LOCUS       NZ_CP115008             2511 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET22-969 plasmid pCUVET22-969.4,
            complete sequence.
ACCESSION   NZ_CP115008
VERSION     NZ_CP115008.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262096
            Assembly: GCF_030863745.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Nextseq
            500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,585
            CDSs (total)                      :: 4,467
            Genes (coding)                    :: 4,385
            CDSs (with protein)               :: 4,385
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 27 of 82
            Pseudo Genes (incomplete)         :: 54 of 82
            Pseudo Genes (internal stop)      :: 17 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET22-969"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET22-969.4"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2022-06-13"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(308..493)
                     /locus_tag="O8I26_RS22915"
                     /old_locus_tag="O8I26_22885"
     CDS             complement(308..493)
                     /locus_tag="O8I26_RS22915"
                     /old_locus_tag="O8I26_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032105038.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYTMLNAQTGEEDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(860..1087)
                     /locus_tag="O8I26_RS22920"
                     /old_locus_tag="O8I26_22890"
     CDS             complement(860..1087)
                     /locus_tag="O8I26_RS22920"
                     /old_locus_tag="O8I26_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1515..1658)
                     /locus_tag="O8I26_RS22925"
                     /old_locus_tag="O8I26_22895"
     CDS             complement(1515..1658)
                     /locus_tag="O8I26_RS22925"
                     /old_locus_tag="O8I26_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032610644.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHVKTPLAAATGKGYTRLLVQ
                     SS"
                     /name="hypothetical protein"
     Typing          182..261
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.67e-34"
     Typing          complement(1494..2054)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.163"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ttacatttcg tcctgagcat tttctgtatt tctgtccata cttaaacgag gcggccgtca
       61 cggacgatgg tcgtcgttgc atgatgctgt tatggatgag tgaaagtaat ctaatctaac
      121 ttgacttacc ttccgggcag ctccccggtt tttttttgcc tgttcccggg gaaccactca
      181 ggacaggatg tgtatttgta ataccgcgca cgcgccggca tcacaaatac ccaggtcagg
      241 gctgcgcccc gacaccccgc catcgcctca cggccgcgca ctgaattgcg cacccgttca
      301 ccgattttca cgcatcttcc tcgcctgtct gtgcgttcag catggtgtaa agtgcttcgg
      361 cctgatcgtg caggtgctca cagcagccgg cagcctcatc cagatccatc tgttcgagcc
      421 gctcgaggag gaggagtgtc tgtgagcgga taaatttggc catgttgagg gcggcggatt
      481 cctgtctggt cactggtgat tttccccgtc tggtgatgat ttttgcgtga aaagagcatg
      541 ccgtcattct ggtgcatagt catgcatgcc gttaaaattt aacaggagcg tttctggcgg
      601 gttccggggt ggtttgttgt ggtttttggt catggttccg tcagaaaagc gctgagtgcg
      661 tctgaggcgg tgcgggcaac ggatgttatg gtaaattctt ggggggcttg attataaaaa
      721 ccgcacttcc tctaatgtct tgtgcttttt agacaaccct ggaagctttt agctttggtg
      781 gtgcaatttg ctgggatttt ggcggagaac cccaggtaaa gaaaaaggcc acattagcgg
      841 cctttttcgg agaagatgct cagtgcggaa tttcggagac ttctttaacg tcggttttat
      901 tgatctgctg tttgacacca ttcgcgtctg tatagccaag caggcctgag tctgattctt
      961 taggtttgcc gtcgctgaca atagtgcgcc cgtcattggt gtgtatggca tagcttgtgc
     1021 gcgtgcagcc ggtgagggct gaaagcgcaa ctgccgctgc gaacacggaa acaaaaatct
     1081 ttttcaaagc cagctccttt tatccatatc agttttggtc actaaccatc taactatagg
     1141 tcatttttta tgtgaaaaga ggcttgatgg tgggctgcat atcgagttag gtgcgaggtg
     1201 ttatggttaa agtgtggtgt ctggcgggga gggggcagtg cgggcttata tctgatgtaa
     1261 tccgaccgat acatatacca agatgattta agtattcctc tttgtgaggt tggatgtcta
     1321 atttataaaa ggatcttctt gagatccttt tttttcgcgc gtaatctctt gccctgtaaa
     1381 cgaaaaaacc acccggcaag gtggtttttc gaaggttcag caaaccggca agtttctgaa
     1441 ccgtggtaac agggtgtaca agaccgctgc caccaaattt gtccttctag tgtagccgca
     1501 gtcaggcgtc aacttcaaga actctgcacc agtaatcttg tatacccctt accagtggcc
     1561 gctgccagtg gcgtttttac gtgtctttcc gggttggact caagacgata gttaccggat
     1621 aaggcgcagc agtcggactg aacggggggt tcgtgcatac agtccatcct ggagcgaact
     1681 gccttcccgg aaccgagtgt caggcgtgga atgagaaacc gcggccataa cagcggagtg
     1741 acaccggtaa accgaaaggc aggaatgcgg gggagcacga gggagccatc agggggaaac
     1801 gcctggtatc tttaagccgc atcgggtttc gccaccactg atttgagcgt cagatttcgt
     1861 gatgctcgtc aggggggcgg agcctatgga aaaacggctt cggcgcggcc ttatgccttc
     1921 ttcgctaagt atcttcttgg catcccctag ctggcatccc ccaggaaatt tctgctccac
     1981 ccgtaagccc gtaccgctcg ccgcagccga acgaccgagc gcagcgagtg agtgagcgag
     2041 gaagcggaat atatcctgta ttacattttc tcctgacgcg ttttctttca ttttctgcgc
     2101 ctgtcttatg tagcagttat gctatgcgtt actgcggtgt tacattttag ccagtataca
     2161 ctccgctagc gctgagtgac tggtccggcg ctgcgcccgg aacccgcctg taccggttca
     2221 gcagccgttc cgggctgact gcaatttttt tattttcctg cccgctaccc tgtaaacctt
     2281 tcttctgcgt tgccgttaac aggaggtaag ccatgtttgg tttttcttcc ggagacctga
     2341 tgactcttga tgatgagcga cacctgtttg tgtctgttgt ttcgggctgg ctccggcgtg
     2401 ccggggggat gccggagcca tgatgctgga tgcataccgg cagattttgt ctgaaactga
     2461 accggccgta aggacggtca tgctcgaatt tcttgaatcc gttcgcattc a
//
