LOCUS       NZ_CP115369             6710 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET19-1426 plasmid pCUVET19-1426.4,
            complete sequence.
ACCESSION   NZ_CP115369
VERSION     NZ_CP115369.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339842
            Assembly: GCF_030996525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 12-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:06:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,736
            CDSs (total)                      :: 4,607
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 19
            Pseudo Genes (total)              :: 280
            CDSs (without protein)            :: 280
            Pseudo Genes (ambiguous residues) :: 0 of 280
            Pseudo Genes (frameshifted)       :: 109 of 280
            Pseudo Genes (incomplete)         :: 168 of 280
            Pseudo Genes (internal stop)      :: 40 of 280
            Pseudo Genes (multiple problems)  :: 39 of 280
            Pseudo Genes (short protein)      :: 3 of 280
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115369.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET19-1426"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET19-1426.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2019-06-20"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(383..>673)
                     /locus_tag="PCS91_RS23645"
                     /old_locus_tag="PCS91_23615"
                     /pseudo=""
     CDS             complement(383..>673)
                     /locus_tag="PCS91_RS23645"
                     /old_locus_tag="PCS91_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /name="type II restriction endonuclease"
     gene            complement(758..2329)
                     /locus_tag="PCS91_RS23650"
                     /old_locus_tag="PCS91_23620"
     CDS             complement(758..2329)
                     /locus_tag="PCS91_RS23650"
                     /old_locus_tag="PCS91_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017782005.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_039026336.1"
                     /translation="MTHIACHINTLQLPFVACTVPLTFTLPAAGCTLIGRNGIGKSLLL
                     ELLAGQRRADSGKIEWLTPISWLSQFAPAQDHDCAASVADLIGWGEPLRALERIEQGSC
                     AQDDFELLADRWNLRSDLADLLAEAGLRLDPHVPVSTLSGGQLTRLRLLALFARPAHFL
                     LLDEPDNHLDASGIDWLSQQLARHRGGYLLVSHDRRLLNQTSHILELSEQGLQSARLSF
                     DEFLAQQAEVQAARSTSLAQAVRSEKAAARSLQKVREQAARRASQGRRERGSQAKILLD
                     FKAEQAGKSLGRVLDRHERGHSAQVEARTEAAAALAAVQPLIMQWPEEIERKGAAQPLV
                     ITAASHVRWHGQRVDLVLQRGERCLLGGRNGCGKSSLLQMLRGVLEPETGSFRINGKMA
                     CLDQGLSLIARDLSPLANLLTVAPTLAESDARTRLAGIALRGDRALTPCHGLSGGERLK
                     LGLLMVLAQLPDLLLLDEPDNHLDHPSRQLLTQVLADYPGTLLLVSHDPDFVAGVGVGK
                     ELNLSE"
                     /name="ATP-binding cassette domain-containing protein"
     gene            complement(2833..2976)
                     /locus_tag="PCS91_RS23655"
                     /old_locus_tag="PCS91_23625"
     CDS             complement(2833..2976)
                     /locus_tag="PCS91_RS23655"
                     /old_locus_tag="PCS91_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_021553018.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGVE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3035..3295)
                     /locus_tag="PCS91_RS23660"
                     /old_locus_tag="PCS91_23630"
     CDS             complement(3035..3295)
                     /locus_tag="PCS91_RS23660"
                     /old_locus_tag="PCS91_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(3466..3999)
                     /locus_tag="PCS91_RS23665"
                     /old_locus_tag="PCS91_23635"
     CDS             complement(3466..3999)
                     /locus_tag="PCS91_RS23665"
                     /old_locus_tag="PCS91_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168772.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4002..4928)
                     /locus_tag="PCS91_RS23670"
                     /old_locus_tag="PCS91_23640"
     CDS             complement(4002..4928)
                     /locus_tag="PCS91_RS23670"
                     /old_locus_tag="PCS91_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_032707419.1"
                     /translation="MATYHLSVKFGSKGQAANHADYIERKGEYAKRKDLEYTEHGNMPE
                     WARDNPSHFWLAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDARRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHGVD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            5105..5503
                     /locus_tag="PCS91_RS23675"
                     /old_locus_tag="PCS91_23645"
     CDS             5105..5503
                     /locus_tag="PCS91_RS23675"
                     /old_locus_tag="PCS91_23645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021526524.1"
                     /translation="MRISNIEWLKKRLGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(5697..6632)
                     /locus_tag="PCS91_RS23680"
                     /old_locus_tag="PCS91_23650"
     CDS             complement(5697..6632)
                     /locus_tag="PCS91_RS23680"
                     /old_locus_tag="PCS91_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_309173316.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     Typing          complement(5698..6632)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.329"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4006..4928)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.481"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggggcgtttg tttttcagtc gcttacacta cccccaacaa caaaacctgt cgagccttcc
       61 cgcaaaaccg atccatttac cataactccc gttaaacgca ccgctaaaac gcgctcaatc
      121 gctttctgac gcaaaaaacg caaaaagaca aggaaacacc aggctgaacc agaaaaatgc
      181 gtcagaatcg tttttagggg cctgcataac tatgcacgaa agcgatgcgc gatcgatttc
      241 gactgttccg aagggcgaac aggtcaaaat gacaagtttt ctccctccgg atggaagttt
      301 ctgcacgggg attttagatc ccatcttcca ggggtcggct cagaaaacgg aaaaaatcgt
      361 acgctaagcc gaagttcagc actcacgcat tctgtcgagc acgtaccagt tccgtgaaac
      421 tgacaacatc catcaaccat gcctgctggg ctggcgtata ggtctggtgc agactgcgcg
      481 gcagaacgag ttgcagccgc tttgctgcca tttcgtcggt ctgatgcgtg gagattgctg
      541 tttccagcgt cagcagatgc ttgtcatcaa tgcggtctgc ttcggcgaga acctgccgcc
      601 agcggtcctt gcaggtcgat ttcacaccaa gcatggtcag cttcagcgag tcgaacgccg
      661 ggttatggta ttcggggttg taagccggaa ccgccgaaat ttccgtcaac tcctctaacc
      721 ccctgccaca agggcatcta ggtcaatcat cttgcggtca ctctgaaaga tttaattctt
      781 taccaacccc aacccctgca acgaagtcgg gatcgtgaga aaccaatagc agagtgcctg
      841 ggtagtccgc caggacctga gtgagcaact gacgcgaggg gtgatccaga tggttgtccg
      901 gttcatcgag cagcagcagg tcgggcagtt gcgccagtac catcagcaga cccagtttta
      961 acctctcccc cccactcaga ccatgacaag gcgtcagagc acgatcgccg cgcagggcta
     1021 tgcccgccag ccgtgttctc gcatcactct cggccagtgt cggagccact gtcaacaggt
     1081 tggcaagcgg gctgagatcc cgcgcaatca ggctgagccc ctgatcgagg caagccatct
     1141 tgccattgat gcgaaagctg cccgtctcgg gctccagcac cccacgcaac atctgcagca
     1201 gactcgattt gccgcagcca ttgcgaccgc cgagcaaaca gcgctcaccc cgttgcagca
     1261 ccagatcaac ccgctggcca tgccagcgca cgtgagacgc agccgtaatc accagcggct
     1321 gggcagcccc cttgcgctcg atctcctccg gccactgcat gatgagcggc tgcaccgccg
     1381 ccagcgcggc tgctgcctcg gtgcgggcct ccacttgcgc actgtggccc cgctcgtggc
     1441 ggtccagcac tctgccaagg gatttgcctg cctgttccgc cttgaaatca agcaggattt
     1501 tggcttggga gccgcgctca cgccgcccct gactggctcg tctggccgcc tgttcacgca
     1561 ctttttgcaa gctgcgcgcc gccgcctttt cgcttcgcac cgcttgcgcc aaactggtgc
     1621 tgcgggcagc ctgtacctcg gcctgctgtg ccagaaactc gtcaaacgag aggcgggcac
     1681 tctgcaaccc ctgctcgctc agctccagaa tatgactggt ttgattgagc aggcgccggt
     1741 cgtggctcac cagcaggtaa cctccgcgat gtctggccag ttgctggctt agccagtcga
     1801 tgccagaggc atcgaggtgg ttgtctggct catcaagcaa cagaaagtga gcaggcctcg
     1861 caaacagcgc cagcaggcgc aaacgggtga gctggccacc gctcagggtg ctgaccggga
     1921 catggggatc gagtctgagg cctgcttctg ccagcaggtc ggcaagatcg ctgcgcaggt
     1981 tccagcgatc agccagcaac tcgaaatcgt cctgggcgca gctcccctgc tcgatgcgct
     2041 caagggcccg tagcggctcg ccccagccga taaggtcggc cacactggct gcgcagtcat
     2101 gatcttgagc cggtgcaaac tgggacaacc agctgatggg ggtcaaccac tcgatcttgc
     2161 cagaatccgc gcggcgctgg cccgccagca gttccagcag caggctcttg ccgatgccgt
     2221 tacggccgat cagggtgcag cccgcagcgg gcaaagtgaa ggtaaggggc acagtacagg
     2281 cgacgaaggg cagctgcaaa gtgttgatat gacatgcgat atgggtcatc tgaactcctg
     2341 taggccaatg cggaagccgc aggccaagag ctaaaccaaa tgacagggac acgtgtccac
     2401 tagtgggaaa acagcagagg aatcagcatc aacctgtcac ggtgagacgc acccgctctc
     2461 cctggcggga gaagaatcaa aagtggggtg cggacaggtt aaatgcgcat cggcgaaata
     2521 ctctttactg atgaaagtaa aatcatactt cagatagtaa aaaatgccaa atgttccggt
     2581 atcactcact tgcatgttgt atcaataagt caagtgtcag tctgggctat acctcaactg
     2641 ggcgtcagtc ggctcactgg catcgcgtca gactagggcc gctttctgca ttatctgacg
     2701 cctaaacgtc aaactctatg gcctcaacct gacaggccgc atgcttagcg tacgattttg
     2761 tccgttttct ctaccgtccc cttccacatg gaatttttgt gacacttcaa caaaaacaaa
     2821 gaggatttcg ggttactgcg tttccactcc ggtcagttca gcagttgacg acggtgatac
     2881 agtcccgccc tgaacatcac ggatatagtt tgcctgacaa gcagccagaa taatgactgc
     2941 aagaagcaat aaaataatcc ctgttatctt tttcataacg actccttgtt gtgaaagaat
     3001 ggccattcat ccttaaacaa ccggcattca tatttcagcc ctgtttaaat cctggcttac
     3061 cgttagcagc tcgccattct ttaatttcct tgacaatccc ttcaggacta tcttccctgt
     3121 catcgcgagg ataataaatc agatctgaac catctgggtg ttcagttaat cgctcaaact
     3181 ctctcactaa tttattgtca tcctcttcag tagcaccttc agctctacat atttttttta
     3241 caaactccag aaattcagcc tcggtataat cactaatact atgtttcagt tccattttta
     3301 cttacctcga tgaatatcaa tatgtcgctt aggtgtggtc actctgatat tattcatatg
     3361 tcagggtggg atatttttgg cagttcggag gaatcactca tgaaaacggt gaacggaacg
     3421 cgaaatcctt tcgcgggtcg tgaggcgttg gacaagcgaa gcgcgtcagt ttttccggct
     3481 cttacccgtg acaggtttct ccgccagttt atcttccagt gtcacaactt tctgatacac
     3541 cgccgctgtg gcgttgtagt tcctgtcgag cattgttttc attccccatc cgagcacaca
     3601 gaacataacc agaaaagcca ctgacacccc acccagccat ttccagctat accctttaag
     3661 ggtgttgtat tcctgcacaa tattgctgtg aattgtcgca atatccctgt tcagggcatc
     3721 cgcgtttgcc ttcatgtttg cgactgactc ttccagccgc tgaaaatcct ggctgatgcg
     3781 ctcacctatc agccctgcta cgtccagctt ctgcacctcg tttctgactt catccctgac
     3841 ctgttttgcc acaccgtcag aaatatcccg atatccgcga agtgtttccc ttgccacagc
     3901 cagagaatca gcaacagctt tctgttgctc cattgcggta tgttgcagat gctccaccga
     3961 taacaggagc gtgagtaatt tttcttctgt gctggccatg ttcagaatcc catagaaaat
     4021 ccacgtgacg gtgatttaac ccgttcaggt tcagttcggt tctgtctgct cactctgtct
     4081 ctcatgtcaa cgccatgctc ccgcgcaaca tcatgttgca gccttctgac cagttcatgt
     4141 tgcttaacag gtgtatccag ccattccctg atgtcatgcc cgaccattcg cgcctgttca
     4201 agatggcggg aatagtccgg ttcagaccgt ttttgcatga tgtcatgacg ccgggcatca
     4261 tcaagccgtc cggcagcttc aaaacccaga tgcacttcgg gacgtcgttc aatgccctgt
     4321 tctttcagcg tccggcagtc aatgaagccc ctttcaaagc catgctttct catatgttcg
     4381 ttgtgcttca cctcccagcg ttttcgcaat tcccgcaact gctctttttg ctgagtggga
     4441 gtcaggcttc cggaatcctt tttcgcacct ccccgctcgg gatacctggc gttatagcgc
     4501 cggaaatact gttccggtgt acggtcaatg ccatcattca cacgctggct catcatgata
     4561 tgcgcatgag gctgttcgcc gccgtcaatg ctcgctttcg gattgtgaat ggcaaaactc
     4621 caggcgtgcc gttcgccggc ttcctgacgt acaaaatcct gcaccaattc aaggcgctgt
     4681 tccggtgtca gttcacgggg taatgcaatt tcaagttccc gataggtcga accgttcgca
     4741 cgttcaaact ggtcagccgc cagccagaaa tgggagggat tatctcgcgc ccattccggc
     4801 atgtttccgt gctccgtgta ctccaggtct ttccgtttcg catactctcc tttacgctcg
     4861 atgtaatcgg cgtggtttgc agcctgtccc ttgcttccaa atttgacgct gaggtgatag
     4921 gtcgccacgg tttcagccct ccgcaggagc gcactgtttc tcgacgaagg agagaaacat
     4981 ataagtgcgc ttccttctaa ttttacgaat tagcacggaa gataccccct gcacccgccg
     5041 accgcaagcg atatactgca cgaccacaac acaccgcaaa aaaacaccag ggagcaccac
     5101 gaacatgcgc atcagcaaca tcgaatggct gaagaaacgg ctcggattca tcaggaagct
     5161 cggagaacag accgcgcgac agagacagat tattgacctg ctcgacaacg aagccggact
     5221 cacagaacag gaacgcaaac ttctccacgt gctggcaaca gcggaaaaaa acgacttaca
     5281 ggcacaggag aacgagcgaa aacagacgat ccagaaacga atcgagggaa aaaaacaacg
     5341 acgggaacgc aatcaccgac tctttctcgc ggctgggcta ctgatcgaag caggactggt
     5401 agacacaaaa acaggcgagc tatgctataa aaaagacagg atattacagg cacttaagga
     5461 aattaagtac gatctggaga catccccaaa cccagacgcg taagtgcctc catccgtgtt
     5521 ccggttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     5581 agcactacca gcacaatcat gcggaaaaca gcaccattgc agggctgaac ggggaaaaac
     5641 ggcaaaagac ctactgctca aaaaaacgcc agaaaggcgc tgttatctga tataggctat
     5701 tttccgcttt tccagtttcg aatggttcga gtagaaacct ccaacatcgc cgcaatagct
     5761 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     5821 tcgcctttag cgatgccacc tttcctgcct ctcttggcct gtatttctgg cgtgtgcgta
     5881 tcagccacat actgtgcgaa agtttccggc gtgaagttcc tgtgcgtgta tttcgcaata
     5941 ctcttgccaa tagcccgaca ttcaggaggt gaaagcggaa cgggaagcga tgcgttgtac
     6001 atttcgacac gctgaatcac ggcatcaagc cactgtgaga atgctggcca gccctgacga
     6061 atcgccctgt aagcccattt gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg
     6121 taatgtttat cgatgctacg acgctctgag gcgctcaaat cgagataatc tgccagttca
     6181 tcgagggtat aggcgtcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     6241 ttgcaaatca ggccgctgta gttcacatca gcacaaagtt tctcacacag cgaacgttca
     6301 accgctgcgg catatttcag cgccttaacc gaggcatcag gcgcggttct cacggcgatg
     6361 ttgagtgcgt agagcaggtg agcgtgtccg ttcacaggat ttttaacggt gatgttcggc
     6421 gcgggggcgt tccggtcgct ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc
     6481 agccagaact gcgcatgagg ctggttctgc tggatgtacc gcgcaagaag cgcacgccct
     6541 ttgccggaga tgcgaaggcc gaaagcgaga tcatccgtgt gataggggcg gtggggtaaa
     6601 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     6661 cgacctcgcc cctgtattct cgtgacaatc ttggcggaga gacacgaatt
//
