LOCUS       NZ_CP115003             2495 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET22-793 plasmid pCUVET22-793.3,
            complete sequence.
ACCESSION   NZ_CP115003
VERSION     NZ_CP115003.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262095
            Assembly: GCF_030863725.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-OCT-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Nextseq
            500
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:47:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,023
            CDSs (total)                      :: 4,909
            Genes (coding)                    :: 4,813
            CDSs (with protein)               :: 4,813
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 37 of 96
            Pseudo Genes (incomplete)         :: 60 of 96
            Pseudo Genes (internal stop)      :: 17 of 96
            Pseudo Genes (multiple problems)  :: 16 of 96
            Pseudo Genes (short protein)      :: 1 of 96
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP115003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET22-793"
                     /isolation_source="abdominal fluid"
                     /host="cat"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET22-793.3"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2022-05-09"
                     /collected_by="Pattrarat Chanchaithong"
     gene            1090..1317
                     /locus_tag="O8I27_RS25105"
                     /old_locus_tag="O8I27_25090"
     CDS             1090..1317
                     /locus_tag="O8I27_RS25105"
                     /old_locus_tag="O8I27_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1683..1868
                     /locus_tag="O8I27_RS25110"
                     /old_locus_tag="O8I27_25095"
     CDS             1683..1868
                     /locus_tag="O8I27_RS25110"
                     /old_locus_tag="O8I27_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2159..2377)
                     /locus_tag="O8I27_RS25115"
                     /old_locus_tag="O8I27_25100"
     CDS             complement(2159..2377)
                     /locus_tag="O8I27_RS25115"
                     /old_locus_tag="O8I27_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1916..2003)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          147..675
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gggcgcagcg ccggaccagt cacggagcac tagcggagtg tatactggct taagatgtag
       61 catggcagta acacatagca ttaatgccac ataagacagg cgcagaaagt gaaagaaaac
      121 gcgtcaggag aaaatgtgat acagaatata ttccgcttcc tcgctcactg actcgctccg
      181 ctcggtcgtt cggctgcggc gagcgggacg ggcttacgga tggatcagaa atttcctggg
      241 ggatgccagg aagatactta acgaagaaag cataaggccg cgccaaagcc gtttttccat
      301 aggctccgcc cccctgacaa gcatcacaga atctgacgct caaatcagtg gtggcgaaac
      361 ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca
      421 ttcctgcctt tcggtttacc ggtgtcactc cgctgttatg gccgcggttt ctcattccac
      481 gcctgacact cagttccggg aaggcagttc gctccaggat ggactgtatg cacgaacccc
      541 ccgttcagtc cgaccgctgc gccttatccg gtaactatca acttgagtcc aacccggaaa
      601 gacacgacta atcgccattg gcaacggcca ttggcaaaca ggatgtgcga gagatgtctc
      661 aagagttctt gaagtggcgg ccttagtgag gctacactga aaggacaaat ttggtgacat
      721 gtctcgcaca agactgttac cacggttagg ggatctgcca ggattaccta accttcgaaa
      781 aaccacctgc cagggtggtt ttttcgttta cagggcaaga gattacgcgc ggaaaaaaag
      841 gatctcaaga agatccttta taaattagac atccaacctc acaaagagga atacttaaat
      901 catcttggta tatgtatcgg ccggattaca tctcatataa gcctcctccc cgcctgacac
      961 cacactttga ccatagcacc tcgcgcctaa ctcgatatac agcccaccat caagcctctt
     1021 ttcacataaa aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag
     1081 gagctggctt tgaaaaagat ttttgtttca gtgttcgcag cggcagttgc gctttcagcc
     1141 ctcaccggct gcacgcgcac aagctatgcc atacacacca atgacggtcg cactattgtc
     1201 agcgacggca aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt
     1261 gtcaaacagc agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca
     1321 tcttctccga aaaaggccgc taatgtggcc tttttcttta cctgtggttc tccgccaaaa
     1381 tcccagcaaa ttgcatcacc aaagctaaaa gctttcaggg ttgtctaaaa agcacaagac
     1441 ataagaggaa gtgcggtatt ttataatcaa gcccccaaga atttaccata acatccgttg
     1501 cccgcaccgc ctgagacgcg ttcagcgcgt tcctgacgaa accatgacaa aaaccacaac
     1561 aaaccacccc ggaacccgtc agaaacgcgc ctgttaaatt ttaacggcat gcatgactat
     1621 gcaccagaat gacgccatgc tcttttcacg caaaaatcat caccagacgg ggaaaatcac
     1681 cagtgaccag acaggaatcc gccgccctca atatggccaa atttatccgc gcacagacac
     1741 ttctcctcct tgagcggctc gagcagatgg atctggatga ggctgccggc tgctgtgagc
     1801 acctgcacga tcaggccgaa gcgctttacg ccatgctgaa cgcacagata ggcgaggaaa
     1861 atgcgtgaaa atcggtgaac gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg
     1921 gtgtcggggc gcagccctga ccagggtatt tgtgatgccg gcgcgtgcgc ggtattacaa
     1981 atgcacatcc tgtcccggaa cggacaccgg gaaacagcaa aaaaaaccgg gcggcacgcc
     2041 cggaactcaa tcaagttaga ttagattact ctcactcgtc cataacagca tcatggaacg
     2101 acgaccaccg tccgtgacgg ccgcctcgtt taagtatgga cagaaataca gaaaatgctc
     2161 aggacgaaat gtaatgaatg cgaacggatt caagaaattc gagcatgaca gtccttacgg
     2221 ccggttcggt ttcagacaaa atctgccggt atgcatccag catcatggct ccggcatccc
     2281 ctccggcacg ccgtagccag accgaaacaa cggacacaag caggtgtcgc tcatcatcac
     2341 taagagtcat cagggctccg gaagaaaaac caaacatggc ttacctactg ttaacggcaa
     2401 cgcagaagaa aggtttacag ggtagcgggc agggatgaaa aaaaaaaaat tgcagtcagg
     2461 ccggaacggc tgctgaaccg gtacaggcgg gttcc
//
