LOCUS       NZ_CP115364             3603 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET20-1667 plasmid pCUVET20-1667.3,
            complete sequence.
ACCESSION   NZ_CP115364
VERSION     NZ_CP115364.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339843
            Assembly: GCF_030996505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:12:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,496
            CDSs (with protein)               :: 4,496
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 78 of 226
            Pseudo Genes (incomplete)         :: 145 of 226
            Pseudo Genes (internal stop)      :: 38 of 226
            Pseudo Genes (multiple problems)  :: 36 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115364.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET20-1667"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET20-1667.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2020-09-14"
                     /collected_by="Pattrarat Chanchaithong"
     gene            119..553
                     /locus_tag="PCS92_RS24215"
                     /old_locus_tag="PCS92_24200"
     CDS             119..553
                     /locus_tag="PCS92_RS24215"
                     /old_locus_tag="PCS92_24200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309203999.1"
                     /translation="MSRLYRAALPEDYVYNHETGLWELSFDQRPVAGTRADSPEEGAAQ
                     YNAAREKFKSAVSAVRQRPEPVFSFTAMTEWVVEHGTQEQCQAVQIMFDARDKHEFDLA
                     VRDGLKVLPGHLDFAYFFGRFSDRMRVSVLLREQLSQRLS"
                     /name="hypothetical protein"
     gene            2473..2931
                     /locus_tag="PCS92_RS24220"
                     /old_locus_tag="PCS92_24205"
     CDS             2473..2931
                     /locus_tag="PCS92_RS24220"
                     /old_locus_tag="PCS92_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_309203997.1"
                     /translation="MPPVKPALADDFSQGSVNTPEFVDLPYMKQEHVVALRNFIVQVAK
                     GQPLVGKNKPSWLGDNLQELPHTQSYKANNYWHYHCGPSYSNASVKSMTYNLNMNLEGL
                     TSPEVIHYEKKEDGSIIIVGFSPNHIPFPPSDLPGHSNPLFPYEETDD"
                     /name="hypothetical protein"
     Typing          3212..3308
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.755"
                     /coverage="89.0"
                     /evalue="3.35e-23"
ORIGIN
        1 gctcgttaac ctgtaacgct cgccgcagtg tttaactagc gaagtctgtt aaaccgagga
       61 agcggaatat caccgatgct gaggttttcg cttacacact gatgttgagg agtttccctt
      121 gtcccgtctt taccgtgctg cacttcctga agactacgtt tataaccatg aaacaggcct
      181 ctgggaactt tcattcgatc agcgtcccgt agcaggaacg cgcgcagatt ctcctgaaga
      241 aggcgccgct cagtacaacg ctgcccgtga aaagtttaag tctgctgtat ctgctgttcg
      301 tcagcgtccg gagcctgttt tttctttcac cgccatgacg gagtgggttg tggaacacgg
      361 tacgcaggaa cagtgtcagg cggttcagat tatgtttgat gcgcgggata agcatgagtt
      421 tgatctcgca gtgcgtgacg gtctgaaagt gctgcccgga catctcgatt ttgcgtattt
      481 ttttggtcgc ttttccgatc ggatgcgcgt gtctgttctg cttcgtgaac aacttagtca
      541 gcgtctttcc tgacgtttac tcagcgtaaa ctctgcggtc gctgcttcct gttttactct
      601 ctattttgac ccctattttt atctattggg acgcataggg agtaaaatag aggtcaaaat
      661 aggggggctg gcgcattgca tgcaccagaa agggacatga ttttctgaac gaaaaccacg
      721 tcccaagggt tgcgccatgc ggcttcaccc tctctgactc ctctgcggct gaacgccgcg
      781 ccgtcatcag attcacccgg ccccgtccga cgtctccgac gcctgaccac ccccttaaga
      841 cggttactcg cctggctcgt cacctttaag agggtggtca ggcgcctacg gcaccgacgg
      901 gggggcgttg cccccgctgc gcaaatcgtc catgggcgga ctcgcaacgc tggggcgttc
      961 cgttgcttgc ggctccccca aaagcacgat cccctgaacg ggtatcgcaa agcctgacac
     1021 cgcacccgta tgttcttccg gctctctgtt tttgctgcga tgcaggatag ggctgacgcc
     1081 gcaaagtgac gtcatctgac gttctctcgt taggtaattg ggacaggaaa tgttttgtca
     1141 ttaccgccgt tccgcgccgg cgacaaaaca gcgggacaag atgtgttttt ggtacaccgc
     1201 cggcgcacca aagcctcctg gtcaggctcc gcccgacacc ctgataaatt tcgacgcgtc
     1261 gaaatttatg ttcacccttt gacgaacgca ggatagaaca gcgtcagcaa gtggactgcc
     1321 gcgccttacg gcttggccaa cgcctcacgg ccgcacagta aactgcgcac ccgctcaccg
     1381 ttatgcgggg agttctttac cggaaatccg tctttagtgc cagcatccgt ccgaaatcac
     1441 ctgccattca ctcgcgtgca tagccatgca ggtgcgcaga aatcgtcagg cgcgtttttt
     1501 gacgatatgg tcagggaaag gtgccgaaaa cggctatctc gccgcagaat ggcgctgagg
     1561 gcctctcagg gggtgcgccc aagagatgtt atggtaaata gcagacctta atagaatgca
     1621 tctcattttt tcgatagctg aatggctatc aattgtctca tcaaggggtc aacgtcataa
     1681 gtgttagtca gccccacgtc atgcttactc tcggagcgga ttctctttcg aactaactgc
     1741 ttgtcttcaa gttttcgcat tgtacgttgt attgttcggc ggtcttgatt catcctatcg
     1801 ctcaagagct tgattgatgg atatattttt gcgaaaggac tatggatgaa agagataagg
     1861 tgaaggagca agactaactc tgagttactg atgttcagtt ttgattgtga cttcataaga
     1921 acattgggaa tggtggtcca cccttcgctt aagatgtttt gcccccatat tgcgaggatc
     1981 gtctggggca tctcttcttt taaaaccatg gtcaaacctc aaaaaaaatt ttttttcgca
     2041 tcactactct ataagaaata agtaagttac ttatttctta tagagtagtg atattattag
     2101 ggcggcgtca acgccgccca taaggactga attttatagt ccgtagctct aagatccttt
     2161 acaaagagtt ccgataaccg atagcactcg ctatgaatgc aacaccgttt gttttctcct
     2221 gcgaacaggc agatcggtaa gttttaccca ttcatccgaa cataaggtct caaatgttag
     2281 ccatggtaaa gtcacagcgt agctccagcg atgatgctcg cagcagacat tacaaaaagt
     2341 gtgctgatga ggtgcttttg tctcgcgcta attcaactgc ttcagtcgta tttaatgctg
     2401 aacagtcaga tgacgaaatc ctaaacatcc tgttatcatt ccagcatcga gaatgataat
     2461 tgggtgctat aaatgccacc agtaaaacca gcgttagcgg atgacttcag ccagggaagt
     2521 gttaacaccc ctgagtttgt agatctccct tacatgaagc aggaacatgt ggtggcttta
     2581 aggaacttca ttgttcaagt tgctaaggga caacctcttg tcggcaaaaa caagccatct
     2641 tggttaggcg ataatctcca agaactaccc cacacccaat cctacaaagc caataactac
     2701 tggcactatc attgcggccc cagttatagc aatgcatctg tcaaatcaat gacatataac
     2761 ttgaatatga atctggaagg tttaacctcg cctgaggtta ttcattatga aaagaaggaa
     2821 gacggaagca tcataatcgt tggattttca ccgaatcata ttccttttcc gccctctgat
     2881 ttaccaggac actctaaccc tcttttccct tatgaagaga cagatgacta atacctgatt
     2941 ttcttgacta ctgctttata gtgcgcgtag tcttttgggc agaaacgaaa aaaccgccct
     3001 gcaaggcggt tttttcgaag gttcagaaag ttggggaact tactgaacca cggtgactgg
     3061 ctttaacgga gcgcagtaac ctaatttgtc ctttcagtgt agccgcagtc aggctcctac
     3121 ttcaagactt cttgttaagt tagcttaaca tctcgctccg taaacctgtc accaatggct
     3181 accgccagtg gcgcttcgtc gtgtctgtca gggttggaat caagagaaca gttatctgat
     3241 aaaggcgcgg tagtcgggtt gaacggggag ttcgtgcata cagcccagct tggagcgaac
     3301 cacctacaag gaactcagtt gcacgggtag gaatgagaaa acgccacctt acggcggtct
     3361 gcaacgggta aacctcaggg tgggaacagg agagcgcacg agggagcgac ccatcggaaa
     3421 cggtggggat ctttaagtcc tgtcgggttt cgccccttct gatttgagcg ccagagatcg
     3481 cctgatttct gtgaagctcg tcaggagggc gaagcctatg gaaaaccggg gaaccggaag
     3541 gtcttgctcg gggggaaagg ggggcgtgct gttgatctta gccagagaag ccgtcatctc
     3601 ttt
//
