LOCUS       NZ_CP115007             4760 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET22-969 plasmid pCUVET22-969.3,
            complete sequence.
ACCESSION   NZ_CP115007
VERSION     NZ_CP115007.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262096
            Assembly: GCF_030863745.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Nextseq
            500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,585
            CDSs (total)                      :: 4,467
            Genes (coding)                    :: 4,385
            CDSs (with protein)               :: 4,385
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 27 of 82
            Pseudo Genes (incomplete)         :: 54 of 82
            Pseudo Genes (internal stop)      :: 17 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET22-969"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET22-969.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2022-06-13"
                     /collected_by="Pattrarat Chanchaithong"
     gene            191..406
                     /locus_tag="O8I26_RS22885"
                     /old_locus_tag="O8I26_22855"
     CDS             191..406
                     /locus_tag="O8I26_RS22885"
                     /old_locus_tag="O8I26_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            677..1591
                     /gene="lpxO"
                     /locus_tag="O8I26_RS22890"
                     /old_locus_tag="O8I26_22860"
     CDS             677..1591
                     /gene="lpxO"
                     /locus_tag="O8I26_RS22890"
                     /old_locus_tag="O8I26_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            1991..2452
                     /locus_tag="O8I26_RS22895"
                     /old_locus_tag="O8I26_22865"
     CDS             1991..2452
                     /locus_tag="O8I26_RS22895"
                     /old_locus_tag="O8I26_22865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2707..2817)
                     /locus_tag="O8I26_RS22900"
                     /old_locus_tag="O8I26_22870"
     CDS             complement(2707..2817)
                     /locus_tag="O8I26_RS22900"
                     /old_locus_tag="O8I26_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            3461..3784
                     /locus_tag="O8I26_RS22905"
                     /old_locus_tag="O8I26_22875"
     CDS             3461..3784
                     /locus_tag="O8I26_RS22905"
                     /old_locus_tag="O8I26_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3993..>4427
                     /locus_tag="O8I26_RS22910"
                     /old_locus_tag="O8I26_22880"
                     /pseudo=""
     CDS             3993..>4427
                     /locus_tag="O8I26_RS22910"
                     /old_locus_tag="O8I26_22880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3174..3357
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.631"
                     /coverage="86.0"
                     /evalue="1.25e-50"
     Typing          complement(2708..3191)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          2..504
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.529"
                     /coverage="99.0"
                     /evalue="6.29e-76"
     Typing          3775..4757
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.646"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcagcagata ctcgacaatt acgtgagcat ccgggagcag aagagcacgc aggcgatgct
       61 gtccgagcgg aacagcggcg tgcagctctc gacctgcggc gagcagagac gccgctgcgt
      121 gagggtgaac ccggaagcgg gacggttcgg agaggattcg agctggatga tactggcggg
      181 gaaatagcac atgacagagc tggaaaaaca gttgctgagc gcattagagc agctacagca
      241 ggactactcg aaaaggctgg acgagtggga gagcgccttc gcggaatggc ggacgatgtc
      301 tggtcttatg caacgggaga acgcggcgct gagcgagcgc gtcacgggct tgagcaggca
      361 ggtgcagagt ttgagcgatc agctgcgccg gttgtcgagc ggctgaatgc cgtcgagctg
      421 cagcaggtgc gggaggcgcg gcatcagaaa gcgctggagc tcgaacgatc acagcgacag
      481 cgggtatatc acggtccgtc cctttgattc atttcagcat aattcctgct gaaatcctct
      541 ctgcctgtgt agtagatatg ttctggtgaa gcttcgatta tactattgat aaatagcatt
      601 tagattataa tttaagtgca ttcattatgt tatctttaag agatttttat tattggcgtg
      661 aacaaggaat atacacatgc tgtcacttat tattattttt gtttttctta tcagtgtatt
      721 gtatgcgcat tctcgcggta aaataaaaca aaagttttcc aggcagttat ttgatcattc
      781 aacatttatg gctccgataa atatgtttat gacaggcttt tcgaagctgc cgtcaaagca
      841 ggcatggttt cccccagaaa catttcctga acttcaacca ttaattgata actggcaggt
      901 tataagagac gaagctgtta atttgcaagg gcacattaaa tcatctgaaa aaaataatga
      961 tgcaggattt aatacatttt ttaagcgcgg ttggaagcgc ttttacctca agtggtatgg
     1021 agatgctcat ccatcagcgg agcggctttg cccagtaaca acccaactgg ttagctctat
     1081 tccctgtatt aaagcagcga tgtttgctga attgccttca ggaagccatt taggaaagca
     1141 tcgtgaccct tatgccggtt cagttcgtta tcatttagga ttatcaacac caaatgatga
     1201 acggtgtttt attgaagttg atggtgtacg ccgcagttgg catgatggtg aagtgataat
     1261 ttttgacgaa acttttgttc actgggctca gaatgatacc gagcagacac gtattatttt
     1321 gttctgtgat atagaaaggc cgatgaagtg gcggtggtca caaaaaataa ataggtggat
     1381 aggtggggtg ctaatgtcag cggcagcatc acccaacgat aataatgaca ggactggtgc
     1441 tataaatcat ttatttaaat atgttaatgc tattcgtgat gcaggtcaga agctaaaaaa
     1501 aaggaatcga aaactatatt atttgattaa aaatggagtt atttctttga tttttgtttg
     1561 gcttgctgtc tcagtgttta gatggctgtg atttgttttt ttctattgag tcatacataa
     1621 atttgggtct tctatgctca atgtatggtt atagataaaa atcgcctgct aaaaaattct
     1681 attgaaatct ttttagtaag aaaggcccga atctgacgct cagcgggcct ttcgtgaaga
     1741 agtgcggcag tagacagatt tcatctgatc acgttcatgt gctgctgtgc aattaagcgt
     1801 ttctttcgga gctttcacgg tatttccttc acgaaaccgg ccgccattca ctcttgtgca
     1861 tagccatgca gccgcgcaga aattatcagg ggcgtttttc agggtttggg ccggggaaag
     1921 gtgccggaaa cggccctcag gccgcagaaa cgcgctgagg ggctctcagg gggtgcggcc
     1981 aagaagcgtt atggtaaata catattatgg ttatttatta ggagtaactc tgggagagtg
     2041 ggctatggga tcagatgttc aatggcagat agataatggt tatttggcgc aagttatggg
     2101 ggatttttcg acagagtcgc ctagctttga aacgccaatg tttgggcgat attttgatac
     2161 ctttcaagct gctatacatg ttgcaaaagc tttcccaggg agtacgttgc agccatcaga
     2221 gtacgaagat aaaactggga tgctaatacg tcattattat gtggattgtc atccgtatta
     2281 tgatcgactc cctcctgaaa aaagcgataa ttttcagtat gatgagtacc cttctgctat
     2341 tcatgtggct gcgtgtattg atcaagctag caaagaactc gacgaactat tgtttggtgg
     2401 tggatatggg gaaaatggtg aggggcatgc aagcccgata attttatatt agtgttaggg
     2461 gcggagggat attatccaat ttgtaaaatg atcttcttga gatcgttttg ctctgcgcgt
     2521 aatctcttgc tctgaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta
     2581 agttggggaa cttctgaacc gtggtaacag tcttgtgcga gacgtgtcac caaatctgtc
     2641 cttccagtgt agccgtagtc cggccaccac ttcaagaact ctcgatacat ctctcgcaca
     2701 tcctgcttac cagtggctac cgccaatggc gattttgtcg tgtctttccg ggttggactc
     2761 aagttgatag ttaccggaat gggcgtggta gtcggactga acgggggggt cgtgcataca
     2821 gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac
     2881 gcggccacat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     2941 gagggagcta ccagggggaa acgcctggga tctttatagt cctgtcgggt ttcgccgcca
     3001 ctgatttgag cgtcatattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     3061 caacgccgca tccttttccc agcccttcct gatttgagcg ccatctcttt atcccgccat
     3121 tcgtgagccg gtaacgcccg ccgcagtctc acggcaggag cgtagcgacc gagtgagcga
     3181 ggaggcgtca tttcatcgtc cccagcattt cgcacttacg ccccacagac gcatttttta
     3241 cctgccacat gaagcacttc cctgaattcc cacttcgatt taccatatta agccagtata
     3301 cactccgcta gcgctacgtg actggttcag ggctgcgccc cgaaacccgc taaaaccact
     3361 gacgcgcctg cggcttgtcc aacaccgcgc cgaccgggaa aagatttccc gcccgtcccg
     3421 gcgttatcag gaggccggat tggcagacaa acgcagcaaa atgctcacga tgtgggtgac
     3481 cgaggatgag caccggcgtc tgctggagcg ctgcgacggt aaacagcttg cagcctggat
     3541 gcgccagacg tgcctggacg agaaacccgc ccgcgccggc aagcttccct cgatctcgcc
     3601 ggcgctgctt cgtcagcttg ccggcatggg gaacaacctt aaccagattg cccggcaggt
     3661 taacgccggt ggtggcagcg ggcacgaccg cgtgcaggtc gtcgccgcgc tgatggccat
     3721 agatgccgga ctcgagcggc tgcggcatgc cgtgctggaa aagggtgcag acgatgatcg
     3781 ttaagtttca cccgcgaggg cgcggcggtg gcgccggtcc ggtggattat ctgctgggga
     3841 aagaccgaca gcgcgagggc gccaccgttc tgcaggggaa gccggaagaa gtcagggagc
     3901 ttatcgacgc ctcaccctac gcaaaaaagt acacatccgg cgtcctgtcc tttgccgaaa
     3961 aggatttacc gcccgggcag cgcgaaaagc tgatggcgag ctttgaacgg gttctgatgc
     4021 ccggactcga caaagaccag tacagcgtgc tgtgggttga gcaccaggat aaggggcggc
     4081 tggagctgaa cttcctgatc ccgaacacgg agctgctgac tggcaagcgt ctccagccgt
     4141 attacgaccg cgccgaccgt ccgcgcattg atgcctggca gaccatcgtg aacggcaggc
     4201 tggggctgca tgacccgaac gcgcctgaaa accggcgggc gctggtcacg ccgtccgcgc
     4261 tgccggaaac gaagcaggaa gccgctcagg caattacacg gggtttactg gccttggcct
     4321 cgtccgggga gctgaaaacc cgccaggacg tcactgaggc gctggaaagc gcaggttttg
     4381 aggtggtacg caccacaaaa agcagcatca gcattgccga cccggacggg gggcgaaaca
     4441 tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     4501 aaatcgaaag cgcagcagca gagtaccggc gagatgctga aagccgcatt cagcgagcac
     4561 gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc cgccatcctc
     4621 gaccacgacc ggaagctgtc ctcagccatg agccagcgta cgaaagggat gctgcgcatg
     4681 gtcagccaga cgtggctgac catcgtcctg gtctccgtgc tgctgatcgc ctcgaacgcg
     4741 gccattctgt ggtggcagag
//
