LOCUS       NZ_CP115347             2113 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET21-H2 plasmid pCUVET21-H2.4, complete
            sequence.
ACCESSION   NZ_CP115347
VERSION     NZ_CP115347.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339847
            Assembly: GCF_030996425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:05:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 87 of 230
            Pseudo Genes (incomplete)         :: 156 of 230
            Pseudo Genes (internal stop)      :: 44 of 230
            Pseudo Genes (multiple problems)  :: 53 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115347.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-H2"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET21-H2.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-03-22"
                     /collected_by="Pattrarat Chanchaithong"
     gene            423..1388
                     /locus_tag="PCS86_RS25580"
                     /old_locus_tag="PCS86_25505"
     CDS             423..1388
                     /locus_tag="PCS86_RS25580"
                     /old_locus_tag="PCS86_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_196106741.1"
                     /translation="MSEDKFLSDYSLRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1392..1658
                     /locus_tag="PCS86_RS25585"
                     /old_locus_tag="PCS86_25510"
     CDS             1392..1658
                     /locus_tag="PCS86_RS25585"
                     /old_locus_tag="PCS86_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          817..1048
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tgaggccatc cacctgtaag acagggtttt gtttttattc cctgttttgg tgatcgggtg
       61 tgtggaaaag gttggggtaa gccgttcggg ggtgcttgtt ttggggggtt aaaattgtgg
      121 ttattttttg cgcaattctc gcgcgtgatc cttgtattta tacttaaggg ataaatggcg
      181 gatatgaaat agtggtttag cccagtaatg acgaggcttt gagtgggttt tgacaggtca
      241 aagaaaatgg agcagaattg aggcgttttt aatcggcgtt ggggagtgcg tcaacactcc
      301 ccaacatttc gaatgtgtca cctcagcggc aaactctggt gacatgtact ggctcgcaat
      361 gcacaggtac gtgatgaata taccacatca aatcacagcc tgcccagatc ggagcaggct
      421 taatgtcaga agataaattc ctttcggact acagcctccg tgatgcagtt tgggataccc
      481 agcgcacgct taccgattct gtcgggggta tctaccagac tgctgctgaa ttcgagcgct
      541 atgcactccg tatggcctcc tgtagcggtt tgttacgttt tggttggtct accatcatgg
      601 aaaccggaga aacgcgccta cggcttcgta gtgcgcaatt ttgccgtgtc cgtcattgcc
      661 ctgtctgcca gtggagaaga accctcatgt ggcaagcccg tttttatcag gctctaccga
      721 aaatcgttgt ggattacccg tcttcccgat ggttgtttct gacgttaact gtcaggaact
      781 gcgagatagg tgaacttgga acagtcctta cagcaatgaa tgcggcgttt aagcgaatgg
      841 aaaagcgaaa ggagctatca cctgttcagg ggtggatcag ggctacggag gtgacgcgag
      901 gtaaggatgg cagcgcacat ccgcattttc actgtctgct gatggtgcaa ccttcttggt
      961 ttaaagggaa gaactacgtt aagcacgaac gttgggtaga actctggcgc gattgcttgc
     1021 gggtgaacta tgagccgaat atcgatattc gggcagtaaa aactaagaca ggtgaggttg
     1081 tggccaacgt tgccgagcaa ctgcaaagcg cggttgctga aacgctgaaa tactccgtta
     1141 aaccggaaga tatggcaaac gatcctgagt ggtttcttga gctgacgcgg cagcttcaca
     1201 agcgccgttt tatctcgacc ggtggggcgc taaaaaacgt cctccagttg gatcgagaaa
     1261 ccaatgagga tcttgtcatt gccgacgatg taggggatgg cactgatgac gggaagcgga
     1321 cggcgtttgt ctgggattca ggtaaacggc gttacaaacg cgcccctgag aaggataaat
     1381 cggattaacg tatgaatatt aatattgaat acctgaatgg aaataagact attggtttat
     1441 tttttttaag aagtgaagcg gtgattcctg acaggtttaa aaaccttatt ttgcttattg
     1501 atggattaag ttttggcaca tttggttttc atccgcacga aggttttgag gatgaattaa
     1561 ttttatatat tcagaaaaca aacgagaggg taaaaactct ttttgtgaaa atagatttaa
     1621 acggtatcaa gtttggattt ttaagaacgc attcttagtt ctggaaaaga gccagcggca
     1681 ggctgaggtg ataggtacga gattgcatgc aatctctagt gctctgtcta tcctgcatta
     1741 tcctcagcat tatcctcagc attatcctca gcattatcct cagccttgcc aactcgacac
     1801 caatgcagga tagacaatcc gatgtcaaat gttaacactc tgcgagtggt acattttccc
     1861 cggattatcg tcctgagcct gccgctggct ctctttctac cgcctcgctt tgctcgttgc
     1921 tcaacgcctc acagacacgg attaaaatcc gcatccgttc accgtttttt aaagtccgtt
     1981 aaaagcatga tgccatctcc gagagttaat ctcgtcaaat gctaaatcgt gggggtcccc
     2041 tttggggttc cgatttagtg attgacgaca ccaccgatta aaaaacttat gcggggtgga
     2101 tggtttcacg aag
//
