LOCUS       NZ_CP115316            10121 bp    DNA     circular CON 03-SEP-2023
DEFINITION  Escherichia coli strain CUVET18-789 plasmid pCUVET18-789.4, complete
            sequence.
ACCESSION   NZ_CP115316
VERSION     NZ_CP115316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32339855
            Assembly: GCF_030995165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/31/2023 17:01:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,866
            CDSs (total)                      :: 4,748
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 66 of 232
            Pseudo Genes (incomplete)         :: 163 of 232
            Pseudo Genes (internal stop)      :: 45 of 232
            Pseudo Genes (multiple problems)  :: 40 of 232
            Pseudo Genes (short protein)      :: 3 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-789"
                     /isolation_source="urine"
                     /host="cat"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET18-789.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-05-30"
                     /collected_by="Pattrarat Chanchaithong"
     gene            <1..339
                     /locus_tag="PCS95_RS24165"
                     /old_locus_tag="PCS95_24120"
                     /pseudo=""
     CDS             <1..339
                     /locus_tag="PCS95_RS24165"
                     /old_locus_tag="PCS95_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241261.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]; GO:0043565 - sequence-specific DNA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(353..787)
                     /locus_tag="PCS95_RS24170"
                     /old_locus_tag="PCS95_24125"
     CDS             complement(353..787)
                     /locus_tag="PCS95_RS24170"
                     /old_locus_tag="PCS95_24125"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273090.1"
                     /translation="MIALSIIRLLHYMEQTPDTYSYTACHIGCSSVLVEDSSTPARATN
                     SGLENTKKPDYQSGFFVPVVRLFFRFFPSFGGFFFHFLLRPILLFALANCVMALLTFKS
                     IVRNNTVQNIYIRACTEVSLTINEHLDQNLLAEIIGKIRK"
                     /name="hypothetical protein"
     gene            768..851
                     /gene="dinQ"
                     /locus_tag="PCS95_RS24175"
                     /old_locus_tag="PCS95_24130"
     CDS             768..851
                     /gene="dinQ"
                     /locus_tag="PCS95_RS24175"
                     /old_locus_tag="PCS95_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="WP_000565612.1"
                     /translation="MIDKAIIVLGALISLLELIRYLLQLMG"
                     /name="dinQ"
     gene            complement(1006..1650)
                     /locus_tag="PCS95_RS24180"
                     /old_locus_tag="PCS95_24135"
     CDS             complement(1006..1650)
                     /locus_tag="PCS95_RS24180"
                     /old_locus_tag="PCS95_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203396.1"
                     /translation="MTSNVPTSFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGTRLERPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKAKKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISNALKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            2031..2693
                     /locus_tag="PCS95_RS24185"
                     /old_locus_tag="PCS95_24140"
     CDS             2031..2693
                     /locus_tag="PCS95_RS24185"
                     /old_locus_tag="PCS95_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            2791..3072
                     /locus_tag="PCS95_RS24190"
                     /old_locus_tag="PCS95_24145"
     CDS             2791..3072
                     /locus_tag="PCS95_RS24190"
                     /old_locus_tag="PCS95_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            3097..4074
                     /locus_tag="PCS95_RS24195"
                     /old_locus_tag="PCS95_24150"
     CDS             3097..4074
                     /locus_tag="PCS95_RS24195"
                     /old_locus_tag="PCS95_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_024245156.1"
                     /translation="MRYLTELICDLYRLTDHHKLTTATFKNLADIKLFKPTADADTILQ
                     LENIFSEYSLRYIDKDLAEILSNLITTDKKHSLDFDFNKIQSLTDSKSFGCGWSKVING
                     SWFKDLYSWGEGMYPAENLKAENILDWKDNIWHIEHEGFPPRPPINVKYYSWFDRYVAS
                     NSGGSHHAAMVVYQSVRDGLEYKREAVIKQLSIDPGTVEILDKNYYSFIFQIKTLNKET
                     RIYTSEYEFTDALKDFGQNRHTILKPVNYVSNIKLVFIPKDALKTNSETFRNWFHSAIS
                     CGKIISLPDYLKNPAHYHTLHYSHEVDSITLGDPSRKFKLKGDS"
                     /name="DUF6685 family protein"
     gene            complement(4071..4427)
                     /gene="ddp1"
                     /locus_tag="PCS95_RS24200"
                     /old_locus_tag="PCS95_24155"
     CDS             complement(4071..4427)
                     /gene="ddp1"
                     /locus_tag="PCS95_RS24200"
                     /old_locus_tag="PCS95_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000456533.1"
                     /translation="MEYQVREFINEKYTKAVNILKDNLKENYHVFYGVRLIEILFPASE
                     YGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISFDKILDASLLEGTNIVVLECITLA
                     DLLTNDNIDFLYKS"
                     /name="ddp1"
     gene            complement(4564..5010)
                     /locus_tag="PCS95_RS24205"
                     /old_locus_tag="PCS95_24160"
                     /pseudo=""
     CDS             complement(4564..5010)
                     /locus_tag="PCS95_RS24205"
                     /old_locus_tag="PCS95_24160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5050..5886)
                     /locus_tag="PCS95_RS24210"
                     /old_locus_tag="PCS95_24165"
     CDS             complement(5050..5886)
                     /locus_tag="PCS95_RS24210"
                     /old_locus_tag="PCS95_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050932.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_001050931.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDIRIPYGFRG"
                     /name="RepB family plasmid replication initiator protein"
     gene            7120..7371
                     /locus_tag="PCS95_RS24215"
                     /old_locus_tag="PCS95_24170"
     CDS             7120..7371
                     /locus_tag="PCS95_RS24215"
                     /old_locus_tag="PCS95_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            7361..7642
                     /locus_tag="PCS95_RS24220"
                     /old_locus_tag="PCS95_24175"
     CDS             7361..7642
                     /locus_tag="PCS95_RS24220"
                     /old_locus_tag="PCS95_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7717..8414
                     /locus_tag="PCS95_RS24225"
                     /old_locus_tag="PCS95_24180"
     CDS             join(7717..7966,7966..8414)
                     /locus_tag="PCS95_RS24225"
                     /old_locus_tag="PCS95_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            8716..8910
                     /locus_tag="PCS95_RS24230"
                     /old_locus_tag="PCS95_24185"
     CDS             8716..8910
                     /locus_tag="PCS95_RS24230"
                     /old_locus_tag="PCS95_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053273080.1"
                     /translation="MEKIGNDIPHLIDQAIDFMASTQAFREYLKKLPPRNTIPSDIPEE
                     NVPLYLQRLEYYRQLYRPK"
                     /name="hypothetical protein"
     gene            complement(9102..>9353)
                     /gene="tnpA"
                     /locus_tag="PCS95_RS24235"
                     /old_locus_tag="PCS95_24190"
                     /pseudo=""
     CDS             complement(9102..>9353)
                     /gene="tnpA"
                     /locus_tag="PCS95_RS24235"
                     /old_locus_tag="PCS95_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525033.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /name="tnpA"
     gene            9409..10106
                     /locus_tag="PCS95_RS24240"
                     /old_locus_tag="PCS95_24195"
     CDS             join(9409..9658,9658..10106)
                     /locus_tag="PCS95_RS24240"
                     /old_locus_tag="PCS95_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_103215986.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     Typing          complement(4565..5010)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.21"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcaaacttaa gagcattcct cacatcaggc atgcctacgt tttcaggaaa agttcgaagc
       61 attcttcttt cagatgtttc taaacactgg aaattgagag atttagctga ttatctttac
      121 atgagcgaga gcttaataaa gaaaaagcta ttattagaaa acacttcttt cagtaaaata
      181 ttacttgata ccaggatgtc gcttgccatt aaattgttaa agcaaaatca tccgataaaa
      241 tatgtgtctg aaatgtgtgg gtttagtagt acatcgtact ttgtatgcct tttcagacaa
      301 tactatgagt gtacaccacg taaatatcta aagcattaga gaacacctgc ctttacttcc
      361 ttattttacc aataatttca gctaagagat tttgatctaa atgttcgtta atcgttaagc
      421 ttacttctgt acatgccctt atatagatgt tctgaacggt gttatttctg acaatggact
      481 taaatgtcag taacgccatt acacagttgg ccaacgcgaa cagcagtatt ggtcgcaaca
      541 aaaaatgaaa gaaaaatccc ccaaatgaag ggaaaaaacg gaagaacagt cgaacgacag
      601 gcacaaaaaa acccgactgg taatcgggct ttttcgtgtt ttccagtcct gagttggtgg
      661 ctctggctgg agtggaactg tcttcaacca acaccgaaga acaaccgata tgacaggcag
      721 tgtaagagta agtatcggga gtctgctcca tataatggag taaacggatg attgataaag
      781 caatcatcgt tctgggggca ctaatctcac ttcttgaatt gattcgctac cttctccagt
      841 tgatgggctg atggcggaaa cgtaactaag gccgggggct aaccacctcc ggcctttaat
      901 aaaaacatat tttagttaca atattcaaat gcattgacta aacaccacac acccaatctc
      961 ctgagttaat atcctgcctt gcaggtatat tagcccccta ctcctctact tactggattt
     1021 ctgcccggtt ttacgtttta gcgcattact gattgtgcca cggctgcacc ctagcacttt
     1081 ctgtacctgt gtccaggagc taccgctttc tatcaacctg ataattgcat catgtctggc
     1141 ttgattaggt ttacgccctt tatatttgcc gtctttcttt gctttctcta tcccttgttt
     1201 ctggcgttcc cgacgttgtt catagtccct tctggcaact gcggccagca tatccagcag
     1261 catatcgtta atggctgcaa acaaccggct gtcaaactca ctcataccag aattaatcca
     1321 tgtcgtcggc acattcaccg ccataacccg gatgtctttc tgccggatca ttttcttcaa
     1381 cgtattccag tcttcccctg acaggcggga aagtctgtcc acatcctcga taagcaaaat
     1441 gtcattttgt tggcaatctc tcagaagacg aaaaagttcc gggcgctcaa gacgggtgcc
     1501 ggattcattc tcaatatagt agctgcaaat gatcaggcct ctttccctgg caaaagcctc
     1561 gatagtttcc agggcacgcg aagcatcctg ttctgctgtt gaagctctca gataagcgcg
     1621 gacaaagctt gttggtacat ttgaagtcat attacagaac cggttcacat aaggttacac
     1681 cactataccc aaaatgcacc agaaagtaga tcgacaggaa aggcacactc aaaaagaacc
     1741 agaaaacaga cctccgacca taaagtgaca atgtcacaca gaaatttaca acataaagat
     1801 gatgtaaaga cggtctcaga tttaccacgt aaagccacta taaatacaca caaatcttta
     1861 cacaataaag ataacataaa tacaagctta tctttatttg tgctttacat ggtaaatcat
     1921 gtgtaatata aagacactgt aaagacaaaa taatctttac gaacaacaca caatgtaaag
     1981 ataagacagt ctttacataa tctttacgtc ataaagagag gaattctgaa atgggtaaaa
     2041 tcttgcttgt cgtatcagat aagggtggtg taggcaaaag cacctacgtg gctaacacag
     2101 gctcaatgct ggtcaataaa ggtaagtcgg taattatcct caagacagat aaaaaccacg
     2161 atctgttgag ctggaacgaa aagcggacag ataacggctt actcactatc ccggttcacg
     2221 cagcttacgg aaacgtcagc aatgaaatta agcgacttag taagctgtgt gaagtcctca
     2281 ttgtcgattg ccccggccac gacagccagg agttccgtag cgccctgaca gtatcagata
     2341 tcgtggtaac actggttaag ccgtcctctg atttcgaaag cgaaactctg accagtgtta
     2401 cagagaaaat ccgcaccgct cagaaagcta acgcagccct ggaaccgtgg gtgctgttca
     2461 ccagaatcaa ctcagcgaaa ccccgccaca gaaaagcggc cattgatctg gataaattac
     2521 tgcgttcgga caacatctgg attcaacctc ttaaaacccg gatatccgag ctggatgtgt
     2581 atgaaagcgc ctgtaatgaa ggggcagggg tacatgacgt tagccgggca tcaagccttt
     2641 caacagcgaa agcacagatt gaactagtgg cgcaggaaat tggaatttta tagtaatatc
     2701 aataccatct ataaatcttt acattgtaaa gataatataa agatagtatg atatttacta
     2761 ttaatgcata ccgtacaagg ggaaattgtc atggctttaa aactaaaccg tccgaacatc
     2821 gatgaatctc agcaaccagc aacaaacgca gaaaccgcgc gttttatctc cggcgctacc
     2881 agagcgcctg tagtgggtaa accaaagttg atgaacttca gactttcagc ccattttgag
     2941 gacatactgg aagctgaagc tatcagaaca gggcagagca aaacaaccgt tctcaaagcg
     3001 gcgctggcga tgtacaacag ccaggatgaa aacataaaga atcactggtt gcttgagtct
     3061 gcaaaattct aaccatagaa gcatggatga aaatctatga gatatttaac cgaattgatc
     3121 tgtgacctct acaggttgac agatcaccat aaactgacca cagcaacgtt taaaaatctt
     3181 gctgatatta agttatttaa acctacagca gatgcagaca ccatattgca gcttgaaaat
     3241 atatttagcg aatactcact gagatatatt gacaaggatc tggctgaaat tctgagcaac
     3301 ttaataacta cagataagaa acactctctg gacttcgatt ttaacaaaat acaatctctg
     3361 actgactcga aaagtttcgg ttgtggatgg agcaaggtaa taaatggtag ttggttcaaa
     3421 gatctttatt cttggggaga ggggatgtat cctgccgaaa atctgaaagc tgaaaatatt
     3481 ttagactgga aagataatat atggcacatt gagcatgaag gtttcccccc acgcccccct
     3541 attaatgtta aatattattc ttggtttgac cgatatgtag cttcaaacag tggtggctca
     3601 catcatgcag caatggttgt atatcaatca gtcagagacg ggttagagta taaaagagaa
     3661 gctgttatta aacaactgag tatagaccca ggcactgttg aaatacttga taagaattac
     3721 tattcattca tattccagat caaaaccctt aataaagaaa ctagaattta tacatctgaa
     3781 tacgagttta cagatgcttt aaaagacttt ggacaaaaca gacataccat acttaaacct
     3841 gttaattacg tttccaacat aaaattagta tttattccaa aagatgcatt aaaaacaaat
     3901 agcgagacat tccgcaactg gtttcattct gcaattagct gcggaaaaat aatatcactc
     3961 cctgattatc tgaaaaatcc agcacattat catacacttc attactcaca tgaagtagac
     4021 tcaataacac ttggcgaccc aagcagaaaa tttaaactga aaggagattc ttagctttta
     4081 tataaaaaat caatgttatc atttgtcaga agatcagcga gggtaataca ttcaagaacc
     4141 acaatatttg ttccttccaa caaactggca tcaagtatct tatcgaagct gataatcatc
     4201 attggcttgc gttgtgttaa atcaaagatt accaatggaa gtattacact gttaattaat
     4261 tcaaactctt taaagaaagc atcagtgcca tactcactgg ctggaaaaag aatctcaatt
     4321 aatctcacac cataaaaaac atggtagttt tctttaaggt tatcctttaa aatattaaca
     4381 gccttagtat atttttcgtt aataaactct ctcacctgat attccataaa attctccgac
     4441 aagaaccgac tgtaggtcac cgggcaaacg ttgcagaatg gcgtcagaga cgtcattttg
     4501 cggcgtttgc cctatcctgc atcgcagtga aaaggcaggg actggcataa ctgctgagta
     4561 gcatcattgc tggcttatct cgctactgtt ctcggtgctt tcgtccgcag gttcgggaac
     4621 aagtaacttg tgccaaggca tagttttgtt gagcagctcc atcagaggct cagccagcag
     4681 tcccaaaatc caggttgccg taatcaccgg tatagtcaga ttttgtggcg tccataactg
     4741 gtgatcgaga tgataccaag tgatgacaca caaaatacca cccagccaga atacagccca
     4801 tttaaacgca ccaagaatgg ctaacacaaa aacaaagata aaatgtaaaa cactcgcgac
     4861 aagaagacga agataaaacc atcctcagcc aagccactta acaaaagtgc tccctgtttt
     4921 cactgcaaaa cgtttcttac gctccctggc aatttctttc ctgaatttag cacgttcttc
     4981 cccctgaggg aattgataga ttttagccat gagctacctc atttcgatct caaaacgaag
     5041 tttatgcaat tatcctctga aaccgtatgg tattctgata tctaagccct cactttcttt
     5101 atctatttta taagaaaacc ttagctttga tgctcttctt gcgactctct caacgatttt
     5161 aacttcgaga tgattaatct ctgtatattg atttattttt tcaatatttt tttgcagaaa
     5221 aaccttatta aaatccttaa aattctgata aaggtaaact ttttcgccat tatctattct
     5281 gaataattca agttcatcct ttaatacatc tacttcaatg tcgaaaaatg tctttttacc
     5341 actgctgtat tgtttcctga taaactgata aagcatattt gaattttgat cagcgagtct
     5401 tacagacgat atcaaaacct gagtagtgta agaatccttt aacatcgtga tatatggctc
     5461 aatgacatgt gaaaaagcaa tatcaatata tccttcactt tcaacataat cacaaaacgc
     5521 agtcaggttc ataagcctga ctgcatcaga aggtaattta cctctcttct tcgtatgtcc
     5581 agagctttga ttgccctgag gtgtgagaag ttgatttcga ggaatgcgaa taacactggc
     5641 ctgaagttct tctgccccct ctttcaactg ccggtaagca cctgttacat caatatcagc
     5701 gataaatgca tattcccgag ccgtaatacg aaaggttgcc ccctcagata actcttcacg
     5761 tgaatcaatc tgcgccatag ccataaacaa aattcttctg gcagacaaag gcaacgatga
     5821 gaatgtgctg ttaatttcat ttctgtggcg aatttttgtt ttctttgtaa cgctcagtaa
     5881 gttcatacat tacctgtggt ttttaagttc tgatggtgaa atcataacca caaaggcgga
     5941 aatatgtaaa gtggtttatc caccttatgt atttccacca aaacacggat cacatgaaag
     6001 aatttttatc atttatattc aatatgatag aaatgcgcag gtatccacct tttaacgtca
     6061 tacgttaaat tagagcacta taaagaacta aagaaatgaa gatacaaaga tacgaatatc
     6121 tgaataaaaa cagaaaaatg cgtacccatc cacctttcag tgcgtaccca tccacctttc
     6181 agtgcgcagc catccacctt tcatgaaaat tacagtgcgt acccatccac ctttcagtgc
     6241 gtacccatcc acctttcatt ttgaggcgac aaaaaacaac acctatctta atgaaattat
     6301 tgataaaaaa gctggcacaa tgtttgcaga ataagttaaa gtaagttaaa gatcttaaag
     6361 tagatctacc gatctacatt taagatctta aaaacaagaa gtttaataag caaaaaaaca
     6421 caaaaaatct gtggataact ctgctcaaaa caaaaaattc acagcgcctg tggataactt
     6481 tgcgtaaacc cggaggacag atcactctga accaagacaa ccacacataa aaacaatatg
     6541 ctcatttttt aaccactgtg cgtaagtgcc taatgtaatc cctgtgctat tccgcttcct
     6601 cgctcactaa ctcgctacac tcggtcgttc ggctgcggcg agcggtgtct cctttctcaa
     6661 aaccagaaga gttcagctca tacgatccgt tgattgatcc tttttggatc tttcactgag
     6721 aagccctgaa tcgctttctg ccgcttcgtt cgatacaacc atggtgaaac catgcgaaat
     6781 cgaaaaaatc gctcagaatg cgatacagcg cgttttagag ggtatttaca aaacagtcgt
     6841 tgccttgagg aaattttgat caagattgaa ctcctatccg gatcatggca acagatcaca
     6901 gcaacaaggc cgctaaaaag cgttgagcgg cgttaaaaac cgttaaaccg tagctgacta
     6961 caacgaaacg ggaaaaaacg ctcagaacgc gttacagagc gttttaaggc gatgtcggaa
     7021 agagccatat ctcacctgtt ctgatgtttt tctcaacata agcactttta agagtgcttt
     7081 taaagatgct attatgccaa tgagacaagc ggagggctta tgagctatca aattctgaca
     7141 actacagcgg ccagtattac tgacctgaaa aaaaatccta tgggaaccgt agctgaaggt
     7201 gaaggggacg ctgttgcgat cctgaaccga aacgaaccgg cgttctattg cgttccacca
     7261 aaactttacg cctactatcg ggaactcgct gaagatgctg agttaaacgc tgttgctgat
     7321 gagcgcatga aaaacccgga aattgtgaag gttaacctgg atgacctatg aactggcttt
     7381 tgaccgtaga gcactgaagg aatggcagaa actcggccac accatccgtg aacaattcaa
     7441 aaagaaactg gcagaacggc tggaaaatcc acgcgtaccc gcagcccggt tacatggtca
     7501 tgctgatcgc tataaaatca aacttcgtgc atctggctac agacttgtat accaagtcat
     7561 tgatgagaaa gtcgttttac tcgttattgc cgttggaaga agggaaagca gcgaagtcta
     7621 tcagatcgca gatttgcgct aagattaatc atcaaatgat tggtgatgct gccaacttac
     7681 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
     7741 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
     7801 cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta caccgcttct
     7861 caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg atgccgggca
     7921 acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc
     7981 cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg
     8041 acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg
     8101 acaggctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg
     8161 ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc
     8221 cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc
     8281 gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc
     8341 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
     8401 aacactatca ataagttgga gtcattaccc ctttttttga cttctttcat gtgatggcct
     8461 ctgacagaaa taacgtcata gtctctgtta tgtgcaacga ggtgctttgt ccatggttga
     8521 tagcatcaaa atagtttaat caggcaataa tatacttgct tacgaactat gtaatttatt
     8581 gattcattaa gaaacttaaa aagttaaaag atttccggga gtcaacattg aggattaagc
     8641 acgttttcct taatgctact tttagcatct tatctaaaat taaccgctgg tgcgaagtaa
     8701 tacggagata ttatcatgga aaaaataggt aatgacattc cgcatcttat agatcaagct
     8761 atagatttta tggcatcaac ccaggcgttc cgagagtatt tgaaaaaact ccccccccgt
     8821 aacacaattc cgtccgatat tcctgaggaa aacgtgccgt tatatctaca acgtctggaa
     8881 tattatcgcc agctttacag gccaaagtag ataaaatgtg ccctcagagg aaattgttta
     8941 cttttgaaag gtatttttaa gctgattatt tgaaatcaga actgtcagag agaggaccat
     9001 tgttgatcac acagcctgtt gatagagtaa aagaccacct cctgaggagg tggcttttca
     9061 gaatgccccc tataaggggg gcaaatgtca tattccgttt tctactctag caactccatc
     9121 tgctgttcat gtgtttgttc catcttttcc tgatgcctca catatcgtct gataatttct
     9181 tcgttcacgc ctaccgtatc gacaaaataa ccccgcgacc aaaaatggtt tccccataac
     9241 ttcttcctga tatgcgggaa tcgattgtag agcctaattg cactgcgacc tttcaggtgc
     9301 cccatcagag tagatattga gatttttggc ggcactatta cgacaagatg cacggtgatg
     9361 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     9421 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcacc
     9481 gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact gcagttcact
     9541 tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt
     9601 ggatgccggg caacagcccg cattatgggc gttggcctca acacgatttt acgtcactta
     9661 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
     9721 gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt
     9781 tttacgcgta tgacaggctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
     9841 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
     9901 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
     9961 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
    10021 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
    10081 atctgaacat aaaacactat caataagttg gagtcattac c
//
