LOCUS       NZ_CP114993             2495 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET19-891 plasmid pCUVET19-891.8,
            complete sequence.
ACCESSION   NZ_CP114993
VERSION     NZ_CP114993.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262092
            Assembly: GCF_030863665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 76 of 138
            Pseudo Genes (incomplete)         :: 65 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            Pseudo Genes (short protein)      :: 1 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114993.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET19-891"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET19-891.8"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2019-05-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            complement(join(2281..2495,1..4))
                     /locus_tag="O8I28_RS25060"
                     /old_locus_tag="O8I28_25040"
     CDS             complement(join(2281..2495,1..4))
                     /locus_tag="O8I28_RS25060"
                     /old_locus_tag="O8I28_25040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            1212..1439
                     /locus_tag="O8I28_RS25065"
                     /old_locus_tag="O8I28_25045"
     CDS             1212..1439
                     /locus_tag="O8I28_RS25065"
                     /old_locus_tag="O8I28_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1805..1990
                     /locus_tag="O8I28_RS25070"
                     /old_locus_tag="O8I28_25050"
     CDS             1805..1990
                     /locus_tag="O8I28_RS25070"
                     /old_locus_tag="O8I28_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2038..2125)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          269..797
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 acatggctta cctactgtta acggcaacgc agaagaaagg tttacagggt agcgggcagg
       61 gatgaaaaaa aaaaaattgc agtcaggccg gaacggctgc tgaaccggta caggcgggtt
      121 ccgggcgcag cgccggacca gtcacggagc actagcggag tgtatactgg cttaagatgt
      181 agcatggcag taacacatag cattaatgcc acataagaca ggcgcagaaa gtgaaagaaa
      241 acgcgtcagg agaaaatgtg atacagaata tattccgctt cctcgctcac tgactcgctc
      301 cgctcggtcg ttcggctgcg gcgagcggga cgggcttacg gatggatcag aaatttcctg
      361 ggggatgcca ggaagatact taacgaagaa agcataaggc cgcgccaaag ccgtttttcc
      421 ataggctccg cccccctgac aagcatcaca gaatctgacg ctcaaatcag tggtggcgaa
      481 acccgatgcg gcttaaagat accaggcgtt tccccctggt ggctccctcg tgctcccccg
      541 cattcctgcc tttcggttta ccggtgtcac tccgctgtta tggccgcggt ttctcattcc
      601 acgcctgaca ctcagttccg ggaaggcagt tcgctccagg atggactgta tgcacgaacc
      661 ccccgttcag tccgaccgct gcgccttatc cggtaactat caacttgagt ccaacccgga
      721 aagacacgac taatcgccat tggcaacggc cattggcaaa caggatgtgc gagagatgtc
      781 tcaagagttc ttgaagtggc ggccttagtg aggctacact gaaaggacaa atttggtgac
      841 atgtctcgca caagactgtt accacggtta ggggatctgc caggattacc taaccttcga
      901 aaaaccacct gccagggtgg ttttttcgtt tacagggcaa gagattacgc gcgaaaaaaa
      961 aggatctcaa gaagatcctt tataaattag acatccaacc tcacaaagag gaatacttaa
     1021 atcatcttgg tatatgtatc ggccggatta catctcatat aagcctcctc cccgcctgac
     1081 accacacttt gaccatagca cctcgcgcct aactcgatat acagcccacc atcaagcctc
     1141 ttttcacata aaaaatgacc tatagttaga tggttagtga ccaaaactga tatggataaa
     1201 aggagctggc tttgaaaaag atttttgttt cagtgttcgc agcggcagtt gcgctttcag
     1261 ccctcaccgg ctgcacgcgc acaagctatg ccatacacac caatgacggt cgcactattg
     1321 tcagcgacgg caaacctaaa gaatcagact caggcctgct tggctataca gacgcgaatg
     1381 gtgtcaaaca gcagatcaat aaaaccgacg ttaaagaagt ctccgaaatt ccgcactgag
     1441 catcttctcc gaaaaaggcc gctaatgtgg cctttttctt tacctgtggt tctccgccaa
     1501 aatcccagca aattgcatca ccaaagctaa aagctttcag ggttgtctaa aaagcacaag
     1561 acataagagg aagtgcggta ttttataatc aagcccccaa gaatttacca taacatccgt
     1621 tgcccgcacc gcctgagacg cgttcagcgc gttcctgacg aaaccatgac aaaaaccaca
     1681 acaaaccacc ccggaacccg tcagaaacgc gcctgttaaa ttttaacggc atgcatgact
     1741 atgcaccaga atgacgccat gctcttttca cgcaaaaatc atcaccagac ggggaaaatc
     1801 accagtgacc agacaggaat ccgccgccct caatatggcc aaatttatcc gcgcacagac
     1861 acttctcctc cttgagcggc tcgagcagat ggatctggat gaggctgccg gctgctgtga
     1921 gcacctgcac gatcaggccg aagcgcttta cgccatgctg aacgcacaga taggcgagga
     1981 aaatgcgtga aaatcggtga acgggtgcgc aattcagtgc gcggccgtga ggcgatggcg
     2041 gggtgtcggg gcgcagccct gaccagggta tttgtgatgc cggcgcgtgc gcggtattac
     2101 aaatgcacat cctgtcccgg aacggacacc gggaaacagc aaaaaaaacc gggcggcacg
     2161 cccggaactc aatcaagtta gattagatta ctctcactcg tccataacag catcatggaa
     2221 cgacgaccac cgtccgtgac ggccgcctcg tttaagtatg gacagaaata cagaaaatgc
     2281 tcaggacgaa atgtaatgaa tgcgaacgga ttcaagaaat tcgagcatga cagtccttac
     2341 ggccggttcg gtttcagaca aaatctgccg gtatgcatcc agcatcatgg ctccggcatc
     2401 ccctccggca cgccgtagcc agaccgaaac aacggacaca agcaggtgtc gctcatcatc
     2461 actaagagtc atcagggctc cggaagaaaa accaa
//
