LOCUS       NZ_CP115348             1989 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET21-H2 plasmid pCUVET21-H2.5, complete
            sequence.
ACCESSION   NZ_CP115348
VERSION     NZ_CP115348.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339847
            Assembly: GCF_030996425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:05:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 87 of 230
            Pseudo Genes (incomplete)         :: 156 of 230
            Pseudo Genes (internal stop)      :: 44 of 230
            Pseudo Genes (multiple problems)  :: 53 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115348.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-H2"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET21-H2.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-03-22"
                     /collected_by="Pattrarat Chanchaithong"
     gene            411..1397
                     /locus_tag="PCS86_RS25590"
                     /old_locus_tag="PCS86_25515"
     CDS             411..1397
                     /locus_tag="PCS86_RS25590"
                     /old_locus_tag="PCS86_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_061672792.1"
                     /translation="MENPELQNLTDYSPSDAPWDAHRSASDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLSRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHARWVELWRECLRVDYDPSVDVRAVKPRKPKDGE
                     SLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLKL
                     DQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          1400..1481
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="90.0"
                     /evalue="7.3e-29"
     Typing          complement(1124..1315)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 tgaggccaac cacctgcaag acggttttgt tgttcacaaa atcagtggaa aagcctgtga
       61 gtgttttgtc gggaaaacgc tttcggggtt gttgtttttg ccgccagaaa atcagctcag
      121 attttgagca aatctcgcgc gcgatccttg tatttatact taagggataa ctctgggggt
      181 aaaaaatagc tacaggcctt gtgtggcaag gctttgcggc ttatttgcgt tgtagaaggt
      241 tttagaagta ttctgtcgaa agtggtcgca ttaaaagcga cccccgcaac tgcgctaaca
      301 gttcgggggt gtgaccacct gtaggaattc gaattggtga cacgacaaca caggtgatat
      361 ggcaatgata acacgtcaga tgagccatcg cccacaatcg ggtggtgtgc atggaaaatc
      421 ctgaactcca gaacctcacg gactattccc cctcagatgc gccatgggac gctcacagaa
      481 gcgcttctga cgacgtggga gggatttacc tgcttgcagc tgaatatgag cgttacgggg
      541 cgcgtatggc ctcctgtggc ggtttactgc gttttgggtg gtctaccctc aaggaaaccg
      601 gagaaacgcg actgaggctg cgtgaggcgc atttctgccg tgtccggcac tgtcctgtct
      661 gccagtggag gcgctccctg atgtggcaag cccgttttta tcagtctttg ccgcgtattg
      721 tggctgacta tccagacgca cgatggatgt ttctgaccct gaccgtgcgc aactgtgcga
      781 taggggagct gggcgagaca ctgagccgaa tgaacatggc cttccagcga ctgaaggatc
      841 gcaaagagtt tcgacctgtt cagggctgga tccgcacgac ggaggttact cgaggcagcg
      901 acggtagcgc acacccgcac ttccataccc tgatgatggt gccgccctcg atgttcacgc
      961 gtgactatgt caaacatgcg cgctgggttg aattgtggcg ggagtgtctg cgggtagatt
     1021 acgacccgag tgttgatgtc cgggcagtga aaccccgcaa gccgaaggac ggcgaatcgc
     1081 tggcctgcgc gaccgccgaa ctggtgcgcg gtgcggtggc cgaaaccctg aaatattcca
     1141 cgaagccggc tgacatggtg gccgatcctg aatggttcct cgaactgact cgccagacgc
     1201 acaaacgccg ctttgtggcg accggcggcg cactcaaaga cgtgctgaaa ctcgatcagg
     1261 aaaccgacgc cgacatggtg atcggggatg atatttccga gggcgacgat gacggttcac
     1321 gcatcgcgtt cgagtggaaa accgaggtca gaaagtaccg tcgctcaccg acgaaagata
     1381 aaactgaatc aggctagcgg ggtgtcgggg tgcacccctg accagatggc aattgtgata
     1441 gcgtcgcgtg tgacggtatt acaattgcac atcctgtccc gaaaattctc gccgtatctc
     1501 ggttttttga gcgtagcagg ccgcaggaca aacgaaacca atgcaggata gacaatcatg
     1561 gtcagaacgt taacacgttg cccagattgt ccgcctgccg caggctctgt ttttaatttt
     1621 cggtgaaaac aaaaattaaa attctggatt ttggaaagag tgtgttttta aaaacacgcg
     1681 tttttgattt ttgaaaagag cctgcggcag gcacaggttg ataatccgat gacaaatgcc
     1741 caatttgaaa attggtaaca attgacatcg gtttgtctat cctgcattgg tttcgttcat
     1801 cgctcagggc tgcgccccga accccgccaa cgcctcacgc tatcagatta aaaatctgtc
     1861 agcgttcacc gttcccgaat tcgtcaaaca gaaaatcgcg gggtcccgcg cctttggcgt
     1921 gggttccgat tttgtgattg acgacggcac gggaaaaaaa cactggggat ggtggttggt
     1981 gtcacgcag
//
