LOCUS       NZ_CP115014             4947 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Serratia nevei strain CUVET18-1371 plasmid pCUVET18-1371.5, complete
            sequence.
ACCESSION   NZ_CP115014
VERSION     NZ_CP115014.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262097
            Assembly: GCF_030863825.1
KEYWORDS    RefSeq.
SOURCE      Serratia nevei
  ORGANISM  Serratia nevei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 06:27:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,508
            Genes (coding)                    :: 5,373
            CDSs (with protein)               :: 5,373
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 135
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 38 of 136
            Pseudo Genes (incomplete)         :: 97 of 136
            Pseudo Genes (internal stop)      :: 18 of 136
            Pseudo Genes (multiple problems)  :: 15 of 136
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Serratia nevei"
                     /mol_type="genomic DNA"
                     /strain="CUVET18-1371"
                     /isolation_source="wound"
                     /host="dog"
                     /db_xref="taxon:2703794"
                     /plasmid="pCUVET18-1371.5"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2018-09-07"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(270..485)
                     /locus_tag="O8I25_RS28135"
                     /old_locus_tag="O8I25_28120"
     CDS             complement(270..485)
                     /locus_tag="O8I25_RS28135"
                     /old_locus_tag="O8I25_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(492..989)
                     /locus_tag="O8I25_RS28140"
                     /old_locus_tag="O8I25_28125"
     CDS             complement(492..989)
                     /locus_tag="O8I25_RS28140"
                     /old_locus_tag="O8I25_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142674820.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSELEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAAILWWQGNQML
                     DNYASIREQRDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGTKIGIRLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1024..1728)
                     /locus_tag="O8I25_RS28145"
                     /pseudo=""
     CDS             complement(<1024..1728)
                     /locus_tag="O8I25_RS28145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1667..1990)
                     /locus_tag="O8I25_RS28150"
                     /old_locus_tag="O8I25_28135"
     CDS             complement(1667..1990)
                     /locus_tag="O8I25_RS28150"
                     /old_locus_tag="O8I25_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_192869793.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNGGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3146..3313
                     /locus_tag="O8I25_RS28155"
                     /old_locus_tag="O8I25_28140"
     CDS             3146..3313
                     /locus_tag="O8I25_RS28155"
                     /old_locus_tag="O8I25_28140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_222169324.1"
                     /translation="MTRDKNDRESPEEPADRGTRDDKSSQNDKGGDWIGELERELQEIT
                     KKWPGLRSLS"
                     /name="hypothetical protein"
     gene            complement(4032..4946)
                     /gene="lpxO"
                     /locus_tag="O8I25_RS28160"
                     /old_locus_tag="O8I25_28145"
     CDS             complement(4032..4946)
                     /gene="lpxO"
                     /locus_tag="O8I25_RS28160"
                     /old_locus_tag="O8I25_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     Typing          complement(2091..2277)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.387"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          2254..2735
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.549"
                     /coverage="93.0"
                     /evalue="5.88e-172"
     Typing          complement(173..1677)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.095"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtatattcc ttgttcacgc caataataaa aatctcttaa agataacata atgaatgcac
       61 ttaaattata atctaaatgc tatttatcaa tagtataatc gaagcttcac cagaacatat
      121 ctactacaca ggcagagagg atttcagcag gaattatgct gaaatgaatc aaagggacgg
      181 accgtgatat acccgctgtc gctgtgatcg ttcgagctcc agcgctttct gatgccgcgc
      241 ctcccgcacc tgctgcagct cgacggcatt cagccgctcg acaaccggcg cagctgatcg
      301 ctcaaactct gcacctgcct gctcaagccc gtgacgcgct cgctcagcgc cgcgttctcc
      361 cgttgcataa gaccagacat cgtccgccat tccgcgaagg cgctctccca ctcgtccagc
      421 cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt ttccagctct
      481 gtcattctga tctactcccg taccagcctg atgccgatct tcgttccgtt gtcgaacgtc
      541 cagttcgtat ccgctttcac gccctccggc agcaccagaa aacgcccctg gttgtcctcc
      601 tggtacctca cgccccacgt ccgggcgttc agcttctcca gcgcgtccct ctgttcccgg
      661 atgctcgcgt agttgtcgag catctggttc ccctgccacc acagaatggc cgcgctcgag
      721 gctatcagta gcgcggagac caggacgatg gtcagccacg tctggctgac catacgcacc
      781 atgcctttcg tgcgctggct catggctgag gacagcttcc ggtcgtggtc gaggatggcg
      841 gcgctgattc tcttctcgct ttcgctcagt tccgctctga cagacttctc gagctcgctg
      901 aatgcggctt tcagcatctc gccggtagtc tgctgctgcg ctttcgattt ctgctctaag
      961 tccttcgcca gcgttaaaag actgttcata aatggctccc ttcagtcgga tatttcgccc
     1021 cccgtccggg tcggcaatgc tgatgctgct ttttgtggtg cgcaccacct taaaacccgc
     1081 gctttccagc gcctcagtga cgtcctgacg gtttttcagc tccccggacg aggctaagac
     1141 cagtaaaccc cgcgtaatcg cctgggcggc ttcctgcttc gtttccggca gcgcggacgg
     1201 cgtgaccagc gcccgccggt tttccggcgc gttcgggtca tgcagcccca gcctgccgtt
     1261 cacgatggtc tgccaggcat caatgcgcgg acggtcggcg cggtcgtaat acggctggag
     1321 acgcctgccg gtcagcagct ccgtgttcgg gatcaggaag ttcagctcca gccgcccttt
     1381 gtcctggtgc tcaacccaca gcacgctgta ctgatcttta tcgagtccgg gcatcagaac
     1441 ccgctcgaag ctcgccatca gcttttcacg ctggccgggc ggtaagtcct gttcggcaaa
     1501 agacaggacg ccggaggtgt attttttggc gtagggcgag gcgtcgataa gctcccggac
     1561 ttcctccggc ttcccctgca gaacgctggc accttcgcgc tggcggtctt tgcccagcag
     1621 ataatccacc ggaccagcgc caccgccgcg ccccctgggg tgaaacttaa cgatcatcgt
     1681 ctgccccctt ttccagcacg gcatgccgca gccgctcgag tccggcatcg atggccatca
     1741 gtgcggcgac tacctgcacg cggtcgtgcc cgctgccgcc accgccgtta acctgccggg
     1801 caatctggtt aaggttgttc cccatgccgg caagctgacg aagcagcgcc ggcgagatcg
     1861 aggggagttt gccggcgcgg gcaggcttct cgtccaggca cgtctgccgc atccaggccg
     1921 cgagctgttt accgtcgcag cgttccagca gacggcggtg ctcatcttcg gtcacccaca
     1981 tcgtgagcat tttgctgcgt ttgtctgcca atccggcctc ctgataacgc cgggacggcg
     2041 ggaaatcttt ttccggtcgg cgcggtgttg ggcaagccgc aggcgcgtca gtggttttag
     2101 cgggtttcgg ggcgcaggcc tgaaccagtc acgtagcgct agcggagtgt atactggctt
     2161 aatatggtag gtcgaagtgg gaattcagga aagtgcttcc tgtggtaagt aaaaaatgcg
     2221 gctgtggggc gtaagtgcga aatgccggtg taggtgaaat gacgcctcct cgctcactcg
     2281 gtcgctacgc tcctgccgtg agactgcggc gggcgttacc tgctcacgaa tgacgagata
     2341 aggagatggc gctcaagaaa agccggaaaa aggatgcggc gttgccgttt ttccataggc
     2401 tccgcccccc tgacaagcat cacgaaatct gacactcaaa tcagtggcgg cgaaacccga
     2461 caggactata aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc
     2521 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc
     2581 ctgacactca gttccgggaa ggcagttcgc tccaagctgg actgtatgca cgaacccccc
     2641 gttcagtccg actaccacgc ccattccggt aactatcaac ttgagtccaa cccggaaaga
     2701 cacgaaaaat cgccagtggc ggcagccatt ggtaaacagg atgtgcgaga gatgtatcga
     2761 gagttcttga agtagtggcc taactgaggc tacactggaa ggactgattt ggtggcatgt
     2821 ctcgcacaag actgttaccg cggttagata atctgccagg attaaataac cttcgaaaaa
     2881 ccaccttgca gggtggtttt ttcgttttca gagcaaaaga ttacgacgca acggaaagga
     2941 tctcaagaag atcatctgtg atgttcgaca gatatgggga tatgcaccat ggcctatccg
     3001 gctttaactg aaatggcgac atccttaacc gcagtctcaa cccctgcagc attcctctga
     3061 atgctggtca taacttcacc cgcttcgcgc atgagtgtaa ccccttctcg ccgaaaatat
     3121 cccggggctg aagggggaaa cacagatgac acgtgacaag aacgaccggg aatcgccgga
     3181 agagcctgca gacagaggca cccgcgacga taaaagctca caaaacgaca aaggcgggga
     3241 ctggataggc gagctggagc gggaactgca ggagatcacg aagaagtggc caggcttacg
     3301 gagcctgagc tgaatcatga ccgtaaaaac ccgccatatt tagtagcggg ctgaacgacg
     3361 tagcgcgttc aaaatacaca gcaacgtgcc gaaaccagca tggaacggca cacttaaagt
     3421 gtgtaataaa aaggcgcttc cgcaagtcca ccgcagaccg cacagggcaa aagaaaggct
     3481 ccccgcagcg ggagccttca gcacatacag cagcagtatc ggtttatgca cctctggtga
     3541 ggcacactac cgttatcgac cttctctgca ggcacataag cgcagctgcc atcttttttc
     3601 ttcgtacctg ccgggcgtca cgcttttaac ataacatctc ttggccgcac ccccgcagag
     3661 ctcctcagcg cgtttctgcc gcgtccgggc cgtttccggc acctttcccc ggcccaaacc
     3721 ctgaaaaacg cccctgataa tttctgcgcg gctgcatggc tatgcacaag agtgaatggc
     3781 ggccggtttc gtgaaggaaa taccgtgaaa gctccgaaag aaacgcttaa ttgcacagca
     3841 gcacatgaac gtgatcagat gaaatctgtc tactgccgca cttcttcacg aaaggcccgc
     3901 tgagcgtcag attcgggcct ttcttactaa aaagatttca atagaatttt ttagcaggcg
     3961 atttttatct ataaccatac attgagcata gaagacccaa atttatgtat gactcaatag
     4021 aaaaaaacaa atcacagcca tctaaacact gagacagcaa gccaaacaaa aatcaaagaa
     4081 ataactccat ttttaatcaa ataatatagt tttcgattcc ttttttttag cttctgacct
     4141 gcatcacgaa tagcattaac atatttaaat aaatgattta tagcaccagt cctgtcatta
     4201 ttatcgttgg gtgatgctgc cgctgacatt agcaccccac ctatccacct atttattttt
     4261 tgtgaccacc gccacttcat cggcctttct atatcacaga acaaaataat acgtgtctgc
     4321 tcggtatcat tctgagccca gtgaacaaaa gtttcgtcaa aaattatcac ttcaccatca
     4381 tgccaactgc ggcgtacacc atcaacttca ataaaacacc gttcatcatt tggtgttgat
     4441 aatcctaaat gataacgaac tgaaccggca taagggtcac gatgctttcc taaatggctt
     4501 cctgaaggca attcagcaaa catcgctgct ttaatacagg gaatagagct aaccagttgg
     4561 gttgttactg ggcaaagccg ctccgctgat ggatgagcat ctccatacca cttgaggtaa
     4621 aagcgcttcc aaccgcgctt aaaaaatgta ttaaatcctg catcattatt tttttcagat
     4681 gatttaatgt gcccttgcaa attaacagct tcgtctctta taacctgcca gttatcaatt
     4741 aatggttgaa gttcaggaaa tgtttctggg ggaaaccatg cctgctttga cggcagcttc
     4801 gaaaagcctg tcataaacat atttatcgga gccataaatg ttgaatgatc aaataactgc
     4861 ctggaaaact tttgttttat tttaccgcga gaatgcgcat acaatacact gataagaaaa
     4921 acaaaaataa taataagtga cagcatg
//
