LOCUS       NZ_CP115323             2113 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET18-565 plasmid pCUVET18-565.3, complete
            sequence.
ACCESSION   NZ_CP115323
VERSION     NZ_CP115323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339854
            Assembly: GCF_030995185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,663
            CDSs (with protein)               :: 4,663
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 150 of 225
            Pseudo Genes (internal stop)      :: 39 of 225
            Pseudo Genes (multiple problems)  :: 52 of 225
            Pseudo Genes (short protein)      :: 4 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115323.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-565"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET18-565.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-06-27"
                     /collected_by="Pattrarat Chanchaithong"
     gene            203..1168
                     /locus_tag="PCS83_RS25025"
                     /old_locus_tag="PCS83_24960"
     CDS             203..1168
                     /locus_tag="PCS83_RS25025"
                     /old_locus_tag="PCS83_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1172..1438
                     /locus_tag="PCS83_RS25030"
                     /old_locus_tag="PCS83_24965"
     CDS             1172..1438
                     /locus_tag="PCS83_RS25030"
                     /old_locus_tag="PCS83_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          597..828
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gagtgggttt tgacaggtca aagaaaatgg agcagaattg aggcgttttt aatcggcgtt
       61 ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca cctcagcggc aaactctggt
      121 gacatgtact ggctcgcaat gcacaggtac gtgatgaata taccacatca aatcacagcc
      181 tgcccagatc ggagcaggct taatgtcaga agataaattc ctttcggact acagcccccg
      241 tgatgcagtt tgggataccc agcgcacgct taccgattct gtcgggggta tctaccagac
      301 tgctgctgaa ttcgagcgct atgcactccg tatggcctcc tgtagcggtt tgttacgttt
      361 tggttggtct accatcatgg aaaccggaga aacgcgccta cggcttcgta gtgcgcaatt
      421 ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga accctcatgt ggcaagcccg
      481 tttttatcag gctctaccga aaatcgttgt ggattacccg tcttcccgat ggttgtttct
      541 gacgttaact gtcaggaact gcgagatagg tgaacttgga acagtcctta cagcaatgaa
      601 tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg ggtggatcag
      661 ggctacggag gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc actgtctgct
      721 gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt aagcacgaac gttgggtaga
      781 actctggcgc gattgcttgc gggtgaacta tgagccgaat atcgatattc gggcagtaaa
      841 aactaagaca ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg cggttgctga
      901 aacgctgaaa tactccgtta aaccggaaga tatggcaaac gatcctgagt ggtttcttga
      961 gctgacgcgg cagcttcaca agcgccgttt tatctcgacc ggtggggcgc taaaaaacgt
     1021 cctccagttg gatcgagaaa ccaatgagga tcttgtcatt gccgacgatg taggggatgg
     1081 cactgatgac gggaagcgga cggcgtttgt ctgggattca ggtaaacggc gttacaaacg
     1141 cgcccctgag aaggataaat cggattaacg tatgaatatt aatattgaat acctgaatgg
     1201 aaataagact attggtttat tttttttaag aagtgaagcg gtgattcctg acaggtttaa
     1261 aaaccttatt ttgcttattg atggattaag ttttggcaca tttggttttc atccgcacga
     1321 aggttttgag gatgaattaa ttttatatat tcagaaaaca aacgagaggg taaaaactct
     1381 ttttgtgaaa atagatttaa acggtatcaa gtttggattt ttaagaacgc attcttagtt
     1441 ctggaaaaga gccagcggca ggctgaggtg ataggtacga gattgcatgc aatctctagt
     1501 gctctgtcta tcctgcatta tcctcagcat tatcctcagc attatcctca gcattatcct
     1561 cagccttgcc aactcgacac caatgcagga tagacaatcc gatgtcaaat gttaacactc
     1621 tgcgagtggt acattttccc cggattatcg tcctgagcct gccgctggct ctctttctac
     1681 cgcctcgctt tgctcgttgc tcaacgcctc acagacacgg attaaaatcc gcatccgttc
     1741 accgtttttt aaagtccgtt aaaagcatga tgccatctcc gagagttaat ctcgtcaaat
     1801 gctaaatcgt gggggtcccc tttggggttc cgatttagtg attgacgaca ccaccgatta
     1861 aaaaacttat gcggggtgga tggtttcacg aagtgaggcc atccacctgt aagacagggt
     1921 tttgttttta ttccctgttt tggtgatcgg gtgtgtggaa aaggttgggg taagccgttc
     1981 gggggtgctt gttttggggg gttaaaattg tggttatttt ttgcgcaatt ctcgcgcgtg
     2041 atccttgtat ttatacttaa gggataaatg gcggatatga aatagtggtt tagcccagta
     2101 atgacgaggc ttt
//
