LOCUS       NZ_CP115322             4548 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET18-565 plasmid pCUVET18-565.2, complete
            sequence.
ACCESSION   NZ_CP115322
VERSION     NZ_CP115322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339854
            Assembly: GCF_030995185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 16:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,006
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,663
            CDSs (with protein)               :: 4,663
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 150 of 225
            Pseudo Genes (internal stop)      :: 39 of 225
            Pseudo Genes (multiple problems)  :: 52 of 225
            Pseudo Genes (short protein)      :: 4 of 225
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-565"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET18-565.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-06-27"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(71..1039)
                     /gene="mobV"
                     /locus_tag="PCS83_RS25005"
                     /old_locus_tag="PCS83_24940"
     CDS             complement(71..1039)
                     /gene="mobV"
                     /locus_tag="PCS83_RS25005"
                     /old_locus_tag="PCS83_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852935.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053272216.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADADRTPENHCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     NKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSQAVNDGFAGTIAMASQSAQNAKRARDLQKTMDSQQKRLQSVTEPFKGLSREQM
                     THILTMAQTFQQQNHDREKQRQLEREQERQQRQKTDRGISR"
                     /name="mobV"
     gene            1635..2156
                     /locus_tag="PCS83_RS25010"
                     /old_locus_tag="PCS83_24945"
     CDS             1635..2156
                     /locus_tag="PCS83_RS25010"
                     /old_locus_tag="PCS83_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657475.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_099368844.1"
                     /translation="MDNSQIGTPESHEQDNYNFVQVSRAYLKEWRALTRKSPIASEILM
                     YLIENMGRTTNAVVCSYTTLMEITGVSRASVARAVKILKEDRWMQAVKIGNATAYCVNA
                     KAFWQAGRNQKKYAIFQATVIASSSEQESDFLEKSQQDLRYIPFVDLKDRPIVGTDELP
                     PPDQTDLDLD"
                     /name="replication/maintenance protein RepL"
     gene            2502..3212
                     /locus_tag="PCS83_RS25015"
                     /old_locus_tag="PCS83_24950"
     CDS             2502..3212
                     /locus_tag="PCS83_RS25015"
                     /old_locus_tag="PCS83_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053272217.1"
                     /translation="MLYWKKMPSLWIGNRLTEFNSLETSHAIAAIKIYITFCLFGKEED
                     DGVRTVKLTFTEISEIASISRSLVNEGLKLLDNKKLIKYLSPASKRKKIYTVDVDGCIH
                     DGWCKLPMAGIVSEDRTISAFQSMHNRYHFELVALQLYLYLLYARDNFQEHVLARKQTI
                     CEKLACRNVDLNKAITYLIHIGLLKGIKQKAVESYPIELFHDSFYFYVKTGASNALTFK
                     KQPLVNPPEKEIPY"
                     /name="hypothetical protein"
     gene            complement(3500..4480)
                     /locus_tag="PCS83_RS25020"
                     /old_locus_tag="PCS83_24955"
     CDS             complement(3500..4480)
                     /locus_tag="PCS83_RS25020"
                     /old_locus_tag="PCS83_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="WP_213091166.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELTEVDVDWLIAERPGKVRTLKQHPRKNKTAINIEYMKASIRAKVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
                     /name="IS5-like element IS5 family transposase"
     Typing          complement(177..1069)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.289"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctaggatcca ttccctcaag tcttagtgat gcttccgctt ttcgaaaccc ttcacggcgc
       61 tgcttttcac tcatcggcta atacctctgt cagttttctg acgttgctgg cgttcctgct
      121 cccgttcaag ctggcgctgt ttttcacgat cgtgattctg ttgctggaac gtctgcgcca
      181 tcgtcagaat atgggtcatc tgctcccggg aaagtccttt aaaaggctct gtaacgctct
      241 gtaggcgttt ttgctgcgaa tccatagtct tctgtagatc gcgcgctctt ttggcattct
      301 gggcgctctg agaggccata gcgatggttc ctgcaaagcc atcgttcact gcctggctta
      361 aacgtttggc tatggctgcc tgatcttcca cgtctttggt gaaaataccc ttccgcagta
      421 cacgcggctc cagcgcctcc ggtgagatcg atacctggct acgggtgcca cggtttatcg
      481 attcgtagta gtgctgaact gtctgatgcg tggcacggct tccctcgatg ccgcgctcaa
      541 gacccagttt ttttaccgat tcagcgtagg tgctctgatc gtcacgcatc tttgaccgcc
      601 cgccaataaa ctctttcgcg gacagccgcc catcctgcgt caatggcacc acgaacgcag
      661 acaggtgcgg ggtggcctcg tccctgtgaa ccaccgccgc cacaacgcga tctttcccgt
      721 atttgttctc cagccattgc tcagaacggg cgaaaaactc cgcctgctgc cgcggtgtgg
      781 cctcgttcca ccactcaggg cttgctgtca taacgtattc aacagccagt accgcatctt
      841 tacggcgttt ttccggcaac agctcccgca gctttcccat cgcctcatct gccgactgag
      901 acacagagca gtggttttct ggtgtccggt cagcatcagc attcggcgtt tctctctccc
      961 tgtagcagtg ctgtaacgcg ctggccacac tgcccattcc cgtcagcttt ttacagcgca
     1021 ttatcgcgta gttcgccatg cttccccccc ccctcgttcc ggcgtccatc cggccagacg
     1081 ggggatcgca ttgcgatgcc tccggcgggc cactatcagg atgatagtgt actcactaga
     1141 ctttgctacg caaaatcgtt cgggcagcaa gctgccagaa ggtcaaaatc aacggcagtc
     1201 tggcaggtgg aaggcgtcgc tccgcgccac cccccaccac tgccaagcct gttttatccg
     1261 gtgagtcgtc aagggtaaat ggcactcgct acgctcgccc ttgacgaatg tcaggcaaca
     1321 aagtcgtctt tgaaagagca cgtgccgctc gccgcaggtc aggctgaggc gtaccgcagc
     1381 gcagcgagga acgaacagcc tgaacgagga agcggaatgt ccactaggcg tacattgggc
     1441 tacgtatgca cggacttaat agatatagaa aaggagcttt cagtaaatcg ctgattatat
     1501 tgggttaaaa aaagggggag tctcaccagc catgagactt atgagtctca ctagctatga
     1561 gactcaagtt gacctgttgc atctctaatg atacaaccgg tatcaaatgg gatattgaaa
     1621 tggtctatag agcaatggat aattcacaga ttggcactcc agagtctcat gagcaggata
     1681 actataattt cgtccaggtt agccgcgcgt atctcaagga atggcgtgcg ctgacaagga
     1741 aaagccctat tgcctctgaa atccttatgt acttgattga gaatatgggc aggacaacta
     1801 atgctgttgt ttgcagctat acgactttga tggagataac cggggtctct agagcatcag
     1861 tagcaagggc ggttaaaatc ctgaaggaag atcgatggat gcaggcagta aaaataggta
     1921 atgccacagc atattgtgtt aatgccaaag cattttggca ggcaggtaga aatcaaaaaa
     1981 aatacgcaat cttccaagct accgtgattg cctcctcctc agagcaagag tctgatttct
     2041 tagaaaaatc acagcaagat cttcgatata ttccttttgt cgatctcaaa gaccgcccta
     2101 ttgttggcac cgatgaactc ccgcctccgg atcaaacaga tctagatctc gactgagcct
     2161 ctgaaaacct tcaaatatgg ttcaccgtga accatattga agttcatttg ataaccattt
     2221 ttttaagtta actttttgat ttaattataa aaattcatat taattttttc ccataccttt
     2281 ctagatagat ttttgctcct atagatacag atacgcggat tactgggata atcggtatat
     2341 cggtaaacag gagcagaggt caaaatgggt gacttagagc gatttgtaga gcttgctgaa
     2401 aggctgggaa aagtggcagg gtcacttact agtcttgcat tggccatttg tgtgttaatt
     2461 atgatttatt tcactttcat ttccgcataa aggaatcact aatgttatat tggaaaaaaa
     2521 tgccatcact ctggatcggt aatagactga cagagtttaa tagtttagag acttcacatg
     2581 ccattgccgc cataaagata tatataactt tttgtctctt tggaaaagaa gaggatgatg
     2641 gagtgcgaac cgtaaaactt acgtttacag aaataagcga aatagcttct atcagccgat
     2701 ctttggtaaa tgaaggtctc aaattgctcg ataataaaaa actaattaaa tacctatcgc
     2761 ccgcgtcaaa aagaaagaaa atctatacag tggatgttga tgggtgcatt catgacggat
     2821 ggtgtaagct gcctatggca ggcatcgtta gcgaagacag aacaatcagc gccttccagt
     2881 caatgcataa tagataccat tttgagcttg tagcgcttca gctatattta taccttctat
     2941 atgccagaga taacttccag gagcatgtgt tagctagaaa acaaactatt tgcgaaaagc
     3001 ttgcttgccg caatgtagat ttaaataaag caataacata tttaatacac ataggtcttt
     3061 taaaaggaat taaacaaaag gcagtggagt cttatcccat agaacttttt catgattcat
     3121 tttattttta tgtaaaaaca ggagcaagca atgcgcttac attcaaaaag caacctcttg
     3181 ttaaccctcc agaaaaagaa attccgtact gacatacctg agggcgaatt actcctcgcc
     3241 cttatgattt attctaaggt agtaatccaa tatttcttta cgcccttgtt cataacttgt
     3301 ttcgccagag ataatcctcg ttttcaaaca ttcataacga ggtactccac tagggaaggt
     3361 gcgaataagc ggggaaatac ttctcggctg actcagtcat ttcatttctt catgtttgag
     3421 ccgatttttt ctcccgtaaa tgccttgaat cagcctattt agaccgtttc ttcgccattt
     3481 aaggcgttat ccccagtttt tagtgagatc tctcccactg acgtatcatt tggtccgccc
     3541 gaaacaggtt ggccagcgtg aataacatcg ccagttggtt atcgtttttc agcaacccct
     3601 tgtatctggc tttcacgaag ccgaactgtc gcttgatgat gcgaaatggg tgctccacct
     3661 tggcccggat gctggctttc atgtattcga tgttgatggc cgttttgttc ttgcgtggat
     3721 gctgtttcaa ggttcttacc ttgccggggc gctcggcgat cagccagtcc acatccacct
     3781 cggtcagctc ctcgcgctgt ggcgcccctt ggtagccggc atcggctgag acaaattgct
     3841 cctctccatg cagcagatta cccagctgat tgaggtcatg ctcgttggcc gcggtggtga
     3901 ctaggctgtg ggtcaggcca ctcttggcat cgacaccaat gtgggccttc atgccaaagt
     3961 gccactgatt gcctttcttg gtctgatgca tctccggatc gcgttgctgc tctttgttct
     4021 tggtcgagct gggtgcctca atgatggtgg catcgaccaa ggtgccttga gtcatcatga
     4081 cgcctgcttc ggccagccag cgattgatgg tcttgaacaa ttggcgggcc agttgatgct
     4141 gctccagcag gtggcggaaa ttcatgatgg tggtgcggtc cggcaaggcg ctatccaggg
     4201 ataaccgggc aaacagacgc atggaggcga tttcgtacag agcatcttcc atcgcgccat
     4261 cgctcaggtt gtaccaatgc tgcatgcagt gaatgcgtag catggtttcc agcggataag
     4321 gtcgccggcc attaccagcc ttggggtaaa acggctcgat gacttccacc atgttttgcc
     4381 atggcagaat ctgctccatg cgggacaaga aaatctcttt tctggtctga cggcgcttac
     4441 tgctgaattc actgtcggcg aaggtaagtt gatgactcat gatgaaccct gttccatggc
     4501 tccagatgac aaacatgatc tcatatcagg gacttgttcg caccttcc
//
