LOCUS       NZ_CP114991             3223 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Enterobacter hormaechei strain CUVET19-891 plasmid pCUVET19-891.6,
            complete sequence.
ACCESSION   NZ_CP114991
VERSION     NZ_CP114991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32262092
            Assembly: GCF_030863665.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Leelapsawas,C., Sroithongkham,P., Payungporn,S., Nimsamer,P.,
            Yindee,J., Collaud,A., Perreten,V. and Chanchaithong,P.
  TITLE     First report of blaOXA-181-carrying IncX3 plasmids in
            multidrug-resistant Enterobacter hormaechei and Serratia nevei
            recovered from canine and feline opportunistic infections
  JOURNAL   Microbiol Spectr 12 (3), e0358923 (2024)
   PUBMED   38319115
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Leelapsawas,C., Perreten,V., Collaud,A. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 20-MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina Novaseq
            6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:40:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,014
            CDSs (total)                      :: 4,898
            Genes (coding)                    :: 4,760
            CDSs (with protein)               :: 4,760
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 76 of 138
            Pseudo Genes (incomplete)         :: 65 of 138
            Pseudo Genes (internal stop)      :: 20 of 138
            Pseudo Genes (multiple problems)  :: 20 of 138
            Pseudo Genes (short protein)      :: 1 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP114991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="CUVET19-891"
                     /isolation_source="urine"
                     /host="dog"
                     /db_xref="taxon:158836"
                     /plasmid="pCUVET19-891.6"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2019-05-10"
                     /collected_by="Pattrarat Chanchaithong"
     gene            193..678
                     /locus_tag="O8I28_RS25010"
                     /old_locus_tag="O8I28_24990"
     CDS             193..678
                     /locus_tag="O8I28_RS25010"
                     /old_locus_tag="O8I28_24990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            682..897
                     /locus_tag="O8I28_RS25015"
                     /old_locus_tag="O8I28_24995"
     CDS             682..897
                     /locus_tag="O8I28_RS25015"
                     /old_locus_tag="O8I28_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1065..1397
                     /locus_tag="O8I28_RS25020"
                     /old_locus_tag="O8I28_25000"
     CDS             1065..1397
                     /locus_tag="O8I28_RS25020"
                     /old_locus_tag="O8I28_25000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_016807542.1"
                     /translation="MNTYIFLGGAIVAEVIGTIFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1606..1776)
                     /locus_tag="O8I28_RS25025"
                     /old_locus_tag="O8I28_25005"
     CDS             complement(1606..1776)
                     /locus_tag="O8I28_RS25025"
                     /old_locus_tag="O8I28_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004193911.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="hypothetical protein"
     gene            2415..2738
                     /locus_tag="O8I28_RS25030"
                     /old_locus_tag="O8I28_25010"
     CDS             2415..2738
                     /locus_tag="O8I28_RS25030"
                     /old_locus_tag="O8I28_25010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          2128..2319
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1665..2145)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..995
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.39"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2729..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.364"
                     /coverage="99.0"
                     /evalue="1.57e-102"
ORIGIN
        1 cgaagcagga agccgcccag gcgattacga gcggcctgct tgcccttgcc tcatccgggg
       61 agctgaaaac gcgtcaggac gtcactgagg cgctggaaag cgcaggtttt gaggtggtgc
      121 gcaccacaaa aagcagcatc agcattgccg acccggacgg ggggcgaaac atccgactga
      181 agggagccat ctatgaacag tcttttaacg ctggcgaagg acttagagca gaaatcgaaa
      241 gcgcagcagc agactaccgg cgagatgctg aaagccgcat tcagcgagca cgagaagtct
      301 gtcagagcgg aactgagcga aagcgagaag agaatcagcg ccgccatcct cgaccacgac
      361 cggaagctgt cctcagccat gagccagcgc acgaaaggga tgctgcgcat ggtcagccag
      421 acgtggctga ccatcgttct ggtctccgcg ctgctgatag cgtcgagcgc gggcattctg
      481 tggtggcagg ggcagcagat actcgagaac tatacgacca tccgggagca gaagagcacg
      541 caggccatgc tgtcagagag gaacagcggc gtacagctct cgacctgcgg cgagcagaga
      601 cgccgctgcg tgagggtgaa cccggaagcg ggacagttcg gagaggactc gagctggatg
      661 atactggcgg ggaaatagca catgacggag ctggaaaaac agttgctgag cgcattagag
      721 cagctacagc aggactactc gaaaaggctg gacgagtggg agaacgcctt cgcggaatgg
      781 cggacgatgt ctggtcttat tcaacgggag aacgcggcgc tgaacgagcg cgtcacggtc
      841 ttgagcaggc aggtgcagag tttgagcgag cagctgcgcc ggttgtcgaa aggctgaacg
      901 cgatagaaac tcaccggcag caggagcggg cggtgcagca ccagaaagcg ctggagctgg
      961 aacgatcgca gtggcagcat catggtcctt cgctatgata agccgggttc tattaattag
     1021 ttgttatgta aagatattta aatttttcaa catgaggtat ttttatgaat acatatattt
     1081 tcctgggagg ggctattgtg gctgaagtca taggtaccat ctttatgaaa tattcagaag
     1141 gatttacacg cctgtggccg tctgtaggaa ctgcaataag ttattgcgct gccttttatc
     1201 tgctttccca aacattggaa aatataccta ccggtattgc ttacgcaatt tggtctggtg
     1261 cgggaattgt actaataagc ctagctggtt ggttggttag cggtcaaaaa cttgattggc
     1321 ccgcaattgc aggaatgttc cttatttgcg ttggagtttt ggttattaat attttttcaa
     1381 aatcttctgc gcattaactt tttaccgaag catattggcc aaagcaaaag gatttccttg
     1441 agatcctttc tttctgcgcg taatcttttt ccctgtaaac aaaaaaacca ccgctaccaa
     1501 cggtggttcg tttgccggaa ttcagagcta ccaactcttt gttccgatgg taactggctt
     1561 tcacggagcg cagataccta aactgtcctt tcagtgtagc cgcactcagg ctttcacttc
     1621 aagaactctg taggcattct ctacaatctc tgctccgtga acccagttac caatggctac
     1681 cgccactggc gatttgtcgt gtctttccgg gttggactca agttgatagt taccggaacg
     1741 ggcgtggtag tcggactgaa cggggggttc gtgcatacag tccatcctgg agcgaactgc
     1801 ctacccggaa ccgagtttca ggcgtggaat gagaaaccgc ggccataaca gcggaatgac
     1861 accggtaaac cgaaaggcag gaatgcgggg gagcacgagg gagccaccag ggggaaacgc
     1921 ctggtatctt taagccgcat cgggtttcgc caccgctgat ttgagcgtca ttttcgtgat
     1981 actcgtcagg ggggcggagc ctatggaaaa acggcaactc cgcatccttt tcttggcctt
     2041 tcctgcttcg agcggcattt gcttatcccg taatctgtga gctggtaacg cccgccgcag
     2101 tctcacggca ggagcgtagc gaccgagtga gcgaggaggc gtcatttcat caccacctgc
     2161 attttgcact tacgcccggc agccgcattt ttcccctgtc acatgaagca ctttcataaa
     2221 ttgccactcc gtcataacat gtttagccag tatacactcc gctagcgcta cgtgactggt
     2281 tcagggctgc gccccgaaac ccgctaaaac cactgccgcg cctgcggctt gtccaacacc
     2341 gtgccgaccg ggaaaagatt tcccgtccgt cccggcgtta tcaggaggcc ggattggcag
     2401 acaaacgcag caaaatgctc accatgtggg tgactgagga cgagcaccgg cgtctgctgg
     2461 agcgctgcga gggtaagcaa ctcgcggcct ggatgcggca gacgtgcctg gacgagaagc
     2521 ccgcacgcgc cggcaaactt ccgtcgatct cgccggcgct gcttcgtcag cttgccggca
     2581 tggggaacaa ccttaaccag attgcccgcc aggttaacgc cggtggtgga agcggacacg
     2641 accgcgtgca gattgtcgcc gcgctgatgg ccatcgatgc cggactcgag cggttgcggc
     2701 atgccgtact ggaaaagggt gctgatgatg atcgttaagt ttcatcctcg ggggcgcggc
     2761 ggcggcggcg ggccggtcga ttatctgctg ggtaaagatc gccagcgcga cggcgccagc
     2821 gttctgcagg ggaagccgga tgaagtccgg gagcttatcg acgcctcgcc ctacgccaaa
     2881 aagtacactt ccggcgttct gtcttttgcc gaacaggatt taccgcccgg ccagcgcgaa
     2941 aagctgatgg cgagtttcga gcgggttctg atgcccggac tcgataaaga ccagtacagc
     3001 gtgctgtggg ttgagcaccg ggacaagggg cggctggagc tgaacttcct gatcccgaac
     3061 acggagcttc tgaccggcaa gcggctacag ccgtattacg accgcgccga ccgtccgcgc
     3121 atcgatgcct ggcagaccat agtgaacggc aggctggggc tgcatgaccc gaacgcgccg
     3181 gagaaccggc gggtgctggt ctcgccttcc gcgctgccgg aag
//
