LOCUS       NZ_CP114992             2758 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET19-891 plasmid pCUVET19-891.7,
            complete sequence.
ACCESSION   NZ_CP114992
VERSION     NZ_CP114992.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262092
            Assembly: GCF_030863665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 76 of 138
            Pseudo Genes (incomplete)         :: 65 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            Pseudo Genes (short protein)      :: 1 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114992.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET19-891"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET19-891.7"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2019-05-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            join(2609..2758,1..273)
                     /locus_tag="O8I28_RS25035"
                     /old_locus_tag="O8I28_25015"
     CDS             join(2609..2758,1..273)
                     /locus_tag="O8I28_RS25035"
                     /old_locus_tag="O8I28_25015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080052137.1"
                     /translation="MTITYLALIFITLLTINIILKNRRSKKYLAYNICLAQFTYENEST
                     ENRARIENKAKEIIANGSSIQHMKNTIKQNNPLVIYGFYALAMDELGVPPLLYGFDRWY
                     PVRNPFLAEHGLSRHIEKAVDKIENKSKVQWEHWIN"
                     /name="hypothetical protein"
     gene            296..586
                     /locus_tag="O8I28_RS25040"
                     /old_locus_tag="O8I28_25020"
     CDS             296..586
                     /locus_tag="O8I28_RS25040"
                     /old_locus_tag="O8I28_25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013813157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_080052136.1"
                     /translation="MKKITIAALSLVLLSGCASVSKSYGPDGNEAYSLTCSGMARDWGM
                     CLTKAGELCGAKGYNILSINGDSGALVTANPQSLMAGSVINRNMLISCKQL"
                     /name="lipoprotein"
     gene            817..1005
                     /locus_tag="O8I28_RS25045"
                     /old_locus_tag="O8I28_25025"
     CDS             817..1005
                     /locus_tag="O8I28_RS25045"
                     /old_locus_tag="O8I28_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165998.1"
                     /translation="MTKQESAALNMAKFIRAQSLLLLEKLDVLDLDDEAADCERMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1053..1280)
                     /locus_tag="O8I28_RS25050"
                     /old_locus_tag="O8I28_25030"
     CDS             complement(1053..1280)
                     /locus_tag="O8I28_RS25050"
                     /old_locus_tag="O8I28_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224019247.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAVMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     gene            2133..2279
                     /locus_tag="O8I28_RS25055"
                     /old_locus_tag="O8I28_25035"
     CDS             2133..2279
                     /locus_tag="O8I28_RS25055"
                     /old_locus_tag="O8I28_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_114456554.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAATGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     Typing          1753..2302
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.918"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 atagaaaata aagccaagga aataatagcc aatggttcat ccattcaaca catgaaaaat
       61 acaatcaaac aaaacaatcc gcttgtgatt tatggctttt acgcattagc aatggatgag
      121 ctaggagtcc cccctttact atatggattt gataggtggt atccagttag aaacccattc
      181 cttgcagaac atgggctgag tcgacatata gaaaaggcag tagacaaaat agaaaataaa
      241 tcaaaagtac aatgggaaca ttggattaat taactttata taaagtaggt gtgaagtgaa
      301 aaagataaca attgcagcat taagtcttgt actactttcc ggctgtgctt cagtaagcaa
      361 aagctatggc ccagatggaa acgaggcgta cagtttaact tgttcaggaa tggcccgtga
      421 ttggggaatg tgtttaacaa aggcaggaga actgtgtgga gcaaaaggat ataacatcct
      481 tagtataaat ggcgattccg gagcattagt tacagccaac ccacaatcat taatggctgg
      541 ctcagtcatc aatagaaaca tgctcatatc ctgcaagcag ctataggaca taaaaagagc
      601 caaccttgag gtacatttac cataacatcc gttgcccgca ccacctcagg cgccctcagg
      661 gcgtttctga ggactccggc ctgaaacata cacctttctg tgcccatatc gagaaaaaac
      721 gcccctgatg atttctgcgt acctgcatgg ctatgcactc aagtgaatga agtaagattt
      781 cagtctgcga tcaccataaa accgggaaaa agccgtatga caaaacagga atcagccgca
      841 ctgaacatgg caaaatttat ccgggcgcag tcgctgctgc tgctcgaaaa actcgacgtg
      901 ctcgatcttg atgacgaggc agccgactgt gagcgaatgc acgaacaggc agaagcgctt
      961 tatcagaagc tgagcgcacg cttcggtata caggacggcg aataaagaga cgaaaagaac
     1021 gctcacttag acgaaaagcg tatccatggc aactatgtat atccaccgtg tacatagtca
     1081 tgcctgacgc tttcgagcag gtcgagcatg taggaacgac gagcttgatt ggtatcggac
     1141 acaatatggc gatacgcctc aaacataaca gcacccgcat caccgcccga acggcgaagc
     1201 atagcggaaa tcatccgcac aaagagcaga cgttcatcgg ggcaaaggtt cataagttct
     1261 cctggtgaaa aaccaaacat gaagcaccca tctattaacg tcagctgtga acgcttcatt
     1321 agaagaaaat aggtaaaccc gggaaaaaga tccaaacgtt aacatcacag gtaaacagaa
     1381 acgtcgggtc gatcgggaaa ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg
     1441 cgtcagtgct tttagcgggt gtcggggcgc agccctgaac cagtcacgta gcgctagcgg
     1501 agtgtatact ggctaaactt cagagatgcc aaggaaggga caggatatgt tttgtagcac
     1561 cgccaccacg gtggcgatac tccaaaacgc ctggtcaggg cacagccccg acaccccaga
     1621 gccgactgta ggtcactggg cgaacgcggc ggaatgatgt cggatgacgt cattttgcgg
     1681 cgttcgccct atcctgcatc gtattaaaaa atgcagctat ggcgcgtacg gtgccaaaga
     1741 taagcccgga gatattccgc ttcctcgctc actgactcgc tccgctcggt cgttcggctg
     1801 cggcgagcgg taccggctta ctggcggggc ggaaatttcc tggcatcttc caggaagaag
     1861 cttaacaggg aagcaagaag gccacggcga agccgttttt ccataggctc cgcccccctg
     1921 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggacttaaag
     1981 ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt
     2041 taccggtgtc attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt
     2101 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgact
     2161 gctgcgcctg ttccggtaac tatcgacttg agtccaaccc ggaaagacac gacaaagcgc
     2221 cactggcagc agccactggt aagagggtgt acaagagatt actgatgcag agttcttgaa
     2281 gtcagagcca aactacggct aaactggaag gacgaatttg gtggcagcgg tcttgtacac
     2341 cctgttacca cggttcagaa gttccccaac ttactgaacc ttcgaaaaac cacctcccca
     2401 ggtggttttt tcgttttcag agcaagagat tacgacgcaa cggaaaggat ctcaagaggt
     2461 tcatcttcga tgaccgacag acatcaaagt aaaaccgata ccaaacaaca gtaccagtca
     2521 aaaactcaca ccgacaaaac cctttgaaaa taaagcataa aaaatcgtat agttggatga
     2581 tgcacaatca ccatttaagc tagtaaaaat gacaatcaca tacttagctc ttatatttat
     2641 caccctacta acaatcaata taatattaaa aaatagacgt tctaaaaaat atttagcata
     2701 taacatctgc cttgctcaat tcacttatga aaatgaatct acagaaaaca gagccaga
//
