LOCUS       NZ_CP115006             5413 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET22-969 plasmid pCUVET22-969.2,
            complete sequence.
ACCESSION   NZ_CP115006
VERSION     NZ_CP115006.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262096
            Assembly: GCF_030863745.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-AUG-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Nextseq
            500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,585
            CDSs (total)                      :: 4,467
            Genes (coding)                    :: 4,385
            CDSs (with protein)               :: 4,385
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 27 of 82
            Pseudo Genes (incomplete)         :: 54 of 82
            Pseudo Genes (internal stop)      :: 17 of 82
            Pseudo Genes (multiple problems)  :: 15 of 82
            Pseudo Genes (short protein)      :: 1 of 82
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET22-969"
                     /isolation_source="blood"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET22-969.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2022-06-13"
                     /collected_by="Pattrarat Chanchaithong"
     gene            233..448
                     /locus_tag="O8I26_RS22855"
                     /old_locus_tag="O8I26_22825"
     CDS             233..448
                     /locus_tag="O8I26_RS22855"
                     /old_locus_tag="O8I26_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_086581678.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFEEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1504..2085
                     /locus_tag="O8I26_RS22860"
                     /old_locus_tag="O8I26_22830"
     CDS             1504..2085
                     /locus_tag="O8I26_RS22860"
                     /old_locus_tag="O8I26_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247170836.1"
                     /translation="MNNSLILLIFFNKLVSIDKRETTMSARARKAGKRNQSERRQDLIE
                     KLWEDDEIDTWHRLENDGYSTVPRYLPLIGDLMDGLSKGSPLSTTYLALWFRVSDEGLI
                     EIRDKAALAFESGFASERGVTTWAGRMKKLKELGFISCREGSTGEFHYVLIVHPLVAVK
                     KLLDEGIIPKGKTYNILSERVIEVGASWEG"
                     /name="hypothetical protein"
     gene            2086..2580
                     /locus_tag="O8I26_RS22865"
                     /old_locus_tag="O8I26_22835"
     CDS             2086..2580
                     /locus_tag="O8I26_RS22865"
                     /old_locus_tag="O8I26_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000929317.1"
                     /translation="MAGTVWRDIRTGETVFPMGHLHPQRCCVDVNGTSVDIEISFGFHV
                     FTDEKQTGMLMKFKEEQRFFCRERYEGSKTIVHRILTAIENGEYITAFISKGQGQRYYH
                     LSHHDDFILMEIRKPQDRNNSLRIHVVTAYTLDEWGTVNKGRNLRFRYVLEQRLQGKKI
                     V"
                     /name="hypothetical protein"
     gene            2853..3179
                     /locus_tag="O8I26_RS22870"
                     /old_locus_tag="O8I26_22840"
     CDS             2853..3179
                     /locus_tag="O8I26_RS22870"
                     /old_locus_tag="O8I26_22840"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_248217953.1"
                     /translation="MKLLDSNEVCELLGISKSTLYRWCGVSEDFSGLGLRSGLPQANAL
                     NSRLAKATQKSLGWENMLVGRALQQKALGVEETPSDFPRPFKIGRSFKWKDAEIMAWLE
                     TRRV"
                     /name="helix-turn-helix domain-containing protein"
     gene            4147..4470
                     /locus_tag="O8I26_RS22875"
                     /old_locus_tag="O8I26_22845"
     CDS             4147..4470
                     /locus_tag="O8I26_RS22875"
                     /old_locus_tag="O8I26_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_306676585.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDKKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGDTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4679..>5113
                     /locus_tag="O8I26_RS22880"
                     /old_locus_tag="O8I26_22850"
                     /pseudo=""
     CDS             4679..>5113
                     /locus_tag="O8I26_RS22880"
                     /old_locus_tag="O8I26_22850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          3859..4032
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(3387..3892)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.905"
                     /coverage="99.0"
                     /evalue="7.96e-151"
     Typing          2..546
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.568"
                     /coverage="100.0"
                     /evalue="2.69e-91"
     Typing          4148..4464
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="5.79e-60"
     Typing          4461..5413
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.855"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcacgagc gggagcattc tgtggtggca ggggcagcag ataaccgaca attacacgca
       61 tcttcgtcag caggaagata ccctggcgaa aatgaccgcc cggacgtggg gagtgcgcta
      121 tcaggagagc agcgacggcc ggaggtttct tatcctgccg ccggggatgc aaaccgaagc
      181 catcccttac gacgggacga catggatccg cctgaaacag gagtagcgga aaatgacaga
      241 gctggaaaag cagttgctga gcgcattaga gcagctacag caggactact cgcaaaggct
      301 ggacgagtgg gagagcgcct tcgaggaatg gcggagcatg tgtgggctta tgcaacggga
      361 gaacgcgatg ctgaacgagc gcgtgtcgga cttgagcacg caggtgctga gtttaagcga
      421 gcagctgcgc cgcttgtccg ggaactgaat gccatcgagg agaaggctgt actggagtat
      481 cagcgtcaga acgagctgga gctgacgctc cgacagaagc agaagagttt cgatggtccg
      541 aagatgaggt gacagcccgc acgaagaagg gctcatcgcg ttcctgctaa ataggctgac
      601 taccggaatg gtagtgactt atcccggagg aagtgatgaa aattctcaac gttattgaag
      661 ccctgctgaa ctgtttttac ctggtgatgg agctgtgtga gtttctggag aggcatttgc
      721 cggtttttaa ggcgttcgcg ctgattttag tgtgacttta cgcgccatgc atctggctgc
      781 atgactatgc agcagtgtaa tatccagcgg ctgcgtttcc ggtgtttttt cgtcatgtct
      841 gtcgccactt tctcgcgtct ggcaccggaa acgcagccaa ccccgtttca gcggtgcggg
      901 caacggatgt tatggtaaat attggtgctc tgggatgaag cgaaaatagg ctttttttcg
      961 ctgttttagc ctgttcagga acggcaagag taaatatctg ggagcatgaa gctcgttaac
     1021 ctgaatggag atcctgatga tggacaaaga taccaccacc ctaaaacgca ctttggctca
     1081 taatcgagcc ttaatcgata gtattaaccg cagtggcata gcctggtgct ataacactga
     1141 gattgtactc gctgcttgtg aggcgattga agccgaacta caacggcgag gatgtttgta
     1201 acaataagaa aacccgcatc agcgggtttt ttagtctctg agacgcgcta ccgctaaatt
     1261 atttttctct ctatccgccc tggcttgccc cctacaaccc catggagcac aaggagcaca
     1321 gggagaacca tcgagcacac cgagcacaac gagcaccgcc ttcctcttgt aaatcattat
     1381 taatcatata attaagaaac ttcattgtaa tatatattac tttttatgtt tttaaatgta
     1441 atgtttattg cgatttagct tgtttttaac tctatttttt cagatgagaa caagctgcca
     1501 gtcatgaata actctttgat tttattgatt ttttttaata aattagtgtc catagacaaa
     1561 cgggagacga ctatgtccgc aagagcaaga aaagcaggta agagaaatca gtcagaacga
     1621 cgtcaggatt tgattgaaaa attgtgggaa gacgacgaaa tcgatacttg gcatcgactt
     1681 gaaaatgatg ggtactcaac agttccacgt tatttgcctt tgataggcga tctcatggat
     1741 ggtctgtcaa aaggatcacc attatcaaca acctacttgg ctttatggtt cagggtttct
     1801 gatgagggtt tgattgaaat aagagataaa gcagcactgg cttttgaatc agggttcgct
     1861 agtgaaagag gcgtaactac atgggcaggg agaatgaaaa agctaaaaga actggggttc
     1921 atatcctgtc gtgaaggcag caccggtgaa tttcattacg tgttaattgt tcacccatta
     1981 gttgcagtca aaaagctgct tgatgaaggg attatcccta aaggaaagac ttataacatt
     2041 ttgtcggagc gtgtaattga ggtgggagcc tcttgggagg gttaaatggc gggcacagta
     2101 tggcgtgaca taagaacggg agagactgtt tttcctatgg ggcacctgca ccctcagagg
     2161 tgctgtgttg atgtaaacgg tacttctgta gatatagaaa ttagttttgg ttttcatgtt
     2221 tttactgatg aaaaacaaac cggaatgctg atgaaattta aggaggaaca gaggtttttt
     2281 tgtagggaac gctatgaggg cagcaaaact atcgttcaca gaatacttac agcgatagaa
     2341 aacggtgaat acattacagc atttatttca aaggggcagg ggcagcggta ttaccatctt
     2401 agccatcatg atgacttcat tttgatggaa ataagaaagc ctcaagatag aaataattct
     2461 ttgcgtattc atgttgttac agcttacacg cttgatgaat gggggactgt aaataaagga
     2521 cgtaacctgc ggtttagata tgtgctggaa cagcgactgc agggaaagaa gattgtgtaa
     2581 aaaacaaaaa gcgcctaagg cgcctttgtt ttttgcagaa tttcggtctg gcctttcgtt
     2641 atcccgctca caggctggga gggtgtgacc cccggcatca gcacccctct ctcgaggttc
     2701 cggtttgtgc tgacaacatt agtatcctat tccacatgtt cgttgtcaaa ctagcgtact
     2761 ttttggtgtt cattgatgtt tataggttgt catttaccat ctttggggtt ataagtacct
     2821 ataggttttc attgtttttt ataggtgttc atatgaagtt gcttgatagt aatgaagtgt
     2881 gtgagctctt aggcatcagt aagtccactt tgtatcgttg gtgcggggtg tctgaggatt
     2941 tttctggctt gggtctacgt agcggtcttc ctcaggctaa cgctttgaat agccggctgg
     3001 ctaaggctac tcagaaatct ttaggctggg aaaatatgct cgttgggagg gcattacagc
     3061 aaaaagctct cggtgtcgaa gagacgccaa gcgacttccc tcgtcctttc aagatcggtc
     3121 gttcgtttaa atggaaagac gccgaaatta tggcttggct tgaaacgcga cgcgtttgat
     3181 tgatagatct tcttgagatc atttggatct cgtcgtagtc ttttgtcctg taaacgaaaa
     3241 aaccaccctg gcaggtggtt tttcgaaggt tatttaatcc tggcagattg aataaccgtg
     3301 gtaacagtct tgtgcgagac gtgtcaccaa atctgtcctt tcagtgtagc ctcactaagg
     3361 ccaccacttc aagaactctg aatacatctc tcgcacatcc tgtttaccaa tggctaccgc
     3421 cactggcgat ttttcgtgtc tgttccgggt tggactcaag atgatagtta ccgggacaac
     3481 gtggtagtcg ggctgaacgg ggggttcttg cacacagccc agcttggagc gaactgtcta
     3541 aacggaacgg gacgtggtga tttaggtaaa gcttccacca caacacggat gccgcaggac
     3601 gggaacagga gagcgcaaga gggagccgcc agggggaaac gcctggtatc tttaagtcct
     3661 gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg
     3721 agcctatgga aaaacggctt cgccgcggcc ttatcgtttc cccgttaagt ctcttcctgg
     3781 catctaccag gaaatttccg ccccgttcgt aagccatttc cgctcgccgc agccgaacga
     3841 ccgagcgcag cgagtcagtg agcgaggaag cggaatatat cctgtatcgc atattctgct
     3901 gacgcaccgg tgccgccttt tttctcccgc cacatgaagc acttcactga cattcgcatc
     3961 cgtgccaaca tagtaagcca gtatacactc cgctagcgct gcgtgactgg ttcagggctg
     4021 cgccccgaaa cccgctaaaa gccactgacg cgcctgcggc ttgtccaacc ccgcgccgac
     4081 cgggaaaggc tttcccgccc gtcccggcgt tatcaggagg ccggtttgtc agagaaacgg
     4141 aacaagatgc tcaccatgtg ggtgacggag gacgagcacc ggcggctgct tgagcgctgc
     4201 gacggcaggc agctggcagc gtggatgcgg cagacctgcc tggacaaaaa gccggcacgt
     4261 tccggaaagc tcccctcact ctcgccggcg ctgctgcgcc agcttgccgg catgggcaat
     4321 aacctgaatc agatagcccg ccgggttaac gccggcggcg acaccggcca tgacagggta
     4381 cagattgtgg cggcgctgat ggccatcgat gccgggcttg agcggctgcg gcacgccgtt
     4441 cttgagaaag gaacggacga tgatcgttaa atttcatcca cgcggtcgcg gcggtggcgc
     4501 cgggccggtg gactatctgc tgggtaaaga ccggcagcgt gaaggcgcca gcgtcctgca
     4561 ggggaagccg gaagaagtcc gggagctgat tgacgcgtcg ccttacgtga agaaatacac
     4621 ctccggcgtc ctgtcgtttg cggaggccga tctgcctccc ggccagcggg aaaagctgat
     4681 ggccagcttc gagcgggttc tgatgcccgg actcgataaa gatcagtaca gcattctgtg
     4741 ggtggagcac gcggacaagg ggcgtctgga gctgaacttt ctgatcccca ataccgagct
     4801 gctgaccggc aggcggctcc agccgtacta cgaccgcgcc gaccgaccgc gcatcgatgc
     4861 ctggcagacc atcgtgaacg gcagactggg gctgcacgac ccgaacgcac cggaaaaccg
     4921 ccgggcgctg gtcacgccgt ccggactgcc gaaaacgaag caggaggccg cagaagccat
     4981 cacgcgcgga ctgctgaccc ttgcttcgtc cggggagctg aaaagccgtc aggatgtcac
     5041 tgaggcgctg gaacggtcag gttttgaggt ggtgcgcacc acgaaaagca gtatcagcat
     5101 tgccgacccg gacggggggc gaaacatccg actgaaggga gccatctatg agcagtcttt
     5161 taacgctggc gaaggactta gagcagcaat cgaaagcgcg gcagcagagt accggcgaga
     5221 tgctgaaagc cgcattcagc gagcacgagc agtctgtcag agcggaactg agcgcaagcg
     5281 cgaggagaat cagcgacgcc atcctcgccc acgagcagag tatgagcgag gcgatggaga
     5341 aaaaccggcg gagcgtactg cggactgccg ggcggacatg gctcaccatc ctgatggtgt
     5401 cagcgctgct gat
//
