LOCUS       NZ_CP115355             1516 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET21-37 plasmid pCUVET21-37.4, complete
            sequence.
ACCESSION   NZ_CP115355
VERSION     NZ_CP115355.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339846
            Assembly: GCF_030996445.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:56:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,914
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 282
            CDSs (without protein)            :: 282
            Pseudo Genes (ambiguous residues) :: 0 of 282
            Pseudo Genes (frameshifted)       :: 117 of 282
            Pseudo Genes (incomplete)         :: 178 of 282
            Pseudo Genes (internal stop)      :: 48 of 282
            Pseudo Genes (multiple problems)  :: 55 of 282
            Pseudo Genes (short protein)      :: 2 of 282
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-37"
                     /isolation_source="blood"
                     /host="cat"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET21-37.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-01-07"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(1488..1516,1..442)
                     /locus_tag="PCS94_RS24565"
                     /old_locus_tag="PCS94_24520"
     CDS             join(1488..1516,1..442)
                     /locus_tag="PCS94_RS24565"
                     /old_locus_tag="PCS94_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_308398617.1"
                     /translation="MHGIFRGKKSSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRELL
                     KDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNALVR
                     IPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(886..1107)
                     /locus_tag="PCS94_RS24570"
                     /old_locus_tag="PCS94_24525"
     CDS             complement(886..1107)
                     /locus_tag="PCS94_RS24570"
                     /old_locus_tag="PCS94_24525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_158414302.1"
                     /translation="MCKCNTVTRDDIPFAVWSGLRPDTPHQCVRAQCGFRFSSASSPTR
                     SLRSVAGAAASGVTSRMLSVIPLLFFRG"
                     /name="hypothetical protein"
     Typing          1047..1127
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="87.0"
                     /evalue="9.59e-28"
     Typing          complement(1330..1438)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(675..933)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="1.09e-111"
ORIGIN
        1 ttcagatgag gcaaagaaaa acaccgagga tctcgaagaa gcaaagaaga attcaaggtt
       61 tacacaggta tccccaaaag gttgggaacg cgttcgagag ctgctgaagg atagtcaagg
      121 catatcagca ctgaagctgt actcattttt agcggagcat atcgatccta cgtgtggcgc
      181 tgtcgttgcg gatcagcaat tcctagctga aaaacttgga gttagcagaa gcacaattat
      241 tcggtggctc aattacttag aatcaaaaaa tgcattagtt agaatccccg ttgctggtaa
      301 ggtttgtgcg tatgccctcg atccacatga agtctggaag ggatacaaca ctacgaaaaa
      361 ccatgcagcg tttgtcacta aaacactggt caacaaagac ggtgatattc agcgccgaat
      421 catggccatg ttttcaaatt gagccagcgg caggcggaca atcaggggct acgtgttaac
      481 gttttgacca tgattgtcta tcctgcattg ctcttttgcc gcctcaaaat cccttgcttg
      541 tttttgctcc ccgatcccca gaacgaccgc gccgccacgg ttccggcagc gccttgagcg
      601 caaccggaaa ccgtctgggg agaaccccag acccccatcg ctgtaacacc gacggtctac
      661 gaccaccaac cagccgaata gctgcctgtg ccgtgcaaat aggctttgca gggggctgac
      721 gcggttccta gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgag
      781 agcctctgta agcgtttttc gcgccttcca tgccctgacg ggcatttaaa acccgtttcg
      841 tgtgtctgag agcttctgag cgcgtttgag gggctatctg gcgactcatc cccgaaagaa
      901 caacaacgga atcaccgaca acatgcgaga ggtgacaccg ctcgccgcag ccccagcgac
      961 cgaacgtagt gagcgagtgg gcgaggaagc ggaagagaac cggaaaccac attgagcacg
     1021 tacgcactga tgcggggtgt cggggcgaag ccctgaccag acggcaaacg gaatatcgtc
     1081 gcgggtgacg gtattacatt tgcacatcct gtcccgattt ctgaggcgtt ttagcggtga
     1141 acggacggca aaaccatgat ggagctgttg agcgttgaga gcgtgaaatc tggcaaaaat
     1201 cacggctggc gaggcttgta gcggaataag ccagtataca ctccgctatc gctacgtgac
     1261 tgggtcaggg cttcgccccg acaccccaaa agggcgttgc gttgcgcgca acacccttgc
     1321 ctagaataga tcttttacgc cgatttgtaa gttgttgttt ttttagtgtg ttttttgttt
     1381 acctgtcgca tccagggtga cacaacgttg tcgcatccag gatgcgacaa cacgcaacaa
     1441 ggtgttgcac agatgtgtca tgatggctta gatttgaacc aacgttagtg catgggattt
     1501 tcagagggaa aaaatc
//
