LOCUS       NZ_CP114996             2496 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET21-1190 plasmid pCUVET21-1190.2,
            complete sequence.
ACCESSION   NZ_CP114996
VERSION     NZ_CP114996.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262093
            Assembly: GCF_030863685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:23:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,388
            CDSs (with protein)               :: 4,388
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 31 of 95
            Pseudo Genes (incomplete)         :: 63 of 95
            Pseudo Genes (internal stop)      :: 17 of 95
            Pseudo Genes (multiple problems)  :: 14 of 95
            Pseudo Genes (short protein)      :: 1 of 95
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114996.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET21-1190"
                     /isolation_source="abdominal fluid"
                     /host="cat"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET21-1190.2"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2021-08-27"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(93..278)
                     /locus_tag="O8I23_RS22995"
                     /old_locus_tag="O8I23_22960"
     CDS             complement(93..278)
                     /locus_tag="O8I23_RS22995"
                     /old_locus_tag="O8I23_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032673404.1"
                     /translation="MTRQESAALNMAKFIRSQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEKDA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(644..871)
                     /locus_tag="O8I23_RS23000"
                     /old_locus_tag="O8I23_22965"
     CDS             complement(644..871)
                     /locus_tag="O8I23_RS23000"
                     /old_locus_tag="O8I23_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2073..2291
                     /locus_tag="O8I23_RS23005"
                     /old_locus_tag="O8I23_22970"
     CDS             2073..2291
                     /locus_tag="O8I23_RS23005"
                     /old_locus_tag="O8I23_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_182407767.1"
                     /translation="MFGFSSGALMTFNDDERHLLVSVVSGWLRRAEGDAGAMMLDAYRQ
                     ILSETEPAARAVMLEFLESVRIHYVSS"
                     /name="hypothetical protein"
     Typing          1794..1963
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.116"
                     /coverage="98.0"
                     /evalue="9.61e-56"
     Typing          complement(1287..1869)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.331"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 cggcatcaca aataccccgt tcagggctgc gccccgacac cccgccatcg cctcacggcc
       61 gcgcactgaa ttgcgcaccc gttcaccgat tttcacgcat ctttctcgcc tatctgtgcg
      121 ttcagcatgg cgtaaagcgc ttcagcctga tcgtgcagat gctcacagca gccggctgcc
      181 tcatccagat ccatctgttc gagccgttcg aggaggagga gtgtctgtga gcggataaat
      241 ttggccatgt tgagggcggc ggattcctgt ctggtcactg gtgattttcc ccgtctggtg
      301 atgatttttg cgtgaaaaga gcatgccgtc attctggtgc atagtcatgc atgccgttaa
      361 aatttaacag gcgcgtttct gacgggttcc ggggtggttt gttgtggttt ttgtcatggt
      421 tccgtcagaa acgcgctgag cgcgtctgag gcggtgcggg caacggatgt tatggtaaat
      481 tcttgggggc ttgattataa aataccgcac ttcctcttat gtcttgtgct ttttagacaa
      541 ccctgaaagc ttttagcttt ggtgatgcaa tttgctggga ttttggcgga gaaccacagg
      601 taaagaaaaa ggccacatta gcggcctttt tcggagaata tgctcagtgc ggaatttcgg
      661 agacttcttt aacgtcggtt ttattgatct gctgtttgac accattcgcg tctgtatagc
      721 caagcaggcc tgagtctgat tctttaggtt tgccgtcgct gacaatagtg cgaccgtcat
      781 tggtgtgtat ggcatagctt gtgcgcgtgc agccggtgag ggctgaaagc gcaactgccg
      841 ctgcgaacac tgaaacaaaa atctttttca aagccagctc cttttatcca tatcagtttt
      901 ggtcactaac catctaacta taggttattt tttatgtgaa aagaggcttg atggtgggct
      961 gtatatcgag ttaggcgcga ggtgctatgg tcaaagtgtg gtgtcaagcg gggaggaggc
     1021 ttatatgaga tgtaatccag cagatacata taccaagatg atttaagtat tcctctttgt
     1081 gaggttggat gtctaattta taaaggatct tcttgagatc cttttttttc gcgcgtaatc
     1141 tcttgccctg taaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt taggtaatcc
     1201 tggcagatcc cctaaccgtg gtaacagtct tgtgcgagac atgtcaccaa atttgtcctt
     1261 tcagtgtagc ctcactaagg ccgccacttc aagaactctt gatacatctc tcgcacatcc
     1321 tgtttaccaa tggccgttgc caatggcgat tagtcgtgtc tttccgggtt ggactcaagt
     1381 tgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     1441 atcctggagc gaactgcctt cccggaactg agtgtcaggc gtggaatgag aaaccgcggc
     1501 cataacagcg gagtgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag
     1561 ccaccagggg gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg
     1621 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg
     1681 tggccttatg cctttttcgc taagaacctt cctggcattc tccaggaaat ttctgctctg
     1741 tacgtaagcc cgtaccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1801 ggaagcggaa tatctcctgt atcaaatatt ctgctgacgc accgatgccg cctttttctc
     1861 ctgccacatg aagcacttaa ctgacatccg catccgtgcc aacatattaa gccagtatac
     1921 actccgctag cgctgcgtga ctggtccggc gctgcgcccg gaacccgcct gtaccggtta
     1981 agcagccgtt ctggtctgac tgcgattttt ttcattcctg cccgctaccc tgtaaacctt
     2041 tcttctgcgt tgccgttaac aggaggtaag ccatgtttgg tttttcctcc ggagccctga
     2101 tgacttttaa tgatgatgag cgacacctgc ttgtgtccgt tgtttcgggc tggctccggc
     2161 gtgccgaagg ggatgccgga gccatgatgc tggatgcata ccggcagatt ttgtctgaaa
     2221 ccgaaccggc cgcaagggcg gtcatgctcg aatttcttga atcagttcgc attcattacg
     2281 tttcgtcctg agcatttcct gaagcatttc ctgaattttt gtccatactc aatccaggcg
     2341 gccgtcacgg acggtggtcg tcgttccatg atgctgttat ggaagattga gagtaatcta
     2401 acttgattga gttccgggcg tgtcgcccgg ttttttttgc tgtttcccgg tgtccgctcc
     2461 cggacaggat gtgcatttgt aataccgcgc acgcgg
//
