LOCUS       NZ_CP115350             1532 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET21-H2 plasmid pCUVET21-H2.7, complete
            sequence.
ACCESSION   NZ_CP115350
VERSION     NZ_CP115350.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339847
            Assembly: GCF_030996425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:05:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 87 of 230
            Pseudo Genes (incomplete)         :: 156 of 230
            Pseudo Genes (internal stop)      :: 44 of 230
            Pseudo Genes (multiple problems)  :: 53 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115350.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-H2"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET21-H2.7"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-03-22"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(539..982)
                     /locus_tag="PCS86_RS25600"
                     /old_locus_tag="PCS86_25525"
     CDS             complement(539..982)
                     /locus_tag="PCS86_RS25600"
                     /old_locus_tag="PCS86_25525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_192931344.1"
                     /translation="MKNTEGSCKLNELVSVAANNRLHRNIEEVENLNEPEEARLMAGFF
                     RSTRLQRNKQVQKRNLSAIAHQIYKKRTFTRPNSQKRTRNVFTRLNNYSRTRSADGFDG
                     STARTGQNGKLSHQKTVNGTRNFFAGREALAGCRGAALTRWQL"
                     /name="hypothetical protein"
     Typing          74..154
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.683"
                     /coverage="96.0"
                     /evalue="2.04e-24"
     Typing          complement(491..578)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="96.0"
                     /evalue="2.61e-33"
ORIGIN
        1 gaacgtagtg agcgagtggg cgaggaagcg gaagtgaacc ggaaaccaca ttgagcacgt
       61 acgcactgat gcggggtgtc ggggcggatg ccctgaccag gtggtaattg tgatagcgtc
      121 gcgtgtgacg gtattacaat tacacatcct gtccagaaaa ttcccgccgt ttctcggttt
      181 ttttgcgcgt cgcagaccgc acgacaaacg aaacgaagtg aagtttgtat aagtgcgcac
      241 acttcgtgag ttttctgtac tgccagtttg tcgattgcgc ggaacggctg cgtcacgaaa
      301 acggcaaaat gatttgctgt ccggtcgccg aaatttttgc gtagctgtgg tggctgcggt
      361 gccaaaaatc atccgcgcgt taagtgcgga tgcgcactta gtagtttctc tccttcgtcg
      421 ggaaactgtg cgctcctgtg gaggggttaa cggcgcagaa ctgccgatgc tctgccagcg
      481 ttctggcgaa aaaatcggga caggatgtgc aattgtaata ccgtcacacg cgacgctatc
      541 acaattgcca tctggtcagg gctgcgcccc gacaccccgc caacgcctca cggcccgcga
      601 aaaaattccg cgtcccgttc accgttttct gatgagaaag tttgccgttt tgacccgttc
      661 gcgccgtcga accgtcgaaa ccgtccgccg atcgtgtgcg tgaatagtta ttcaggcgcg
      721 taaaaacgtt tctggtgcgt ttttggctgt ttggtcgggt gaaagtgcgt tttttgtaga
      781 tttgatgtgc tatcgcgctc aaattgcgtt tttggacttg cttgtttcgt tgaaggcgag
      841 tagaacgaaa aaagcccgcc attaggcgag cttcttcggg ttcattgagg ttttcgacct
      901 cctcaatgtt cctgtggagt ctgttgttcg cagcgacaga aaccagttca ttcagtttgc
      961 atgaaccttc tgtattcttc aagaattttc ccgccgcaca gtctcccagg aatcccgaaa
     1021 aaacctgttt ttgcaggcac ttgttgcgtg tttgcaaagg ccgtttgcgg ttggggggtc
     1081 agtgacgacg gcgaccgcac gcgtgagaat tccgctaccg gggaagatac ttcatttttg
     1141 agcggacgcc tggggagtgg cacaagtggt tgaatgtaat gcgcttatat tgggtttttg
     1201 cccttgggcg cggttctggg accagtgcca gcatgttggg cgaaatgtcg cggcgtagac
     1261 gcgggaacat gcaaacggac gttctaccat aggtccgttc cggtgacgat gggcgcggct
     1321 ccggccagca tatcgctcgg gcaaaactct tacccccttt tacgcccttt gcggggtggg
     1381 gggggagttt gccggttcct cagaacgctc accaaccagc atatggcaca gccccctgcc
     1441 gcaccgcaat cacagactgc tctaccgcaa aaaaaaaaga tccctacagg atcgagagag
     1501 agatcactcc gctcgccgca gccccagcga cc
//
