttat agaaaacaaa atgttacaac gctataggta
     3121 atatgattta aggtaaaggt aatatgattt aaggtgttta gggggaggaa tttaaggtaa
     3181 aggcaatatg atttaaggta atattcacag aaaaagggat attaaaataa aaaggcattg
     3241 cctttcgttg agggtatgtt tatcttttat tcattacctt ttggggaggg atgctctatg
     3301 tcggatctaa ttgcctataa atccaatgcg ctggttgaag ccagttataa attgacccta
     3361 caggaacagc gttttttgtt actttgcatc ggtcgattga agtctggggc tgatgcagaa
     3421 tctccaaaat tgcaaaagac aatgaccatt acagctgcgg aatatttcga ttcattccct
     3481 gacatggggc ggaaaaatgc cgaggttcag ttacaggaag caattgatcg tttgtgggac
     3541 aggtcaatca ttctcaagga tgacgagaaa cgtgaggagt tccgctggat ccagtatcgg
     3601 gcacagtacg ctaaaggcga ggccagagca caaataacat tttctgacgc agtaatgcct
     3661 tatttgacac aactacaagg gcaatttact agagttgtaa tcaagaatat atctagatta
     3721 tcaagaagtt acagtataag gatatacgaa ctcctccagc agttccgcag tactggcgaa
     3781 cgtattatcg ctctcgatga ttttcgctca atgcttgata tagagcataa atatcaaaca
     3841 tataaatcac ttaaccagca attattaaga ccctgcattg acgaactgaa caaaaaaagc
     3901 gacctggctg taacggttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt
     3961 cgcttcaagg aagacaaaca aatcaagatg acgatctaaa atcgatttta aggcacctaa
     4021 aacgctccct gccgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa
     4081 tt
//
