LOCUS       NZ_CP115372            12602 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET17-972 plasmid pCUVET17-972.2, complete
            sequence.
ACCESSION   NZ_CP115372
VERSION     NZ_CP115372.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339841
            Assembly: GCF_030996545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:16:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,606
            CDSs (with protein)               :: 4,606
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 103 of 274
            Pseudo Genes (incomplete)         :: 178 of 274
            Pseudo Genes (internal stop)      :: 55 of 274
            Pseudo Genes (multiple problems)  :: 59 of 274
            Pseudo Genes (short protein)      :: 3 of 274
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115372.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET17-972"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET17-972.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2017-07-11"
                     /collected_by="Pattrarat Chanchaithong"
     gene            104..1648
                     /locus_tag="PCS79_RS24865"
                     /old_locus_tag="PCS79_24820"
     CDS             104..1648
                     /locus_tag="PCS79_RS24865"
                     /old_locus_tag="PCS79_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<1741..2005)
                     /locus_tag="PCS79_RS24870"
                     /old_locus_tag="PCS79_24825"
                     /pseudo=""
     CDS             complement(<1741..2005)
                     /locus_tag="PCS79_RS24870"
                     /old_locus_tag="PCS79_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(2150..3026)
                     /locus_tag="PCS79_RS24875"
                     /old_locus_tag="PCS79_24830"
                     /pseudo=""
     CDS             complement(2150..3026)
                     /locus_tag="PCS79_RS24875"
                     /old_locus_tag="PCS79_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(3225..3509)
                     /gene="nadS"
                     /locus_tag="PCS79_RS24880"
                     /old_locus_tag="PCS79_24835"
     CDS             complement(3225..3509)
                     /gene="nadS"
                     /locus_tag="PCS79_RS24880"
                     /old_locus_tag="PCS79_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(3511..3843)
                     /locus_tag="PCS79_RS24885"
                     /old_locus_tag="PCS79_24840"
     CDS             complement(3511..3843)
                     /locus_tag="PCS79_RS24885"
                     /old_locus_tag="PCS79_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3988..4224)
                     /locus_tag="PCS79_RS24890"
                     /old_locus_tag="PCS79_24845"
     CDS             complement(3988..4224)
                     /locus_tag="PCS79_RS24890"
                     /old_locus_tag="PCS79_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4569..4937
                     /locus_tag="PCS79_RS24895"
                     /old_locus_tag="PCS79_24850"
     CDS             4569..4937
                     /locus_tag="PCS79_RS24895"
                     /old_locus_tag="PCS79_24850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(5367..5501)
                     /locus_tag="PCS79_RS24900"
                     /old_locus_tag="PCS79_24855"
     CDS             complement(5367..5501)
                     /locus_tag="PCS79_RS24900"
                     /old_locus_tag="PCS79_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(6685..7002)
                     /locus_tag="PCS79_RS24905"
                     /old_locus_tag="PCS79_24860"
     CDS             complement(6685..7002)
                     /locus_tag="PCS79_RS24905"
                     /old_locus_tag="PCS79_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            8087..8236
                     /locus_tag="PCS79_RS24910"
                     /old_locus_tag="PCS79_24865"
     CDS             8087..8236
                     /locus_tag="PCS79_RS24910"
                     /old_locus_tag="PCS79_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            8696..9924
                     /locus_tag="PCS79_RS24915"
                     /old_locus_tag="PCS79_24870"
     CDS             join(8696..9011,9011..9924)
                     /locus_tag="PCS79_RS24915"
                     /old_locus_tag="PCS79_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            9955..10323
                     /locus_tag="PCS79_RS24920"
                     /old_locus_tag="PCS79_24875"
     CDS             9955..10323
                     /locus_tag="PCS79_RS24920"
                     /old_locus_tag="PCS79_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            10408..11391
                     /locus_tag="PCS79_RS24925"
                     /old_locus_tag="PCS79_24880"
     CDS             10408..11391
                     /locus_tag="PCS79_RS24925"
                     /old_locus_tag="PCS79_24880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(11850..12547)
                     /locus_tag="PCS79_RS24930"
                     /old_locus_tag="PCS79_24885"
     CDS             complement(join(11850..12298,12298..12547))
                     /locus_tag="PCS79_RS24930"
                     /old_locus_tag="PCS79_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          7879..8052
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(5394..5759)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(7404..7912)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.314"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cactattggt ggagatgatc ataatgcctt ataatcatac gggatacgaa cttataaagt
       61 cctaaagttt agctaaatcg gaatctacaa cgtagatgat agcttgaaga attatgatgt
      121 cttttatggt ggtatcttta aaaccatcac ggattataaa ttattgaaaa ttcaaaagga
      181 tattaaaaac aatatgactg cactagtcag tagttttaaa tgggttggca ttggacaact
      241 tgtaaaaata gcaacacaat tggtttcgtt attctacttg acgcacttaa tccccccatc
      301 gagctatgga ctattagcaa tgtcatctgt ggttatgaat ttagcaagca tattcaatga
      361 catgggaact ggggcggcag taattcagcg acaagaggcg tcgatcggtt tttataatta
      421 catatacagg ataaacattt ttactgggtt gtttgttatg ctgcttgtaa taataacatc
      481 cccattaatt gtcgtgtact ttaacacacc cgagctatac ccggttctat ttctattggc
      541 ttttagcttt ccaatttcca gcttaacaat aactcacaaa gcaaaattag aaaaagaaat
      601 gaactttaaa gtcatcattg caattgaaat tttctctagc cttacaggaa tggtgctggc
      661 aatagtttta gctaatttag gctttggtgt ttatagtttg atttttcaaa cactgacaac
      721 aatatcaata tccacaatat gttttttgaa ggtttccggg ttgagtttat cattgagaat
      781 taaaaatatc agccatagtg acaagtcagg agttataggg tttagtggtt atttattgtc
      841 ttttaattta ataaattatt tttccaggaa tcttgattct attttaatag gccgtttttt
      901 ctcatccgca atacttgggg tctattcgat tgcctaccgc attatgttat ttccgttgca
      961 gagcctgacg cttgtttttt ctagagtttt cttgccacac cttagcaaaa acataaataa
     1021 cactaataac actaataaac aagattactt aaatgcatta aaacttatac tatcattaag
     1081 cgcgccactg atgctaggtt tatctgctac atctttttct tttacgagga ttttctttga
     1141 tcctaagtgg cacctacttg gagacttatt gcattggttg gcacctacag cgattatcca
     1201 atcagccttg agtacaacag gttctgtttt tatggcctac ggaaaaacaa ggtggttgtt
     1261 tttcttaggt tgcattggcg cttttttgat ggggttgtct ttcattatag gtgttcattt
     1321 cgatataaaa acattggtct acctttattt ttttgcaaac ttaattaatt cactcccggc
     1381 atttatttta cttaagaata tcttgaagtt tagttacatt gaattggtaa acgtgttttt
     1441 tagaactatc tccccagctt ttttgatgtt cttacttatt aaagtatggc agcatttttc
     1501 ctttttcaaa aatgccagca ttgatttttc ttttaatgtc gcattgggta tttttagtta
     1561 tattgttttt tataccttgt ttaatttcaa tgaggttgtt aggacaggag taattcataa
     1621 gttaagatgc atgaccaagt taaagtgatt catttattaa attcattaaa tgaattggta
     1681 atgactccaa cttactgctt gtgttttatg ttcagataat gcccgatacc gacttcggcc
     1741 taattttttt aagtgtcgta aaatcgtgtt gaggacagtg cccataatgc gggatgtggc
     1801 ccggcatcca acgccattca tggccatatc aatgattttc tggtgtgtac cgggctcaga
     1861 ggcggtgtaa gtgaactgta gttgcttgct atgttttacg acagtgagag cagagatagc
     1921 gttgatgtcc ggtagtgctt ttaccgttac gcaccacgcc ttcagtagct gaacaggagg
     1981 gataactgat ggaaatggaa gccacgggag cacctcaaaa acaccattag acactaaacc
     2041 agcaagttgg catcatcaca aacagttgtt actgctggaa aaaaaacact atcaacactt
     2101 aagtaatggt gattcaataa ttaagattca gtatgtaaaa tactgaatct taattataaa
     2161 ttttcctttt tctaaaaaat gtagcgcgac taatacctaa agcaacccaa ggctccccta
     2221 gagacagcct acctcctcct gatgattttt caccaccaac ttttcctctt ttggcttgta
     2281 taagtgaaaa attttgctta ttaaatttca aaaatgtcca cttgcagata cttttcgcta
     2341 ttcccgttac ttcattctca ttcaaaggca ctgcaaattg cagattgtaa gcgcaggcgc
     2401 gttcatggca ggcttgtagc cactgttcgt actcaggcca gccctgacgg atagcgcgat
     2461 atgcccactt tctggtttta tcaaacagta tgcagttacg acctaagcca taatcggcga
     2521 cgattttttt atcgttcgca gcgttcaggt cgagtgattc ggctaaccag tccagcgtgt
     2581 agagttcagg ctgccatacg ggcaattttc cagtgactgt ggttcggatt cttgcaaatt
     2641 agccccgaat accccgtgtc agcgcccaat tttttacgta gcgcgctctc tacagcagcg
     2701 gcgtaacgta acggtttcat ttttccgtct ggcgcggtgc gtatcgaggt tttcagtgcg
     2761 tacagcaggt gcgcgtgtcc gttttcgggg ttggtgatgg tcagtgtcgg cgcgggggcg
     2821 ttgcggtcgc tccagtcaat ggctgcgccg atgcggtcaa cgtcaaatcc cagccagaac
     2881 atggcgtgag gctgattaaa ctggatgtat ttagccagaa tagcgcgctc tttccctgcg
     2941 atgcggacac caaagtgtaa atcatccgag aagtacggtt tatgcggcaa gcggtcgttg
     3001 aaaagttcga gtgctgcgtt acgcaatgtg ccctgaactt atggcgggct gttgtctggc
     3061 acggttcatg tgctaatatc cttcttaggt tttataccta gccctgattt atcatgtctc
     3121 ttccgccaag ttgaaatcat gattatcggg gctttgtttt ttctgttgcc gttaaagtta
     3181 cacgcgaacg gcctcgcctg tcgaatttct tctgtcacat cagcttaatg cgccgccagt
     3241 tcatcaaaaa actttgggtt agcctgaatg gtggccagca cttttgctgc cagtccggtt
     3301 gggttacggc gtccggtttc ccagcttttg atggtatcga ggctggttcc cagcactttc
     3361 gccatttcgc tctgagtaat attcaggttt tctctgatag ccctgacgtc tgccagctcg
     3421 tagcgggtca cacgggcggg ggctttggtt ccgtttttga tttcaacggc ctcttccaga
     3481 gaggctttca gctcgtcaaa aaaactcatc ttacacctcg tctttcagtc gttgggtcag
     3541 cttcttcagt tctgctttct ctgcgtctgt gatgctgtct ttggtgcttt tagggtaggc
     3601 catcaccaga taaatcacct ctgcggtggc aaggaagtaa atcacccgcg cactgccgct
     3661 ctttccctga ttccccgtcg ccatcctgat ctttcgcaga ccgcccgttt tctggatgag
     3721 gtcacctttg tccggtgact cgataagctc tttctgaagc tctttgatct catcatccgt
     3781 ggcgatttgc tttatctgac gggtaaacat cgttgtctcg ataaactcaa tcgcgtgact
     3841 cacggctgtc tcctgtatat tttgatgtac aatgtacacc aagaggcgtg ttctgaaaag
     3901 ccgcgttctg tgccatcaga cgttacatgg actgacctcg atagcgggtt gcttgcttct
     3961 cctgctgtaa ctgccgttcc tgctgcttta cgagcgtgtt aatcggctca gatcgctgct
     4021 caagcgttcg acctgttcgt gcaaggcgtt cacctgattg ctcaagttca gacataccct
     4081 gtcgttgcgc tccagtgcct gtgaggtagt ctcgaacatg cgttgcaagc tgctgtaact
     4141 gtcctgccac gcctgcttct gctgttcgta gcttgtttgt aagctttcta aggcgctcag
     4201 aagctgcctt tccatctcgg tcatggtttt tcctgtcctc agtgataaaa acgtgctgtg
     4261 gcgtttctgt ggtgtctgac ggggttattt cgcggtgtac ttcatcacgg tgtttccggc
     4321 tgtcgatgtg tatgtctcgg tgccatgcgc tttcttcggc agtaccacca gataggtgtt
     4381 cttctgcttg tcgtccttgt aaatcgctac ccctgcgccg ctctggggtc cgcttggaaa
     4441 aaggaaatta tcctacgcta agcgcatttt ttgcacagat ggctaccgac tgaacgtaac
     4501 gtgtgagtca tgatcagaaa tgtgttaact ctaggaattg acacgactta ctgtttgatg
     4561 gggaaatcat gtttagctat ctcaaggcta tgtatcatca atcaaaaatt caggctgaac
     4621 tcaaggcaca gatacatgaa caaactacgg ttaatgctat ctgtcatcac cctgaatcaa
     4681 tcgagatcat tgcggtttgt tcgactgatg cgtattaccg aaaacgtaag gacgctgctt
     4741 ttctgacaac ctgttctgtg ctcatgcgta ctttaaaaga tgaatccgta ccgatggtac
     4801 tacggaaaac agcatggcga ttattgaacg aaagatacca acgaattaag ttaaatcagg
     4861 cttataggat agaaaatttc ttgcttgttg ccgattttga atatgctctc gaagaacatg
     4921 atgaactagc agagtagtac tagtgaggcg atgaataccg acagttaacg tgcatgtgac
     4981 ttaaacgtcc gattacgtac agcatgtaga caacatgtcc gaaagtaagc aaaaccatta
     5041 acggacagcc ggatcatttc gtacatccct tacggtcagg ggttcgcgct caatggaacg
     5101 aaatcacccg ttagcttaaa acgcttaacg taggataatt tcctttttcc aagcggaccc
     5161 gcaatacagc aaggaacttc ttgagatcct ttctgttgcg tcgtaatctc ttgctctgta
     5221 aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta gctaatcctg gcagattttc
     5281 taaccgcggt aacagtcttg tgcgagacat gtcaccaaat ctgtcctttc agtgtagccg
     5341 cactcaggcc atcacttcaa gaactcttac tgcatctctc gcacatcctg tttaccaatg
     5401 gccgctgcca gtggcgtttt tgcgtgtctt tccggattgg actcaagacg atagttaccg
     5461 gacaagacgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     5521 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     5581 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg
     5641 aaacgcctgg gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     5701 tctgtgatgc ttgtcagggg ggcggagcct atggaaaacc ggcagcgccg cggcctttcc
     5761 tccctcgctt tatctcgtct tcatcgccat atgcctttgt gaatattctc cgtccgctcg
     5821 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atcttcggcc
     5881 tcacgtccgg caccttacac tccgtaactg cattttttca taggatgcag gatagggcta
     5941 acgccgcaaa gtgacgtcac ctgacgttcc tggattacaa aggttaaagc agcggacagg
     6001 aaatgttttg tgcccacagc tatgctgggt tattcacaaa acagcgggac aaaatgtgtt
     6061 tttggagtac cgccggcccg ctaaagactc ctggttagct cccgcatgtt tgctatcttt
     6121 ttgctaccat tttgctactt ttttgctatc tccagagtct tctggtcttt cggttgctat
     6181 ccttttgcta cttttttgct atcaaaatgc tacctctccc ttcttgcaat aaatgaccaa
     6241 ggcacgttag cgatgtatac atcgccgcgc cgcaagggtg ccccttccga atcccgttca
     6301 tcactgtgtg ctgattaatc caggttaaat ccgaccccct tagcaataaa tcctgccatg
     6361 cctgacggcc tggccaacgc ctcacggtcg cacagtaaac tgcgcaccct ttcaccgtta
     6421 tcggtggggt tcgtggtggc tttcagggcg ctcgccggat tttagccgct aaaatgacgt
     6481 ccatgcgttc gtgtgcatag ccatgcagac gcgaagaaat catcaggcgc gttttttcac
     6541 gatatggaca gggaaagatg ccggaaatag gcaggagggc gcagaaatgc cctgagggcg
     6601 tctgagatgg tgcacgcaac ggatgttatg gtaaattcag ctgattggca ggtacaaaaa
     6661 aagccccgag ggcttttttg taccttattg atctgcacac tttgcagcct tcagggcttc
     6721 cagctgattt agccggttag ctgctttttt gcgggcttcg ccttttgcca tgccatttcc
     6781 tatcccaaaa tcacccagga aacctaatac ggtgcgcccg tcaaattcgc cggtttcctc
     6841 aatttcttgt tgtacactgc gcgttttggc gatttccaga gctaccgctt tgcaatccat
     6901 tgcggaagat tctggaccgg tgacgttcac ggcctgtgga tattgctttg ttgcgcatcc
     6961 tgccatgatc agcgctgccg ataggatgaa tatttttttc aaaattatcc ttatttattt
     7021 aggcatagtt aagagatacc cattttatcg gcaggaaatg agggtttttt agttgaattt
     7081 tgggaggggc aaggttccat attggtcgag gattctggag ctgaccggca acggccagcg
     7141 cggctatggg ccatgcggtg gttgagcata gaaaggatct tcttgagttc ctttttttct
     7201 acgcgtaatc tcttgctctg aaaacgaaaa aaccacctgg ggaggtggtt tttcgaaggt
     7261 taagtcagtt ggggaactgc ttaacctggt aactggcttc agcagagcgc agataccaaa
     7321 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactcta tagcaccacc
     7381 tacattaact cgctctgcta atcctgttac cagtggctgc tgccagtggc gctttgtcgt
     7441 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcag tcggactgaa
     7501 cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcacggaa ctgagtgtca
     7561 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     7621 ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct
     7681 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     7741 agcctatgga aaaacggctt tgccgcggcc ctatcgcttc cctgttaagt atttccctgg
     7801 catcttccag gacatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga
     7861 ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct
     7921 gacgcaccgg tgccgccttt tttgtcctgc cacatgaagc acttcactga ctccctcatc
     7981 agtgccaaca tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg
     8041 cgccccgaaa ccctctaaca acctctcagc tactggatat caccccatgc catctgaaca
     8101 tgatgaacaa ctggattcga ctgatacgca gcttcccggc gaatatgacc tttttgattt
     8161 aatggcgcag gttactgaag agaacctgca tgccctggtg gattttgggc cgcctgtcgg
     8221 ccgggaagcg ttatagcttt ttcgcttacc cgcgtacgtg gtaatacgcc ggtattacat
     8281 cttatttctt tattttcatg taattacagg tggttgtaat acgttgcgta ttacataaat
     8341 aaacggggac ttcagttttt atcttttaat tcatgcaatt gcgatgaaaa tcgcggttgc
     8401 gtatggtgta tgaccattta agggataatg taacctgact tttaaagaat gaatctatct
     8461 ttggcagcgc atgagatcga taaaaagcgt tccacatcag gcggttcatg tatccatgaa
     8521 ccggaaagga caaaaataag acaattgtaa gacatttttt gcgttttact cttattttgg
     8581 actattatga gacaaatgta agactagact ggccccctga atctccagac aaccagtatc
     8641 acttaaataa gtgatagtct taatactagt ttttagacta gtcattggag aacagatgat
     8701 tgatgtctta gggccggaga aacgcagacg gcgtaccaca caggaaaaga tcgcaattgt
     8761 tcagcagagc tttgaaccgg ggatgacggt ctccctcgtt gcccggcaac atggtgtagc
     8821 agccagccag ttatttctct ggcgtaagca ataccaggaa ggaagtctta ctgctgtcgc
     8881 cgccggagaa caggttgttc ctgcctctga acttgctgcc gccatgaagc agattaaaga
     8941 actccagcgc ctgctcggca agaaaacgat ggaaaatgaa ctcctcaaag aagccgttga
     9001 atatggacgg gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg atggggagta
     9061 agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga
     9121 accgatgact ggatggatgg ccgccgcagt cgtcacactg atgatacgga tgtgcttctc
     9181 cgtatacacc atgttatcgg agagctgccc acgtatggtt atcgtcgggt atgggcgctg
     9241 cttcgcagac aggcagaact tgatggtatg cctgcgatca atgccaaacg tgtttaccgg
     9301 atcatgcgcc agaatgcgct gttgcttgag cgaaaacctg ctgtaccgcc atcgaaacgg
     9361 gcacatacag gcagagtggc cgtgaaagaa agcaatcagc gatggtgctc tgacgggttc
     9421 gagttctgct gtgataacgg agagagactg cgtgtcacgt tcgcgctgga ctgctgtgat
     9481 cgtgaggcac tgcactgggc ggtcactacc ggcggcttca acagtgaaac agtacaggac
     9541 gtcatgctgg gagcggtgga acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag
     9601 tggctgacgg ataatggttc atgctaccgg gctaatgaaa cacgccagtt cgcccggatg
     9661 ttgggacttg aaccgaagaa cacggcggtg cggagtccgg agagtaacgg aatagcagag
     9721 agcttcgtga aaacgataaa gcgtgactac atcagtatca tgcccaaacc agacgggtta
     9781 acggcagcaa agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt
     9841 gcgctgggtt atcgctcgcc acgggaatat ctgcggcagc gggcttgtaa tgggttaagt
     9901 gataacagat gtctggaaat ataggggcaa atccaaagac attatgggcg gtatatggcg
     9961 aattttaacc ctggtccaag aacagccatt gcagctgtca gggattccaa gaacgatata
    10021 gaaacctgga agaaaaatgg tattcgtagg gaagagattg tgactgtgct gaatgaacgt
    10081 tacggcctga atatctcact ggttgcgttt gataaggctc tttacagatt gagaaaaagt
    10141 acagatgaac cgtctcttgc tgatgtggat tctgtgtctg ctgcagaaac gaatttctct
    10201 gaagccagtg ggcctgacaa tggttttttt ctgtccggga atgacttcat gcgagcagaa
    10261 gaagaatctc aaagagtggt tgagtcattc agagatagcc ttagcaggaa gaaaggattc
    10321 tgatttattc aacaaagccg ttgataaatc gcattccata aataacaact tgatgttagt
    10381 ataactaaat attgtttgga gattactgtg aaaaaaaaat tttcgataat agtacctgta
    10441 tataacgttg aaaaatactt aagggaatgt attcaatcag tattagatca ggattttaac
    10501 gactatgaga taatctgtgt aaatgatggt tcaacagatg gcagtctaga tatcatactg
    10561 tcatttgatg accaaagaat caaagtaata aatcaggaaa ataaaggcct gtcggtggca
    10621 agaaatgtcg ccattgaatc atcaactggt gaatatatat ggtttttaga tagtgatgat
    10681 aaaatatcac atggaagtct aaagttaata gacaagataa ccaatgaaaa aaattatggg
    10741 attattttct ttaatacaca tgtttttttt gacactccag aactgaaaaa aaaaggctgg
    10801 catgattata agcgttcaac atttggtgaa atggatgcat taactttctt tgaaaaagaa
    10861 atgacgaaca aaaaactaat agtgtcagca tgctgctatg tttttagaag ggaattatta
    10921 aatggcaatg cattcattcc gcatatatat catgaggata atcactttat agcaaccctg
    10981 atttttgaca attattcaac tatatgcctc tcgttggatg aggcgttata tcttaggagg
    11041 gtaagaaatg attccataat gacgtctaaa aaatcaatga ggcacatcat tggatattca
    11101 acatgcatcg atgagcttac gaagtataaa aaaacaaaag gcatacaaaa caagagcttt
    11161 gatagatttg tgaatggaat ggttttgtcg ttattctcta tagcggaaac aataaaaaca
    11221 aaggaagcaa tgaactatgt ggcgagatat aaaaaacacc ccgacctatc atttaaagat
    11281 agagcattaa ttaattaccc attaattaaa aagacttatt taaaactaaa aaacgggaag
    11341 gtgtttaatt acctaaggaa tggcattatt aataaaaaaa gcaaccctta ataaaagcaa
    11401 agcgataatt aataaaggtt tttctttgtt gaataaataa gatttcggat aggtatcccc
    11461 gtgacgggtt gggttttcag gcgatacgca cgctttccgg ggctttgttg aataaatcga
    11521 acttttgctg agttgaagga tcagatcggg gtcagtttgg agatcggaaa tttttgtacg
    11581 ttaagcgcat tatttaacca atcttggttc gtaaggggtt gattttgcgt cagtggctat
    11641 cgcttttaag ttgtcgctct tgcagtatcg catttaggtt atccgattaa ggtcaaacct
    11701 ctgaaaatgc cgtatagcgc gggagtacac cctattgcta taggtaagtc tgttcaaaaa
    11761 acaggcttac cgtacaataa ttctctatat ccaaactaac cccagatcac gcatcttccc
    11821 gacaacgcag accgggtaat gactccaact tactgatagt gttttatgtt cagataatgc
    11881 ccgatgacct tgtcatgcag ctccaccgat tttgagaacg acagtgactt ccgtcccagc
    11941 cttgccagat gttgtctcag attcagatta tgtcgctcaa tgcgctgcgt atatcgcttg
    12001 ctgataacgt gcagctttcc cttcaggcgt gattcataca gcggccagcc atccgtcatc
    12061 cataccacga cctcaaaggc cgacagcagg ctcagaagac gctccagtgt ggccagagtg
    12121 cgttcaccga agacgtgcgc cacaaccgtc ctccgtatcc tgtcatacgc gtaaaacagc
    12181 cagcgctgac gtgatttagc accgacgtag ccccaatgtt cgtccatttc agcgcagaca
    12241 atcacatcac ttcccggttg tatgcgcgag gttaccgact gcggcctgag ttttttaagt
    12301 gacgtaaaac cgtgttgagg ccaacgccca taatgcgtgc actggcgcga catccgacgc
    12361 cattcatggc catatcaatg attttctggt gcgtaccggg ctgagaggcg gtgtaagtga
    12421 actgtagttg ccatgtttta cggcaatgag agcagagata gcgctgatgt ccggcagtgc
    12481 ttttgccgtt acgcaccacg ccttcagtag cggagcagga aggacatctg atggaaatgg
    12541 aagccacgca agcaccttaa aatcaccatc atacactaaa tcagtaagtt ggcagcatta
    12601 cc
//
