LOCUS       NZ_CP115319             2464 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET18-789 plasmid pCUVET18-789.7, complete
            sequence.
ACCESSION   NZ_CP115319
VERSION     NZ_CP115319.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339855
            Assembly: GCF_030995165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:01:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,748
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 66 of 232
            Pseudo Genes (incomplete)         :: 163 of 232
            Pseudo Genes (internal stop)      :: 45 of 232
            Pseudo Genes (multiple problems)  :: 40 of 232
            Pseudo Genes (short protein)      :: 3 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115319.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-789"
                     /isolation_source="urine"
                     /host="cat"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET18-789.7"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-05-30"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(join(2316..2464,1..82))
                     /locus_tag="PCS95_RS24320"
                     /old_locus_tag="PCS95_24275"
     CDS             complement(join(2316..2464,1..82))
                     /locus_tag="PCS95_RS24320"
                     /old_locus_tag="PCS95_24275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029488008.1"
                     /translation="MMNAEDKLFKKVDRLMLHDSLKKNEVLTVWERTFLSSTYSIYRRT
                     SGNEFSTKGLSPKQKSTAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     gene            complement(69..551)
                     /locus_tag="PCS95_RS24325"
                     /old_locus_tag="PCS95_24280"
     CDS             complement(69..551)
                     /locus_tag="PCS95_RS24325"
                     /old_locus_tag="PCS95_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202974839.1"
                     /translation="MNTKKISKLERHTYMDESGEIKQDSKIETFSIPAEPPYVKLYIAD
                     IAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVD
                     KQIFLRAGRGELEANPFLFARGDWPSIYERRKKIELRISYDSNGRTVQTTVTDDER"
                     /name="hypothetical protein"
     gene            1249..2217
                     /gene="mobV"
                     /locus_tag="PCS95_RS24330"
                     /old_locus_tag="PCS95_24285"
     CDS             1249..2217
                     /gene="mobV"
                     /locus_tag="PCS95_RS24330"
                     /old_locus_tag="PCS95_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000024818.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     Typing          1241..2214
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 tcaaaacctc attctttttt agagagtcat gcagcattaa tctatctact tttttgaata
       61 gcttatcttc agcgttcatc atccgttacc gttgtttgta ctgtccttcc attggaatca
      121 taactgattc ttaactctat tttttttctt ctttcgtaaa ttgaaggcca gtctccccga
      181 gcaaaaagaa acggattggc ttcaagctct ccccgccctg cccggagaaa gatttgttta
      241 tcaacaaggg cagcgattat gtttttaaac gtctgatatt caatatgaac cctaggttgt
      301 atggtcttct caaatctagt ttttgataca gatacaaagc catcccatcc aatcagcatt
      361 gccaactgca aaagcacatc attttgagat tttgacaaac catgaatcat cgcaatgtct
      421 gcgatgtaaa gcttgacgta gggcggttca gccgggatag aaaaagtctc gatcttagag
      481 tcttgtttaa tctctccgct ctcgtccatg tatgtatgcc gttcaagctt agaaatcttt
      541 tttgtattca ctctatcccc atgtatcagc atgtttttag tactcacatt taagtactaa
      601 agatgccggt agtcaagtac cttacctaaa gtacgattag tacttgaggt gagtacgatt
      661 agtacttgag gtaagtacga ttagtacttg aggtaagtac gattagtact tgaggtgagt
      721 acgattcttc tctctagccc ttggtgtgcg cgggttcccg tatctatatc tatatattaa
      781 gggggaaaat aaaggctgaa aaacgccatt tttaaacatc gaaaaacgag cttttttgag
      841 tctggagcgt acggatttct atgtccgggg agtggaaatt ccgcttcctc gaacggacag
      901 actcgcggcg atgccgctcc ctaatccgtt cttgcggcga gcggttcctg ctctttcaaa
      961 aacgatattc attgcctgat ttcgtcaagg gcgagcgtag cgagtgccat ttacccttga
     1021 cgactcaccg gataaaacag gcttggcagt ggtgggaggt gacgcggagc gacgccttcc
     1081 acctgccaga ccgccgttga ttttgacctt tggaagcgca gcttcccgaa cgattttgcg
     1141 aagcaaagtc tagtgagtac atacactatc atcttgatag tggcccgccg gaggtatcgc
     1201 aatgcgctcc cccgcctggc tgaatgaatg ccggaatcag gggggaacat ggcgaactac
     1261 gcgataatgc gctgcaaaaa gctgacggga atgggaagcg tggccagcgc gttgcagcac
     1321 tgctaccgag agcgtgagac gccgaacgct aacgctgaac ggacaccaga aaactactgc
     1381 tctgtgtcca aatcaacgga tcaggcaatg gggcgtgtgc gggagctgct gccggaaaaa
     1441 cgccgtaaag atgcggtact ggccgttgaa tacgttatga cagcaagccc tgagtggtgg
     1501 aaagaggcca caccacggca gcaggcggag tttttcgccc gctctgagca atggctggag
     1561 aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc accgggacga ggccaccccg
     1621 cacctgtctg cgttcgtggt gccattgacg caggatggac ggctgtccgc gaaagagttt
     1681 attggcgggc ggtcaaagat gcgtgaagat cagagcacct acgctgaatc agtaaaaaaa
     1741 ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc atcagacagt tcagcactac
     1801 tacgaatcga taaaccgtgg cacccgtagc caggtatcga tctcgccgga ggcgctggag
     1861 ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg aagatcaggc agccatagcc
     1921 aaacgtctaa gccacgcagt gaacgatggc tttgcaggaa ccatcgctat ggcctctcag
     1981 agcgcccaga acgcaaaaag agcgcgtgag ctacagaaga ctatggatgc gcagcagaaa
     2041 cgcctacaga gcgttacaga gccttttaag gggctttcca gagagcagat gaccgaaatc
     2101 ctgatgatgg ctcagcggtt taagcagcaa aaccaggaaa aggaaaaaca gcagcgtatt
     2161 gaacgtgaaa aacagcgaca gacgcgatcg cgtggcatgg gtggtatgga gcgttagttt
     2221 tgatgcgatg caggataggg ctaacgccgc aaagtgacgt catttgcggc attccagaga
     2281 tcaggttctg ccgccctgta taacgctgag tccagctaat ccatcagatg gtaattgtat
     2341 tttttcaaaa tagaaaaagc ggtgcttttt tgttttggtg acaatccctt agttgaaaat
     2401 tcattaccgc ttgttctgcg atatatgctg tatgtactac ttaaaaaagt acgctcccaa
     2461 acag
//
