LOCUS       NZ_CP115363             6478 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Escherichia coli strain CUVET20-1667 plasmid pCUVET20-1667.2,
            complete sequence.
ACCESSION   NZ_CP115363
VERSION     NZ_CP115363.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339843
            Assembly: GCF_030996505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/07/2023 07:12:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,844
            CDSs (total)                      :: 4,722
            Genes (coding)                    :: 4,496
            CDSs (with protein)               :: 4,496
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 78 of 226
            Pseudo Genes (incomplete)         :: 145 of 226
            Pseudo Genes (internal stop)      :: 38 of 226
            Pseudo Genes (multiple problems)  :: 36 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115363.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET20-1667"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET20-1667.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2020-09-14"
                     /collected_by="Pattrarat Chanchaithong"
     gene            1..840
                     /locus_tag="PCS92_RS24170"
                     /old_locus_tag="PCS92_24155"
     CDS             1..840
                     /locus_tag="PCS92_RS24170"
                     /old_locus_tag="PCS92_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1679
                     /gene="repC"
                     /locus_tag="PCS92_RS24175"
                     /old_locus_tag="PCS92_24160"
                     /pseudo=""
     CDS             827..1679
                     /gene="repC"
                     /locus_tag="PCS92_RS24175"
                     /old_locus_tag="PCS92_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /name="repC"
     gene            1831..2685
                     /gene="estX"
                     /locus_tag="PCS92_RS24180"
                     /old_locus_tag="PCS92_24165"
     CDS             1831..2685
                     /gene="estX"
                     /locus_tag="PCS92_RS24180"
                     /old_locus_tag="PCS92_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="WP_094315607.1"
                     /translation="MKEKVVVDKAISLHTESFGDPAHEPIILIMGAMASAVWWPDEFCS
                     QLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDGYGLEAAHLVGMSLG
                     GFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQRAESLDWSDRDAVV
                     AYQVGAWRIISGTAHAFDAEKIQNIAELDFDRTPNILTTFNHTTSGGGERSLERWLGRL
                     NEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHHEDWPRIIQAIKG
                     QTS"
                     /name="estX"
     gene            complement(3355..3639)
                     /locus_tag="PCS92_RS24185"
                     /old_locus_tag="PCS92_24170"
     CDS             complement(3355..3639)
                     /locus_tag="PCS92_RS24185"
                     /old_locus_tag="PCS92_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            3944..>4477
                     /locus_tag="PCS92_RS24190"
                     /old_locus_tag="PCS92_24175"
                     /pseudo=""
     CDS             3944..>4477
                     /locus_tag="PCS92_RS24190"
                     /old_locus_tag="PCS92_24175"
                     /inference="COORDINATES: protein motif:HMM:NF015354.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            4587..5000
                     /locus_tag="PCS92_RS24195"
                     /old_locus_tag="PCS92_24180"
     CDS             4587..5000
                     /locus_tag="PCS92_RS24195"
                     /old_locus_tag="PCS92_24180"
                     /inference="COORDINATES: protein motif:HMM:NF036929.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            5045..5968
                     /locus_tag="PCS92_RS24200"
                     /old_locus_tag="PCS92_24185"
     CDS             5045..5968
                     /locus_tag="PCS92_RS24200"
                     /old_locus_tag="PCS92_24185"
                     /inference="COORDINATES: protein motif:HMM:NF028103.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_014344452.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            6029..6241
                     /locus_tag="PCS92_RS24205"
                     /old_locus_tag="PCS92_24190"
     CDS             6029..6241
                     /locus_tag="PCS92_RS24205"
                     /old_locus_tag="PCS92_24190"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            6243..6449
                     /gene="urfF"
                     /locus_tag="PCS92_RS24210"
                     /old_locus_tag="PCS92_24195"
     CDS             6243..6449
                     /gene="urfF"
                     /locus_tag="PCS92_RS24210"
                     /old_locus_tag="PCS92_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     Typing          3594..3750
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.38e-77"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          3840..5965
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggattc gagttcagcg gcccggagcc gctgggcgct gatgatctgc
     1021 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac
     1081 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca
     1141 ccgctgatgc catggtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag
     1201 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg
     1261 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg
     1321 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg
     1381 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg
     1441 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca
     1501 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca
     1561 tgcgcaagcg ccgccagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga
     1621 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac
     1681 cccccccact ctattgtaaa caagacattt ttatctttat aaaacaattg gttggggatg
     1741 tttgagcgca attaacaggc agccaaaact ggcaaaatga gcatgcctat gaataaaatg
     1801 ttgtacgtaa aactcgaagg caattttagt atgaaagaaa aggtagttgt tgataaagcg
     1861 atttcactcc ataccgaatc attcggcgac ccggcccatg aacccattat cctgatcatg
     1921 ggggcaatgg cgtctgcggt gtggtggcct gatgagtttt gctcccaact agccaaaatg
     1981 ggtcgctatg tgatccggta cgaccaccgt gataccggga aatcaacaag ctatgagcca
     2041 ggtcaagctc catattccgt tgaagaatta gcagatgatg tggttcgcgt cattgatggt
     2101 tatggtctgg aagctgctca tttagtcggc atgtctttgg ggggatttct ttcccagctt
     2161 gtagctctca agtatccgaa acgtgtgaag agcttgacgc tgattgcttc agaacggctt
     2221 gcagatgcag atcctgatat gcccgctttt gatcctgcca tcattgagta tcaccaacgg
     2281 gcggaatcgc tggattggtc tgatagagat gctgtcgtcg cgtatcaggt cggagcgtgg
     2341 cgaatcatct caggtactgc gcatgctttt gacgctgaga agatacaaaa tatcgctgag
     2401 ttagattttg atcgcactcc gaatatcctg acaacattca accacactac ttcaggtggt
     2461 ggcgagagat cgctcgagag atggctcggg agattaaatg agatagctgt accaactttg
     2521 atcattcacg gcacggagga tcctgtactt ccttatgtgc atgggttggc actgaaagat
     2581 gcgattcgtg gttcaaaaat gctgacactc gaaggcacgg gacatgagtt gcatcatgaa
     2641 gactggccga ggattatcca ggcgattaag gggcaaacgt catagctatc ggttgcaccg
     2701 tacaacaagt tgctgcaccg taaaaattac tcgctgcgct cgcaattttc cggtgagcaa
     2761 ggcgttaaac atcatgaggg aagcggtgat cgctgaggca tgttccgcgt ccttgcaata
     2821 ctgtgtttac atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc
     2881 tgccgttgct agacattgcc agccagtgcc cgtcactccc gtactaactg tcacgaaccc
     2941 ctgcaataac tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac
     3001 gcccccaaac ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc
     3061 catgattatc taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct
     3121 gcccttgccc aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct
     3181 acacaccgcc ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac
     3241 accaaacccc gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta
     3301 cccgaagggt gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc
     3361 cggctcatcc ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag
     3421 gtacgcatcc attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc
     3481 caaaatcatg gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg
     3541 ttcccttgcc cgcacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt
     3601 ggccagccga tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg
     3661 tgctgtctcg taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg
     3721 gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg
     3781 gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat
     3841 ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc
     3901 caaggccgac tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca
     3961 cgccgaatcc gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc
     4021 cgacctgtat gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt
     4081 cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgactgg
     4141 tgccgagcgc ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg
     4201 ccacctgatg atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt
     4261 caagcggtac aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa
     4321 gcccaaggca tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt
     4381 agagcgggct ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga
     4441 gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg
     4501 caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt
     4561 acaggaatac cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag
     4621 gcatcaacga gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg
     4681 acgaagcccg gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt
     4741 ggctgaaagc ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg
     4801 aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc
     4861 actggaagct atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga
     4921 tcgcatcgtt gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc
     4981 tgcgcttggt ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa
     5041 ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg gccagatgat
     5101 gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa
     5161 tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct
     5221 ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc
     5281 cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc
     5341 aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc
     5401 ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa
     5461 gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac
     5521 cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg
     5581 gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg
     5641 ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt
     5701 cggtgatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg
     5761 cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa
     5821 gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag
     5881 cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga
     5941 caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta
     6001 tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt
     6061 ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa
     6121 caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg
     6181 caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg
     6241 atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac
     6301 gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc
     6361 tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg
     6421 gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatca
//
