LOCUS       NZ_CP115152             9730 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Enterobacter hormaechei subsp. xiangfangensis strain MDCL 3 plasmid
            ColRNAI, complete sequence.
ACCESSION   NZ_CP115152
VERSION     NZ_CP115152.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21522362
            Assembly: GCF_028752775.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei subsp. xiangfangensis (Enterobacter
            xiangfangensis)
  ORGANISM  Enterobacter hormaechei subsp. xiangfangensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Halder,G., Chaudhury,B.N., Mandal,S., Denny,P., Sarkar,D.,
            Chakraborty,M., Khan,U.R., Sarkar,S., Biswas,B., Chakraborty,A.,
            Maiti,S. and Dutta,S.
  TITLE     Whole genome sequence-based molecular characterization of blood
            isolates of carbapenem-resistant Enterobacter cloacae complex from
            ICU patients in Kolkata, India, during 2017-2022: emergence of
            phylogenetically heterogeneous Enterobacter hormaechei subsp.
            xiangfangensis
  JOURNAL   Microbiol Spectr 12 (4), e0352923 (2024)
   PUBMED   38385742
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Halder,G., Chakraborty,M. and Dutta,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-DEC-2022) Bacteriology, ICMR-National Institute of
            Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM
            Beleghata, Kolkata, West Bengal 700010, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Assembly Name                     :: Unicycler
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 250.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028752775.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 17:44:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,868
            CDSs (total)                      :: 4,751
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 112
            CDSs (without protein)            :: 112
            Pseudo Genes (ambiguous residues) :: 0 of 112
            Pseudo Genes (frameshifted)       :: 51 of 112
            Pseudo Genes (incomplete)         :: 66 of 112
            Pseudo Genes (internal stop)      :: 20 of 112
            Pseudo Genes (multiple problems)  :: 25 of 112
            Pseudo Genes (short protein)      :: 1 of 112
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115152.1.
            Bacteria and source DNA available from Medical College, Kolkata.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Enterobacter hormaechei subsp. xiangfangensis"
                     /mol_type="genomic DNA"
                     /strain="MDCL 3"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="xiangfangensis"
                     /db_xref="taxon:1296536"
                     /plasmid="ColRNAI"
                     /geo_loc_name="India: Kolkata"
                     /collection_date="2019-03-30"
                     /collected_by="Gourab Halder, ICMR-NICED"
     gene            join(9640..9730,1..2099)
                     /locus_tag="MUY00_RS24295"
                     /old_locus_tag="MUY00_24290"
     CDS             join(9640..9730,1..2099)
                     /locus_tag="MUY00_RS24295"
                     /old_locus_tag="MUY00_24290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            3685..5646
                     /locus_tag="MUY00_RS24300"
                     /old_locus_tag="MUY00_24295"
     CDS             3685..5646
                     /locus_tag="MUY00_RS24300"
                     /old_locus_tag="MUY00_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5646..6377
                     /gene="mobC"
                     /locus_tag="MUY00_RS24305"
                     /old_locus_tag="MUY00_24300"
     CDS             5646..6377
                     /gene="mobC"
                     /locus_tag="MUY00_RS24305"
                     /old_locus_tag="MUY00_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6384..6914
                     /locus_tag="MUY00_RS24310"
                     /old_locus_tag="MUY00_24305"
     CDS             6384..6914
                     /locus_tag="MUY00_RS24310"
                     /old_locus_tag="MUY00_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6942..7121)
                     /locus_tag="MUY00_RS24315"
                     /old_locus_tag="MUY00_24310"
     CDS             complement(6942..7121)
                     /locus_tag="MUY00_RS24315"
                     /old_locus_tag="MUY00_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7190..7567
                     /locus_tag="MUY00_RS24320"
                     /old_locus_tag="MUY00_24315"
     CDS             7190..7567
                     /locus_tag="MUY00_RS24320"
                     /old_locus_tag="MUY00_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(7629..7928)
                     /locus_tag="MUY00_RS24325"
                     /old_locus_tag="MUY00_24320"
     CDS             complement(7629..7928)
                     /locus_tag="MUY00_RS24325"
                     /old_locus_tag="MUY00_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7918..8181)
                     /locus_tag="MUY00_RS24330"
                     /old_locus_tag="MUY00_24325"
     CDS             complement(7918..8181)
                     /locus_tag="MUY00_RS24330"
                     /old_locus_tag="MUY00_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8558..9643
                     /locus_tag="MUY00_RS24335"
                     /old_locus_tag="MUY00_24330"
     CDS             8558..9643
                     /locus_tag="MUY00_RS24335"
                     /old_locus_tag="MUY00_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(2305..2885)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5647..6374
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 atatttcacc agaagaaaca ttaattgttc atgggaccgc tgtttctgaa agcacaacag
       61 aaggaacaga ttcatacaca acccagtcag ccaatacagc atcaaagatg aatctgtcat
      121 tccgggatac acctcagtca actacagtca ttacgcgacg aagaatggat gatgtagcag
      181 caaaaaatat tattgatgtg gttaaaaccg ctcctggatt atatgtcagt acagcagaag
      241 gttccggaag accgaatttt atgtccagag gatttaatgc taatgtcatg tatgaaggat
      301 ttagcctgcc gtggaattca tacattccaa gttcacaaac tagttcagct atttttgacc
      361 gggtggagat agtccgcggg gcaacaggtc ttgttcaggg tgccggagac ccttcagccg
      421 cgataaatat gatacacaag cgtccgaccc ggcaatatca ggccagcact aacatcggtg
      481 ccggacggtg gaatgactac cggggtgggg ttgatgtatc aggaccactg aatgacagcg
      541 cgaccttacg cggaagggca gttgtggatg gcgaaaacag tgagaccttc agggatagtg
      601 agaaacgcag gaataacgtc atttacggaa ttatggaggc agatctgact gataataccc
      661 tacttacgat tgggggatat cagcagtatg aatataacaa tcattggtgg tatgacttac
      721 ctatttctgg taccggacac caccttaatc ttcctcgctc gacgtttaca ggcaatgact
      781 gggagtttga aagaagtaaa agtaaaactg catttgcaac tcttgagcag aagttcagta
      841 atagctggaa gctgaaattt tcaacgctgc aaaactggag aaatgctgac ctgttaggga
      901 cggcaacata ccggcataaa gatacccgac aaagtatgta tcagcaagta tggggatcac
      961 tgagggaata taattccaaa agctatgacc tgtcgcttaa tggggactat gctttatttg
     1021 gtcgtgttca tcaattcgtg gctggaacaa ctcagcaaaa atacaccgat aaagatacag
     1081 gttatacata ttccccaata tatattgaga atgttaatat atttgatcat ggttcacatt
     1141 cttcgaaaaa acccatgaag atgtcaggtg atgcatccta cgacgtcact gaacaaaaaa
     1201 gcgcctacgc agcaactcat tttctactta gtgataactg gaaaatattg ctgggtggcc
     1261 gtcttgactg gtatgaatat acaagtaaag gtacatccat taatacaggt tataaagtta
     1321 ccagaaatat caccaaatat gcaggttcaa catacaatct tgacgatcat cataccattt
     1381 atgggagcta taccgatata ttcaatccac agtcaagcac cgacctcaat ggtaaagtga
     1441 tcaagcctat tgttggtaag aattatgaga caggtattaa gggtgagtat tttaacggct
     1501 tgcttaatgc ctctctggcg gtatttcagg tagaccagga aaatcgagcc aggctgcttc
     1561 ccgatcagac agggtgtgtt actaatggca atcctcaatg ctatgaggct agcggtaaag
     1621 tacgcacaaa agggatagat ttcgaacttc agggcatggt tacgccactc tgggaaattg
     1681 gggtgggata tacatatgca gatacgcgat atataaaaga tggagatcgt agtaatgaag
     1741 gcaagcgatt caatactgcc gtgtcgccac aaaatttatt taagatttcg agcatgtaca
     1801 cctttgacgg aaaattaaca aactggcgag caggagtaaa cacgtactgg caaagtaaaa
     1861 tatatgctaa cataacagac acaagggatg gcaatccggt tattaataaa caaaaggctt
     1921 attcaacaac tgacattatt attggctata ctccaatgcc acaactggat ttacagctca
     1981 atataactaa tgtctttgat aaggtttatt atacatccat aggtgaatcg attggatatg
     2041 gcacggaaac atatggagaa ccaagaaatg ttaaaattag tgcaacctat agattctaac
     2101 catgcggatt gggtcttctc ctcggatttg cccctataaa tccgtctttt gtggttccgg
     2161 cgtacaaccc cctgtttgcc acttcagttg atggttaatc aaatcatgta gaggatcttc
     2221 ttgagatcct ccatggttcg tcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
     2281 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
     2341 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
     2401 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
     2461 tgccagtggc gtttttgcgt gtctttccgg gttggactca agtgaacagt taccggataa
     2521 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac
     2581 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgtaagg
     2641 gagaaaggcg gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg
     2701 agcgacccgc cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca
     2761 gattcatggt tgagcctcac ggctcccaca gatgcaccga aaaagcgtct gtttatgtga
     2821 attctggcag gagggcgaag cctatggaaa aacgccaccg gcgcggccct gccgttttgc
     2881 ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgcg agagttagca
     2941 gcgcccgccg cagtctcacg gccggagcgt agcgactgag tgagggagga ggcgcaatat
     3001 cccccctccc gcagacggcc accggacgcg cggatgtgac atattgcacc gtgttaccgc
     3061 ctttcccttc cctgccgata aatcattcag ataggtgaag tctgtcggtt ttctgctgaa
     3121 tattttacgt taacctatct ctgttgcttt tttctccttg cggagaaatg caggtcatcc
     3181 ccgctggggc gtggctgact tcaggaggaa atcccctgct tacgctggct ggacgccacc
     3241 gacgcacggt ctttccccct ctttctggct cagctcggct ggacgccggc cacgccagaa
     3301 attcacccgg cccccgtcag gctcacgcct gaccacccac aaaaacccac ggtgctgaac
     3361 gcacctgacg aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg
     3421 cgaactctga cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc
     3481 tcacacgctg gggcgtgctc gttgttcctc cggggctgcg ccttttccgc ttggggcgga
     3541 aagaggctgg cctccgtggt gaaagctgaa tgcaatcacc acggacgtga ccgccgccgc
     3601 catgcctcgg ggcatgtccg cggcttgtgc agcaggggct gcacaccacg ccggtaaacc
     3661 cgtcgtctga aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat
     3721 atcccggagc tggcccggca gatttacccg tgggtcagct ggctgtacag ggagtattac
     3781 ggtctggccg gggctttcct ggttatcggc ctgtttttcg gtctggctga actctgggcc
     3841 ggacggatgc gttttgtggg gctgagtcat aaggagcgac gggagtatgc cgatcgctat
     3901 aagcgcgata ataaccggac tgcgccattt tcggtgctgg cttcgctgga ttcgcccggg
     3961 cggatggtca gggcacggcg gcatgtctgg aactacgcgt actggctgac cggcgctgcc
     4021 ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc
     4081 gtcctgcgtc cggacctcta cgcgaacgca gattattacc tgctgcagcc ggctaacctg
     4141 ttcgggattg tctggtgtgc cggagggctt gtcgccggca tccttctggc gcggctgctg
     4201 gcatttctgc tgcttgacgg tcattttgcg gccgctgacg aggcggtgaa tgcgaaactg
     4261 aaccaggaga gccggcgcag cagccagcgg accggagaaa tgaccgacgt ccgccacctg
     4321 catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacaga gcaggcccgg
     4381 aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtcccgcgt
     4441 gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgtg
     4501 atgggcacca acgcgctcat tcgctgcgta cgcgatttcg gggacgcggt ggtgtacttc
     4561 gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc
     4621 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgtttcgcga tctcgaccag
     4681 tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg
     4741 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag
     4801 ggggcaaaca ttcagcaggt gctggcggcg gcatacgcgc tgccggaggc cctgaaaaag
     4861 gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg
     4921 gacagcggca tcgacgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc
     4981 tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa
     5041 atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag
     5101 attaaatacc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagtcgggac
     5161 tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg ggcagtgcgg ccaggatctg
     5221 cccgctgagt ttgtgaaaac cacggtgctg gataacacgc cgatccgctg gttctaccgg
     5281 gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggaaat ccgggtggac
     5341 gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcatatcag cggggacagc
     5401 ttcattcaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcagca cctgcctgac
     5461 gggtttgcgg tgatgaccgg gctgggcgtg gcccggatgg cgttcagcag cccgctgcgg
     5521 gttgaacgcc gggaaatccc gctgcagcgc ttcccggtgc tggcgaaaac ggacccgctg
     5581 gcggagtacc agccggaggc cgggcggcag cggccgggtg atgatgattt tgcggggctg
     5641 tactgatggc actggagcga tacaacgtca gccacgcgaa gcggcaggcg cggaacgcgg
     5701 agaaaacccg gctgaccctg cgctggctgc gggaagagct gtgttccacg gcggagctgg
     5761 ttgcgcgacg gctggggatt gcggcggtgc agccggttta ccgcttcctg gacagcctgg
     5821 tggcgaaagg gctgctggtc cgggcgaaat acccggttga cggccgtcag gtcagcgtgt
     5881 ggggactgac gccgcacggg gtggcgttct cgttcgacga ggacgaaccg ctgacggacg
     5941 ttattccgtt tcagccgtca agggtgtcgg cggcgcagct gccgcaccgg ctggccgtgc
     6001 agtccctgcg tctcgccatg gaggcccggg gggcctccgg ctggcggtac ctgcaccgta
     6061 tggccctgaa ggggatgaag gtgccggacg cgctggcgga gctggacggc agaacggtgg
     6121 cgtttgaggt ggagcgcacc gtgaagagcc gcaggcgcta tcaggaggtg gtgtccgggt
     6181 atctgttcaa tcgcaaagcg aacggcatcg atgaaatctg gtacatctgc ccggaccggg
     6241 cgacgcaggt cagggtgcag cgggcgatcc tgtcggtgga tgaaatcgtg aatccacaga
     6301 ccggcgaggc ccgcaaaacg gcggagctgg accgggagcg gctgtttgcg tgctttaaat
     6361 ttatgacaac ggagtgaggg attatggcgc tgtacatggt atttattgaa gacgcggagg
     6421 accgggacgg cgagcgctgc atcatgccga ccagcgagca tcttgaagat atggtggtgc
     6481 tcgcctggca gaccggggcg tatgttttct gttttgtcga ccgggcccag catctggcga
     6541 tgatgaaatc catcgggatg tatgacgagc ggctcaacag cgacgaaaaa gccacgtact
     6601 ttttcaccgg taaaaatccg caatgggaac gggacaaacc cgcaggaatg aagctgtgtg
     6661 attacctcta tcgcaaggtg gagcggttcg ccatcgagaa cggtgtgcag gtggttgatg
     6721 ccgggaagtg ctcaccggag cagattagcg cgatgattga ggtggtcagc gagcggcacg
     6781 gggaaagcgc ggtgaacgag aagctgaaag cggagctgat gcgggaatat aacagccgct
     6841 attttaacgt gccggtgcct gaactgccgg aggacaaacc cgcccgccgg tggtggccgt
     6901 tcggcaaaaa ataaacggca gcaccggcca tgccggtgct gttattcctt cccgagctta
     6961 tcccgcatca cctgccagag tgtttccgca tcctcgtgca gccgctcgca catgtcggcc
     7021 ggggtatcca tatccagctc gttcagtttc tcgagtaaca gcagcgactg gtccttaata
     7081 aatttagcca tattgagggc agttttttcc tgcttagtca tgttttctgt ccttccgggt
     7141 gtggtgtgct tcctgtcggg tatactgtct caaaaccgga ggtgagacaa tggcgtgggt
     7201 gacggtgaga caggcgacgg aactgacggg gaaagcccgg agttccctgt atagggatat
     7261 ggcgaaaggc cgcgtatcat accgtactga agctgatggt ggtcgggtgg tggataccag
     7321 cgaacttatc cgggtgtacg gtgaattaag acagattgag actcacgagc gggacgggtt
     7381 gagactgtcg gatgagactg aaaaaatggc ctccgacgcg ttgatcgcgg agataaaagc
     7441 actcagggag gaggtggccg gactgcggca ggaaatgcag gccatgagac tgctggaaca
     7501 caaaccagag acagcagaga caccggccgg aacatcgcgc cgatggtggc agtggggaaa
     7561 acgctgacgc gctgtgcttg tccaaccggc gacggccacg caatgaattg cgcccccatc
     7621 accggttatc agcgaacggt atggggattc tggcgctggc caaccacggc cagaacccgg
     7681 actttcctgg tctggtcatc aacatcatag aggacatgct gtcccatcag actgatccgg
     7741 cgaacgtcct cgccatagcg tgagtccact tcatataggc gcgggaagtc ggccagtttg
     7801 tccgctgcag cgagaactcg atcggtaacg ttctgcgcat cccacaggga tgaattttcc
     7861 gcaatgtagt caaacaatgc tgtcacatcc tcgatggctc cctgcttcca ttccacctca
     7921 tacggcatcg cgtagttcct gatagcggcc atgcagtctc tggcggagtt gttccgtggt
     7981 catgcccggg cgcgtgtcag cgcgggcggc ggtcacttta gcgcgaacct gatcttcata
     8041 ttctgcctgg gccagttctt cgttagccag gcgctgcaac agtgcagctt tgtccgggcc
     8101 accttcaggc agtatgcggg gcagtctctc ggccagttcc cgcaggaaat tggcgttttc
     8161 atagtgtgta ctgttgctca ttgtggagcc tcccgggctg atacgtcgat atgaaggagt
     8221 ttagcagggg gcggtctgaa agaatatgct cgtgtcagtc aggctgatga agacgaaagc
     8281 tggtaaaaaa gggcttacga cacccatttt acgtcaaact gcacgacatg cagttgcgtg
     8341 catagccatg cagcgggtta aaatttatca ggcgcgtttc cggcggtttt ccgggtggtt
     8401 tgttgccggt tttatcccgt agccgccgga aatgccctca gaccgtctga gcggtgcgcg
     8461 taatgcgacg ttatggtaaa tcatgtgatt aatggtagtg ataatcatta tcacttttgc
     8521 tatattcttg tttcttttat tgtaaggaaa tgtaagtatg gcaatcacga ttaaatccgc
     8581 cgttaatgtc cgtagcagag tattaatata ctctttacta ctgtttactg gttcagcgta
     8641 tgcggctgat atagctgaat caacattcct gactcgtgat atcggcaatg gtctgtatga
     8701 aatgtctttt gatggcaaaa ataatagtct ctgggcagca tcatcgtctt cattcgacag
     8761 agataagggc cacggaattg tttatcagtt agaaccatcc actctgaaaa caaaatctgc
     8821 cgttaaaatc gcacgtcttg ggtttgccac agcactggat gaggaacatg agcgacttta
     8881 tatcggcaat actctggacg gctcagtgac ggtcattgat actctaaccg gaaaagaact
     8941 ttcagtaatt ccgttaactg agggacagga tgaggctcat ttcagaccga ccagagaaat
     9001 ggtcattgac aaaaaaaatc agcgtcttta tgtctccagc gccgcagata aaggggctct
     9061 gtgggttatt gacatggctg tcgggaagaa aattaaaacc attgatggac tggggcacgc
     9121 tgttactgga attgcgttaa ataaaaatgg ggataatatt tatctggtta acggggatgg
     9181 tgagattatt aacctggatg ccagtagcta tgaaataaaa aaacggtttg ttgtagagcc
     9241 tgagaaaaaa cactttttcc ttaatgtaag tctggatgaa aaaaacggac gtgcatttat
     9301 taccgacccg gatattcccg gggtgctggt tgttgatgta aactcgggaa aaatactaca
     9361 cagaattgat gttataaatt cactggcgat tttatttaat cctttacgga acgaaatcta
     9421 tatcacgcac cgtaatgcaa aacaggtcag cattgtggac agtaattctt attacgtaaa
     9481 aacaacgata aaaacccgcc tgatgccaaa cagtctgtct ttatcacatg acggtgaaaa
     9541 tctgtacgtt agtgtaaaac aagaaaaaaa agagatgagt actatgaagg attacatact
     9601 caaaatcgaa ttatctaaaa ttgctatgga gccagaagaa tgaataaata tttagggctg
     9661 agcatagtat atatgatgta tacatcatct gttatggctg atgattctgg cgcagttagt
     9721 aaagataaaa
//
