LOCUS       NZ_CP114999             2496 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET21-1726 plasmid pCUVET21-1726.2,
            complete sequence.
ACCESSION   NZ_CP114999
VERSION     NZ_CP114999.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262094
            Assembly: GCF_030863705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:38:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,603
            CDSs (total)                      :: 4,485
            Genes (coding)                    :: 4,393
            CDSs (with protein)               :: 4,393
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 92
            CDSs (without protein)            :: 92
            Pseudo Genes (ambiguous residues) :: 0 of 92
            Pseudo Genes (frameshifted)       :: 30 of 92
            Pseudo Genes (incomplete)         :: 61 of 92
            Pseudo Genes (internal stop)      :: 17 of 92
            Pseudo Genes (multiple problems)  :: 14 of 92
            Pseudo Genes (short protein)      :: 1 of 92
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114999.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-1726"
                     /isolation_source="blood"
                     /host="cat"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET21-1726.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-11-29"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(2487..2496,1..209)
                     /locus_tag="O8I22_RS23005"
                     /old_locus_tag="O8I22_22970"
     CDS             join(2487..2496,1..209)
                     /locus_tag="O8I22_RS23005"
                     /old_locus_tag="O8I22_22970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     gene            complement(507..692)
                     /locus_tag="O8I22_RS23010"
                     /old_locus_tag="O8I22_22975"
     CDS             complement(507..692)
                     /locus_tag="O8I22_RS23010"
                     /old_locus_tag="O8I22_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1058..1285)
                     /locus_tag="O8I22_RS23015"
                     /old_locus_tag="O8I22_22980"
     CDS             complement(1058..1285)
                     /locus_tag="O8I22_RS23015"
                     /old_locus_tag="O8I22_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          373..460
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="100.0"
                     /evalue="1.5000000000000001e-30"
     Typing          2208..2377
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.116"
                     /coverage="98.0"
                     /evalue="9.61e-56"
     Typing          complement(1701..2283)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 tttcctccgg agccctgatg acttttaatg atgatgagcg acacctgctt gtgtccgttg
       61 tttcgggctg gctccggcgt gccgaagggg atgccggagc catgatgctg gatgcatacc
      121 ggcagatttt gtctgaaacc gaaccggccg caagggcggt catgctcgaa tttcttgaat
      181 cagttcgcat tcattacgtt tcgtcctgag catttcctga agcatttcct gaatttttgt
      241 ccatactcaa tccaggcggc cgtcacggac ggtggtcgtc gttccatgat gctgttatgg
      301 aagattgaga gtaatctaac ttgattgagt tccgggcgtg tcgcccggtt ttttttgctg
      361 tttcccggtg tccgctcccg gacaggatgt gcatttgtaa taccgcgcac gcggcggcat
      421 cacaaatacc ccgttcaggg ctgcgccccg acaccccgcc atcgcctcac ggccgcgcac
      481 tgaattgcgc acccgttcac cgattttcac gcatctttct cgcctatctg tgcgttcagc
      541 atggcgtaaa gcgcttcagc ctgatcgtgc agatgctcac agcagccggc tgcctcatcc
      601 agatccatct gttcgagccg ttcgaggagg aggagtgtct gtgagcggat aaatttggcc
      661 atgttgaggg cggcggattc ctgtctggtc actggtgatt ttccccgtct ggtgatgatt
      721 tttgcgtgaa aagagcatgc cgtcattctg gtgcatagtc atgcatgccg ttaaaattta
      781 acaggcgcgt ttctgacggg ttccggggtg gtttgttgtg gtttttgtca tggttccgtc
      841 agaaacgcgc tgagcgcgtc tgaggcggtg cgggcaacgg atgttatggt aaattcttgg
      901 gggcttgatt ataaaatacc gcacttcctc ttatgtcttg tgctttttag acaaccctga
      961 aagcttttag ctttggtgat gcaatttgct gggattttgg cggagaacca caggtaaaga
     1021 aaaaggccac attagcggcc tttttcggag aatatgctca gtgcggaatt tcggagactt
     1081 ctttaacgtc ggttttattg atctgctgtt tgacaccatt cgcgtctgta tagccaagca
     1141 ggcctgagtc tgattcttta ggtttgccgt cgctgacaat agtgcgaccg tcattggtgt
     1201 gtatggcata gcttgtgcgc gtgcagccgg tgagggctga aagcgcaact gccgctgcga
     1261 acactgaaac aaaaatcttt ttcaaagcca gctcctttta tccatatcag ttttggtcac
     1321 taaccatcta actataggtt attttttatg tgaaaagagg cttgatggtg ggctgtatat
     1381 cgagttaggc gcgaggtgct atggtcaaag tgtggtgtca agcggggagg aggcttatat
     1441 gagatgtaat ccagcagata catataccaa gatgatttaa gtattcctct ttgtgaggtt
     1501 ggatgtctaa tttataaagg atcttcttga gatccttttt tttcgcgcgt aatctcttgc
     1561 cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttaggta atcctggcag
     1621 atcccctaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatttgt cctttcagtg
     1681 tagcctcact aaggccgcca cttcaagaac tcttgataca tctctcgcac atcctgttta
     1741 ccaatggccg ttgccaatgg cgattagtcg tgtctttccg ggttggactc aagttgatag
     1801 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccatcctg
     1861 gagcgaactg ccttcccgga actgagtgtc aggcgtggaa tgagaaaccg cggccataac
     1921 agcggagtga caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag ggagccacca
     1981 gggggaaacg cctggtatct ttaagccgca tcgggtttcg ccaccactga tttgagcgtc
     2041 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgtggcct
     2101 tatgcctttt tcgctaagaa ccttcctggc attctccagg aaatttctgc tctgtacgta
     2161 agcccgtacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2221 ggaatatctc ctgtatcaaa tattctgctg acgcaccgat gccgcctttt tctcctgcca
     2281 catgaagcac ttaactgaca tccgcatccg tgccaacata ttaagccagt atacactccg
     2341 ctagcgctgc gtgactggtc cggcgctgcg cccggaaccc gcctgtaccg gttaagcagc
     2401 cgttctggtc tgactgcgat ttttttcatt cctgcccgct accctgtaaa cctttcttct
     2461 gcgttgccgt taacaggagg taagccatgt ttggtt
//
