LOCUS       NZ_CP115373             6648 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET17-972 plasmid pCUVET17-972.3, complete
            sequence.
ACCESSION   NZ_CP115373
VERSION     NZ_CP115373.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339841
            Assembly: GCF_030996545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,606
            CDSs (with protein)               :: 4,606
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 103 of 274
            Pseudo Genes (incomplete)         :: 178 of 274
            Pseudo Genes (internal stop)      :: 55 of 274
            Pseudo Genes (multiple problems)  :: 59 of 274
            Pseudo Genes (short protein)      :: 3 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET17-972"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET17-972.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-07-11"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(join(6361..6648,1..36))
                     /locus_tag="PCS79_RS24935"
                     /old_locus_tag="PCS79_24890"
     CDS             complement(join(6361..6648,1..36))
                     /locus_tag="PCS79_RS24935"
                     /old_locus_tag="PCS79_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            100..291
                     /locus_tag="PCS79_RS24940"
                     /old_locus_tag="PCS79_24895"
     CDS             100..291
                     /locus_tag="PCS79_RS24940"
                     /old_locus_tag="PCS79_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            961..1119
                     /locus_tag="PCS79_RS24945"
     CDS             961..1119
                     /locus_tag="PCS79_RS24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            1617..1844
                     /locus_tag="PCS79_RS24950"
                     /old_locus_tag="PCS79_24900"
     CDS             1617..1844
                     /locus_tag="PCS79_RS24950"
                     /old_locus_tag="PCS79_24900"
                     /inference="COORDINATES: protein motif:HMM:NF015489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1841..3409)
                     /gene="cea"
                     /locus_tag="PCS79_RS24955"
                     /old_locus_tag="PCS79_24905"
     CDS             complement(1841..3409)
                     /gene="cea"
                     /locus_tag="PCS79_RS24955"
                     /old_locus_tag="PCS79_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            3670..4107
                     /locus_tag="PCS79_RS24960"
                     /old_locus_tag="PCS79_24910"
     CDS             3670..4107
                     /locus_tag="PCS79_RS24960"
                     /old_locus_tag="PCS79_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4188..4607
                     /locus_tag="PCS79_RS24965"
                     /old_locus_tag="PCS79_24915"
     CDS             4188..4607
                     /locus_tag="PCS79_RS24965"
                     /old_locus_tag="PCS79_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4925..5158)
                     /locus_tag="PCS79_RS24970"
                     /old_locus_tag="PCS79_24920"
     CDS             complement(4925..5158)
                     /locus_tag="PCS79_RS24970"
                     /old_locus_tag="PCS79_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5165..5683)
                     /locus_tag="PCS79_RS24975"
                     /old_locus_tag="PCS79_24925"
     CDS             complement(5165..5683)
                     /locus_tag="PCS79_RS24975"
                     /old_locus_tag="PCS79_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5721..6419)
                     /locus_tag="PCS79_RS24980"
                     /pseudo=""
     CDS             complement(<5721..6419)
                     /locus_tag="PCS79_RS24980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(377..464)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          475..1033
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4822..6371)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcatgttca tcatcagtaa cccgtattgt gagcatcctc tcgcgtttca tcggtatcat
       61 taccccatga acagaaatcc cccttacacg gaggcatcag tgactaaaca ggaaaaaacc
      121 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgctgctgga gaagctcaac
      181 gaactggacg cagatgaaca ggccgatatt tgtgaatcgc ttcacgacca cgccgatgag
      241 ctttaccgca gctgcctcgc acgtttcggg gatgacggtg aaaacctctg atacatgcag
      301 ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagctgaca agcccgtcag
      361 ggcgcgtcag caggttttag cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat
      421 agcggagtgt atactggctt aaccatgcgg catcagtgcg gattgtatga aaagtacgcc
      481 atgccgggtg tgaaatgccg cacagatgcg taaggagaaa atgcacgtcc aggcgctttt
      541 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggtactga
      601 ctcacacaaa aacggtaaca cagttatcca cagaatcagg ggataaggcc ggaaagaaca
      661 tgtgagcaaa agaccaggaa caggaagaag gccacgtagc aggcgttttt ccataggctc
      721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      901 catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1021 tccaacccgg taaggcacgc cttaacgcca ctggcagcag ccactggtaa ccggattagc
     1081 agagcgatga tggcacaaac ggtgctacag agttcttgaa gtagtggccc gactacggct
     1141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgttgg tagcggtggt ttttttgttt
     1261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1321 ctgaaccgcg atccccgtca gtttagaaga ggaggatggt gcgatggtcc ctccctgaac
     1381 atcacgtata tagttagcct gacatccaac aaggaggttt atcgcgaata ttcccacaaa
     1441 aaatcttttc ctcataactc gatccttata aaatgaaaag aatatatggc gaggtttaat
     1501 ttatgagctt aagatactac ataaaaaata ttttatttgg cctgtactgc acacttatat
     1561 atatatacct tataacaaaa aacagcgaag ggtattattt ccttgtgtca gataagatgc
     1621 tatatgcaat agtgataagc actattctat gtccatattc aaaatatgct attgaacaca
     1681 tagcttttaa cttcataaag aaagattttt tcgaaagaag aaaaaaccta aataacgccc
     1741 ccgtagcaaa attaaaccta tttatgctat ataatctact ttgtttggtc ctagcaatcc
     1801 catttggatt gctaggactt tttatatcaa taaagaataa ttaaatccct aacacctcat
     1861 ttatagtatt aagtttattc ttatcaatat aggagcatag aattcctgta acaatagcaa
     1921 taccccaaat acctaatgta gttccagcaa gcaagctaaa aagtaaagca acaacataac
     1981 tcacccctgc atctgcagct ttcttctcta atgtaagaaa tagtggcttc cagtcacctg
     2041 tatccttaat ttttaggata tcagatacca catcatatcc aaaagaaaca tgccccgtaa
     2101 tcttcaagta cttggcaaac tgatctaaat gtttagccca gtcatcatac ttcaccgatg
     2161 ccaacgcatt aaaaatagca tcacgatcgg ctttgctgaa tttcttattt aaaacatcct
     2221 tgtatttttc aaaagcagcg agagcttcat tcacattgcc gattttctta cctttagact
     2281 tatcagcaag ttcctgtgcc attttcgaat atttttcacc atatttttca gtcagcgttt
     2341 gataaaagct aactgttgca tcaacagcat ccttaatctg tgaattaagg agattattct
     2401 gtgctttttt caaattttct tcagcttcat gaacacgagc gataccggca ttacgattat
     2461 tactgacctg agaaatagcc ttctggatct gagttatatc agcatttatc cggttaatac
     2521 gtgtttctga tgctgttacc tgtttttgtt tttcttctct aatcttaccg gccccaaccc
     2581 gtcgtctggt tgcttcaaaa aaaggacggt tctgaagcgg atcattggct cttggtgata
     2641 gttttttgac cagctcatcc agttctttat atttagcgga tgcctgagcc agttcatttc
     2701 gttttccagc gagcgttttc atttctgcat cacgggcatg gatactggag cttaaacgag
     2761 aattgagagt cttaatctct ccatccattt tcaccacttc agattgtgca gcagaaagtt
     2821 ttttttgggc gatctcaaca gctttagctt cttcactcaa tgcagccagt cgtttctctt
     2881 cagcttcagc cagtttcaac tggcgttctg tttcagcctt ctcccgttca atctctttac
     2941 gtcgttgttc tgcttcctga aaagcctttt ctgctgcttc cgcttcttta cgggcttttt
     3001 cttctgcttt cgcaaggcgc aaacgctctg cttccgcctg catagctgca ttattagcat
     3061 gagcaagctc tgttgctgaa ggcgtacgtg aggcattgtg acgaagagcc tcattcacga
     3121 tatccttcag gcgctgagtc agcgcatccc tgtttgcctt tgctttcgcc tgtgcttccg
     3181 ctgcagcttt tgcccgggca gcctgctctg cctgtgtttt ctttaattga gcagtagacc
     3241 atttagcagt tgcatgaata gctgcagaac tttcactttt actgcctcct tttccacctc
     3301 cgccgccaga gccactcccg tcaggagtac cattcaaaag agtaataatt acctgtccct
     3361 tatcatcata aggaacacca tctttatagt acgctaccgc ggtttccatt ataaaatcct
     3421 ctttgacttt taaaacaata agttaaaaat aaatactgta catataacca ctggttttat
     3481 atacagcata aaaagctacg ccgctgcatt ttccctgtca agactgtgga cttccatttt
     3541 tgtgaaaacg atcaaaaaaa cagtctttca caccacgcgc tattctcgcc cgatgccaca
     3601 aaaaccagca caaacattac cgttctcaga cctcattatg ttttactgaa actatgagat
     3661 gagacatcta tgggacactg tcactttatg gcatggcaca cactccggga cgcactaaaa
     3721 atgacaggca gatcgcgttc acagttttac cgtgatatgc gcgcaggcct tgtcagttac
     3781 cgtaccggca gggacggacg acgggagttt gaaaccagtg aactgatccg ggcatacggc
     3841 gaattaaagc agaatgagac accagaaagg cacagtgagg gacatgcaga aaatccacat
     3901 gatcagcaga cagaacgcat tctccgggag ctgaatgagc tgaaacaatg cctgacgctg
     3961 atgcttgagg ataaacaggc acaggatatg gatcgcagac gccaggaagc agaacgggaa
     4021 cagctacaaa atgagatagc ccagctcagg caggcactgg aactggaaaa gaaacgggga
     4081 ttctggtcca ggttgttcgg tcgctgaacg ctgtcagaga ctgatgataa aatagtcttc
     4141 ggataataac tcaccgagaa taaatacttt aaggtaggga gacactcatg agacgtaccg
     4201 gaaacaaact ttgtcttatc gccatgataa cagcaacagt agctctcaca gcctgtaccc
     4261 caaagggcag cgtggaacaa catacccggc attacgtata tgcttctgat gacggttttg
     4321 atcccaactt ttccacccaa aaagccgaca caacacgaat gatggtgcct ttttttcggc
     4381 agttctggga tatgggagct aaagataaag cgacaggaaa atcacggagt gatgtgcaac
     4441 aacgcattca gcagtttcac agccaggaat ttttaaactc actccggggc acaactcaat
     4501 ttgcgggtac tgattaccgc agcaaagacc ttaccccgaa aaaatccagg ctgctggctg
     4561 acacgatttc tgcggtttat ctcgatggct acgagggcag acagtaagtg gatttaccat
     4621 aatcccttaa ttgtacgcac cgctaaaacg cgttcagcgc gatcacggca gcagacaggt
     4681 aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc gcctgataaa ttttaaccgt
     4741 atgaatacct atgcaaccag agggtacagg ccacattacc cccacttaat ccactgaagc
     4801 tgccattttt catggtttca ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt
     4861 tccggctgac gctcccgttc cagggataac acatgttcgc gctcctgtat cagccgttcc
     4921 tctcttatct ccagttctcg ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt
     4981 gctgcacctg ctgactcaac tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg
     5041 taagcgaaaa catcttctgc aattccacga aggcgctctc ccattcgctc agccgctgca
     5101 tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg tgtttccagc tctgtcactc
     5161 tgtgtcactc cttcagatgt acccactctt tcccctgaaa gggaatcacc tccgctgatt
     5221 tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg cacaaactcc acgccccatg
     5281 tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct gatttcttcc aggttcgcct
     5341 gtatcctccc tccaagatac cagagcgtcc cgccactcgc ggtaaacagg agaaagacta
     5401 tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccctc ctgtgctgca
     5461 tcatcgcctc tttcacccct tcccggtgtt tttccagcga ttcctctgtc gaggctgtga
     5521 acagggctat agcgtctctg attttcgtct cgtttgatgt cacagcctcg cttacagatt
     5581 cgccgagcct cctgaactcg ttgttcagca ttttctctgt agattcggct ctctctttca
     5641 gctttttctc gaactccgcg cccgtctgca aaagattgct cataaaatgc tcctttcagc
     5701 ctgatattct tcccgccgtt cggatctgca atgctgatac tgcttcgcgt caccctgacc
     5761 acttccagcc ccgcctcagt gagcgcctga atcacatcct gacggccttt tatctctccg
     5821 gcatggtaaa gtgcatctat acctcgcgtg acgccctcag caagcgcctg tttcgtttca
     5881 ggcaggttat cagggagtgt cagcgtcctg cggttctccg gggcgttcgg gtcatgcagc
     5941 ccgtaatggt gattcaccag cgtctgccag gcatcaattc taggcctgtc tgcgcggtcg
     6001 tagtacggct ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc
     6061 tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga
     6121 ccgggcatca gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggcggcagc
     6181 tccttctccg cgaacgacag aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg
     6241 atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgcgccttc gcggttacgc
     6301 tccctcccca gcaggtaatc aaccggacca ctgccaccac cttttcccct ggcatgaaat
     6361 ttaactatca tcccgcgccc cctgttccct gacggccaga cgcagccggc gcagctcatc
     6421 cccgatggcc atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct
     6481 gttcacctta cgggctgtct gattcaggtt atttccgatg gcggccagct gacgcagtaa
     6541 cggcggtgcc agtgtcggca gttttccgga acgggcaacc ggctccccca ggcagacccg
     6601 ccgcatccat accgccagtt gtttaccctc acagcgttca agtaaccg
//
