LOCUS       NZ_CP115020             4947 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Serratia nevei strain CUVET18-1784 plasmid pCUVET18-1784.5, complete
            sequence.
ACCESSION   NZ_CP115020
VERSION     NZ_CP115020.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262098
            Assembly: GCF_030863845.1
KEYWORDS    RefSeq.
SOURCE      Serratia nevei
  ORGANISM  Serratia nevei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:55:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,680
            CDSs (total)                      :: 5,556
            Genes (coding)                    :: 5,430
            CDSs (with protein)               :: 5,430
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 34 of 127
            Pseudo Genes (incomplete)         :: 92 of 127
            Pseudo Genes (internal stop)      :: 18 of 127
            Pseudo Genes (multiple problems)  :: 15 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Serratia nevei"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-1784"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:2703794"
                     /plasmid="pCUVET18-1784.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-11-11"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(4511..4947,1..61)
                     /locus_tag="O8I24_RS28375"
                     /old_locus_tag="O8I24_28360"
     CDS             join(4511..4947,1..61)
                     /locus_tag="O8I24_RS28375"
                     /old_locus_tag="O8I24_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142674820.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSELEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAAILWWQGNQML
                     DNYASIREQRDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGTKIGIRLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            68..283
                     /locus_tag="O8I24_RS28380"
                     /old_locus_tag="O8I24_28365"
     CDS             68..283
                     /locus_tag="O8I24_RS28380"
                     /old_locus_tag="O8I24_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            554..1468
                     /gene="lpxO"
                     /locus_tag="O8I24_RS28385"
                     /old_locus_tag="O8I24_28370"
     CDS             554..1468
                     /gene="lpxO"
                     /locus_tag="O8I24_RS28385"
                     /old_locus_tag="O8I24_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(2187..2354)
                     /locus_tag="O8I24_RS28390"
                     /old_locus_tag="O8I24_28375"
     CDS             complement(2187..2354)
                     /locus_tag="O8I24_RS28390"
                     /old_locus_tag="O8I24_28375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222169324.1"
                     /translation="MTRDKNDRESPEEPADRGTRDDKSSQNDKGGDWIGELERELQEIT
                     KKWPGLRSLS"
                     /name="hypothetical protein"
     gene            3510..3833
                     /locus_tag="O8I24_RS28395"
                     /old_locus_tag="O8I24_28380"
     CDS             3510..3833
                     /locus_tag="O8I24_RS28395"
                     /old_locus_tag="O8I24_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_192869793.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4042..>4476
                     /locus_tag="O8I24_RS28400"
                     /old_locus_tag="O8I24_28385"
                     /pseudo=""
     CDS             4042..>4476
                     /locus_tag="O8I24_RS28400"
                     /old_locus_tag="O8I24_28385"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3224..3407
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.702"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(2766..3241)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.834"
                     /coverage="92.0"
                     /evalue="5.88e-172"
     Typing          2..381
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.395"
                     /coverage="100.0"
                     /evalue="3.75e-53"
     Typing          3824..4947
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.133"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggatacgaac tggacgttcg acaacggaac gaagatcggc atcaggctgg tacgggagta
       61 gatcagaatg acagagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga
      121 ctactcgaaa aggctggacg agtgggagag cgccttcgcg gaatggcgga cgatgtctgg
      181 tcttatgcaa cgggagaacg cggcgctgag cgagcgcgtc acgggcttga gcaggcaggt
      241 gcagagtttg agcgatcagc tgcgccggtt gtcgagcggc tgaatgccgt cgagctgcag
      301 caggtgcggg aggcgcggca tcagaaagcg ctggagctcg aacgatcaca gcgacagcgg
      361 gtatatcacg gtccgtccct ttgattcatt tcagcataat tcctgctgaa atcctctctg
      421 cctgtgtagt agatatgttc tggtgaagct tcgattatac tattgataaa tagcatttag
      481 attataattt aagtgcattc attatgttat ctttaagaga tttttattat tggcgtgaac
      541 aaggaatata cacatgctgt cacttattat tatttttgtt tttcttatca gtgtattgta
      601 tgcgcattct cgcggtaaaa taaaacaaaa gttttccagg cagttatttg atcattcaac
      661 atttatggct ccgataaata tgtttatgac aggcttttcg aagctgccgt caaagcaggc
      721 atggtttccc ccagaaacat ttcctgaact tcaaccatta attgataact ggcaggttat
      781 aagagacgaa gctgttaatt tgcaagggca cattaaatca tctgaaaaaa ataatgatgc
      841 aggatttaat acatttttta agcgcggttg gaagcgcttt tacctcaagt ggtatggaga
      901 tgctcatcca tcagcggagc ggctttgccc agtaacaacc caactggtta gctctattcc
      961 ctgtattaaa gcagcgatgt ttgctgaatt gccttcagga agccatttag gaaagcatcg
     1021 tgacccttat gccggttcag ttcgttatca tttaggatta tcaacaccaa atgatgaacg
     1081 gtgttttatt gaagttgatg gtgtacgccg cagttggcat gatggtgaag tgataatttt
     1141 tgacgaaact tttgttcact gggctcagaa tgataccgag cagacacgta ttattttgtt
     1201 ctgtgatata gaaaggccga tgaagtggcg gtggtcacaa aaaataaata ggtggatagg
     1261 tggggtgcta atgtcagcgg cagcatcacc caacgataat aatgacagga ctggtgctat
     1321 aaatcattta tttaaatatg ttaatgctat tcgtgatgca ggtcagaagc taaaaaaaag
     1381 gaatcgaaaa ctatattatt tgattaaaaa tggagttatt tctttgattt ttgtttggct
     1441 tgctgtctca gtgtttagat ggctgtgatt tgtttttttc tattgagtca tacataaatt
     1501 tgggtcttct atgctcaatg tatggttata gataaaaatc gcctgctaaa aaattctatt
     1561 gaaatctttt tagtaagaaa ggcccgaatc tgacgctcag cgggcctttc gtgaagaagt
     1621 gcggcagtag acagatttca tctgatcacg ttcatgtgct gctgtgcaat taagcgtttc
     1681 tttcggagct ttcacggtat ttccttcacg aaaccggccg ccattcactc ttgtgcatag
     1741 ccatgcagcc gcgcagaaat tatcaggggc gtttttcagg gtttgggccg gggaaaggtg
     1801 ccggaaacgg cccggacgcg gcagaaacgc gctgaggagc tctgcggggg tgcggccaag
     1861 agatgttatg ttaaaagcgt gacgcccggc aggtacgaag aaaaaagatg gcagctgcgc
     1921 ttatgtgcct gcagagaagg tcgataacgg tagtgtgcct caccagaggt gcataaaccg
     1981 atactgctgc tgtatgtgct gaaggctccc gctgcgggga gcctttcttt tgccctgtgc
     2041 ggtctgcggt ggacttgcgg aagcgccttt ttattacaca ctttaagtgt gccgttccat
     2101 gctggtttcg gcacgttgct gtgtattttg aacgcgctac gtcgttcagc ccgctactaa
     2161 atatggcggg tttttacggt catgattcag ctcaggctcc gtaagcctgg ccacttcttc
     2221 gtgatctcct gcagttcccg ctccagctcg cctatccagt ccccgccttt gtcgttttgt
     2281 gagcttttat cgtcgcgggt gcctctgtct gcaggctctt ccggcgattc ccggtcgttc
     2341 ttgtcacgtg tcatctgtgt ttcccccttc agccccggga tattttcggc gagaaggggt
     2401 tacactcatg cgcgaagcgg gtgaagttat gaccagcatt cagaggaatg ctgcaggggt
     2461 tgagactgcg gttaaggatg tcgccatttc agttaaagcc ggataggcca tggtgcatat
     2521 ccccatatct gtcgaacatc acagatgatc ttcttgagat cctttccgtt gcgtcgtaat
     2581 cttttgctct gaaaacgaaa aaaccaccct gcaaggtggt ttttcgaagg ttatttaatc
     2641 ctggcagatt atctaaccgc ggtaacagtc ttgtgcgaga catgccacca aatcagtcct
     2701 tccagtgtag cctcagttag gccactactt caagaactct cgatacatct ctcgcacatc
     2761 ctgtttacca atggctgccg ccactggcga tttttcgtgt ctttccgggt tggactcaag
     2821 ttgatagtta ccggaatggg cgtggtagtc ggactgaacg gggggttcgt gcatacagtc
     2881 cagcttggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga gataaacgcg
     2941 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     3001 agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc cgccactgat
     3061 ttgagtgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcaacg
     3121 ccgcatcctt tttccggctt ttcttgagcg ccatctcctt atctcgtcat tcgtgagcag
     3181 gtaacgcccg ccgcagtctc acggcaggag cgtagcgacc gagtgagcga ggaggcgtca
     3241 tttcacctac accggcattt cgcacttacg ccccacagcc gcatttttta cttaccacag
     3301 gaagcacttt cctgaattcc cacttcgacc taccatatta agccagtata cactccgcta
     3361 gcgctacgtg actggttcag gcctgcgccc cgaaacccgc taaaaccact gacgcgcctg
     3421 cggcttgccc aacaccgcgc cgaccggaaa aagatttccc gccgtcccgg cgttatcagg
     3481 aggccggatt ggcagacaaa cgcagcaaaa tgctcacgat gtgggtgacc gaagatgagc
     3541 accgccgtct gctggaacgc tgcgacggta aacagctcgc ggcctggatg cggcagacgt
     3601 gcctggacga gaagcctgcc cgcgccggca aactcccctc gatctcgccg gcgctgcttc
     3661 gtcagcttgc cggcatgggg aacaacctta accagattgc ccggcaggtt aacggcggtg
     3721 gcggcagcgg gcacgaccgc gtgcaggtag tcgccgcact gatggccatc gatgccggac
     3781 tcgagcggct gcggcatgcc gtgctggaaa agggggcaga cgatgatcgt taagtttcac
     3841 cccagggggc gcggcggtgg cgctggtccg gtggattatc tgctgggcaa agaccgccag
     3901 cgcgaaggtg ccagcgttct gcaggggaag ccggaggaag tccgggagct tatcgacgcc
     3961 tcgccctacg ccaaaaaata cacctccggc gtcctgtctt ttgccgaaca ggacttaccg
     4021 cccggccagc gtgaaaagct gatggcgagc ttcgagcggg ttctgatgcc cggactcgat
     4081 aaagatcagt acagcgtgct gtgggttgag caccaggaca aagggcggct ggagctgaac
     4141 ttcctgatcc cgaacacgga gctgctgacc ggcaggcgtc tccagccgta ttacgaccgc
     4201 gccgaccgtc cgcgcattga tgcctggcag accatcgtga acggcaggct ggggctgcat
     4261 gacccgaacg cgccggaaaa ccggcgggcg ctggtcacgc cgtccgcgct gccggaaacg
     4321 aagcaggaag ccgcccaggc gattacgcgg ggtttactgg tcttagcctc gtccggggag
     4381 ctgaaaaacc gtcaggacgt cactgaggcg ctggaaagcg cgggttttaa ggtggtgcgc
     4441 accacaaaaa gcagcatcag cattgccgac ccggacgggg ggcgaaatat ccgactgaag
     4501 ggagccattt atgaacagtc ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc
     4561 gcagcagcag actaccggcg agatgctgaa agccgcattc agcgagctcg agaagtctgt
     4621 cagagcggaa ctgagcgaaa gcgagaagag aatcagcgcc gccatcctcg accacgaccg
     4681 gaagctgtcc tcagccatga gccagcgcac gaaaggcatg gtgcgtatgg tcagccagac
     4741 gtggctgacc atcgtcctgg tctccgcgct actgatagcc tcgagcgcgg ccattctgtg
     4801 gtggcagggg aaccagatgc tcgacaacta cgcgagcatc cgggaacaga gggacgcgct
     4861 ggagaagctg aacgcccgga cgtggggcgt gaggtaccag gaggacaacc aggggcgttt
     4921 tctggtgctg ccggagggcg tgaaagc
//
