LOCUS       NZ_CP115374             3133 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET17-972 plasmid pCUVET17-972.4, complete
            sequence.
ACCESSION   NZ_CP115374
VERSION     NZ_CP115374.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339841
            Assembly: GCF_030996545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,606
            CDSs (with protein)               :: 4,606
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 103 of 274
            Pseudo Genes (incomplete)         :: 178 of 274
            Pseudo Genes (internal stop)      :: 55 of 274
            Pseudo Genes (multiple problems)  :: 59 of 274
            Pseudo Genes (short protein)      :: 3 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115374.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET17-972"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET17-972.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-07-11"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(288..482)
                     /locus_tag="PCS79_RS24985"
                     /old_locus_tag="PCS79_24935"
     CDS             complement(288..482)
                     /locus_tag="PCS79_RS24985"
                     /old_locus_tag="PCS79_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_025492124.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFESESGTGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            767..1090
                     /gene="mobA"
                     /locus_tag="PCS79_RS24990"
                     /old_locus_tag="PCS79_24940"
     CDS             767..1090
                     /gene="mobA"
                     /locus_tag="PCS79_RS24990"
                     /old_locus_tag="PCS79_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1549..2301
                     /locus_tag="PCS79_RS24995"
                     /old_locus_tag="PCS79_24945"
     CDS             1549..2301
                     /locus_tag="PCS79_RS24995"
                     /old_locus_tag="PCS79_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249639.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKANHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(2326..2469)
                     /locus_tag="PCS79_RS25000"
                     /old_locus_tag="PCS79_24950"
     CDS             complement(2326..2469)
                     /locus_tag="PCS79_RS25000"
                     /old_locus_tag="PCS79_24950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152918943.1"
                     /translation="MNTKVLFVKKALKSITCAILSLLPALSRAVTELRSLSAARLKAGK
                     SF"
                     /name="hypothetical protein"
     gene            complement(2831..2944)
                     /locus_tag="PCS79_RS25005"
     CDS             complement(2831..2944)
                     /locus_tag="PCS79_RS25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071790583.1"
                     /translation="MHEPPVQSDYCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     Typing          521..758
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.75"
                     /coverage="99.0"
                     /evalue="1e-116"
     Typing          complement(2674..3123)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.494"
                     /coverage="90.0"
                     /evalue="1.02e-154"
     Typing          768..1087
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.32e-62"
ORIGIN
        1 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccaccggcgc
       61 ggcctttttc gggttcttgt tttttgctca catgtttctt ttggcgttat cccctgattc
      121 tgtggataac ctcattaccg tctttgagtg agctgatacc gctcgccgca gccgaacgac
      181 cgagcgtagc gagttagtga gcgaggaagc ggaagagcgc cttatgtggt attttctcct
      241 tacgcaccgg tatatcgttc tgcatcctgt ctggtgtagg aacactgtta ttccccggtg
      301 ccactttccg attcaaagcg tgtctgtatg ctgttctgga gttcttctgc gagttcgtgc
      361 agtttctcac acatggcggc ctgtgcatca gcatcgagtg agtccagttt ttcgagcagc
      421 gtcaggctct ggctttttat gaattttgcc atgttaagtg cggtttgctg ctgcttgttc
      481 atctttctgt tttctccgtt ctgtctgtca tctgcgtcgt gtgattatat cgcgtaccac
      541 ttttcgacag ttttgccgcc ggtattctgt cgtctgactc tttgacgggc atttctgcct
      601 gacaatactg tcactgccaa aaaactgccg tgcctttgtc ggtaattcga acttgctgac
      661 aggacaggat gtgtaattgt tataccgcgc atacatgcgc gctattacaa ttaccctggt
      721 cagggcttcg ccccgacacc ccatgtcaga tacggagcca tgttttatga caaaacgaag
      781 tggaagtaat acgcgcaggc gggctatcag tcgccctgtt cgtctgacgg cagaagaaga
      841 ccaggaaatc agaaaaaggg ctgctgaatg cggcaagacc gtttccggtt ttttacgggc
      901 ggcagctctc ggtaagaaag ttaactcact gactgatgat cgggtactga aagaagttat
      961 gagactgggg gcgttacaga aaaaactctt tatcgacggc aagcgtgtcg gggacagaga
     1021 gtatgcggag gtgctgatcg ctattacgga gtatcaccgg gccctgttat ccaggcttat
     1081 ggcagattag cttcccggag agaaactgtc gaaaacagac ggtatgaacg ccgtaaggcc
     1141 ccaaaccgat cgccattcac tttcatgcat agctatgcag tgagctgaaa acggtcctga
     1201 cgcatttttc cggttttcac tgggtaaaac atctcttttt gcggtgtttg cggcagaatc
     1261 gcgttcagcg cgttttagcg gtgcgcgtaa ggggagttat ggtaaattgg cagcaaagta
     1321 ttttctgatg ttgtggattg tgaagagtat tcaattctta ttatgaaaca ataactataa
     1381 ctggttgttt catatagata aatataacaa aaaagcccac tctgtagtgg gcttttttgt
     1441 tatattttac gctctagagg tacacagaaa aacggtagtt cgcctgctcc tcgaatgttg
     1501 taatggtagt ctgaaaaata ttcatagtaa agagataagt gagttttcat ggatacaaca
     1561 agtttaatta gctcaatttc tgtaccaaga ttatctttct atgaaacatt tcttgggtgc
     1621 gctactgatc aagaaaaggt tggggcatac gttgcctacc aagatctgtc tggcgatttt
     1681 ttttgtcttg tccagatgat tgaggttgct ctgcgtaatg cgataaacaa tacaatcaaa
     1741 gctaatcatg ggcctgcttg gtacgactct atacctcaaa caaagaaatc tcaggatctt
     1801 gttttaaatg cgaaacaaaa agcgctagat gagtgtggcg taaggtatac ggatgatgat
     1861 gttatttgcc gtctaacctt tggcttttgg gtttatatgt tagatgcccc atatagggat
     1921 acgcaaaggc cgtgctacat ctggactcca gagaacaagg caaaaacatt tcctcatgcg
     1981 aagaatcctt tgggtggggg ggatatgaag gttaaagcgt tgtttgacga gtttcataaa
     2041 gttcttttgt ttaggaatcg actttttcac catgagccta tttggaaaaa acaccactgc
     2101 aagtcgcata gtcaagccat aaataatatg ttgaaagagt tcaatttttt aatgaatgcc
     2161 ttgagtatcg tctctaacga gaaaaaggaa cttattgagt ttattgggca cgataggcgc
     2221 ttttatgaac gctgcacgat agagtatgtt atggggatta tagggaggat taagtgcagg
     2281 gagcttaagg tagccggttg acttttaagg aaaaatgtga tacaatcaaa agctcttacc
     2341 ggctttaagt cgcgcagccg aaaggctacg taactctgtt actgcgcgac ttaaagccgg
     2401 taagagcgaa agaattgcgc aagtaattga tttcaaggct tttttcacaa aaagaacttt
     2461 agtattcatt ttcataatcc tctatttctt atgccgttct aatggcagcg ttgacttttt
     2521 attccatcta tttgaatggt taattatacc atatctgaca tcaaatctta aggataaata
     2581 gctgattcac atcagttttt ccattaatcc acaaactttt ataatttaat tgcggatttc
     2641 cttgagttca tatcgcgccc atcgtaatct taaaaacgaa aaaaccaccc ggcaaggtgg
     2701 tttttcgaag gttcagcaac cggcaagttt ctgaacctcg gtaactggct taagaggagc
     2761 gcagtcacca aaacttgttc ttccagttta gccttaactg gcacgtaact tcaagactaa
     2821 ctcctctaaa tcagttacca gtggctgctg ccaatggcga taagtcgtgc ctttccgggt
     2881 tggactcaag acgatagtta ccggataagg cgcagtagtc ggactgaacg gggggttcgt
     2941 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     3001 gataacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     3061 gcacgaggga gccatcaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3121 acctctgact tga
//
