LOCUS       NZ_CP115345             5570 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET21-H2 plasmid pCUVET21-H2.2, complete
            sequence.
ACCESSION   NZ_CP115345
VERSION     NZ_CP115345.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339847
            Assembly: GCF_030996425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:05:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 87 of 230
            Pseudo Genes (incomplete)         :: 156 of 230
            Pseudo Genes (internal stop)      :: 44 of 230
            Pseudo Genes (multiple problems)  :: 53 of 230
            Pseudo Genes (short protein)      :: 4 of 230
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115345.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-H2"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET21-H2.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-03-22"
                     /collected_by="Pattrarat Chanchaithong"
     gene            574..735
                     /locus_tag="PCS86_RS25515"
     CDS             574..735
                     /locus_tag="PCS86_RS25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(927..1883)
                     /locus_tag="PCS86_RS25520"
                     /old_locus_tag="PCS86_25445"
     CDS             complement(927..1883)
                     /locus_tag="PCS86_RS25520"
                     /old_locus_tag="PCS86_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_232251742.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTVENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDLPYVVNYYRNRLSALFD"
                     /name="type II restriction endonuclease"
     gene            1954..3093
                     /gene="dcm"
                     /locus_tag="PCS86_RS25525"
                     /old_locus_tag="PCS86_25450"
     CDS             1954..3093
                     /gene="dcm"
                     /locus_tag="PCS86_RS25525"
                     /old_locus_tag="PCS86_25450"
                     /EC_number="2.1.1.37"
                     /function="DNA methylation"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_031942531.1"
                     /translation="MTDNIAATIKEKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            complement(3459..3692)
                     /locus_tag="PCS86_RS25530"
                     /old_locus_tag="PCS86_25455"
     CDS             complement(3459..3692)
                     /locus_tag="PCS86_RS25530"
                     /old_locus_tag="PCS86_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3699..4217)
                     /locus_tag="PCS86_RS25535"
                     /old_locus_tag="PCS86_25460"
     CDS             complement(3699..4217)
                     /locus_tag="PCS86_RS25535"
                     /old_locus_tag="PCS86_25460"
                     /function="essential for mobilization of the plasmid"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_031942530.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4255..4953)
                     /locus_tag="PCS86_RS25540"
                     /pseudo=""
     CDS             complement(<4255..4953)
                     /locus_tag="PCS86_RS25540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4895..5218)
                     /locus_tag="PCS86_RS25545"
                     /old_locus_tag="PCS86_25470"
     CDS             complement(4895..5218)
                     /locus_tag="PCS86_RS25545"
                     /old_locus_tag="PCS86_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5283..5474
                     /locus_tag="PCS86_RS25550"
                     /old_locus_tag="PCS86_25475"
     CDS             5283..5474
                     /locus_tag="PCS86_RS25550"
                     /old_locus_tag="PCS86_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_137420181.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDDENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          100..646
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.182"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3356..4905)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.947"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
       61 cggcatcgat gcggattgta tgaaaagtgc gccatggacg gtgtgaaatg ccgcacagat
      121 gcgtaaggag aaaatgcaac ttccgacact cttccgcttc ctcgctcact gactcgctac
      181 gctcggtcgt tcggctgcgg caagcggtgt cggctcactc aaaggcggaa atacggttat
      241 ccacagaatc aggggataag gccggaaaga acatgtgagt aaaaaggcaa agtgcaacaa
      301 aaggccgcgc cgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
      361 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
      421 cccggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
      481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggc
      541 ttggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
      601 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgccttaacg
      661 ccactggcag cagccactgg taaccggatt agcagagcaa gatttatcca gatgcttaca
      721 gagttcttga agtgatggcc taactacggc tacactggaa ggacagtatt tggtatctgt
      781 gctctgctga agccagatta cctcggttag ataatctgcc aggattagct aaccttcgaa
      841 aaaaccacct gccagggtgg ttttttcgtt tacaggtcca agattacgcg cagaaaaaaa
      901 ggatctcaag agatcctttt agttttttag tcaaaaagag cactaagcct atttctataa
      961 taattaacaa cataaggtaa atcactatat ttttctattt gttttaatat tgactgttga
     1021 atttcctctt tttcagagtc tgtgtaactt acattattcc actttcttga taattccatt
     1081 gctgactgta gcatatcttc aaaggttagc accctattat tatttttata tttgaaattt
     1141 ttattttcta ccgtcgttac aacgaccaca ttagcctcgt acaatatatt tattgtctcc
     1201 tcgctaaaag aatcatctag tgtagctaga tacatttctc taattcctgt tctattaacc
     1261 tcttcaggta cttcttgcca tcgctccctc agcgttgttt tagcggaaat caacattgta
     1321 tttcttttgt tagatgtgta ctggactaca cctggcataa caagatctac aagtttacct
     1381 atttggtttt tttgaaaaaa tgacttccca atagccccct gcacatcaac aggaattcct
     1441 gcccccatca ttaatagctc aagaatagac tcaaactctt ttcctgccct gctacgtctg
     1501 gattgagtgt tagacagtgc caaatcatat atgtgtgtag ggtattccat cgtaaaatca
     1561 cgaatggcat cataaggact catatctttt attctttctt catcaataag atatgataac
     1621 attcttgttg tgaattcttt ttcaaatggt tcaaattctt tccaacagtt ttttctcatt
     1681 gcaattatat attcacttgg attattcaaa aaataatcac tatttttttg atcataccct
     1741 aaatcacaaa atgttttata aaccaaagca aatggcgtaa taaaatgcga ctttctctct
     1801 ttagaaatta gagttttaaa ttcaccagga cttaatcttt gcataaaaaa taacctttta
     1861 tatctacttg aaagtttttt catgcacgtt cataattgga atcaaaacag gacaaattgt
     1921 cctaaaacca acacttaatt ctggtagaca tgcatgactg ataatatcgc agcaacaatc
     1981 aaagaaaaaa gagaaagact tcatatgact caaaaagagt ttgcagacgc ccttggtctt
     2041 tcaaaatatg gagacagaac cataagaaga tgggagcgcg gggaaacaaa acctactggg
     2101 gcggaactaa aagcagttat ggactttcca gacacaccac cttacccaaa caacgagaat
     2161 ggcagatatc gcatgatcga tttatttgct ggaatcggtg gaaccagact tggatttcat
     2221 caaactaacg ctgtaaatgt tgttttttcc agcgaatggg ataagtttgc tcaaaagaca
     2281 tatcatgcaa attatggtga tttccctgat ggggacataa caaaaatcga tgaaaaagat
     2341 attcctgatc atgaaattct tgtcggtggg tttccttgcg tagcattctc tcaagcaggg
     2401 ctcaaaaaag gatttaatga tacaagagga actttgtttt ttgatatagc aaggataata
     2461 aaggaaaaaa aacctcatgc ttttttactt gaaaatgtaa aaaacttgct agggcatgat
     2521 aaaggtagaa ctttttcaat aataaaaaat acacttgaag aattgaatta cactgtgtat
     2581 tacaatatct tcgctgcaaa agactttggt gttccacaaa atagagaacg catatacatt
     2641 gtaggcttta ataaagaaaa agtaagaaat catgaacatt ttacttttcc aaccccatta
     2701 aaaacaaaaa caagagttgg agatattctt gaaaagagcg tagacaataa atatacgcta
     2761 tcagatgcgc tctggaatgg acaccaacgc aggaaacttg taaatgcagc agccggtaaa
     2821 ggctttgggt atggtttatt taatgaaaat agcccatata ctaataccat aagtgcgagg
     2881 tactataaag atggtagcga aatattaata gagcaaaaag gaagtaaccc aagaaaaata
     2941 actccgcggg aagcgtcaag acttcaaggg tttcctagtg attttattat ccctgtatcg
     3001 gatacacagg cttataagca atttgggaat tcagtcgcag tccctgttat caatgcaata
     3061 gcagaaaaaa tcatatcgac cttagattca taaactaagt cataaggaca caatgcggct
     3121 cagaatccaa ttcccccccc atatggattt accataacgt cgcattacgc gcaccgctaa
     3181 aacgcgttca gcacgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa
     3241 actgccgcga tcgcgcctga taaattttaa ccgcatgaat acctatgcaa ccagatggca
     3301 caggccacat taacccctct taatccactg aagctgccat ttttcatggt ttcaccatcc
     3361 cagcgaagga ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga
     3421 taatgcatgt tcgcgctcct gtatcagccg ttcctctctt atctccagtt ctcgctgtat
     3481 aactggctca agcgttctgt ctgctcgctc aagtgctgca cctgctgact caactgcatg
     3541 acccgctcgt tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt ctgcaattcc
     3601 acgaaggcgc tctcccattc gctcagccgc tgcatatagt cctgttgcag ctgctctaag
     3661 gcgttcagca aatgtatttc cagctctgtc actctgtgtc actccttcag atgtacccac
     3721 tctttcccct gaaagggaat cacctccgct gatttcccgt acggaaggac aaggaatttc
     3781 ctgttcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc
     3841 tcttcctgct tcctgatttc ttccaggttc gcctgtatcc tcccgccaag ataccagagc
     3901 gtcccgccac tcgcggtaaa caggagaaag actatcccca gtaacatcat gcccgtattc
     3961 cctgccagct ttaacacatc cttcctgtgc tgcatcatcg cctctttcac cccttcccgg
     4021 tgtttttcca gcgatttctc tgtcgaggct gtgaacaggg ctatagcgtc tctgattttc
     4081 gtctcgtttg atgtcacagc ctcgcttaca gattcgccga gcctcctgaa ctcgttgttc
     4141 agcattttct ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc
     4201 tgtaaaagat tgctcataaa atgctccttt cagcctgata ttcctcccgc cgttcgggtc
     4261 tgcaatgctg atactgcttc gcgtcaccct gaccacttca atccctgcct ccgtgagcgc
     4321 ctgaatcaca tcctgacggc cttttatctc cccgacatga taaagagcgt ctatcccgcg
     4381 tgtgacgctc tctgcaagcg cctgtttcgt ttccggcagg ttatcaggga gagtcagtat
     4441 ccggcggttc tccggggcgt tggggtcatg cagcccgtaa tggtgattaa ccagcgtctg
     4501 ccaggcatca attctcggcc tgtctgcccg gtcgtagtac ggctggaggc gttttccgct
     4561 ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc
     4621 cacccacagg atgctgtact gatttttttc gagaccgggc atcagtacac gctcaaagct
     4681 cgccatcact ttttcacgtc ctcccggcgg cagctccttc tccgcgaacg acagaacacc
     4741 tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatt
     4801 accctgaagc accgttgccc cttcccggtt acgctccctt cccagcaggt aatcaaccgg
     4861 accactgcca ccaccttttc ccctggcatg aaatttaact atcatcccgc gtcccctgtt
     4921 ccctgacggc cagacgcagc cggcgcagct catccccgat ggccatcagt gcggccacta
     4981 cctgaacccg gtcaccggaa gaccactgtc cgctgttcac cttcctcgct gtctgattca
     5041 ggttattccc gatagcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagtttcc
     5101 cggaacgggc aaccggctca cccaggcaga cccgccgcat ccatactgcc agttgtttac
     5161 cctcacagcg ttcaagtaac ctggcatgtt catcatcagt aacccgtatc gtgagcatcc
     5221 tctcgcgttt catcggtatc attaccccat gaacagaaat acccccttac acggaggcat
     5281 cagtgactaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     5341 taacgctgct ggagaagctc aacgaactgg acgcagatga acaggcagac atctgtgaat
     5401 cgcttcacga ccacgccgat gagctttacc gcagctgcct cgcacgtttc ggggatgacg
     5461 atgaaaacca ctgacacatg cagctcccgg agacggtcac agcttgtctg tgagcggatg
     5521 ctgggagcag acaagcccgt cagggcgcgt cagcaggttt tagcgggtgt
//
